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Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries

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Bioactive Materials 2021年第3期6卷 638-654页

作者： Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang akon higuchi Qingsong Ye School and Hospital of Stomatology Wenzhou Medical UniversityWenzhouZhejiangChina Tianyou Hospital Wuhan University of Science and TechnologyWuhan430064China Skeletal Biology Research Center Massachusetts General Hospital and Harvard School of Dental MedicineBostonMA02114USA Wenzhou Institute of Biomaterials and Engineering WenzhouZhejiang Province325000China Molecular Pharmacology Research Center School of PharmacyWenzhou Medical UniversityWenzhou325035China School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityWenzhouZhejiang325027China Department of Biliary and Pancreatic Surgery/Cancer Research Center Affiliated Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Center of Regenerative Medicine Renmin Hospital of Wuhan UniversityWuhan430060China

Due to the limitations in autogenous nerve grafting or Schwann cell transplantation,large gap peripheral nerve injuries require a bridging strategy supported by nerve conduit.Cell based therapies provide a novel treatment for peripheral nerve injuries.In this study,we first experimented an optimal scaffold material synthesis protocol,from where we selected the 10%GFD formula(10%GelMA hydrogel,recombinant human basic fibroblast growth factor and dental pulp stem cells(DPSCs))to fill a cellulose/soy protein isolate composite membrane(CSM)tube to construct a third generation of nerve regeneration conduit,CSM-GFD.Then this CSM-GFD conduit was applied to repair a 15-mm long defect of sciatic nerve in a rat model.After 12 week post implant surgery,at histologic level,we found CSM-GFD conduit could regenerate nerve tissue like neuron and Schwann like nerve cells and myelinated nerve fibers.At physical level,CSM-GFD achieved functional recovery assessed by a sciatic functional index study.In both levels,CSM-GFD performed like what gold standard,the nerve autograft,could do.Further,we unveiled that almost all newly formed nerve tissue at defect site was originated from the direct differentiation of exogeneous DPSCs in CSM-GFD.In conclusion,we claimed that this third-generation nerve regeneration conduit,CSM-GFD,could be a promising tissue engineering approach to replace the conventional nerve autograft to treat the large gap defect in peripheral nerve injuries.

关键词： Dental pulp stem cells Human basic fibroblast growth factor Gelatin methacrylate Large gap Peripheral nerve injuries Nerve graft

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3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities

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Journal of Materials Science & Technology 2021年第4期63卷 9-17页

作者： Tzu-Cheng Sung Chao-Wen Heish Henry Hsin-Chung Lee Jhe-Yu Hsu Chun-Ko Wang Jia-Hua Wang Yu-Ru Zhu Shih-Hsi Jen Shih-Tien Hsu Abdurahman HHirad Abdullah AAlarfaj akon higuchi School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityNo.270Xueyuan RoadWenzhouZhejiang325027China Department of Chemical and Materials Engineering National Central UniversityTaoyuan 32001Taiwan Department of Surgery Hsinchu Cathay General HospitalHsinchu 30060China Graduate Institute of Translational and Interdisciplinary Medicine National Central UniversityTaoyuan 32001Taiwan Department of Obstetrics and Gynecology Landseed HospitalTaoyuan 32405Taiwan Department of Internal Medicine Landseed HospitalTaoyuan 32405Taiwan Department of Botany and Microbiology King Saud UniversityRiyadh 11451Saudi Arabia Wenzhou Institute University of Chinse Academy of ScienceWenzhou 325001China Center for Emergent Matter Science Riken2-1 HirosawaWakoSaitama 351-0198Japan

Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-saving.However,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension culture.In this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing processes.The hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process alone.Furthermore,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to differentiate.However,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D culture.The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing processes.Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in hASCs.We discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility.

