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Genotypic Variation in Spatial Distribution of Fe in Rice Grains in Relation to Phytic Acid Content and Ferritin Gene Expression

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Rice science 2020年第3期27卷 227-236页

作者： Anurag MISHRA MdSHAMIM MdWASIM Siddiqui akanksha singh Deepti SRIVASTAVA K.N.singh Department of Plant Molecular Biology and Genetic Engineering Narendra Deva University of Agriculture and TechnologyKumarganjFaizabadUttar Pradesh 224229India Division of Genetics ICAR-Indian Agricultural Research InstituteNew Delhi 110012India Department of Molecular Biology and Genetic Engineering Dr.Kalam Agricultural CollegeKishanganj(Bihar Agricultural UniversitySabourBhagalpur)Bihar 855107India Department of Post-Harvest Technology Bihar Agricultural UniversitySabourBhagalpurBihar 813210India Integral Institute of Agricultural Science and Technology(IIAST)Integral University Kursi RoadDasauliUttar Pradesh 226026India

Rice varieties having high Fe concentration in the endospermic region can be used as a good source for Fe deficit *** this study,303 Oryza sativa varieties and 1 Oryza rufipogon accession were assessed for spatial Fe accumulation in grains by Prussian blue staining *** ferritin protein distribution in grains was visualized by immunohistochemistry,and ferritin expression was assessed in selected rice varieties using semi-quantitative reverse transcription *** popular rice varieties,namely Sarjoo 52,Madhukar and Jalmagna,and the *** variety showed Fe in all the regions of grains,and the highest Fe concentration was observed in the embryo *** high-yielding varieties like Swarna,Swarna Sub 1,CSR13 and NDRR359 had lower Fe concentration in the embryo *** highest Fe concentration was detected in ***(49.8μg/g),followed by Sarjoo 52(26.1μg/g)and Madhukar(25.7μg/g).Phytic acid concentration was the minimum in ***(5.75 mg/g)followed by Sarjoo 52(5.83 mg/g).Western blot and semi-quantitative reverse transcription PCR showed higher expression of ferritin gene in ***,Sarjoo 52 and *** conclusion,*** and Sarjoo 52 had higher Fe concentration in the embryo regions as well as endosperm and aleurone layer,whereas the other varieties had lower Fe concentration in the *** 52 could be used as a donor in the rice breeding program for the generation of new varieties with elevated grain Fe concentration.

关键词： bio-fortification iron accumulation ferritin tissue-specific localization iron deficit population

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Herbal tea formulation using different flavoured herbs with dried corn silk powder and its sensory and phytochemical analysis

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Systems Microbiology and Biomanufacturing 2021年第3期1卷 336-343页

作者： akanksha singh Rita singh Raghuvanshi Amit Bhatnagar Department of Foods and Nutrition College of Home ScienceG.B.Pant University of Agriculture and TechnologyPantnagar 263145UttarakhandIndia Department of Agronomy College of AgricultureG.B.Pant University of Agriculture and TechnologyPantnagar 263145UttarakhandIndia

The aim of this study was to use dried corn silk powder variety of VL Baby corn-1 for formulation of flavoured herbal tea with different combination of Thyme(Trachyspermum ammi),Clove(Syzygium aromaticum),Cinnamon powder(Cinnamomum verum),Timur(Zanthoxylum alatum),Tulsi(Holy basil)(Ocimum tenuiflorum),Gandraini(Achillea millefolium),Lemon grass(Cymbopogon citratus),Blend(Clove+Timur+Gandraini),and one only corn silk without any other herbs and to know antioxidant properties of developed *** of sensory evaluation experiment showed that all combinations were liked *** grass flavour followed by cinnamon and clove blend obtained highest *** phenolic content,total flavonoid content,DPPH free radical scavenging activity and total antioxidant activity for all flavour are analyzed and *** on DPPH free radical scavenging activity indicated that corn silk tea had 81.215±0.75μg/mL whereas blend with Timur had significant highest value and Thyme blend had lowest value 64.20±0.40 among all eight different blends with corn *** values for total antioxidant capacity blends with Timur(Zanthoxylum alatu)in corn silk showed significantly(P<0.05)highest value and Clove,Timur,Gandraini blends got lowest value.

