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genetic Diversity of Rice Landraces from Lowland and Upland Accessions of china

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Rice science 2013年第4期20卷 259-266页

作者： ZHANG li-na CAO Gui-lan HAN long-zhi institute of crop sciences Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Germplasm Resources and UtilizationMinistry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic Improvement

genetic diversity of rice landraces from lowland and upland accessions of china was investigated using 66 polymorphic simple sequence repeat （SSR） markers. The total number of alleles detected from all 324 tested accessions was 555 with an average allele number （Na） of 8.409 per locus, the average effective number of alleles （Ne） of 3.574 and the average Shannon＇s information index （I） of 1.378. The genetic diversity was higher for the indica landraces compared to the japonica landraces, and the upland landraces were more genetically diverse than the lowland landraces. The SSR markers, RM72, RM232, RM219, RM241, RM224 and RM3 showed the highest rates of polymorphism and these SSR markers were suitable to assess the genetic diversity of rice germplasm resources. A dendrogram of 324 accessions of lowland and upland landraces showed that all rice accessions were mainly subdivided into two groups, japonica and indica, with some being intermediate. The distribution of lowland and upland landraces among the japonica and indica rice groups was distinct, with obvious differentiation between the lowland and upland landraces in japonica rice, but no such clear distinction in indica rice.

关键词： lowland rice upland rice rice landrace genetic diversity simple sequence repeat marker

Establishment and application of an accurate identification method for fragrant soybeans

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Journal of Integrative Agriculture 2021年第5期20卷 1193-1203页

作者： ZHANG Yong-fang ZHANG Chun-yan ZHANG Bo YIN Man HONG Hui-long YU li-li GAO Hua-wei GU Yong-zhe liU Zhang-xiong li Fu-heng QIU li-juan College of life sciences Northeast Agricultural UniversityHarbin 150030P.R.China College of life sciences Shanxi Datong UniversityDatong 037009P.R.China national key facility for crop gene resources and genetic improvement(NFCRI)/key Laboratory of Germplamsm Utilization Ministry of Agriculture and Rural Affairs/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China School of Plant and Environmental sciences Virginia Polytechnic Institute and State UniversityBlacksburgVA 24060USA

In order to screen the aroma characteristics of soybean,a new method was established which can quickly quantify the content of 2-acetyl-1pyrroline(2-AP),an important compound related to soybean aroma,using gas chromatography-mass spectrometry(GC-MS).Based on peak profile,total peak area and retention time as test indexes,an accurate identification method for fragrant soybeans was *** optimum parameters of the protocol consisted of column temperature70℃,sample injector temperature 180℃,optimum extraction alcohol content 1 m L,Na Cl content 0.1 g,ultrasonication time10 min,and extraction time 1 h,which were established by using the orthogonal test of single factors and three factors with four levels(L_9(3)^4).2-AP content of leaves had significant correlations with seeds,which were easier to *** protocol was simple and easy to carry out,consumed only small amounts of reagents,and provided accurate and reliable results with good reproducibility.A total of 101 soybean genotypes from different geographical sources were analyzed using this *** results showed that the average content of 2-AP was 0.29 mg L^(–1),ranging from 0.094 to 1.816 mg L^(–1),and the genetic diversity index was *** all genotypes-tested,they were classified into three grades,including seven elite genotypes identified as"grade one fragrant soybeans",which were Zhonglong 608,Heinong 88,Ha13-2958,Hongmiandou,Heinong 82,Huangmaodou,and Jiyu *** results provide both an identification technique and several elite aroma genotypes for gene discovery and good quality breeding in soybean.

关键词： soybean 2-acetyl-1pyrroline GC-MS quantification method germplasm

大豆巢式关联作图(NAM)群体构建及花色和种皮色遗传分析

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作物学报 2024年第3期50卷 556-575页

作者：宋健熊亚俊陈伊洁徐瑞新刘康林郭庆元洪慧龙高华伟谷勇哲张丽娟郭勇阎哲刘章雄关荣霞李英慧王晓波郭兵福孙如建闫龙王好让姬月梅常汝镇王俊邱丽娟长江大学湖北荆州434025 中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程/农业农村部北京大豆生物学重点实验室北京100081 安徽农业大学农学院安徽合肥230036 江西省农业科学院作物研究所江西南昌330200 呼伦贝尔市农业科学研究所内蒙古呼伦贝尔162650 农业农村部长江中游作物绿色高效生产重点实验室(部省共建)/长江大学农学院湖北荆州434025 河北省农林科学院粮油作物研究所河北石家庄050035 江苏徐怀地区徐州农业科学研究所江苏徐州210031 宁夏农林科学院作物研究所宁夏银川750021

巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发... 详细信息

巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。

关键词：大豆 NAM群体花色种皮色遗传分析

Construction of Double Right-Border Binary Vector Carrying Non-Host gene Rxo1 Resistant to Bacterial Leaf Streak of Rice

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Rice science 2008年第3期15卷 243-246页

作者： Xu Mei-rong XIA Zhi-hui ZHAI Wen-xue Xu Jian-long ZHOU Yong-li li Zhi-kang national key facility for crop genetic resources ＆ improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

Rxol cloned from maize is a non-host gene resistant to bacterial leaf streak of rice. pCAMBIA1305-1 with Rxo1 was digested with Sca I and NgoM IV and the double right-border binary vector pMNDRBBin6 was digested with Hpa I and Xma I. pMNDRBBin6 carrying the gene Rxo1 was acquired by ligation of blunt-end and cohesive end. The results of PCR, restriction enzyme analysis and sequencing indicated that the Rxo1 gene had been cloned into pMNDRBBin6. This double right-border binary vector, named as pMNDRBBin6-Rxol, will play a role in breeding marker-free plants resistant to bacterial leaf streak of rice by genetic transformation.

关键词： Rxo1 gene double right-border binary vector rice bacterial leaf streak selectable marker-free plant resistance gene

Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2014年第8期13卷 1609-1620页

作者： CHAI Lu ZHANG Jian PAN Xiao-biao ZHANG Fan ZHENG Tian-qing ZHAO Xiu-qing WANG Wen-sheng Ali Jauhar XU Jian-long li Zhi-kang national key facility for crop gene resources and genetic improvement/institute of crop sciences Chinese Academy of Agricultural Sciences crop Research institute Taizhou Academy of Agricultural Sciences of Zhejiang Province International Rice Research institute DAPO 777 Metro Malina The Philippines

Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the wholeplant growth duration salt tolerance（ST） was genetically dissected by phenotyping two sets of BC2F5 introgression lines（ILs） for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL（AB-QTL） analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and nonstress conditions were identified, most（〉90%） of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan（HHZ） ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection（MAS） or genome selection.

关键词： rice whole plant growth duration salt tolerance quantitative trait loci(QTLs) epistasis

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东乡野生稻与日本晴多态性标记的开发

作物学报 2019年第2期45卷 316-321页

作者：马小定唐江红张佳妮崔迪李慧黎毛毛韩龙植中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程北京100081 重庆师范大学重庆401331 江西省农业科学院水稻研究所江西南昌330200

东乡野生稻是分布于全球最北端的一种普通野生稻,与亚洲栽培稻基因组差异较大,目前缺少覆盖其全基因组的分子标记。本文以东乡野生稻和日本晴为材料,通过筛选已有的1017个标记,并利用东乡野生稻基因组重测序信息设计的217个InDel标记,... 详细信息

东乡野生稻是分布于全球最北端的一种普通野生稻,与亚洲栽培稻基因组差异较大,目前缺少覆盖其全基因组的分子标记。本文以东乡野生稻和日本晴为材料,通过筛选已有的1017个标记,并利用东乡野生稻基因组重测序信息设计的217个InDel标记,共检测出203个标记在东乡野生稻与日本晴间呈现多态性。这些标记均匀分布于12条染色体,平均间隔1.9 Mb,基本覆盖东乡野生稻全基因组区域。通过对籼粳亚种的检验分析,发现该套多态性分子标记在东乡野生稻与粳稻杂交后代群体基因型分析上具有较高的应用价值。本研究结果为发掘东乡野生稻的有利基因以及分子标记辅助选择育种提供了有力的工具。

关键词： InDel标记基因组重测序粳稻

A Dominant Locus, qBSC-1, Controls β Subunit Content of Seed Storage Protein in Soybean(Glycine max(L.) Merri.)

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Journal of Integrative Agriculture 2014年第9期13卷 1854-1864页

作者： WANG Jun liU lin GUO Yong WANG Yong-hui ZHANG Le JIN long-guo GUAN Rong-xia liU Zhang-xiong WANG lin-lin CHANG Ru-zhen QIU li-juan national key facility for gene resources and genetic improvement (NFCRI)/institute of crop sciences Chinese Academy of Agricultural Sciences

Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines（RHL） derived from the cross between Yangyandou（low level of β subunit） and Zhonghuang 13（normal level of β subunit） were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1（β subunit content）, which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome（Glyma 1.0, Williams 82）, including the two copies of β subunit coding gene CG4. An InDel marker developed from a thymine（TT） insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection（MAS）.