关键词： culture alone stem

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Electroencephalogram Signal Correlations between Default Mode Network and Attentional Functioning

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Journal of Behavioral and Brain Science 2024年第4期14卷 119-134页

作者： Moemi Matsuo Takashi higuchi Toranosuke Abe Takuya Ishibashi Ayano Egashira Rio Kamashita Faculty of Rehabilitation Sciences Nishi Kyushu University Kanzaki Japan Department of Physical Therapy Osaka University of Human Sciences Settsu Japan Research Center for Child Mental Development Chiba University Chiba Japan

Attentional issues may affect acquiring new information, task performance, and learning. Cortical network activities change during different functional brain states, including the default mode network (DMN) and attention network. We investigated the neural mechanisms underlying attentional functions and correlations between DMN connectivity and attentional function using the Trail-Making Test (TMT)-A and -B. Electroencephalography recordings were performed by placing 19 scalp electrodes per the 10 - 20 system. The mean power level was calculated for each rest and task condition. Non-parametric Spearman’s rank correlation was used to examine the correlation in power levels between the rest and TMT conditions. The most significant correlations during TMT-A were observed in the high gamma wave, followed by theta and beta waves, indicating that most correlations were in the parietal lobe, followed by the frontal, central, and temporal lobes. The most significant correlations during TMT-B were observed in the beta wave, followed by the high and low gamma waves, indicating that most correlations were in the temporal lobe, followed by the parietal, frontal, and central lobes. Frontoparietal beta and gamma waves in the DMN may represent attentional functions.

关键词： Cortical Network Activities Electroencephalography Attention Default Mode Network

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Enhancement of Visual Attention by Color Revealed Using Electroencephalography

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Open Journal of Therapy and Rehabilitation 2024年第1期12卷 1-9页

作者： Moemi Matsuo Takashi higuchi Takuya Ishibashi Ayano Egashira Toranosuke Abe Hiroya Miyabara Faculty of Rehabilitation Sciences Nishikyushu University Kanzaki Japan Department of Physical Therapy Osaka University of Human Sciences Settsu Japan

Attention constitutes a fundamental psychological feature guiding our mental effort toward specific objects, concurrent with processes such as memory, reasoning, and imagination. Visual attention, crucial for selecting surrounding information, often decreases in older adults and patients with cerebrovascular disorders. Effective methods to enhance attention are scarce. Here, we investigated whether color information influences visual attention and brain activity during task performance, utilizing EEG. We examined 13 healthy young adults (seven women and six men;mean age: 21.2 ± 0.58 years) using 19-electrode electroencephalograms to assess the impact of color information on visual attention. The Clinical Assessment for Attention cancellation test was conducted under the black, red, and blue color conditions. Wilcoxon’s signed-rank test was used to assess differences in task performance (task time and error) between conditions. Spearman’s rank correlation was utilized to examine the correlation in power levels between task performance and color conditions. Significant variations in total task errors were observed among color conditions. The black condition exhibited the highest error frequency (0.7 ± 0.9 times), followed by the red condition (0.5 ± 0.8 times), with the lowest error frequency occurring in the blue (0.2 ± 0.4 times) condition (black vs. red: P = 0.03;black vs. blue: P = 0.00;red vs. blue: P = 0.032). No time difference was observed. The black condition showed negative delta and high-gamma correlations in the central electrodes. The red condition revealed positive alpha and low-gamma correlations in the frontal and occipital areas. Although no correlations were observed in the blue condition, it enhanced attentional performance. Positive alpha and low-gamma waves might be crucial for spotting attentional errors in key areas. Our findings provide insights into the effects of color information on visual attention and potential neural correlates associated with attentional processes. In conclusion, our

关键词： Attention Higher Brain Function Electroencephalography Neuroimaging Rehabilitation

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CT Thresholds for Spinal Bone Mineral Attenuation to Prioritize Eligible Older Women for Osteoporosis Screening

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Open Journal of Radiology 2024年第2期14卷 92-101页

作者： Seo Yeon K. Orite Bryn higuchi Hyo-Chun Yoon Lana H. Gimber St. Elizabeths Medical Center Brighton MA USA University of California Irvine CA USA Kaiser Permanente Division of Research Oakland CA USA Department of Diagnostic Imaging Hawaii Permanente Medical Group Honolulu HI USA

