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不同水曲柳褐斑病病级叶片的微生物多样性

森林工程 2024年第1期40卷 1-8页

作者：刘思远申东晨刘峥鲁丽颖徐恒董爱荣东北林业大学林学院哈尔滨150040

为探究染褐斑病不同程度的水曲柳叶片微生物多样性和主要致病微生物,分析防治方法以维护水曲柳人工林生态安全、提升其经济效益,在帽儿山实验林场采集染褐斑病不同程度的叶片,采用高通量测序技术,测定并分析水曲柳叶片微生物群落结构和... 详细信息

为探究染褐斑病不同程度的水曲柳叶片微生物多样性和主要致病微生物,分析防治方法以维护水曲柳人工林生态安全、提升其经济效益,在帽儿山实验林场采集染褐斑病不同程度的叶片,采用高通量测序技术,测定并分析水曲柳叶片微生物群落结构和物种组成。3组样品感病前后共得到1 026个真菌分类操作单元(Operational Taxonomic Unit,OUT)及322个细菌OUT。在细菌中,变形菌门和放线菌门为优势菌门;鞘氨醇单胞菌属、醋酸杆菌属、短根瘤菌属等为主要优势属。在真菌中,担子菌门和子囊菌门为优势菌门;维希尼克氏酵母属、壳针孢属、汉纳酵母属为优势菌属。3组样品细菌多样性及群落结构无明显差异,真菌多样性及群落结构差异性显著,初步推测尾孢菌属为病原菌,但其致病性有待进一步研究。

关键词：水曲柳褐斑病高通量测序尾孢菌属菌群结构

Metagenomic insights into seasonal variations in the soil microbial community and function in a Larix gmelinii forest of Mohe,China

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Journal of Forestry Research 2021年第1期32卷 371-383页

作者： Tong Bao Shilin Deng Kaiyue Yu Weiyi Li airong dong Department of Forest Protection College of ForestryNortheast Forestry UniversityHarbin 150040People’s Republic of China

The eff ect of seasons on the soil microbiome in a Larix gmelinii forest of Mohe,China,where winter temperatures are generally below−40°C,was evaluated with metagenomics *** profi ling using sequencing information revealed that Proteobacteria,Actinobacteria,Acidobacteria and Verrucomicrobia were the dominant phyla in spring,summer,and fall,as were Bradyrhizobium,Chthoniobacter,Streptomyces,Acid Candidatus Koribacter at the genus *** species that were abundant in spring and fall greatly diminished in abundance in *** of orthologous groups(COG)of proteins,carbohydrate-active enzymes(CAZy),Kyoto Encyclopedia of Genes and Genomes(KEGG)and NCBI databases were used to elucidate the function of diverse proteins and metabolites of the microbial community of *** *** analysis showed that fewer genes were detected in spring than in fall and summer,indicating that many soil microbes in the *** forest were not tolerant to *** on KEGG analysis,some pathways in the soil microbes were activated in spring and autumn and deactivated in *** analysis revealed that most CAZy were more active in summer than in spring or autumn and were severely inhibited in the *** functional pathways,proteins,and CAZy involved in the community changes were concerned with cold or heat ***,the soil in the *** forest can be a valuable resource for further research on heat and cold tolerance of soil microbes.

关键词： Soil microbial community Microbial function Metagenomic Seasonal variation

Atomically precise Ru-O-Ru clusters for enhanced water dissociation in alkaline hydrogen evolution

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Nano Research 2024年第8期17卷 6993-7000页

作者： dong Liu Li Xu Sicheng Li airong Xu Yuanhua Sun Tong Liu Mengyuan Liu Huijuan Wang Xiaokang Liu Tao Yao Tao Ding Hefei National Research Center for Physical Sciences at the Microscale School of Nuclear Science and TechnologyKey Laboratory of Precision and Intelligent ChemistryNational Synchrotron Radiation LaboratoryUniversity of Science and Technology of ChinaHefei 230029China Experimental Center of Engineering and Materials Science University of Science and Technology of ChinaHefei 230026China

Atomic clusters typically exhibit distinctive electronic structures and physicochemical ***,as the size decreases,their ability to adsorb and dissociate water also diminishes,thereby affecting chemical reactions involving water *** the adsorption and dissociation capabilities of atomic clusters towards water molecules and elucidating the mechanisms underlying their performance enhancement have become important research ***,employing the carrier-anchored strategy,Ru-O-Ru atomic clusters were prepared and displayed excellent activity and durability in the hydrogen evolution ***,the Ru-O-Ru atomic clusters exhibited only 86 mV overpotential at 100 mA·cm−^(2) and superior membrane-electrode-assembly activity than commercial Ru/C *** radiation-based Fourier transform infrared spectroscopic measurements revealed that the modification of oxygen in Ru-O-Ru units promoted the reorientation of water molecules from a H-up orientation to H-down,therefore,enhanced the formation of strong hydrogen-bond network of interfacial water on the surface of Ru-O-Ru clusters,leading to enhanced adsorption and dissociation of water and accelerated Volmer *** findings provide a potential strategy and deep insights for the development of atomic clusters in electrocatalysts.

