检索结果-南通市图书馆

Generation of glyceraldehyde-derived advanced glycation end-products in pancreatic cancer cells and the potential of tumor promotion

引用

World Journal of Gastroenterology 2017年第27期23卷 4910-4919页

作者： takanobu takata tadashi ueda akiko sakasai-sakai masayoshi takeuchi department of advanced medicine medical research institutekanazawa medical university

To determine the possibility that diabetes mellitus promotes pancreatic ductal adenocarcinoma via glyceraldehyde (GA)-derived advanced glycation-end products (GA-AGEs). METHODSPANC-1, a human pancreatic cancer cell line, was treated with 1-4 mmol/L GA for 24 h. The cell viability and intracellular GA-AGEs were measured by WST-8 assay and slot blotting. Moreover, immunostaining of PANC-1 cells with an anti-GA-AGE antibody was performed. Western blotting (WB) was used to analyze the molecular weight of GA-AGEs. Heat shock proteins 90α, 90β, 70, 27 and cleaved caspase-3 were analyzed by WB. In addition, PANC-1 cells were treated with GA-AGEs-bovine serum albumin (GA-AGEs-BSA), as a model of extracellular GA-AGEs, and proliferation of PANC-1 cells was measured. RESULTSIn PANC-1 cells, GA induced the production of GA-AGEs and cell death in a dose-dependent manner. PANC-1 cell viability was approximately 40% with a 2 mmol/L GA treatment and decreased to almost 0% with a 4 mmol/L GA treatment (each significant difference was P < 0.01). Cells treated with 2 and 4 mmol/L GA produced 6.4 and 21.2 μg/mg protein of GA-AGEs, respectively (P < 0.05 and P < 0.01). The dose-dependent production of some high-molecular-weight (HMW) complexes of HSP90β, HSP70, and HSP27 was observed following administration of GA. We considered HMW complexes to be dimers and trimers with GA-AGEs-mediated aggregation. Cleaved caspase-3 could not be detected with WB. Furthermore, 10 and 20 μg/mL GA-AGEs-BSA was 27% and 34% greater than that of control cells, respectively (P < 0.05 and P < 0.01). CONCLUSIONAlthough intracellular GA-AGEs induce pancreatic cancer cell death, their secretion and release may promote the proliferation of other pancreatic cancer cells.

关键词： Tumor promotion Glyceraldehyde-derived advanced glycation-end products Pancreatic ductal adenocarcinoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bacterial community succession during the enrichment of chemolithoautotrophic arsenite oxidizing bacteria at high arsenic concentrations

引用

Journal of Environmental Sciences 2012年第12期24卷 2133-2140页

作者： Nguyen Ai Le akiko Sato Daisuke Inoue Kazunari Sei Satoshi Soda Michihiko Ike Division of Sustainable Energy and Environmental Engineering Osaka University2-1 YamadaokaSuitaOsaka 565-0871Japan Department of Health Science Kitasato University1-15-1 KitasatoSagamihara-MinamiKanagawa 252-0373Japan

To generate cost-effective technologies for the removal of arsenic from water, we developed an enrichment culture of chemolithoau- totrophic arsenite oxidizing bacteria （CAOs） that could effectively oxidize widely ranging concentrations of As（III） to As（V）. In addition, we attempted to elucidate the enrichment process and characterize the microbial composition of the enrichment culture. A CAOs enrichment culture capable of stably oxidizing As（III） to As（V） was successfully constructed through repeated batch cultivation for more than 700 days, during which time the initial As（iiI） concentrations were increased in a stepwise manner from 1 to 10-12 mmol/L. As（III） oxidation activity of the enrichment culture gradually improved, and 10-12 mmol/L As（III） was almost completely oxidized within four days. Terminal restriction fragment length polymorphism analysis showed that the dominant bacteria in the enrichment culture varied drastically during the enrichment process depending on the As（III） concentration. Isolation and characterization of bacteria in the enrichment culture revealed that the presence of multiple CAOs with various As（Ⅲ） oxidation abilities enabled the culture to adapt to a wide range of As（Ⅲ） concentrations. The CAOs enrichment culture constructed here may be useful for pretreatment of water from which arsenic is being removed.