Introduction: Computed tomography (CT) measurements of bone mineral attenuation may be a useful means to identify older women who should be prioritized for bone mineral density screening. Methods: We compared bone mineral attenuation as measured in the L1 vertebra of CT studies to the results of dual-energy x-ray absorptiometry (DEXA) studies to determine what CT attenuation thresholds might yield a reasonable combination of sensitivity and specificity for the detection of osteoporosis. The study was limited to women between the ages of 65 and 75 years who had a DEXA study and a CT that included the L1 or adjacent vertebra performed within 3 years of the DEXA study. Results: There were 1226 women in this study, of whom 452 (38%) had osteoporosis based on a T-score ≤ −2.5 by DEXA. There were 830 CT studies performed with contrast and 396 studies which were performed without contrast. There was a statistically significant difference in the mean HU of those studies performed without contrast compared to those with contrast (unenhanced mean 103 HU versus 125 HU, p < 0.001). Different CT attenuation thresholds provided the most appropriate combination of sensitivity and specificity for the detection of osteoporosis when comparing CT studies performed without or with IV contrast and when all the CT data were used in aggregate. Conclusion: Different thresholds appear necessary when using the mean CT vertebral attenuation to identify older women for preferential referral for DEXA studies.

关键词： Osteoporosis Bone Mineral Attenuation CT Threshold

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Tumor size discrepancy between endoscopic and pathological evaluations in colorectal endoscopic submucosal dissection

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World Journal of Gastrointestinal Endoscopy 2024年第3期16卷 136-147页

作者： Takeshi Onda Osamu Goto Toshiaki Otsuka Yoshiaki Hayasaka Shun Nakagome Tsugumi Habu Yumiko Ishikawa Kumiko Kirita Eriko Koizumi Hiroto Noda Kazutoshi higuchi Jun Omori Naohiko Akimoto Katsuhiko Iwakiri Department of Gastroenterology Nippon Medical SchoolGraduate School of MedicineBunkyo-ku 113-8603TokyoJapan Endoscopy Center Nippon Medical School HospitalBunkyo-ku 113-8603TokyoJapan Department of Hygiene and Public Health Nippon Medical SchoolBunkyoku 113-8603TokyoJapan Center for Medical Education Nippon Medical SchoolBunkyo-ku 113-8603TokyoJapan

BACKGROUND Tumor size impacts the technical difficulty and histological curability of colorectal endoscopic submucosal dissection(ESD);however,the preoperative evaluation of tumor size is often different from histological assessment.Analyzing influential factors on failure to obtain an accurate tumor size evaluation could help prepare optimal conditions for safer and more reliable ESD.METHODS This was a retrospective study conducted at a single institution.A total of 377 lesions removed by colorectal ESD at our hospital between April 2018 and March 2022 were collected.We first assessed the difference in size with an absolute per-centage of the scaling discrepancy.Subsequently,we compared the clinicopatho-logical characteristics of the correct scaling group(>-33%and33%),which was further subdivided into the underscaling group(-33%or less of the discrepancy)and overscaling group(33%or more of the discrepancy),respectively.As secondary outcome measures,parameters on size estimation were compared between the underscaling and correct scaling groups,as well as between the overscaling and correct scaling groups.Finally,multivariate analysis was performed in terms of the following relevant parameters on size estimation:Pathological size,location,and possible influential factors(P<0.1)in the univariate analysis.RESULTS The mean of absolute percentage in the scaling discordance was 21%,and 91 lesions were considered to be incorrectly estimated in size.The incorrect scaling was significantly remarkable in larger lesions(40 mm vs 28 mm;P<0.001)and less experience(P<0.001),and these two factors were influential on the underscaling(75 lesions;P<0.001).Conversely,compared with the correct scaling group,16 lesions in the overscaling group were significantly small(20 mm vs 28 mm;P<0.001),and the small lesion size was influential on the overscaling(P=0.002).CONCLUSION Lesions indicated for colorectal ESD tended to be underestimated in large tumors,but overestimated in small one

关键词： Endoscopic submucosal dissection Colorectal tumor Tumor size Size estimation Size discrepancy

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有关法人处罚立法的刑法理论考察

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北京社会科学 2024年第1期 59-64页

作者：樋口亮介黎宏(译) 东京大学清华大学法学院

在法人处罚立法上,可以并用法人和自然人同一视的个人模式和追究法人自身责任的组织模式,但在建构法人自身刑事责任方面,应当以过失犯为中心。在法人过失的认定上,仅考虑法人过失就足够了,将企业合规措施单独提出来加以考虑,缺乏积极意... 详细信息