关键词： hydrogen evolution in-situ Fourier transform infrared(FTIR) Ru catalysts electrocatalysis atomic clusters

Regulating atomic Fe-Rh site distance for efficient oxygen reduction reaction

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Science China Chemistry 2024年第4期67卷 1352-1359页

作者： Tong Liu Yudan Chen airong Xu Xiaokang Liu dong Liu Sicheng Li Hui Huang Li Xu Shuaiwei Jiang Qiquan Luo Tao Ding Tao Yao National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China Key Laboratory of Precision and Intelligent Chemistry Hefei National Research Center for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230026China Institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China

Exploring the atomic interaction mechanisms of dense single-atom catalysts(SACs)is of great significance for their application in oxygen reduction reaction(ORR).However,the intrinsic mechanism of the site-distance effect on the catalytic performance has been largely ***,we demonstrate the site-distance effect of Fe-Rh_(x)@NC catalysts in ORR theoretically and *** charge analysis reveals that the strong interaction between Fe and Rh atoms at a certain atomic distance(dFe-Rh)alters the catalytic electronic structure,facilitating the optimization of catalyst adsorption *** by the theoretical calculations,we designed and synthesized the Fe-Rhx@NC catalysts through a spatial confinement *** characterization results prove that the Fe-Rh_(2)@NC has the optimal d_(Fe-Rh),which improves its intrinsic ORR activity,providing a half wave potential of 0.91 V,higher than that of the commercial Pt/C(0.86 V).This study emphasizes the importance of determining the basic mechanism of the site-distance effect in dissimilar metal atoms catalysts,which is conducive to the design of efficient catalyst systems for practical applications.

关键词： Density functional theory single-atom catalyst in-situ encapsulating site-distance effect oxygen reduction reaction

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红豆杉健康与感叶枯病针叶内生微生物多样性

森林工程 2023年第4期39卷 10-18页

作者：王俊凯刘峥申东晨董爱荣东北林业大学林学院哈尔滨150040

采用Illumina Miseq高通量测序技术来探究人工林与天然林东北红豆杉(Taxus cuspidata)健康针叶与感叶枯病的病叶之间内生真菌和细菌群落结构的差异。结果表明,红豆杉针叶感病前后真菌的OTU(Operational Taxonomic Units)变化较小,细菌的... 详细信息

采用Illumina Miseq高通量测序技术来探究人工林与天然林东北红豆杉(Taxus cuspidata)健康针叶与感叶枯病的病叶之间内生真菌和细菌群落结构的差异。结果表明,红豆杉针叶感病前后真菌的OTU(Operational Taxonomic Units)变化较小,细菌的OTU变化较大,天然林比人工林具有更多的真菌OTU数,细菌OTU数差距较小。4组样品中共有相同的真菌OTU 9个,占真菌OTU总数的9.37%,4组样品中共有相同的细菌OTU 34个,占细菌OTU总数的4.19%。在门水平下,感病前后样品中的真菌群落结构组成基本相同,差异较大的是相对多度,相比于天然林,子囊菌门(Ascomycota)在人工林中的相对多度更高,子囊菌门在4组样品占据绝对优势。感病前后样品中的细菌群落结构组成及相对多度都发生了较大变化,相比于天然林,人工林中细菌种类更多。在属水平下,Genolevuria属是人工林健康叶中最主要的优势真菌,穴壳属(Dothiora)是人工林病叶中最主要的优势真菌,Buckleyzyma属是天然林健康叶已知属中最主要的优势真菌,Genolevuria属是天然林病叶已知属中最主要的优势真菌。劳尔氏菌属(Ralstonia)是人工林健康叶中最主要的优势细菌,1174-901-12属是人工林病叶中最主要的优势细菌,鞘氨醇单胞菌属(Sphingomonas)是天然林健康叶已知属中最主要的优势细菌,马赛菌属(Massilia)是天然林病叶已知属中最主要的优势细菌。推测出链格孢属(Alternaria)和盘双端毛孢属(Seimatosporium)可能会导致红豆杉的叶枯病。