关键词： arsenic bacterial community chemolithoautotrophic arsenite oxidizing bacteria enrichment terminal-restriction fragmentlength polymorphism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Painting image browser applying an associate-rule-aware multidimensional data visualization technique

引用

Visual Computing for Industry,Biomedicine,and Art 2020年第1期3卷 18-30页

作者： Ayaka Kaneko akiko Komatsu Takayuki Itoh Florence Ying Wang Ochanomizu University 2-1-1 OtsukaBunkyo-ku1128610 TokyoJapan CSIRO Julius Ave.2113 North RydeNSWAustralia

Exploration of artworks is enjoyable but often time *** example,it is not always easy to discover the favorite types of unknown painting *** is not also always easy to explore unpopular painting works which looks similar to painting works created by famous *** paper presents a painting image browser which assists the explorative discovery of user-interested painting *** presented browser applies a new multidimensional data visualization technique that highlights particular ranges of particular numeric values based on association rules to suggest cues to find favorite painting *** study assumes a large number of painting images are provided where categorical information(e.g.,names of artists,created year)is assigned to the *** presented system firstly calculates the feature values of the images as a preprocessing *** the browser visualizes the multidimensional feature values as a heatmap and highlights association rules discovered from the relationships between the feature values and categorical *** mechanism enables users to explore favorite painting images or painting images that look similar to famous painting *** case study and user evaluation demonstrates the effectiveness of the presented image browser.

关键词： Painting image Multi-dimensional data visualization Association rule

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Carbon Nanobelts:Brief History and Perspective

引用

Precision Chemistry 2023年第9期1卷 516-523页

作者： Daiki Imoto akiko Yagi Kenichiro Itami Department of Chemistry Graduate School of ScienceNagoya UniversityNagoya 464-8602Japan Institute of Transformative Bio-Molecules(WPI-ITbM) Nagoya UniversityNagoya 464-8602Japan

Carbon nanotubes(CNTs)are an emerging nanomaterial because of their outstanding performance in various *** recent years,the segment molecules of CNTs,referred to as carbon nanorings(CNRs)or carbon nanobelts(CNBs),have gained attention for their unique structures and properties,as well as their potential as seed molecules for the precise synthesis of *** are rigid and thick segments of CNTs whose synthesis has been addressed by scientists fascinated by the uniqueness of CNBs long before the discovery of *** 60 years of efforts by synthetic chemists all over the world,the synthesis of the first CNB,(6,6)CNB,was achieved by our group in *** this milestone,diverse types of nanobelts have been synthesized through various synthetic routes,thereby demonstrating their photophysical,magnetic,and redox properties derived from rigid belt *** applications of CNBs have also been introduced *** formation of the host−guest complex,transformation to three-dimensional molecules,and measurement of conductivity have been reported for *** paper summarizes the brief history and perspective of *** synthetic campaigns and aggressive application of CNBs would create novel and groundbreaking scenes in materials science.

关键词： carbon nanobelt carbon nanoring carbon nanotube cycloparaphenylene aromatic compounds organic synthesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

c-Jun N-terminal kinase-mediated Rubicon expression enhances hepatocyte lipoapoptosis and promotes hepatocyte ballooning

引用

World Journal of Gastroenterology 2016年第28期22卷 6509-6519页

作者： akiko Suzuki Keisuke Kakisaka Yuji Suzuki Ting Wang Yasuhiro Takikawa division of hepatology department of internal medicine iwate medical university Morioka 0208505 Japan

AIM: To clarify the relationship between autophagy and lipotoxicity-induced apoptosis, which is termed "lipoapoptosis," in non-alcoholic steatohepatitis. METHODS: Male C57BL/6J mice were fed a high-fat diet(HFD) for 12 wk, after which the liver histology and expression of proteins such as p62 or LC3 were evaluated. Alpha mouse liver 12(AML12) cells treated with palmitate(PA) were used as an in vitro model. RESULTS: LC3-Ⅱ, p62, and Run domain Beclin-1 interacting and cysteine-rich containing(Rubicon) proteins increased in both the HFD mice and in AML12 cells in response to PA treatment. Rubicon expression was decreased upon c-Jun N-terminal kinase(JNK) inhibition at both the m RNA and the protein level in AML12 cells. Rubicon knockdown in AML12 cells with PA decreased the protein levels of both LC3-Ⅱ and p62. Rubicon expression peaked at 4 h of PA treatment in AML12, and then decreased. Treatment with caspase-9 inhibitor ameliorated the decrease in Rubicon protein expression at 10 h of PA and resulted in enlarged AML12 cells under PA treatment. The enlargement of AML12 cells by PA with caspase-9 inhibition was canceled by Rubicon ***: The JNK-Rubicon axis enhanced lipoapoptosis, and caspase-9 inhibition and Rubicon had effects that were cytologically similar to hepatocyte ballooning. As ballooned hepatocytes secrete fibrogenic signals and thus might promote fibrosis in the liver, the inhibition of hepatocyte ballooning might provide antifibrosis in the NASH liver.

