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右江医学 2014年第2期42卷 198-200页

作者：李凤莲黄艾芬广西河池市第一人民医院新生儿科宜州546300

目的探讨早期护理干预对促进新生儿缺氧缺血性脑病(HIE)智能发育的临床效果。方法结合家长意愿将96例HIE患儿分成观察组50例及对照组46例,对照组实施常规护理,观察组在此基础上实施早期护理干预,定期进行随访,比较两组患儿不同年龄段智... 详细信息

目的探讨早期护理干预对促进新生儿缺氧缺血性脑病(HIE)智能发育的临床效果。方法结合家长意愿将96例HIE患儿分成观察组50例及对照组46例,对照组实施常规护理,观察组在此基础上实施早期护理干预,定期进行随访,比较两组患儿不同年龄段智能发育情况及脑瘫发生率。结果观察组3个月、6个月及12个月时智能发育商显著优于对照组(P<0.01),随访2年后,脑瘫发生率为6.38%,显著低于对照组的40.91%(P<0.01)。结论早期护理干预可促进HIE患儿的智能发育,降低脑瘫发生率,是一种效果确切的护理干预措施。

关键词：早期护理干预新生儿缺氧缺血性脑病智能发育临床效果

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儿童幽门螺杆菌感染与缺铁性贫血的相关性

临床医学 2007年第4期27卷 79-80页

作者：徐辉朱欢黄爱芬胡伟国程芹芹黄开宇温州医学院育英儿童医院消化科温州325027

目的探讨儿童中幽门螺杆菌(Hp)感染与缺铁性贫血(IDA)的相关性。方法以因慢性腹痛而行胃镜检查及Hp检测的60例患儿为观察对象,根据检查结果分为Hp阳性慢性胃炎组、Hp阴性慢性胃炎组及Hp阳性胃部镜下无损害组三组。所有病例均检测血红蛋... 详细信息

目的探讨儿童中幽门螺杆菌(Hp)感染与缺铁性贫血(IDA)的相关性。方法以因慢性腹痛而行胃镜检查及Hp检测的60例患儿为观察对象,根据检查结果分为Hp阳性慢性胃炎组、Hp阴性慢性胃炎组及Hp阳性胃部镜下无损害组三组。所有病例均检测血红蛋白(Hb)、平均红细胞体积(MCV)、血清铁(SI)、血清铁蛋白(SF)、总铁结合力(TIBC)等IDA指标。比较三组之间的IDA指标和IDA伴发率。结果三组病例IDA指标两两比较显示,Hp阴性慢性胃炎组分别和Hp阳性慢性胃炎组、Hp阳性胃部镜下无损害组的IDA指标比较差异均有统计学意义(P0.05)。Hp阴性慢性胃炎的IDA伴发率均明显低于Hp阳性慢性胃炎组和阳性胃部镜下无损害组(P0.05)。结论儿童幽门螺杆菌感染与缺铁性盆血两者之间具有显著的相关性,其相关性较慢性胃炎更为密切。

关键词：儿童幽门螺杆菌缺铁性贫血

行为干预在小儿出口梗阻型便秘治疗中的临床作用

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临床医学 2008年第2期28卷 28-30页

作者：徐辉徐彰周晓青潘彤彤黄爱芬温州医学院育英儿童医院消化科浙江温州325027

目的探讨行为干预措施在小儿功能性出口梗阻型便秘治疗中对临床症状改善的作用。方法对38例确诊为出口梗阻型便秘的患者进行便秘症状评分,并随机分为干预组与对照组,对照组给予一般药物治疗,干预组在对照组的基础上给予合理饮食、适量... 详细信息

目的探讨行为干预措施在小儿功能性出口梗阻型便秘治疗中对临床症状改善的作用。方法对38例确诊为出口梗阻型便秘的患者进行便秘症状评分,并随机分为干预组与对照组,对照组给予一般药物治疗,干预组在对照组的基础上给予合理饮食、适量饮水、调整生活习惯、适当运动、精神减负、腹部按摩及排便训练等一系列行为干预措施辅助治疗,治疗后两组病例再次进行便秘症状评分,并比较评分结果。结果干预组治疗后的直肠初始感觉值与最大耐受值明显低于本组治疗前及对照组(P0.05),治疗后干预组的便秘临床评分明显低于对照组(P<0.01)。结论通过行为指导与训练配合药物治疗能明显减轻出口梗阻型便秘患儿的临床症状,提高疗效,达到单纯药物所不能替代的作用。

关键词：行为干预小儿出口梗阻型便秘

不同临床类型儿童功能性消化不良与幽门螺杆菌感染的关系

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临床医学 2007年第3期27卷 22-23页

作者：黄开宇卢华君陈桧平徐辉黄爱芬朱欢朱世钗温州医学院附属育英儿童医院浙江温州325000

目的探讨儿童功能性消化不良(FD)临床特点,以及儿童FD不同亚型的胃排空和幽门螺杆菌(Hp)感染情况。方法对167例FD儿童以问卷形式询问病史,按症状给予分型。通过B超了解胃液体排空功能,通过胃窦黏膜组织病理和13C尿素呼气试验检测幽门螺... 详细信息

