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The study of biological activity of transformation products of diclofenac and its interaction with chlorogenic acid

Journal of Environmental Sciences 2020年第5期32卷 128-141页

作者： Marzena Matejczyk Piotr Ofman Katarzyna Dabrowska Renata Swislocka Wlodzimierz lewandowski Bialystok University of Technology Faculty of Civil Engineering and Environmental SciencesDepartment of ChemistryBiology and Biotechnology15-341BialystokPoland Bialystok University of Technology Department of Environmental Engineering TechnologyBialystok15-341Poland Waclaw Dabrowski Institute of Agricultural and Food Biotechnology Department of Microbiology02-532WarsawPoland

In the present work we compared the biological activity of DCF,4’-OHDCF and 5-OHDCF as molecules of most biodegradation pathways of DCF and selected transformation products(2-hydroxyphenylacetic acid;2,5-dihydroxyphenylacetic acid and 2,6-dichloroaniline)which are produced during AOPs,such as ozonation and UV/H2 *** also examined the interaction of DCF with chlorogenic acid(CGA).CGA is commonly used in human diet and entering the environment along with waste mainly from the processing and brewing of coffee and it can be toxic for microorganisms included in activated *** the present experiment the evaluation of following parameters was performed:*** K-12 cells viability,growth inhibition of *** K-12 culture,lC50 and mortality of Chironomus aprilinus,ge no toxicity,sodA promoter induction and ROS *** addition the reactivity of *** SM recA:luxCDABE biosensor strain in wastewater matrices was *** results showed the influence of DCF,4’-OHDCF and 5-OHDCF on *** K-12 cells viability and bacteria growth,comparable to AOPs *** highest toxicity was observed for selected,tested AOPs by-products,in comparison to the DCF,4’-OHDCF and *** assay indicated that 2,6-dichloroaniline(AOPs by-product)had the highest toxic *** oxidative stress assays revealed that the highest level of ROS generation and sodA promoter induction were obtained for DCF,4’-OHDCF and 5-OHDCF,compared to other tested *** have also found that there is an interaction between chlorogenic acid and DCF,which resulted in increased toxicity of the mixture of the both compounds to *** K-12,comparable to parent *** strongest response of *** SM biosensor strain with recA:luxCDABE genetic construct in filtered treated wastewaters,comparable to control sample was *** indicates,that *** SM recA:luxCDABE biosensor strains is a good tool for bacteria monitoring in wastewater *** to toxicity and biological

关键词： Diclofenac Biodegradation Chlorogenic acid Advances oxidation processes (AOPs) Wastewaters Microbial biosensors

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The Analytical Framework for the Optimal allocation of Athletes Sustainable livelihood Assets in China

The Analytical Framework for the Optimal Allocation of Athle...

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2011 Chinese Control and Decision Conference（CCDC）

作者： Dexin Zou,is with the Research Center of Sports Social Science,Shenyang Sports University,Shenyang 110102,China Economical College of liaoning University,Shenyang 110036,China Chong Jiang.,wis with Shenyang Sports University,Shenyang 110102,China.

Athelets sustainable livelihoods refers to the living capabilities,assets and income-generating activities that possessed and acquired by athletes in their life cycle to withstand external shock and pressure to improve the long-term living *** reallocation of livelihood assets determines the selection and results of micro livelihoods strategy of retired athletes;the optimal allocation of livelihoods assets is affected by the implementation process of macro-organization,system and policy,which give feedback on the vulnerability of the *** livelihood results give feedback on the allocation of livelihood *** core of the life Cycle-Sustainable livelihoods Framework is livelihoods *** composition of livelihood assets is *** accumulation of human capital,financial capital and social capital are easily damaged under the context of ***,it requires organizations to promote the optimal allocation of livelihoods and to achieve the sustainable livelihoods through the system and policies.

关键词： athletes sustainable livelihood assets optimal allocation

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Genetic down-regulation of gibberellin results in semi-dwarf poplar but few non-target effects on chemical resistance and tolerance to defoliation

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Journal of Plant Ecology 2019年第1期12卷 124-136页

作者： Christine Buhl Steven H.Strauss Richard l.lindroth Department of Entomology University of Wisconsin-Madison1630 Linden DriveMadisonWI 53706USA Department of Forest Ecosystems and Society Oregon State University321 Richardson HallCorvallisOR 97331USA Present address:2600 State Street SalemOR 97310USA

Aims Plant stature can be strongly modified via regulation of endog-enous levels and signalling of the plant hormone gibberellin(GA).Down-regulation of GA can produce semi-dwarf tree varieties with improved qualities such as reduced susceptibility to wind damage,enhanced root growth and more compact ***,these modifications may have unintended,non-target consequences for defence against herbivores,via either of two mechanisms:(i)reduced biomass production may cause trade-offs with chemical resistance traits,as predicted by the growth-differentiation balance hypothesis,and(ii)altered biomass allocation to either roots or pho-tosynthetic tissues may affect regrowth potential and thus tolerance to *** We studied GA down-regulated(GE)and non-transgenic wild-type hybrid poplar(Populus alba×***)in an outdoor,above-ground common garden and defoliated half of all replicate trees to simulate *** then quantified the independent and inter-active effects of genotype and defoliation on growth and chemical resistance-related traits,including phenolic glycosides(PGs),con-densed tannin and *** also calculated tolerance to defo-liation as the differential in relative growth between undefoliated and defoliated *** Findings Our results indicate that two of the four GA down-regulated geno-types had significantly reduced stem height,basal diameter,vol-ume(d2h),total biomass and increased allocation to leaves relative to the wild *** of those two genotypes also had reduced allocation to *** and sometimes both of these same two genotypes also had at least 20%lower levels of condensed tannins and PGs and similar increases in lignin and ***,as calculated by the differential in relative growth between unde-foliated and defoliated trees,was similar among all experimental ***,two GE genotypes flushed fewer leaves in response to defoliation relative to the wild *** results indi-cate

