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Isolation of the promoter of a cotton β-galactosidase gene (GhGal1) and its expression in transgenic tobacco plants

Science China(Life Sciences) 2006年第2期49卷 105-114页

作者： WU aimin liu Jinyuan Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

β-galactosidases (EC 3.***.1.23) constitute a widespread family of glycosyl hydrolases in plants and are thought to be involved in metabolism of cell wall polysaccharides. A cDNA of the cotton (Gossypium hirsutum) β-galactosidase gene, designated GhGal1, has previously been identified and its transcripts are highly abundant at the elongation stage of the cotton fiber. To examine the temporal and spatial control of GhGal1 expression, a transcriptional fusion of the GhGal1 promoter region (1770 bp) with the β-glucuronidase (GUS) reporter gene was introduced into tobacco plants by the Agrobacterium infection method. The resulting transgenic plants showed higher GUS activity of fruit in the transgenic plants than that in the negative and positive controls. Histochemical localization of GUS activity demonstrated that the expression of the GUS gene could be found in the meristem zones of roots, cotyledons, vascular tissues, fruit and trichomes in transgenic tobacco plants. Additionally, se-quence analysis of the regulatory region also revealed several conserved motifs among which some were shared with previously reported fruit/seed-specific elements and the others were related with trichome expression. These results indicated the temporal and spatial expression characterization of the GhGal1 promoter in transgenic tobacco plants and provided an important insight into the roles of GhGal1 in cotton fiber development.

关键词： cotton, β-galactosidase, transgenic tobacco, promoter activity, fruit and trichome expression.

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Hedgehog signaling:mechanisms and evolution

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Frontiers in Biology 2011年第6期6卷 504-521页

作者： Xuan YE aimin liu Department of Biology Eberly College of ScienceThe Pennsylvania State UniversityUniversity ParkPA 16802USA

The Hedgehog(Hh)family of secreted proteins plays essential roles in the development of a wide variety of animal species and underlies multiple human birth defects and *** ensure the proper range of signaling,the Hh proteins are modified with lipids,assembled into water-soluble multimers,and interact with multiple cell surface *** the target cells,a largely conserved intracellular signal transduction pathway,from the cell surface receptor Patched to the Glioma-associated oncogene homolog(Gli)family of transcription factors,mediates the transcriptional responses from fruit flies to mammals.A significant divergence between vertebrates and insects is the vertebrate-specific requirement of cilia for Hh signal transduction and Gli protein ***,transcription-independent cellular responses to Hh have been described in certain developmental *** clinical trial underway to treat Hhrelated diseases,more work is urgently needed to reach a more comprehensive understanding of the molecular mechanisms underlying the regulation of Hh signaling in development and diseases.

关键词： Hedgehog Shh Ihh Dhh Cubitus interruptus(Ci) Gli1 Gli2 Gli3 Patched Smoothened Cilia Drosophila mouse signaling development evolution

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The n-type Si-based materials applied on the front surface of IBC-SHJ solar cells

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Chinese Physics B 2019年第9期28卷 353-358页

作者： Jianhui Bao Ke Tao Yiren Lin Rui Jia aimin liu School of Microelectronics Dalian University of TechnologyDalian 116024China Institute of Microelectronics of Chinese Academy of Sciences Beijing 100029 China School of Physics&Optoelectronic Engineering Dalian University of TechnologyDalian 116024China

Interdigitated back contact silicon hetero-junction(IBC-SHJ) solar cells exhibit excellent performance owing to the IBC and SHJ *** front surface field(FSF) layer composed of electric field passivation and chemical passivation has been proved to play an important role in IBC-SHJ solar *** electric field passivated layer n^+-a-Si: H, an n-type Si alloy with carbon or oxygen in amorphous phase, is simulated in this study to investigate its effect on *** is indicated that the n^+-a-Si: H layer with wider band gap can reduce the light absorption on the front side efficaciously,which hinders the surface recombination of photo-generated carriers and thus contributes to the improvement of the short circuit current density *** highly doped n^+-a-Si: H can result in the remakable energy band bending, which makes it outstanding in the field passivation, while it makes little contribution to the chemical *** is noteworthy that when the electric field intensity exceeds 1.3 × 10^5 V/cm, the efficiency decrease caused by the inferior chemical passivation is only 0.16%.In this study, the IBC-SHJ solar cell with a front n^+-a-Si: H field passivation layer is simulated, which shows the high efficiency of 26% in spite of the inferior chemical passivation on the front surface.

