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The OsWRKY72-OsAAT30/OsGSTU26 module mediates reactive oxygen species scavenging to drive heterosis for salt tolerance in hybrid rice

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Journal of Integrative Plant Biology 2024年第4期66卷 709-730页

作者： Citao liu Bigang Mao Yanxia Zhang Lei tian Biao Ma Zhuo Chen Zhongwei Wei aifu li Ye Shao Gongye Cheng lingling li Wenyu li Di Zhang Xiaoping Ding Jiangxiang Peng Yulin Peng Jiwai He Nenghui Ye Dingyang Yuan Chengcai Chu Meijuan Duan Hunan Provincial Key Laboratory of Stress Biology College of AgricultureHunan Agricultural UniversityChangsha 410128China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125China College of Agriculture South China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Wheat and Maize Crop Science Center for Crop Genome EngineeringCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMI 48109 USA Biobin Data Science Co. Ltd.Changsha 410221China

Hybrid rice(Oryza sativa)generally outperforms its inbred parents in yield and stress tolerance,a phenomenon termed heterosis,but the underlying mechanism is not completely ***,we combined transcriptome,proteome,physiological,and heterosis analyses to examine the salt response of super hybrid rice Chaoyou1000(CY1000).In addition to surpassing the mean values for its two parents(mid-parent heterosis),CY1000 exhibited a higher reactive oxygen species scavenging ability than both its parents(over-parent heterosis or heterobeltiosis).Nonadditive expression and allele-specific gene expression assays showed that the glutathione S-transferase gene OsGSTU26 and the amino acid transporter gene OsAAT30 may have major roles in heterosis for salt tolerance,acting in an overdominant fashion in ***,we identified OsWRKY72 as a common transcription factor that binds and regulates OsGSTU26 and *** salt-sensitive phenotypes were associated with the OsWRKY72^(paternal)genotype or the OsAAT30^(maternal)genotype in core rice germplasm ***72^(paternal)specifically repressed the expression of OsGSTU26 under salt stress,leading to salinity sensitivity,while OsWRKY72^(maternal)specifically repressed OsAAT30,resulting in salinity *** results suggest that the OsWRKY72-OsAAT30/OsGSTU26 module may play an important role in heterosis for salt tolerance in an overdominant fashion in CY1000 hybrid rice,providing valuable clues to elucidate the mechanism of heterosis for salinity tolerance in hybrid rice.

关键词： allele-specific gene expression(ASE) heterosis hybrid rice nonadditive effect(NAE) salt tolerance transcriptome and proteome

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Modulation of nitrate-induced phosphate response by the MYB transcription factor Rli1/HINGE1 in the nucleus

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Molecular Plant 2021年第3期14卷 517-529页

作者： Zhihua Zhang Zhao li Wei Wang Zhimin Jiang liping Guo Xiaohan Wang Yangwen Qian Xiahe Huang Yongqiang liu Xiujie liu Yahong liu aifu li Yu Yan Junpeng Xie Shouyun Cao Stanislav Kopriva Legong li Fanjiang Kong Baohui liu Yingchun Wang Bin Hu Chengcai Chu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina School of life Sciences Guangzhou University.GuangzhouChina College of Plant Science Jilin UniversityChangchunChina Biogle Genome Editing Center ChangzhouChina State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina College of Advanced Agricultural Science University of Chinese Academy of SciencesBeijingChina Botanical Institute Cluster of Excellence on Plant SciencesUniversity of CologneCologneGermany College of life Sciences Capital Normal UniversityBeijingChina

The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal ***,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in *** this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as Rli1),a MYB-transcription factor closely related to ***1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced *** importantly,Rli1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate ***,Rli1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.

关键词： rice NIPR Rli1/HINGE1 PHR2 SPXs nucleus

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Melatonin regulates root architecture by modulating auxin response in rice

Melatonin regulates root architecture by modulating auxin re...