关键词： Herbal tea Corn silk Sensory parameters Antioxidants Phenolic content

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Expression of TTV-ORF2 Protein for Detection of Anti-TTV IgG Antibodies in Human Sera

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Advances in Infectious Diseases 2013年第3期3卷 223-229页

作者： Shiwani singh akanksha singh Dhananjay singh Mankotia Kalpana Luthra Mohammad Irshad Clinical Biochemistry Division Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi India

The present study describes the cloning and expression of ORF-2 region of TTV genome and the use of expressed peptide in developing immunoassay for detection of anti-TTV antibodies in serum. Presence of TTV-DNA in serum was detected by PCR amplifying N-22 region of ORF-1 of TTV genome. This was followed by genotyping of TTV by RFLP using N-22 amplicon. Using genotype-1 positive serum as the source of TTV, the ORF-2 region was amplified by PCR and subsequently cloned and expressed in pET-19b vector. The expressed protein, identified as 20 kDa protein on SDS-PAGE gel, was purified by affinity chromatography and then used as antigen to develop western blot assay for detection of anti-TTV antibodies in serum. Analysis of sera for anti-TTV antibodies and their comparison with presence of TTV-DNA, produced encouraging results. There was a good relation between presence of anti-TTV and TTV-DNA in these sera samples. Anti-TTV antibodies could be detected in all TTV-DNA positive sera irrespective of the presence of TTV-genotype. This investigation demonstrates that ORF-2 peptide may be used in developing immunoassay for identification of TTV infection.

关键词： TTV ORF-2 Anti TTV-IgG Expression N-22

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Excipient effect on antimicrobial activity of Cinnamon (Cinnamomum zelylanicum album) oil, Thyme (Thymus vulgaris) oil and Ajowan (Trachyspermum ammi) oil

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Infectious Diseases Research 2024年第1期5卷 27-31页

作者： Bhoj R singh Shiv Varan singh Ravikant Agarwal akanksha Yadav Division of Epidemiology ICAR-Indian Veterinary Research InstituteBareilly 243122India Division of Bacteriology and Mycology ICAR-Indian Veterinary Research InstituteBareilly 243122India Division of Livestock Products Technology ICAR-Indian Veterinary Research InstituteBareilly 243122India.

Background:Many herbal essential oils are potential antimicrobials but their pharmaceutical utility is restricted due to a lack of suitable excipients to mollify their dermatotoxicity and irritant property,and outcome of their therapeutic use may vary with different diluents ***:Effect of 16 diluents(dimethyl sulfoxide,liquid paraffin,glycerine,oils of mustard,sunflower,rice bran,palm,groundnut,olive,coconut,sesame,avocado,jojoba,castor,linseed and soybean)was assessed on antimicrobial activity of 2%cinnamon(Cinnamomum zelylanicum album),thyme(Thymus vulgaris)and ajowan(Trachyspermum ammi)oils using agar well diffusion *** effect of excipients was evaluated on six Candida albicans,five Escherichia coli,four Acinetobacter lwoffii,two strains each of Staphylococcus aureus,Enterobacter agglomerans,and Enterococcus faecium and one strain each of Acinetobacter calcoaceticus,Escherichia fergusonii,Klebsiella oxytoca,*** ***,Leclercia adecarboxylata,Paenibacillus amylolyticus,Proteus mirabilis,***,Pseudomonas aeruginosa,Raoultella terrigena,Staphylococcus capitis ***,***,***,*** and Streptococcus ***:Thyme oil(2%)maintained it antimicrobial activity on dilution in dimethyl sulfoxide and glycerine,and ajowan oil(2%)completely lost its antibacterial activity in all diluents except dimethyl ***,cinnamon oil partially lost its antimicrobial activity upon dilution in glycerine,vegetable,and mineral oils in comparison to dimethyl *** oil was the best vegetable oil,almost comparable to dimethyl sulfoxide and castor oil was the worst diluent for maintaining antimicrobial activity of cinnamon ***:The study indicated the non-suitability of vegetable oils for pharmaceutical formulations of essential oils except olive oil for dilution of cinnamon oil and glycerol for thyme oil to replace dimethyl sulfoxide as diluent.

关键词： essential oils herbal antimicrobials herbal drug-resistance Bacteria Candida

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Comparative antimicrobial activity in Murrah buffalo (Bubalus bubalis) Heifers’ urine and urine distillate

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Infectious Diseases Research 2024年第1期5卷 32-39页

作者： Bhoj R singh Himani Agri Dayanath Balusamy akanksha Yadav Varsha Jayakumar Division of Epidemiology ICAR-Indian Veterinary Research InstituteBareilly 243122India.