目的探讨儿童功能性消化不良(FD)临床特点,以及儿童FD不同亚型的胃排空和幽门螺杆菌(Hp)感染情况。方法对167例FD儿童以问卷形式询问病史,按症状给予分型。通过B超了解胃液体排空功能,通过胃窦黏膜组织病理和13C尿素呼气试验检测幽门螺杆菌感染情况。结果FD儿童167例,Hp阳性71例,阳性率42.5%。167例中动力障碍型101例,Hp阳性率56.4%;溃疡型38例,Hp阳性率21.0%;非特异型23例,Hp阳性率26.1%;反流型5例,Hp阳性率0;动力障碍型Hp阳性率明显高于其他三型,差异有统计学意义(P<0.05)。动力障碍型Hp阳性组胃液体排空率慢于Hp阴性组,差异有统计学意义(P<0.05)。结论儿童FD中以动力障碍这一亚型最常见。Hp与动力障碍型FD的关系密切。

关键词：功能性消化不良幽门螺杆菌

幽门螺杆菌感染儿童胃粘膜IL-8含量的研究

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临床医学 2006年第5期26卷 69-70页

作者：朱欢黄爱芬陈桧平邵美娟胡伟国黄开宇程芹芹温州医学院附属育英儿童医院浙江温州325027

目的探讨IL-8在儿童幽门螺杆菌(***)感染相关性胃炎中的作用。方法82例患儿在胃镜下取胃窦粘膜活检标本,行组织病理学检查,采用酶联免疫吸附试验(ELISA)法检测胃窦粘膜中IL-8的含量。结果***阳性患儿胃粘膜IL-8含量为(15.2±5.4)pg/mg,... 详细信息

目的探讨IL-8在儿童幽门螺杆菌(***)感染相关性胃炎中的作用。方法82例患儿在胃镜下取胃窦粘膜活检标本,行组织病理学检查,采用酶联免疫吸附试验(ELISA)法检测胃窦粘膜中IL-8的含量。结果***阳性患儿胃粘膜IL-8含量为(15.2±5.4)pg/mg,***阴性患儿胃粘膜IL-8含量为(3.3±1.3)pg/mg,两组比较差异有统计学意义(t=13.315,P<0.001)。不同的***定植密度其IL-8含量差异无统计学意义(t=1.831,P=0.074)。***阳性患儿活动性炎症及淋巴滤泡检出率和阴性患儿比较差异有统计学意义(χ2值分别为12.286、6.413,P均<0.01)。活动性胃炎胃粘膜IL-8含量为(20.0±5.0)pg/mg,非活动性胃炎胃粘膜IL-8含量为(12.8±3.8)pg/mg;有淋巴滤泡形成胃粘膜IL-8含量为(21.1±3.4)pg/mg,无淋巴滤泡形成胃粘膜IL-8含量为(13.5±4.7)pg/mg;差异有统计学意义(t值分别为5.306、4.942,P均<0.01)。结论儿童感染***后胃粘膜上皮组织可检测到高浓度的IL-8,IL-8能引起中性粒细胞浸润及淋巴滤泡形成,提示IL-8在***感染后胃粘膜组织的炎症反应中具有重要作用。

关键词：幽门螺杆菌 IL-8 胃炎儿童

Genetic Evidence that Dorsal Spinal Oligodendrocyte Progenitor Cells are Capable of Myelinating Ventral Axons Effectively in Mice

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Neuroscience Bulletin 2020年第12期36卷 1474-1483页

作者： Minxi Fang Qian Yu Baiyan Ou Hao huang Min Yi Binghua Xie aifen Yang Mengsheng Qiu Xiaofeng Xu Institute of Life Sciences Zhejiang Key Laboratory of Organ Development and RegenerationCollege of Life and Environmental SciencesHangzhou Normal UniversityHangzhou 310029China CAS Center for Excellence in Brain Science and Intelligence Technology Institute of NeuroscienceChinese Academy of SciencesShanghai 200031China

In the developing spinal cord,the majority of oligodendrocyte progenitor cells(OPCs)are induced in the ventral neuroepithelium under the control of the Sonic Hedgehog(Shh)signaling pathway,whereas a small subset of OPCs are generated from the dorsal neuroepithelial cells independent of the Shh *** dors allyderived OPCs(dOPCs)have been shown to participate in local axonal myelination in the dorsolateral regions during development,it is not known whether they are capable of migrating into the ventral region and myelinating ventral *** this study,we confirmed and extended the previous study on the developmental potential of dOPCs in the absence of ventrally-derived OPCs(vOPCs).In NestinSmo conditional knockout(cKO)mice,when ventral oligodendrogenesis was blocked,dOPCs were found to undergo rapid amplification,spread to ventral spinal tissue,and eventually differentiated into myelinating OLs in the ventral white matter with a temporal delay,providing genetic evidence that dOPCs are capable of myelinating ventral axons in the mouse spinal cord.