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中国生物工程学会第十一届学术年会暨2017年全国生物技术大会

作者： Chengzhen liang aifu li Hua Yu Wenzhen li Chengzhi liang Sandui Guo Rui Zhang Chengcai Chu Biotechnology Research Institute (BRI) Chinese Academy of Agricultural Sciences (CAAS) State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) Institute of Genetics and Developmental Biology (IGDB) Chinese Academy of Sciences (CAS)

It has been suggested that melatonin acts as an important regulator in controlling root growth and development, but the underlying molecular mechanism driving this relationship remains undetermined. In this study,we demonstrated that melatonin acts as a potent molecule to govern root architecture in rice. Treatments with melatonin significantly inhibited embryonic root growth, and promoted lateral root formation and development. Genome-wide expression profiling by RNA-sequencing revealed auxin-related genes were significantly activated under melatonin treatment. Moreover, several transcription factors and candidate cis-regulatory elements involved in root growth and developments, as well as auxin-related processes, were over-represented in both co-up and-down differentially expressed genes, suggesting that melatonin-mediated root growth occurs in an auxin signal pathway-dependent manner. Further,gravitropic response analysis determined that melatonin affects auxin-regulated processes in rice root. These data show that melatonin shapes root architecture by directly or indirectly activating the auxin signaling pathway.

关键词： root growth and development root architecture melatonin auxin transcriptome rice

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Ursodeoxycholic acid breaks antitumor immunosuppression by inducing CHIP-mediated TGF-β degradation

Ursodeoxycholic acid breaks antitumor immunosuppression by i...

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第十四届全国免疫学学术大会

作者： Yingying Shen chaojie Lu zhenbo Song chenxiao Qiao jiaoli Wang jianbiao Chen chengyan Zhang zeyu Ma tao Chen xu li aifu li jufeng Guo jianli Wang Zhijian Cai nstitute of Immunology and Department of Orthopaedics of the Second Affiliated Hospital Zhejiang University School of Medicine Zhejiang University School of Medicine Department of Medical Oncology Zhejiang Cancer Hospital Institute of Immunology and Department of Orthopaedics of the Second Affiliated Hospital Zhejiang University School of Medicine Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province Affiliated Hangzhou First People's Hospital Zhejiang University School of Medicine Zhejiang University Cancer Centre Department of oncology Hangzhou Xixi Hospital Department of Orthopedics Musculoskeletal Tumor Center The Second Affiliated Hospital Zhejiang University School of Medicine School of life Science Westlake University College of life Sciences Zhejiang University Department of Breast Surgery Affiliated Hangzhou First People’s Hospital Zhejiang University School of Medicine Institute of Immunology and Bone Marrow Transplantation Center of the First Affiliated Hospital Zhejiang University School of Medicine Institute of Hematology Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy

Blocking TGF-β signaling is critical to enhance antitumor immunity. However, there is still no effective TGF-β signaling inhibitors in clinic. Here, we show that clinical medicine ursodeoxycholic acid(UDCA) phosphorylates TGF-β at T282 site via TGR5-cAMP-PKA axis, leading to the increased binding of TGF-β and CHIP. Then, CHIP ubiquitinates TGF-β at K315 site, initiating p62-dependent autophagy sorting and subsequent TGF-β degradation. By degrading TGF-β, UDCA greatly enhances antitumor immunity through restraining Treg differentiation and activation in tumor-bearing mice. In addition, UDCA synergizes with anti-PD-1 to induce stronger antitumor immunity and tumor-specific immune memory in tumor-bearing mice. Notably, UDCA also reduces TGF-β and Tregs in peripheral blood of healthy volunteers and tumor patients. Therapy combining anti-PD-1 or anti-PD-L1 with UDCA has much better efficacy in tumor patients. Thus, our Results uncover a novel mechanism for TGF-β signaling regulation and reveal UDCA as a ready-made TGF-β signaling inhibitor in enhancing antitumor immunity.

关键词： ursodeoxycholic acid TGF-β1 signaling TGR5-cAMP-PKA axis Tumor immunity

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脊髓灰质炎后综合征二例

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脑与神经疾病杂志 2019年第6期27卷 336-342页

作者：曹翠芳周晓萌李爱伏李亚坤刘亚玲河北医科大学第二医院神经内科

目的探讨脊髓灰质炎后综合征(PPS)的临床表现及电生理特征,以利于该病的诊断水平。方法收集河北医科大学第二医院神经内科就诊的2例PPS患者的临床资料,分析该病的主要临床表现及电生理特点。结果例1患者7岁时患脊髓灰质炎,遗留右下... 详细信息