关键词： Si-based doped materials passivation interdigitated back contact silicon hetero-junction(IBC-SHJ) solar cell simulation

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Effect of wind field below unmanned helicopter on droplet deposition distribution of aerial spraying

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International Journal of Agricultural and Biological Engineering 2017年第3期10卷 67-77页

作者： Chen Shengde Yubin Lan Li Jiyu Zhou Zhiyan liu aimin Mao Yuedong College of Engineering South China Agricultural University/Engineering Research Center for Agricultural Aviation Application(ERCAAA)Guangzhou 510642China International Laboratory of Agricultural Aviation Pesticide Spraying Technology Guangzhou 510642China Hunan Longping Seed Industry Co. LtdChangsha 410006China Shenzhen Hi-Tech New Agriculture Technologies Co. LtdShenzhen 518057China

Wind field is one of the important factors affecting the distribution characteristics of aerial spraying droplet *** order to reveal the impact mechanism of droplet deposition distribution by the wind field below agricultural unmanned helicopter rotor,in this study,the wind field distribution below uniaxial single-rotor electric unmanned helicopter rotor was measured by using a wireless wind speed sensor network measurement system for unmanned *** effects of wind field in three directions(X,Y,Z)below the rotor on droplet deposition distribution were analyzed with the condition of aerial spraying droplet deposition in rice canopy,and the regression model was established via variance and regression analyses of experiment *** results showed that,the wind field in Y direction had a significant effect on droplet deposition in effective spray area,the wind field in Z direction had an extremely significant effect on droplet deposition in effective spray area,and the corresponding significance(sig.)values were 0.011 and ***,the wind field in Z direction had a significant effect on the penetrability and uniformity of droplet deposition in effective spray area,the corresponding *** were 0.025 and 0.011 *** wind speed in Y direction at the edge of effective spray area had a significant effect on droplet drift,the *** was *** addition,the correlation coefficient R of the regression model was 0.869 between droplet deposition in effective spray area and the wind speed in Y and Z directions,and 0.915 between the uniformity of droplet deposition in effective spray area and the maximum wind speed in Z *** result revealed the influencing mechanism of the wind field below the rotor of uniaxial single-rotor electric unmanned helicopter on the distribution of aerial spraying droplet *** results can provide guidance for the actual production application of aerial spraying to reduce liquid drift

关键词： uniaxial single-rotor electric unmanned helicopter aerial spraying wind field droplet deposition

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Digital Economy:A New Chapter Opened with the Development of the Industrial Internet

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China News Release 2022年第4期 16-19页

作者： liu aimin 不详

As a new-type infrastructure,application model and industrial ecology featuring an in-depth integration of the new generation of information and communication technologies(ICT)and the industrial economy.

关键词： Economy Open featuring

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Pathologic complete response confirmed by surgical resection for liver metastases of gastrointestinal stromal tumor after treatment with imatinib mesylate

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World Journal of Gastroenterology 2008年第23期14卷 3763-3767页

作者： Seiji Suzuki Koji Sasajima Masayuki Miyamoto Hidehiro Watanabe Tadashi Yokoyama Hiroshi Maruyama Takeshi Matsutani aimin liu Masaru Hosone Shotaro Maeda Takashi Tajiri Department of Surgery Tama-Nagayama Hospital Nippon Medical School 1-7-1 Nagayama Tama Tokyo 206-8512 Japan Department of Pathology Tama-Nagayama Hospital Nippon Medical School 1-7-1 Nagayama Tama Tokyo 206-8512 Japan Department of Surgery Nippon Medical School 1-1-5 Sndagi Bunkyo-Ku Tokyo 113-8603 Japan

A 39-year-old male underwent distal gastrectomy for a high grade gastrointestinal stromal tumor(GIST) . Computed tomography(CT) and magnetic resonance imaging(MRI) 107 mo after the operation,revealed a cystic mass(14 cm in diameter) and a solid mass(9 cm in diameter) in the right and left lobes of the liver,respectively. A biopsy specimen of the solid mass showed a liver metastasis of GIST. The patient received imatinib mesylate(IM) treatment,400 mg/day orally. Following the IM treatment for a period of 35 mo,the patient underwent partial hepatectomy(S4 + S5) . The effect of IM on the metastatic lesions was interpreted as pathologic complete response(CR) . Pathologically verified cases showing therapeutic efficacy of IM have been rarely reported.

关键词： Gastrointestinal stromal tumor Liver metastasis Imatinib mesylate Pathologic complete response

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Effect of Corona Treatment and Dielectric Separation on Seed Vigor of Hybrid Rice

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Agricultural Biotechnology 2018年第4期7卷 48-54页

作者： Jia PANG Hong liu Haiqing ZHANG aimin liu Jiwai He Ye liu Junlong liu Yixin ZHANG College of Agronomy Hunan Agricultural UniversityChangsha 410128China Hunan Longping Seed Co.Ltd. Changsha 410006China