关键词： Ballooned hepatocyte Caspase 9 c-Jun N-terminal kinase Rubicon SP600125

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Patient blinding with blunt tip placebo acupuncture needles:comparison between 1 mm and 2 mm skin press

引用

Journal of Integrative Medicine 2018年第3期16卷 164-171页

作者： Nobuari Takakura Miho Takayama Morihiro Nasu Masako Nishiwaki akiko Kawase Hiroyoshi Yajima Department of Acupuncture and Moxibustion Faculty of Health Sciences Tokyo Ariake University of Medical and Health Sciences Department of Physiology Showa University School of Medicine Japan School of Acupuncture Moxibustion and Physiotherapy

Objective： To investigate the influence of the depth of skin press in blunt tip placebo acupuncture needles on patient blinding and its relationship to needle ***： Forty healthy volunteers were enrolled as subjects for patient blinding. Four acupuncturists applied the following needles randomly at three points in each forearm： 0.18 mm and 0.25 mm diameter penetrating needles inserted to a depth of 5 mm, and 0.18 mm and 0.25 mm diameter skin-touch needles depressing the skin at the acupoint to a depth of 1 mm and 2 mm from the skin surface. The subjects reported their guesses at the nature of needles they received, and rated needle pain and de qi. A blinding index was calculated to define the success of blinding for ***： The blinding status of subjects for 1 mm press needles of 0.18 mm diameter was ＂random guess＂,but ＂unblinded＂ for 1 mm press needles of 0.25 mm diameter. For 2 mm press needles of both diameters,the blinding status was ＂opposite guess＂ and the blinding status for penetrating needles of both diameters was ＂unblinded.＂ The percentages of ＂felt pain＂ with 2 mm press needles of both diameters were similar to that with penetrating needles, but those were not similar for 1 mm press needles. The frequency of de qi occurrence with 2 mm press needles of 0.18 mm diameter was similar to that of penetrating needles of both ***： Placebo needles of 2 mm press made more subjects guess that the needles penetrated the skin than 1 mm press needles. The use of small diameter needles increased patient blinding.

关键词： Double-blind method Acupuncture Placebo Sham De qi

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Extracellular vesicles in non-alcoholic and alcoholic fatty liver diseases

引用

Liver Research 2018年第1期2卷 30-34页

作者： akiko Eguchi Ariel E.Feldstein Department of Gastroenterology and Hepatology Graduate School of MedicineMie UniversityTsuJapan Division of Gastroenterology Hepatology and NutritionDepartment of PediatricsRady's Children HospitalUniversity of California San DiegoSan DiegoCAUSA

Fatty liver diseases,non-alcoholic fatty liver disease(NAFLD)and alcoholic liver disease(ALD)are the most common causes of chronic liver diseases around the *** and ALD can progress towards a more severe form of the disease,including as non-alcoholic steatohepatitis(NASH)and alcoholic steatohepatitis(ASH).In both instances central pathogenic events include hepatocyte death,liver inflammation,pathological angiogenesis,and fibrosis,followed by cirrhosis and *** the last few years,extracellular vesicles(EVs)have been identified as effective cell-to-cell communicators that contain a cell-and stressspecific cargo from the cell of origin and are capable of transferring this cargo to a target or acceptor *** this review,we focus on the growing evidence supporting a role for EVs in the pathophysiology of NASH and ASH as well as their potential roles as targets for novel biomarkers for these conditions.

关键词： Extracellular vesicles(EVs) Exosomes Microparticles Fatty liver diseases Lipotoxicity Non-alcoholic steatohepatitis(NASH)Alcoholic steatohepatitis(ASH)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Blue-light-induced rapid chloroplast de-anchoring in Vallisneria epidermal cells

引用

Journal of Integrative Plant Biology 2015年第1期57卷 93-105页

作者： Yuuki Sakai Shin-Ichiro Inoue akiko Harada Ken-Ichiro Shimazaki Shingo Takagi Department of Biological Sciences Graduate School of Science Osaka University Division of Biological Science Graduate School of Science Nagoya University Department of Biology Osaka Medical College Department of Biology Faculty of Science Kyushu University