目的探讨脊髓灰质炎后综合征(PPS)的临床表现及电生理特征,以利于该病的诊断水平。方法收集河北医科大学第二医院神经内科就诊的2例PPS患者的临床资料,分析该病的主要临床表现及电生理特点。结果例1患者7岁时患脊髓灰质炎,遗留右下肢短小。60年后出现缓慢进展的左下肢无力,肌肉萎缩;例2患者3岁时患脊髓灰质炎,遗留双下肢屈曲畸形,脊柱向右侧弯曲。47年后出现双上肢进行性无力,肌肉萎缩,疼痛,疲乏等表现,2例患者肌电图检查均以轻收缩时运动单位电位时限增宽及电位波幅增高为主,安静状态下自发电位少见。结论 PPS是脊髓灰质炎发病后,临床症状稳定多年后再次出现缓慢进展的迟缓性瘫痪,肌电图主要表现为慢性的神经再生过程。

关键词：脊髓灰质炎后综合征肌肉萎缩疲劳感疼痛神经电生理

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新PEDV流行毒株的病理学观察及抗原定位研究

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黑龙江畜牧兽医 2017年第10期 168-169,296页

作者：王爱富李桂珍黄冬妮付强罗惠娜马春全佛山科学技术学院广东佛山528231

为了加深对猪流行性腹泻病理学损害的认识,试验采用常规石蜡切片及免疫组织化学方法对确诊为变异猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)引起的患病仔猪主要脏器的病理变化及抗原定位进行了研究。结果表明:猪流行性腹... 详细信息

为了加深对猪流行性腹泻病理学损害的认识,试验采用常规石蜡切片及免疫组织化学方法对确诊为变异猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)引起的患病仔猪主要脏器的病理变化及抗原定位进行了研究。结果表明:猪流行性腹泻患病仔猪胃黏膜脱落,肠系膜肿胀充血、乳糜管消失;小肠绒毛上皮细胞坏死、脱落,肠绒毛缩短,肠壁变薄;肠系膜淋巴结髓质区瘀血出血;小肠绒毛上皮细胞、肠腺细胞、隐窝部位、小肠绒毛固有层和肠系膜淋巴结出现PEDV强阳性表达。说明PEDV的主要靶器官是胃肠黏膜上皮、腺体及相应的淋巴结,而胃、肠等器官的损伤是该病的主要病理变化特征。

关键词：猪流行性腹泻(PED) 病理学免疫组化抗原定位

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安钢水冶炼铁技术的进步

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钢铁 2000年第9期35卷 10-12页

作者：李栓紧万爱福郭春喜安钢水冶钢铁有限责任公司

回顾了水冶钢铁公司近 10年来在精料、工艺装备、高炉操作及环境保护方面的技术进步。

关键词：炼铁技术进步精料工艺装备环境保护

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AIFM1 variants associated with auditory neuropathy spectrum disorder cause apoptosis due to impaired apoptosis-inducing factor dimerization

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第2期24卷 172-184页

作者： YUE QIU HONGYANG WANG HUAYE PAN JING GUAN LEI YAN MINGJIE FAN HUI ZHOU XUANHAO ZHOU KAIWEN WU ZEXIAO JIA QIANQIAN ZHUANG ZHAOYING LEI MENGYAO li XUE DING aifu liN YONG FU DONG ZHANG QIUJU WANG QINGFENG YAN College of life Sciences Zhejiang UniversityHangzhou 310058China Senior Department of Otolaryngology Head and Neck SurgeryChinese PLA Institute of OtolaryngologyChinese PLA General HospitalBeijing 100853China Department of Pediatrics the First Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310003China The Children's Hospital of Zhejiang University School of Medicine Hangzhou 310052China Key Laboratory for Cell and Gene Engineering of Zhejiang Province Hangzhou 310058China