Background:Murrah buffalo is a breed of water buffalo(Bubalus bubalis)reared for milk and meat,especially in Northern *** are so many studies on the antimicrobial potential of the cow(Bos indicus)urine but buffalo urine has rarely been *** study was aimed to evaluate the antimicrobial activity in whole buffalo urine and its ***:Urine specimens were collected from seven Murrah buffalo heifers in the morning over three days from all seven heifers and distillated to prepare urine distillate(UD).The antimicrobial activity was determined through 96 well microplate method diluting(1:1)urine and UD in Mueller Hinton liquid medium against 919 microbial strains belonging to 148 species of 49 genera(Candia,11 genera of Gram+ve bacteria,37 genera of Gram-ve bacteria).The test strains were taken from the repository,revived and tested for purity before *** test strains were of clinical origin(372),environmental origin(496)and also from reference strain repositories(51).Results:Of the 919 test strains,a total of 57.89%and 56.04%of the tested strains were susceptible to UD and urine,*** was a no significant(p>0.4)difference in the antimicrobial activity of UD and urine against 919 strains of microbes,regardless of their genus,species,and Gram staining ***,there was no significant(p>0.4)difference between the susceptibility of bacterial and Candida strains to UD or ***,strains of clinical origin were more often resistant to buffalo UD and urine(p0.05).A significantly(p<0.01)higher proportion of reference strains was susceptible to UD and urine than microbial strains of clinical and environmental *** buffalo urine and UD,the most susceptible strains were Stenotrophomonas maltophilia(13/13)followed by Citrobacter freundii(8/10);Salmonella enterica ***(21/27),Geobacillus stearothermo

关键词： Bubalus bubalis Edwardsiella tarda Entereococcus faecium Escherichia coli Klebsiella pneumoniae Raoultella terrigena Salmonella enteric

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屏风住宅印度勒克瑙私宅

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室内设计与装修 2020年第7期 80-83页

作者：麦子(编译) 无 Nina Puri Sanjay Puri Ruchika Gupta Jinal Jain Nivedita Raverkar Nandita Rebello akanksha singh Swati Gadekar Dinesh Mehta(摄影) 不详 Nina&Sanjay Puri Architects

印度勒克瑙城拥有丰富的历史文化遗产,许多建筑都可追溯到18世纪。本文介绍的这幢住宅位于该市繁忙的主干道边,是一片私人住宅地块的边缘部分。项目采用了印度传统四合院的建筑形制,以回应城市特征和炎热的气候条件。业主希望在这个两... 详细信息

印度勒克瑙城拥有丰富的历史文化遗产,许多建筑都可追溯到18世纪。本文介绍的这幢住宅位于该市繁忙的主干道边,是一片私人住宅地块的边缘部分。项目采用了印度传统四合院的建筑形制,以回应城市特征和炎热的气候条件。业主希望在这个两层高的通风庭院中穿插设置一些有遮挡的开放式露台和景观花园。同时,除了6间卧室之外,还需要普伽室、书房、影视室以及多功能区域等,并希望这些空间均能通往露台和景观花园。

关键词：私人住宅建筑形制露台历史文化遗产传统四合院屏风边缘部分勒克瑙

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Update on the preclinical and clinical assessment of Withania somnifera: from ancient Rasayana to modern perspectives

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Traditional Medicine Research 2022年第5期7卷 50-64页

作者： akanksha Rashmi singh Atiqul Islam Rimpi Arora Harjeet singh Alok Sharma Department of Pharmacognosy ISF College of PharmacyMogaPunjab 142001India Department of Pharmacology ISF College of PharmacyMogaPunjab 142001India CCRAS Ministry of AYUSHJhansiUttar Pradesh 212658India

The Rasayana plant Withania somnifera(***)Dunal,also known as“Ashwagandha”,has been mentioned in various classical Ayurvedic texts,such as Charaka Samhita,Sushruta Samhita,and *** Ayurvedic drug has been referred to as a tonic that renews the body,provides physical and mental vigor in weakened states,and promotes endurance and *** possesses notable biological activity in many ailments,such as diabetes,conjunctivitis,insomnia,senile dementia,Parkinson’s disease,nervous system disorders,rheumatism,and *** pharmacological activities are due to the presence of diverse active components and their *** lead compounds are found to be effective against anxiety and other central nervous system *** has been proven to be effective and safe for a wide range of ailments from ancient to modern *** reported properties represent the traditional use of *** as indicated in the literature;furthermore,*** is one of the most important prescribed drugs in Ayurveda for its multimodal *** current review highlights the bioactive present and provides an overview of the toxicological and pharmacological studies on ***,including preclinical and clinical *** its earliest utilization to its current application,*** has been recognized to be effective at clinical levels for human health and *** attention to the safety and efficacy of *** would provide more scientific evidence,promoting global acceptance of the Ayurvedic plant.