[Objectives] The aim was to investigate the effects of corona field and dielectric separation on the seed vigor of hybrid rice.[Methods] The seeds of 6 hybrid rice varieties were processed with corona field, dielectric separation and corona field ＋ dielectric separation.[Results] When the field intensity was 200 km/m and the processing time was 4 min, the seed vigor was significantly increased. The seed vigor of Y Liangyou 9918 （2014） increased the most, and the germination potential and the germination rate increased by 17 and 12 percentage points respectively compared to the control. Corona field reduced seed conductivity, and for seedlings, it decreased their MDA content, increased proline content, improved peroxidase activity, and enhanced root activity. When the rotating speed of the drum was 30 r/min, the dielectric separation voltage of 4 500 V could achieve good separation effect, and the vitality of seeds at different grades was significantly different. Under the optimized corona field combined with optimized voltage processing of dielectric separation, the seed vigor at all grades from the separation was significantly higher than that of the dielectric separation processing alone. The combined separation processing separated no seeds with germination rate of higher than 80% from the hybrid rice seeds with germination rate of lower than 60%, yet it could effectively separate the seeds with germination rate of higher than 80% from the hybrid rice seeds with germination rate of 65%-70%. After the combined processing, the germination rates of separated Grade I seeds of Wangliangyou 6 （2015）, N Liangyouhuazhan （2015）, Wangliangyou 6 （2016） increased by 20, 15, 20 percentage points from the germination rates of the control, while the germination rates of N Liangyouhuazhan （2016） and Y Liangyou 9918 （2016）, which had the germination rates of higher than 80%, increased by 3 and 9 percentage points from the control, suggesting insignificant separation effect.[Concl

关键词： Corona treatment Dielectric separation Hybrid rice Seed vigor

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从五行历到四时历--三十时的形成、发展和消亡

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文史哲 2021年第5期 108-119,167页

作者：刘爱敏山东师范大学齐鲁文化研究院山东济南250014

《管子·幼官》和银雀山汉简《三十时》皆记载了一种以十二日为一时、一年为三十时的时令,这与传统的二十四节气不同,属于五行历系统,带有鲜明的齐地特色。三十时划分时节的依据是气在一年内的消息变化,其测时方法主要有以律候气、考察... 详细信息

《管子·幼官》和银雀山汉简《三十时》皆记载了一种以十二日为一时、一年为三十时的时令,这与传统的二十四节气不同,属于五行历系统,带有鲜明的齐地特色。三十时划分时节的依据是气在一年内的消息变化,其测时方法主要有以律候气、考察星象和测量日影,纪时方法是用干支纪日。在战国时期四时原则被普遍遵循的背景下,三十时呈现出由五行历向四时历转变的发展趋势。在汉初太初历颁布后,三十时因与农事节律不一致,以及与全年时长、十二个月等多方面的矛盾无法调和,最终被二十四节气所取代。

关键词：三十时五行历四时历二十四节气

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银雀山汉简《迎四时》与周秦之际的历法整合

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孔子研究 2018年第6期 72-79页

作者：刘爱敏山东师范大学齐鲁文化研究院

银雀山汉简有《迎四时》一篇,所记为天子迎春夏秋冬四时之乐,与《汉书·郊祀志》所记《青阳》《朱明》《西皞》和《玄冥》四首乐歌应属配套使用的乐曲。其乐名为"邹子乐",当系邹衍创作,表达的思想主旨是阴阳五行家的四时教令思想。《迎... 详细信息

银雀山汉简有《迎四时》一篇,所记为天子迎春夏秋冬四时之乐,与《汉书·郊祀志》所记《青阳》《朱明》《西皞》和《玄冥》四首乐歌应属配套使用的乐曲。其乐名为"邹子乐",当系邹衍创作,表达的思想主旨是阴阳五行家的四时教令思想。《迎四时》产生于战国秦汉之际四时与五行两种历法整合的时期,本为齐地民间祭祀四时主时所用,汉武帝时被用作天子郊祭礼之乐。《迎四时》由民俗上升为王礼后,从国家层面强化了民众的四时观念,助推了四时历的最终确定。

关键词：银雀山汉简《迎四时》四时历五行历

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汽车永磁同步电机切换函数式混合控制策略

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汽车工程 2023年第4期45卷 619-626,597页

作者：吴志恒刘爱民沈阳工业大学电气工程学院沈阳110000

为提高电动汽车所配永磁同步电机(PMSM)的驱动系统工作效率,增强运动过程的平稳性及响应速度,以达到提升电动汽车驱动系统的整体动态控制性能。根据电动汽车电机工作特性分析及反馈控制原理推导,提出并设计切换函数式混合控制技术。该... 详细信息

为提高电动汽车所配永磁同步电机(PMSM)的驱动系统工作效率,增强运动过程的平稳性及响应速度,以达到提升电动汽车驱动系统的整体动态控制性能。根据电动汽车电机工作特性分析及反馈控制原理推导,提出并设计切换函数式混合控制技术。该控制技术有效地提高了车用电机控制系统的动、静态性能和鲁棒性。为验证所提控制技术的有效性,建立仿真模型对其进行仿真分析,并搭建实验平台进行实验验证。仿真与实验结果均表明,所提控制技术具有输出响应快、无超调和振荡的优点,能够提高电机工作效率,优化驱动系统输出特性,提升电动汽车驱动系统的控制性能。

关键词：永磁同步电机电动汽车模糊比例控制切换函数

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