Auditory neuropathy spectrum disorder(ANSD)represents a variety of sensorineural deafness conditions characterized by abnormal inner hair cells and/or auditory nerve function,but with the preservation of outer hair cell *** represents up to 15%of individuals with hearing *** mutation screening,bioinformatic analysis and expression studies,we have previously identified several apoptosis-inducing factor(AIF)mitochondria-associated 1(AIFM1)variants in ANSD families and in some other sporadic ***,to elucidate the pathogenic mechanisms underlying each AIFM1 variant,we generated AIF-null cells using the clustered regularly interspersed short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system and constructed AIF-wild type(WT)and AIF-mutant(mut)(p.T260A,p.R422W,and p.R451Q)stable transfection cell *** then analyzed AIF structure,coenzyme-binding affinity,apoptosis,and other *** revealed that these variants resulted in impaired dimerization,compromising AIF *** reduction reaction of AIF variants had proceeded slower than that of *** average levels of AIF dimerization in AIF variant cells were only 34.5%-49.7%of that of AIF-WT cells,resulting in caspase-independent *** average percentage of apoptotic cells in the variants was 12.3%-17.9%,which was significantly higher than that(6.9%-7.4%)in ***,nicotinamide adenine dinucleotide(NADH)treatment promoted the reduction of apoptosis by rescuing AIF dimerization in AIF variant *** findings show that the impairment of AIF dimerization by AIFM1 variants causes apoptosis contributing to ANSD,and introduce NADH as a potential drug for ANSD *** results help elucidate the mechanisms of ANSD and may lead to the provision of novel therapies.

关键词： Auditory neuropathy spectrum disorder Apoptosis-inducing factor(AIF)mitochondria-associated 1(AIFM1)variants Dimerization Caspase-independent apoptosis Nicotinamide adenine dinucleotide(NADH)treatment

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TaqMan实时荧光PCR检测菰米成分的方法建立

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食品工业科技 2022年第21期43卷 344-349页

作者：杨爱馥万超刘雪华梁冰郑秋月姜丽大连海关辽宁大连116001 大连民族大学辽宁大连116600

本研究建立了一种能够快速检测食品中菰米成分的TaqMan实时荧光PCR方法。以菰米核糖体内转录间隔区(internal transcribed space, ITS)基因为检测靶标,设计特异性引物和探针,建立菰米成分的实时荧光PCR检测方法,并进行物种特异性分析、... 详细信息

本研究建立了一种能够快速检测食品中菰米成分的TaqMan实时荧光PCR方法。以菰米核糖体内转录间隔区(internal transcribed space, ITS)基因为检测靶标,设计特异性引物和探针,建立菰米成分的实时荧光PCR检测方法,并进行物种特异性分析、灵敏度分析以及实际应用检测。结果显示:本研究所建立的实时荧光PCR检测方法特异性强,仅对菰米品种中国菰(Z. latifolia)、水生菰(Z. aquatica)、沼生菰(Z. palustris)和德克萨斯菰(Z. texana)基因组DNA出现特异性扩增曲线,供试的其他30种谷物以及异源性动植物基因组DNA均无扩增曲线;该方法对菰米成分检测的灵敏度为0.001 ng/μL菰米基因组DNA或0.01%(W/W)菰米粉。应用该方法对100份市售的进口菰米、菰米碎米、杂粮米及混合米粉等样品进行检测,在80份进口菰米和5份菰米碎米中检测出菰米成分,其他样品中未检出菰米成分,与商品标识一致。该方法灵敏度高,特异性强,可快速高效地进行菰米成分的真实性甄别。

关键词：菰米 ITS基因 TaqMan实时荧光PCR 快速检测真实性鉴别

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麦克卢汉技术思想研究——兼及与雅斯贝斯技术思想的比较

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湖南工业大学学报（社会科学版） 2013年第1期18卷 102-105页

作者：李中华姜爱福湖南工业大学文新学院湖南株洲412007 株洲电信分公司湖南株洲412007

作为20世纪著名文化思想家,麦克卢汉十分重视技术给社会历史带来的深刻影响。在麦克卢汉看来,(1)技术的实质乃是媒介,是人的延伸;(2)技术的变革带来文化的变迁;(3)技术变迁最重要的影响还包括:导致人们感知的变化和创造"环境"。和雅斯... 详细信息

作为20世纪著名文化思想家,麦克卢汉十分重视技术给社会历史带来的深刻影响。在麦克卢汉看来,(1)技术的实质乃是媒介,是人的延伸;(2)技术的变革带来文化的变迁;(3)技术变迁最重要的影响还包括:导致人们感知的变化和创造"环境"。和雅斯贝斯一样,他们都十分关注技术的社会文化意义,具有强烈的人文精神。麦克卢汉的深刻之处在于,他将技术视为社会的存在方式,超越了雅斯贝斯单纯的技术"工具论"范畴。

关键词：麦克卢汉雅斯贝斯技术思想本体论工具论

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