关键词：高通量测序红豆杉叶枯病真菌多样性细菌多样性

产低温脂肪酶菌株鉴定、发酵条件优化及酶学性质分析

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食品工业科技 2023年第20期44卷 116-125页

作者：刘思远申东晨刘峥鲁丽颖徐恒董爱荣东北林业大学林学院黑龙江哈尔滨150040

为筛选高产低温脂肪酶的菌株,并对产酶条件进行优化,同时为脂肪酶的工业化开发提供生产资料。从黑龙江省漠河县土壤样品中筛选出一株产低温脂肪酶菌株,通过形态学鉴定、生理生化实验及分子生物学鉴定,确定该菌株为普城沙雷氏菌(Serratia... 详细信息

为筛选高产低温脂肪酶的菌株,并对产酶条件进行优化,同时为脂肪酶的工业化开发提供生产资料。从黑龙江省漠河县土壤样品中筛选出一株产低温脂肪酶菌株,通过形态学鉴定、生理生化实验及分子生物学鉴定,确定该菌株为普城沙雷氏菌(Serratiaplymuthica)。通过单因素实验,探究温度、pH、装液量、接种量、碳源、氮源、金属离子、诱导剂等不同因素对菌株产酶的影响,通过Plackett-Burman实验,爬坡试验及响应曲面设计,优化橄榄油、蛋白胨、装液量等因素的添加量。结果表明:该菌株最优产酶条件为20℃、pH7.5、装液量42 mL、接种量0.5%、20 g/L麦芽糖、14 g/L蛋白胨、0.5 g/L的MgSO_(4)·7H_(2)O及46 mL/L橄榄油。此优化条件下,脂肪酶活为98.05 U/mL,是优化前的5.85倍。酶学性质结果表明,该脂肪酶最适温度为30℃,属于低温脂肪酶,最适反应pH为7,Mg^(2+)明显可以促进酶活。有机溶剂中甲醇和乙醇明显抑制了酶活性,而正己烷明显可以促进酶活性。该结论可为微生物资源开发利用,工业生产低温脂肪酶提供一定的理论依据及方法指导。

关键词：低温脂肪酶普城沙雷氏菌发酵条件优化酶学性质

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Resonance mechanism of flapping wing based on fluid structure interaction simulation

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Chinese Journal of Aeronautics 2024年第5期37卷 243-262页

作者： Yueyang GUO Wenqing YANG Yuanbo dong dong XUE National Key Laboratory of Science and Technology on Aerodynamic Design and Research School of AeronauticsNorthwestern Polytechnical UniversityXi'an 710072China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518063China Yangtze River Delta Research Institute of Northwestern Polytechnical University Taicang 215400China

Certain insect species have been observed to exploit the resonance mechanism of their *** order to achieve resonance and optimize aerodynamic performance,the conventional approach is to set the flapping frequency of flexible wings based on the Traditional Structural Modal(TSM)***,there exists controversy among researchers regarding the relationship between frequency and aerodynamic *** that the structural response of wings can be influenced by the surrounding air vibrations,an analysis known as Acoustic Structure Interaction Modal(ASIM)is introduced to calculate the resonant *** this study,Fluid Structure Interaction(FSI)simulations are employed to investigate the aerodynamic performance of flapping wings at modal frequencies derived from both TSM and ASIM *** performance is evaluated for various mass ratios and frequency ratios,and the findings indicate that the deformation and changes in vortex structure exhibit similarities at mass ratios that yield the highest aerodynamic ***,the flapping frequency associated with the maximum time-averaged vertical force coefficient at each mass ratio closely aligns with the ASIM frequency,as does the frequency corresponding to maximum ***,the ASIM analysis can provide an effective means for predicting the optimal flapping frequency for flexible ***,it enables the prediction that flexible wings with varying mass ratios will exhibit similar deformation and vortex structure *** paper offers a fresh perspective on the ongoing debate concerning the resonance mechanism of Flexible Flapping Wings(FFWs)and proposes an effective methodology for predicting their aerodynamic performance.