关键词： soybean seed storage protein β subunit QTL mapping

A Comparative Study on Mutagenic Effects of Space Flight and Irradiation of γ-rays on Rice

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agricultural sciences in china 2006年第11期5卷 812-819页

作者： WEI li-jun XU Jian-long WANG Jun-min YANG Qian LUO Rong-ting ZHANG Ming-xian BAO Gen- liang SUN Ye-qing Department of life Science and Engineering College of Science Harbin Institute of Technology Harbin 150001 P.R.China The institute of crop sciences/The national key facility for crop gene resources and genetic improvement Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China institute of crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R.China

The experiment was conducted to study the mutagenic effects of space environment on seedling growth in M1 generation and plant height and heading date in M2 generation in rice. Two types of lines of growth promotion （GP） and growth suppression （GS） were selected from the recombinant inbred lines （RILs） derived from a cross between a sensitive japonica Lemont and a nonsensitive indica Teqing to compare the mutation frequency and mutagenic efficiency for plant height and heading date in the M2 generation. Space environment resulted in 34.9% higher seedling height （SH） in the GP group than in the control, and 39.1% lower in the GS group than in the control, and there was no difference in seed fertility （SF） between the two groups. In M2, mutants of plant height and heading date can be induced by space treatment in both the two groups with lower mutation frequency and mutagenic efficiency in the GP group than in the GS group. There were no significant differences in the physiological damages in M1 between the two groups after γ-rays irradiation treatment. Mutation frequency and mutagenic efficiency of heading date in M2 were higher in the GS group than in the GP group, and the opposite was true for mutagenic efficiency of plant height although the mutation frequency varied between the two groups. The mutation frequencies of plant height and heading date induced by space environment were obviously lower than those by γ-rays irradiation, but the mutagenic efficiency was the opposite for most of the traits. For the GP and GS of seedlings induced by space environment, the GS had higher mutation frequency and mutagenic efficiency for plant height and heading date than the GP in M2.

关键词： rice space environment-induced mutation mutagenic effect physiological damage mutation breeding

Detection of Drought-Related Loci in Rice at Reproductive Stage Using Selected Introgressed lines

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agricultural sciences in china 2011年第1期10卷 1-8页

作者： CHEN Man-yuan Ali j FU Bin-ying XU Jian-long ZHAO Ming-fu JIANG Yun-zhu ZHU ling-hua SHI Ying-yao YAO Da-nian GAO Yong-ming li Zhi-kang College of Agronomy Anhui Agricultural University Hefei 230036 P.R.China institute of crop Science/national key facility for crop gene resources and genetic improvement Chinese Academy of Agricultural Sciences （CAAS） Beijing 100081 P.R.China International Rice Research institute Metro Manila DAP Box 7777 Philippines

The present study was carried out to illustrate high-efficient detection of quantitative trait loci （QTLs） with selected introgression lines （ILs） and the existence of ＇hidden genes＇ conferring drought tolerance （DT）. 52 selected DT ILs, derived from BC2Fz population developed by crossing and backcrossing the susceptible recurrent parent （RP） IR64 with the susceptible donor Khazar were planted under irrigation and drought condition. Four important agronomic traits, e.g., grain yield （GY）, heading date （HD）, panicle numbers per plant （PN）, and plant height （PH） were evaluated and 83 SSR polymorphic molecular markers were used for genotypic analysis. Chi-square test based on genetic hitch-hiking and one-way analysis of variances （ANOVA） were used to detect drought-related loci. Nine and 36 loci were detected by chi-square test and one-way ANOVA, respectively. Five common loci were observed by comparing the results of the two methods, among which two QTLs linked with RM7, and RM241 were detected under irrigation condition, both of the favorable alleles were from RP and explained 13% phenotypic variation （PV） for GY and 28% PV for PH, respectively. The other three QTLs linked with RM 163, RM 18, and RM270 were detected under drought condition, the favorable alleles were all from the donor and explained 10, 24, and 19% PV for HD, PH, and PH, respectively. Five common loci were observed by comparing the results of chi-square test and one-way ANOVA including two QTLs （one for GY and one for PH） under irrigation condition and three QTLs （one for HD and two for PH） under drought condition. By combining phenotypic and genotypic analysis, drought escape could be inferred as the main mechanism for drought tolerance in the present study. The results in present study suggested that the selected ILs population analyzed by chi-square test and one-way ANOVA was quite effective for DT QTL detection with low inputs and could also produce useful materials for breeding with wide genetic

关键词： rice drought tolerance introgression lines (ILs) quantitative trait loci (QTL) hidden diversity