In the outer periclinal cytoplasm of leaf epidermal cells of an aquatic angiosperm Vallisneria, blue light induces ＂chloroplast de-anchoring＂, a rapid decline in the resistance of chloroplasts against centrifugal force. Chloroplast de- anchoring is known induced within lmin of irradiation with high-fluence-rate blue light specifically, preceding the com- mencement of chloroplasts migration toward the anticlinal cytoplasm. However, its regulatory mechanism has remained elusive, although pharmacological analysis suggested that a calcium release from intracellular calcium stores is necessary for the response, in search of the responsible photoreceptors, immunoblotting analysis using antibodies against phototropins demonstrated that cross-reactive polypeptides of 120-kDa exist in the plasma-membrane fraction prepared from the leaves. In vitro phosphorylation analysis revealed that 120-kDa polypeptides were phosphorylated by exposure to blue light in a fluence-dependent manner. The blue-light-induced phosphorylation activity was sensitive to a Ser/Thr kinase inhibitor, staurosporine, and unusually was retained at a high level for a long time in darkness. Furthermore, phototropin gene homologs （Vallisneria PHOTOTROPINI and PHOTOTROPIN2） expressed in leaves were isolated. We propose that calcium- regulated chloroplast de-anchoring, possibly mediated by phototropins, is an initial process of the blue-light-induced avoidance response of chloroplasts in Vallisneria.

关键词： Avoidance response of chloroplasts blue-light response chloroplast de-anchoring cytoplasmic calcium phototropin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular analyses of Xenopus laevis Mesp-related genes

引用

Integrative Zoology 2009年第4期4卷 387-394页

作者： Keisuke HITACHI akiko KONDOW Hiroki DANNO Yusuke NISHIMURA Koji OKABAYASHI Makoto ASASHIMA Department of Life Sciences(Biology) Graduate School of Arts and SciencesThe University of TokyoTokyoJapan Department of Biological Sciences Graduate School of ScienceThe University of TokyoTokyoJapan ICORP Organ Regeneration Project Japan Science and Technology AgencyTokyoJapan Organ Development Research Laboratory National Institute of Advanced Industrial Science and TechnologyIbarakiJapan

During vertebrate somitogenesis,somites bud off from the anterior end of the presomitic mesoderm(PSM).Meso-dermal posterior(Mesp)-related genes play essential roles in somitogenesis,particularly in the definition of the somite boundary *** vertebrates,two types of Mesp-related genes have been identified:Mesp1 and Mesp2 in the mouse;Meso-1 and Meso-2 in the chicken;Xl-mespa and Xl-mespb(also known as Thylacine1)in the African clawed frog(Xenopus laevis);and mesp-a and mesp-b in the ***,the functional differences between two Mesp-related genes remain *** the present study,we carried out comparative analyses of the Xl-mespa and Xl-mespb *** amino acid sequences of the Xl-mespa and Xl-mespb proteins showed a high level of *** expression of Xl-mespa started broadly in the ventrolateral mesoderm and gradually shifted to a striped pattern of *** contrast,Xl-mespb showed a striped pattern of expression from the *** expression profiles completely overlapped at the PSM during *** investigate the functional differ-ences between Xl-mespa and Xl-mespb in terms of target gene regulation,we carried out a luciferase assay using the murine Lunatic fringe(L-fng)*** of the L-fng promoter was activated more strongly by Xl-mespb than by *** same pattern was observed for the murine Mesp-related *** results suggest that the functional differences between the two types of Mesp-related genes are evolutionally conserved in vertebrates.

关键词： homology presomitic mesoderm somite segmentation somitogenesis Xenopus laevis.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Mechanisms Contributing to Resistance to Tyrosine Kinase-Targeted Therapy for Non-Small Cell Lung Cancer

引用

Clinical oncology and cancer researeh 2012年第1期9卷 18-22页

作者： Fariz Nurwidya akiko Murakami Fumiyuki Takahashi Kazuhisa Takahashi Department of Respiratory Medicine Juntendo University School of Medicine

One of the most important pathways in non-small cell lung cancer(NSCLC) is the epidermal growth factor receptor(EGFR) pathway. This pathway affects several crucial processes in tumor development and progression,including tumor cell proliferation,apoptosis regulation,angiogenesis,and metastatic *** EGFR is currently being intensely *** are witnessing the development of a number of potential molecular-inhibiting treatments for application in clinical *** the last decade,the tyrosine kinase(TK) domain of the EGFR was identified in NSCLC patients,and it has responded very well with a dramatic clinical improvement to TK inhibitors such are gefitinib and ***,there were primary and/or secondary resistance to these treatments,as shown by clinical *** molecular biology studies provided some explanations for the drug resistance *** molecular mechanisms of resistance need to be *** in-depth understanding of these targeted-therapy resistance may help us explore new strategies for overcoming or reversing the resistance to these inhibitors for the future of NSCLC treatment.

关键词： carcinoma non-small-cell lung mutation drug resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：