关键词： Flexible Flapping Wing(FFW) Acoustic Structure Interaction Modal(ASIM) Fluid Structure Interaction(FSI) Resonance mechanism Aerodynamic performance

大兴安岭优势树种倒木分解的细菌群落组成及多样性差异

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中南林业科技大学学报 2023年第9期43卷 105-115页

作者：周宇潘虹杜君杨立宾董爱荣东北林业大学林学院黑龙江哈尔滨150040 黑龙江省科学院自然与生态研究所黑龙江哈尔滨150040 黑龙江呼中国家级自然保护区管理局黑龙江大兴安岭地区165100

【目的】探究大兴安岭林地不同优势树种倒木分解过程中细菌群落组成、多样性及功能变化,探讨养分驱动下主要细菌群落分布格局的差异化响应。【方法】以一年期的白桦、樟子松、落叶松倒木为研究对象,测定其含水率(MC)、p H值、全碳(TC)... 详细信息

【目的】探究大兴安岭林地不同优势树种倒木分解过程中细菌群落组成、多样性及功能变化,探讨养分驱动下主要细菌群落分布格局的差异化响应。【方法】以一年期的白桦、樟子松、落叶松倒木为研究对象,测定其含水率(MC)、p H值、全碳(TC)、全氮(TN)、纤维素、半纤维素及木质素含量;采用Illumina MiSeq高通量测序技术分析细菌组成、相对丰度和生态功能群组;通过相关性分析探讨不同树种的细菌群落组成与倒木养分间的关联性。【结果】3种倒木分解过程中细菌种类由多到少依次为白桦、落叶松、樟子松,其中白桦独有细菌数量显著多于落叶松、樟子松。群落组成及分布研究表明,变形菌门Proteobacteria为3种倒木中细菌群落的绝对优势菌门,组内相对丰度在不同树种分解倒木中呈显著差异;Lysinimonas、Sphingomonas、Luteibacter为优势菌属,组内相对丰度在不同倒木中无显著差异。群落多样性差异分析表明,树种差异显著改变了樟子松与落叶松、樟子松与白桦细菌群落组内的Alpha多样性,在门、属水平上显著改变了组间的Beta多样性。倒木养分及与群落的相关性分析表明,TC、pH值、TN、C/N、半纤维素、木质素在3种倒木间均呈显著差异,其中TC、pH值、TN、C/N、纤维素、木质素为门水平上,pH值、TN、C/N、纤维素为属水平上影响倒木细菌组成及分布的关键因子。细菌功能预测结果表明,碳水化合物代谢、氨基酸代谢等方式是倒木细菌参与的主要功能通路,但均未表现出树种特异性。【结论】倒木分解过程中不同优势树种内细菌群落组成体现出明显的差异性,但菌群的主要功能通路未表现树种特异性。

关键词：大兴安岭倒木分解细菌多样性高通量测序

Copper slag assisted coke reduction of phosphogypsum for sulphur dioxide preparation

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Chinese Journal of Chemical Engineering 2024年第1期65卷 43-53页

作者： dong Ma Qinhui Wang State Key Laboratory of Clean Energy Utilization Institute for Thermal Power EngineeringZhejiang UniversityHangzhou 310027China

The reduction of phosphogypsum(PG)to lime slag and SO_(2)using coke can effectively alleviate the environmental problems caused by ***,the PG decomposition temperature remains high and the product yield remains *** adding additives,the decomposition temperature can be further reduced and PG decomposition rate and product yield can be ***,the use of current additives such as Fe_(2)O_(3)and SiO_(2)brings the problem of increasing economic ***,it is proposed to use solid waste copper slag(CS)as a new additive to reduce PG to prepare SO2,which can reduce the cost and meet the environmental benefits at the same *** effects of proportion,temperature and thermostatic time on PG decomposition are investigated by experimental and kinetic analysis combined with FactSage thermodynamic calculations to optimize the roasting ***,the reaction mechanism is *** is found that adding CS to the coke and PG system can increase the rate of PG decomposition and SO_(2)yield while lowering the PG decomposition *** example,when the CS/PG mass ratio increases from 0 to 1,PG decomposition rate increases from 83.38%to 99.35%,SO_(2)yield increases from 78.62%to 96.81%,and PG decomposition temperature decreases from 992.4℃to 949.6℃.The optimal reaction parameters are CS/PG mass ratio of 1,Coke/PG mass ratio of 0.06 at 1100℃for 20 min with 99.35%PG decomposition rate and 96.81%SO_(2) *** process proceeds according to the following reactions:2CaSO_(4)+ 0.7C + 0.8Fe_(2)SiO_(4)→0.8Ca_(2)SiO_(4)+ 0.2Ca_(2)Fe_(2)O_(5)+ 0.4Fe_(3)O_(4)+2SO_(2)+ 0.7CO_(2)Finally,a process for decomposing PG with coke and CS is proposed.

关键词： Phosphogypsum Sulfur dioxide Copper slag Fluidized-bed Reduction Waste treatment