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Carbon-Ion Beams Efficiently Induce Cell Killing in X-Ray Resistant Human Squamous Tongue Cancer Cells

International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2014年第3期3卷 133-142页

作者： Akihisa Takahashi Hongyu Ma Akiko Nakagawa Yukari Yoshida Tatsuaki Kanai Tatsuya Ohno Yoshikazu Kuwahara Manabu Fukumoto Takashi Nakano Advanced Scientific Research Leaders Development Unit Gunma University Gunma Japan Department of Pathology IDAC Tohoku University Sendai Japan Department of Radiation Oncology Gunma University Graduate School of Medicine Gunma Japan Gunma University Heavy Ion Medical Center Gunma Japan

In order to see whether carbon ion (C-ion) beams have a biological advantage over X-rays, studies were designed to examine the effects of C-ion beams on radiosensitivity in X-ray resistant cells. Clinically relevant X-ray resistant SAS-R cells derived from human tongue cancer SAS cells were used. The cells were exposed to X-rays or Spread-Out Bragg peak (SOBP) beam C-ions. Cell survival was measured using a modified high-density survival assay. Cell survival signaling and cell death signaling were analyzed using flow cytometry. The cells were labeled with putative cancer stem cell markers such as CD44 and CD326. SAS-R cells were 1.6 times more radioresistant than SAS cells after exposure to X-rays. Cell survival was similar in each cell line after exposure to C-ion beams. SAS-R cells displayed enhanced cell survival signaling when compared to SAS cells under normal conditions. On the other hand, the phosphorylation of AKT-related proteins decreased and polycaspase activities were enhanced when cells were irradiated with C-ion beams in both cell lines. More CD44 and CD326 positive cells were seen in SAS-R cells than in SAS cells. Moreover, the marker positive cell numbers significantly decreased after exposure to C-ion beams when compared to X-rays at iso-survival doses in SAS-R cells. C-ion beams efficiently induced cell killing in X-ray resistant cells which displayed activated cell survival signaling and contained more numerous cancer stem-like cells.

关键词： Carbon-Ion Beams X-Ray Resistant Cells Cell Survival and Death Signaling Cancer Stem-Like Cells

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Cognitive Behavioral Therapy as the Basis for Preventive Intervention in a Sleep Health Program: A Quasi-Experimental Study of E-Mail Newsletters to College Students

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Open Journal of Medical Psychology 2015年第1期4卷 9-16页

作者： Kenichi Asano Ikuo Ishimura Hironori Abe Michiko Nakazato Akiko Nakagawa Eiji Shimizu Research Center for Child Mental Development Graduate School of Medicine Chiba University Chiba Japan Department of Clinical Psychology Tokyo Seitoku University Chiba Japan

Several reports suggest that college students often have atypical sleep patterns and experience poor sleep quality. We examined the effect of a cognitive behavioral therapy (CBT) based intervention program to improve sleep quality and overall mental health among college students. The intervention was delivered in the form of e-mail newsletters. Fifty-three students participated in the intervention group, and another 50 students participated in the control group. The intervention group received a lecture on sleep hygiene;once-weekly e-mail newsletters on sleep health topics (sleep hygiene, stimulus control, sleep restriction, sleep titration, and relapse prevention) and were asked to maintain a four-week sleep diary. The Pittsburgh Sleep Quality Index (PSQI), a measure of sleep quality, and the Kessler 6, a measure of psychological distress, were administered before the lecture (as the baseline measurement) and again 16 weeks later (follow-up measurement). PSQI and K6 scores were reduced in the intervention group compared with the control group. A CBT-based sleep health program utilizing e-mail newsletters may work to improve sleep quality and mental health. This program may represent a cost effective way for Japanese students to receive treatment for poor sleep and may also serve to prevent psychiatric problems.

关键词： Sleep College Students Insomnia Mental Health Cognitive Behavioral Therapy

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一氧化氮合成酶、环氧化酶-2、硝基酪氨酸及血管生成与软骨肉瘤预后的相关性研究

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中华骨科杂志 2010年第12期30卷 I0041-I0049页

作者： Suely Akiko Nakagawa Ademar Lopes Andre Lopes de Carvalho Benedito Mauro Rossi Isabela Werneck da Cunha Fernando Augusto Soares Wu Tu Chung Luciola Assuncao 刘沛宜(译) 张伟滨(译) Investigation performed at Departamentos de Cirurgia Petvica-Ortopedia e Anatomia Patologica Hospital A.C. Camargo Sao Paulo Brazil 上海交通大学医学院附属瑞金医院骨科 200025

背景：免疫组织化学标记物有助于肿瘤诊断及预后评估。通过对硝基酪氨酸分析及采用一氧化氮合成酶1（NOS1）、一氧化氮合成酶2（NOS2）、一氧化氮合成酶3（NOS3）检测分析可了解一氧化氮（NO）与肿瘤的相关性。NO与超氧阴离子反应产生... 详细信息

背景：免疫组织化学标记物有助于肿瘤诊断及预后评估。通过对硝基酪氨酸分析及采用一氧化氮合成酶1（NOS1）、一氧化氮合成酶2（NOS2）、一氧化氮合成酶3（NOS3）检测分析可了解一氧化氮（NO）与肿瘤的相关性。NO与超氧阴离子反应产生的过氧亚硝酸对基因有毒性作用，过氧亚硝酸传递一个硝基基团到酪氨酸的苯环上形成硝基酪氨酸。细胞内硝基酪氨酸的积累说明过氧亚硝酸持续生成。NO刺激所生成的环氧化酶-2（COX-2）与肿瘤内新生血管水平相关，研究表明新生血管影响肿瘤预后，免疫组化标记CD34可表示微血管生成，从而被用于预测肿瘤预后。本研究检测上述标记物在软骨肉瘤中的表达并分析与软骨肉瘤分级和预后的相关性。方法：101例软骨肉瘤患者肿瘤组织样本采用甲醛固定，组织样本采用免疫组织化学染色，指标为：NOS1、NOS2、NOS3、COX-2、硝基酪氨酸和CD34。5例正常软骨样本作为对照。研究分析患者特征、手术变量、肿瘤分级与这些指标的相关性。使用Kaplan—Meier多变量统计学方法分析生存曲线及生存率。结果：硝基酪氨酸、COX-2和CD34与组织学分级有明显相关性（p=0．022，p=0．014，p=0．028），但与总体预后无相关性（p=0．064，p=0．143，p=0．581）。NOS2阳性表达常预示较高的局部复发率（p=0．038）。NOS1、NOS2阳性表达预示较低的生存率（p=0．007，p〈0．001）。通过多变量分析处理，NOS2的表达被证实可作为局部复发率的独立预后判断因素；NOS1、NOS2的表达作为因变量，它们单独或复合阳性表达常与较低的总生存率有关（p=0．046，p=0．004）[危险率，3．17（95％置信区间，1．0-9．8）和5．58（95％置信区间，1．7-18．0）]。

关键词：一氧化氮合成酶硝基酪氨酸环氧化酶-2 软骨肉瘤微血管生成预后评估相关性免疫组织化学染色

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