关键词： Withania somnifera Ayurveda Rasayana pharmacological profile preclinical study clinical study

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Revisiting the role of mesenchymal stem cells in tuberculosis and other infectious diseases

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Cellular & Molecular Immunology 2023年第6期20卷 600-612页

作者： Annu Devi Isha Pahuja Shashi Prakash singh akanksha Verma Debapriya Bhattacharya Ashima Bhaskar Ved Prakash Dwivedi Gobardhan Das Special Centre for Molecular Medicine Jawaharlal Nehru UniversityNew DelhiIndia Immunobiology Group International Centre for Genetic Engineering and Biotechnology New DelhiIndia Department of Molecular Medicine Jamia Hamdard UniversityNew DelhiIndia Centre for Biotechnology Siksha O Anusandhan UniversityBhubneshwarOdishaIndia

Mesenchymal stem cells(MsCs)play diverse roles ranging from regeneration and wound healing to immune *** investigations have indicated the crucial role of these multipotent stem cells in regulating various aspects of the immune *** express unique signaling molecules and secrete various soluble factors that play critical roles in modulating and shaping immune responses,and in some other cases,MSCs can also exert direct antimicrobial effects,thereby helping with the eradication of invading ***,it has been demonstrated that MSCs are recruited at the periphery of the granuloma containing Mycobacterium tuberculosis and exert"Janus"-like functions by harboring pathogens and mediating host protective immune *** leads to the establishment of a dynamic balance between the host and the *** function through various immunomodulatory factors such as nitric oxide(NO),IDO,and immunosuppressive ***,our group has shown that *** uses MSCs as a niche to evade host protective immune surveillance mechanisms and establish *** also express a large number of ABC efflux pumps;therefore,dormant *** residing in MSCs are exposed to a suboptimal dose of ***,it is highly likely that drug resistance is coupled with dormancy and originates within *** this review,we discussed various immunomodulatory properties of MsCs,their interactions with important immune cells,and soluble *** also discussed the possible roles of MsCs in the outcome of multiple infections and in shaping the immune system,which may provide insight into therapeutic approaches using these cells in different infection models.

关键词： Mesenchymal Stem Cells(MsCs) Macrophages Tuberculosis Infectious Diseases T cells Immunomodulation Immunotherapy.

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Cyanobacteria,Lyngbya aestuarii and Aphanothece bullosa as antifungal and antileishmanial drug resources

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Asian Pacific Journal of Tropical Biomedicine 2013年第6期3卷 458-463页

作者： Maheep Kumar Manoj Kumar Tripathi akanksha Srivastava Jalaj Kumar Gour Rakesh Kumar singh Ragini Tilak Ravi Kumar Asthana Department of Botany Faculty of ScienceBanaras Hindu University Department of Biochemistry Faculty of ScienceBanaras Hindu University Department of Microbiology Institute of Medical SciencesBanaras Hindu University

Objective:To investigate two cyanobacteria isolated from different origins *** aestuarii(***)from brackish water and Aphanothece bullosa(***)from fresh water paddy fields for antifungal and antileishmanila activity taking Candida albicans and Leishmania donovain as ***:Biomass of *** and *** were harvested after 40 and60 d respectively and lyophilized twice in methanol(100%)and redissolved in methanol(5%)for *** bioassay was done by agar well diffusion method while antileishmanial,by counting cell numbers and flageller motility observation of promastigotes and amastigotes from *** and 5%methanol were used as ***:Both the cyanobacteria were found to be potent source of antifungal activity keeping fluconazole as positive control,however,methanolic crude extract(15 mg/mL)of *** was found more potent(larger inhibition zone)over that of methanolic crude extract of ***,Similarly antileishmanial activity of crude extract(24.0 mg/mL)of *** was superior over that of methanolic crude extract of ***(25.6 mg/mL).Conclusions:Antifungal and antileishmanial drugs are still limited in the *** of microbes possessing antifungal and antileishmanial activity drug is of prime *** are little explored in this context because most of the drugs in human therapy are derived from microorganisms,mainly bacterial,fungal and *** in the present study two cyanobacterial strains from different origins showed potent source of antifungal and antileishmanial biomolecules.

关键词： Antifungal Antileishmanial Secondary metabolite Cyanobacteria

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Artefacts in cone beam CT

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Open Journal of Stomatology 2013年第5期3卷 292-297页

作者： Prashant P. Jaju Mayuri Jain Ajita singh akanksha Gupta Rishi Raj College of Dental Sciences and Research Centre Bhopal India

Cone beam computed tomography (CBCT) is the modern third dimension applied in the field of oral maxillofacial region. With lower radiation dose compared to conventional CT, its applications in dentistry has increased tremendously. Artefacts can seriously degrade the quality of computed tomographic (CBCT) images, sometimes to the point of making them diagnostically unusable. To optimize image quality, it is necessary to understand why artifacts occur and how they can be prevented or suppressed. CT artifacts originate from a range of sources;physical based, scanner based and patient based. This article highlights the causes of artefacts on CBCT images and methods to avoid them.

关键词： CBCT Artefacts Metal Artefacts

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