在法人处罚立法上,可以并用法人和自然人同一视的个人模式和追究法人自身责任的组织模式,但在建构法人自身刑事责任方面,应当以过失犯为中心。在法人过失的认定上,仅考虑法人过失就足够了,将企业合规措施单独提出来加以考虑,缺乏积极意义。日本实务中的过失,不是在事前对其内容一概都有规定,而是根据个案的不同情况,斟酌考虑其中的注意义务内容,在法人过失犯罪案件中,必须实质性地考虑合规体制对于法人业务风险防控的意义。在对法人故意犯和过失犯具体分析的基础上,提出日本刑法中法人处罚的完善建议,建议在立法中增设处罚法人具体情形的明确规定。

关键词：法人处罚法人过失法人故意企业合规

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Corrigendum to‘Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries’[Bioactive Mater.6(3)(2021)638-654]

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Bioactive Materials 2022年第1期7卷 2-2页

作者： Lihua Luo Yan He Ling Jin Yanni Zhang Fernando P.Guastaldi Abdullkhaleg A.Albashari Fengting Hu Xiaoyan Wang Lei Wang Jian Xiao Lingli Li Jianming Wang akon higuchi Qingsong Ye School and Hospital of Stomatology Wenzhou Medical UniversityWenzhouZhejiangChina Tianyou Hospital Wuhan University of Science and TechnologyWuhan430064China Skeletal Biology Research Center Massachusetts General HospitalHarvard School of Dental MedicineBostonMA02114USA Wenzhou Institute of Biomaterials and Engineering WenzhouZhejiang Province325000China Molecular Pharmacology Research Center School of PharmacyWenzhou Medical UniversityWenzhou325035China School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityWenzhouZhejiang325027China Department of Biliary and Pancreatic Surgery Cancer Research CenterAffiliated Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Center of Regenerative Medicine Renmin Hospital of Wuhan UniversityWuhan430060China

The authors regret that the author(A.H.)was removed as an agreement could not be reached between the two affiliations of the author.The authors would like to apologise for any inconvenience caused.

关键词： injuries removed repair

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A neuron's ambrosia: non-autonomous unfolded protein response of the endoplasmic reticulum promotes lifespan

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Neural Regeneration Research 2022年第2期17卷 309-310页

作者： Stefan Homentcovschi Ryo higuchi-Sanabria Department of Molecular&Cellular Biology University of CaliforniaBerkeleyCAUSA

The ability to protect cellular components in the face of deleterious conditions, such as exposure to chemical poisons, damaging radiation, or excessive heat, is crucial to organismal viability.

关键词： autonomous delete exposure

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Exploring the type of social support available to aged male hypertensive clients in Ghana

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Frontiers of Nursing 2022年第2期9卷 225-231页

作者： James Konir Zufaa akon Emmanuel Ndiok Irene Korkoi Aboh Philomina Wooley Department of Male Medical Ejura Municipal HospitalEjuraGhana Department of School of Nursing Science Faculty of Allied Medical SciencesUniversity of CalabarCalabarNigeria/Garden City University CollegeKenyase-KumasiGhana Department of Adult Health School of Nursing and MidwiferyUniversity of Cape CoastCape CoastGhana Department of Indexing and Examination Nurses and Midwifery Council of GhanaAccraGhana

Objective: To explores the social support available to aged hypertensive male clients in a municipality in Ghana.Methods: The inclusion criteria were as follows: age ≥60 years and are known hypertensive patients. Data were collected from 186 selected aged male hypertensive patients for 3 months. The questionnaire was self-developed and open-ended. The data were processed and analyzed using SPSS version 23. Some of the variables were subjected to statistical tests and ranks in the order of impor tance to respondents. The enrolled respondents were allowed to par ticipate in the study after their informed consent was obtained. It was evident that respondents do not obtain much support or help in taking care of their condition.Results: The result revealed that the repondents has no support to help take care of their condition as evident by the responses in the study. The scores on average mean for some variables are(AM = 2.25, SD = 0.381) more than the test value of 2.50. Some of these supports were on feeding support(M = 3.97, SD = 0.278, n = 186), health support(M = 2.87, SD = 0.167, n = 186), and cleaning support(M = 2.59, SD = 0.868, n = 186). Supports such as clothing, socialization, medication, washing, transportation, and financial support were lacking.Conclusions: Based on the finding that certain forms of support were lacking, the study concludes that steps undertaken by the government, community, religious bodies, and family toward improving these supports can be of immense help for aged male hypertensive patients living in the Ejura-Sekyedumase municipality.

关键词： aged male Ghana hypertension social support

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