关键词： Dorsally-derived OPCs OPC proliferation Oligodendrocyte differentiation Myelination

Aberrant nuclear lamina contributes to the malignancy of human gliomas

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Journal of Genetics and Genomics 2022年第2期49卷 132-144页

作者： Shunqi Pei Xuehui Wang Xuan Wang Hao huang Huaping Tao Binghua Xie aifen Yang Mengsheng Qiu Zhou Tan Institute of Life Sciences Key Laboratory of Organ Development and Regeneration of Zhejiang ProvinceCollege of Life SciencesHangzhou Normal UniversityZhejiang 311121China

Glioma is the most common type of tumor in the central nervous system, accounting for around 80% of all malignant brain tumors. Previous studies showed a significant association between nuclear morphology and the malignant progress of gliomas. By virtue of integrated proteomics and genomics analyses as well as experimental validations, we identify three nuclear lamin genes(LMNA, LMNB1, and LMNB2) that are significantly upregulated in glioma tissues compared with normal brain tissues. We show that elevated expressions of LMNB1, LMNB2, and LMNA in glioma cells are highly associated with the rapid progression of the disease and the knockdown of LMNB1, LMNB2, and LMNA dramatically suppresses glioma progression in both in vitro and in vivo mouse models. Moreover, the repression of glioma cell growth by lamin knockdown is mediated by the p Rb-mediated G1-S inhibition. On the contrary, overexpression of lamins in normal human astrocytes dramatically induced nuclear morphological aberrations and accelerated cell growth. Together, our multi-omics-based analysis has revealed a previously unrecognized role of lamin genes in gliomagenesis, providing a strong support for the key link between aberrant tumor nuclear shape and the survival of glioma patients. Based on these findings, lamins are proposed to be potential oncogene targets for therapeutic treatments of brain tumors.

关键词： Gliomas Proteomics Genomics Nucleus Lamins Malignancy

确定海浪要素的风要素及台风气压场的计算方法

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海洋通报 1984年第1期 7-16页

作者：王伯民阎俊岳张秀芝侯书路黄爱芬张荣贞鲍强生李乐贤国家气象局北京气象中心交通部水运规划设计院

在海浪计算中,海浪的方向和高度值的大小,在很大程度上取决于风场和风要素的量值。因而,合理地确定风场和风的量值,是比较准确地进行海浪计算的先决条件。本文就我们在进行青岛前湾波浪计算中,如何从历史天气图计算、分析气压场的分布,... 详细信息

在海浪计算中,海浪的方向和高度值的大小,在很大程度上取决于风场和风要素的量值。因而,合理地确定风场和风的量值,是比较准确地进行海浪计算的先决条件。本文就我们在进行青岛前湾波浪计算中,如何从历史天气图计算、分析气压场的分布,确定风场和计算风速的方法作一简单的叙述。在工作过程中,我们没有直接利用历史天气图进行计算。这是因为,历史天气图中等压线的分析着重于大尺度天气系统的表现特征,没有也不可能对局部地区,特别是缺少记录的

关键词：台风地转风低压(气象) 海浪要素历史天气图气压场台风中心气压等压线热力学性质表梯度风计算方法

Development of an Agrobacterium-mediated CRISPR/Cas9 gene editing system in jute(Corchorus capsularis)

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The Crop Journal 2024年第4期12卷 1266-1270页

作者： Shaolian Jiang Qin Li Xiangxue Meng Mengxin huang Jiayu Yao Chuanyu Wang Pingping Fang aifen Tao Jiantang Xu Jianmin Qi Shuangxia Jin Liwu Zhang Key Laboratory of Ministry of Education for Genetic Breeding and Multiple Utilization of Crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Experiment Station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/Fujian Public Platform for Germplasm Resources of Bast Fiber Crops/Fujian Provincial Key Laboratory of Crop Breeding by Design/Key Laboratory of Ministry of Agriculture and Rural Affairs for Biological Breeding of Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National Key Lab of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after ***,developing an efficient gene editing system for jute remains a *** this study,the transgenic hairy root system mediated by Agrobacterium rhizogenes strain K599 was developed for Meifeng 4,an elite jute variety widely cultivated in *** transgenic hairy root system for jute was verified by subcellular localization and bimolecular fluorescence complementation(BiFC)*** CHLOROPLASTOS ALTERADOS 1(CcCLA1)gene,which is involved in the development of chloroplasts,was targeted for editing at two sites in Meifeng *** on this hairy root transformation,the gRNA scaffold was placed under the control of cotton ubiquitin GhU6.7 and-GhU6.9 promoters,respectively,to assess the efficiency of gene *** indicated the 50.0%(GhU6.7)and 38.5%(GhU6.9)editing events in the target 2 alleles(gRNA2),but no mutation was detected in the target 1 allele(gRNA1)in transgenic-positive hairy ***1 gene editing at gRNA2 under the control of GhU6.7 in Meifeng 4 was also carried out by Agrobacterium tumefaciens-mediated *** CcCLA1 mutants were albinic,with a gene editing efficiency of 5.3%.These findings confirm that the CRISPR/Cas9 system,incorporating promoter GhU6.7,can be used as a gene editing tool for jute.

关键词： Jute Agrobacterium-mediated transformation Genome editing Hairy root system