关键词： genetic modification gibberellic acid Populus pest susceptibility tolerance resistance

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一氧化氮对舌下神经核兴奋作用的影响

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华西口腔医学杂志 2012年第3期30卷 317-320页

作者：吴海莺 liu Hattie Horner Richard l 刘流昆明医学院第二附属医院耳鼻喉科昆明650101 多伦多大学医学生理学系昆明医学院第一附属医院整形外科昆明650032

目的观察一氧化氮（NO）及其清除剂对舌下神经运动核（HMN）活动的影响并初步探讨其机制。方法麻醉雄性成年wistar大鼠,微透析探针先透析人工脑脊液（ACSF）到HMN作为对照,然后再分别持续给予二乙胺一氧化氮水合钠盐（DEA,NO供体）和2-... 详细信息

目的观察一氧化氮（NO）及其清除剂对舌下神经运动核（HMN）活动的影响并初步探讨其机制。方法麻醉雄性成年wistar大鼠,微透析探针先透析人工脑脊液（ACSF）到HMN作为对照,然后再分别持续给予二乙胺一氧化氮水合钠盐（DEA,NO供体）和2-（4-羧基苯基）-4,4,5,5,-四甲基-1,3-二氧咪唑啉钾盐（carboxy-PTIO,NO清除剂）到HMN,观测大鼠颏舌肌（GG）、膈肌电活动及血压和呼吸频率的变化。结果给予DEA后,大鼠的呼吸相关GG活动明显增加,而给予carboxy-PTIO后则明显降低,与ACSF时的值相比差异均有统计学意义（P〈0.05）。不管是给予DEA还是carboxy-PTIO,在30~120 min时大鼠的紧张性GG活动、膈肌活动、血压及呼吸频率与ACSF时的值相比均无统计学差异（P〉0.05）。结论 NO作为一种神经递质对HMN产生兴奋,从而对维持生理状态下上气道的开放起重要作用。

关键词：一氧化氮舌下神经核神经递质颏舌肌阻塞性睡眠呼吸暂停综合征

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应激状态下急性酒精中毒大鼠胃黏膜损伤模型的建立

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护理研究（上旬版） 2006年第9期20卷 2272-2274页

作者：马鸿雁孙晓红吕萍吉林大学第一医院

[目的]建立应激状态下急性酒精中毒胃黏膜损伤的动物模型。[方法]采用析因实验设计,将96只wis-tar大鼠分为应激组(A组)、酒精中毒组(B组)、应激后酒精中毒组(C组)、空白对照组(D组)。A组和C组大鼠倒立8h,之后给B组和C组大鼠灌入56度白酒... 详细信息

[目的]建立应激状态下急性酒精中毒胃黏膜损伤的动物模型。[方法]采用析因实验设计,将96只wis-tar大鼠分为应激组(A组)、酒精中毒组(B组)、应激后酒精中毒组(C组)、空白对照组(D组)。A组和C组大鼠倒立8h,之后给B组和C组大鼠灌入56度白酒,直到大鼠出现酒精中毒症状为止。A组、D组大鼠灌入等量生理盐水。分别于酒精中毒后2h、24h、48h处死8只大鼠,观察胃黏膜的病理变化,并进行胃黏膜损伤评分。[结果]B组与A组、C组胃黏膜损伤评分差异均有统计学意义(P<0.05);且不同时间点胃黏膜损伤评分差异亦有统计学意义(P<0.05);不同处理方法和时间因素有交互作用。[结论]倒立8h后酗酒法能成功地建立应激状态下急性酒精中毒大鼠胃黏膜损伤的模型,并出现胃黏膜损伤的征象。

关键词：动物模型大鼠应激急性酒精中毒胃黏膜损伤

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人参皂甙对老龄大鼠大脑皮质AChE老龄性改变的影响

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解剖科学进展 2009年第1期15卷 97-99页

作者：高杰赵海花赖红高旭红李兆圣吕永利中国医科大学解剖教研室辽宁沈阳110001

目的观察大鼠大脑皮质乙酰胆碱酯酶(AChE)阳性纤维的老龄性改变,探讨人参皂甙对其影响。方法24只雌性wis青tar大鼠随机分为青年组、老龄组及给予人参皂甙的给药组。对各组大鼠大脑皮质进行AChE酶细胞化学染色,图像分析各组大鼠大脑皮质... 详细信息

目的观察大鼠大脑皮质乙酰胆碱酯酶(AChE)阳性纤维的老龄性改变,探讨人参皂甙对其影响。方法24只雌性wis青tar大鼠随机分为青年组、老龄组及给予人参皂甙的给药组。对各组大鼠大脑皮质进行AChE酶细胞化学染色,图像分析各组大鼠大脑皮质神经纤维AChE含量及AChE纤维的配布情况。结果老龄组大鼠大脑皮质神经纤维AChE平均光密度值较年组明显减少(p<0.01),AChE纤维面积百分比例下降,人参皂甙组大鼠大脑皮质神经纤维AChE平均光密度值较老龄组明显增加(p<0.01),纤维面积百分比例上升。结论人参皂甙可增加老龄大鼠大脑皮质纤维中AChE平均光密度值,促进纤维侧枝出芽和纤维网的生长及重建。

关键词：大脑皮质乙酰胆碱酯酶人参皂甙老龄大鼠酶细胞化学染色

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