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Horticultural Plant Journal 2023年第5期9卷 920-930页

作者： Weiting Liu Lichao Zhang Li Ma Hui Yuan aide wang Key Laboratory of Fruit Postharvest Biology Key Laboratory of Protected HorticultureNational&Local Joint Engineering Research Center of Northern Horticultural Facilities Design&Application TechnologyCollege of HorticultureShenyang Agricultural UniversityShenyangLiaoning 110866China Department of Foreign Language Teaching Shenyang Agricultural UniversityShenyangLiaoning 110866China

Ethylene is the main factor controlling fruit ripening of pear(Pyrus ussuriensis).Ethylene production rate is negatively correlated with fruit shelf life;therefore,it is important to decrease the ethylene levels for optimal fruit storage.Here,we observed that blue light treatment could inhibit ethylene production and promote the expression of ELONGATED HYPOCOTYL 5(PuHY5),a basic leucine zipper domain(bZIP)transcription factor.The following studies showed that PuHY5 could bind to the promoter of ACC synthase 1(PuACS1),a rate-limiting enzyme in ethylene biosynthesis,and inhibit its expression.For pears in which Pu HY5 was silenced,the ethylene production and PuACS1 expression were much higher than those in the control fruit.These results demonstrated that blue light inhibited ethylene production through the induction of Pu HY5 in pear.Our finding provides a new method for prolonging fruit shelf life.

关键词： Pear Pyrus ussuriensis Fruit ripening Blue light Ethylene synthesis PuHY5 PuACS1

Effects of calcium application on apple fruit softening during storage revealed by proteomics and phosphoproteomics

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Horticultural Plant Journal 2022年第4期8卷 408-422页

作者： Yaxiu Xu Jiaojiao Liu Nannan Zang Zepeng Yin aide wang Key Laboratory of Fruit Postharvest Biology Key Laboratory of Protected HorticultureNational and Local Joint Engineering Research Center of Northern Horticultural Facilities Design and Application TechnologyCollege of HorticultureShenyang Agricultural UniversityShenyang 110866 China

Fruit ripening has been reported to be related to calcium(Ca),but the underlying mechanisms by which Ca regulates this process remain largely unknown.In order to study the changes of proteins and enriched phosphopeptides,we conducted TMT labeling,bio-material-based PTM enrichment based on mass spectrometry in Ca-treated‘Golden Delicious’(GD)apple fruit(Malus×domestica).This dataset presents a comprehensive overview of the critical pathways involved in fruit ripening.A total of 47 proteins and 124 phosphoproteins significantly changed in Ca-treated fruit,which are crucial for regulating the cell wall and cytoskeleton,Ca-mediated signaling and transport,ethylene production,protein fate,especially ubiquitination-based protein degradation,and primary and secondary metabolisms.Our results indicated that Ca inhibited the abundance of polygalacturonase(PG)activity and increased the phosphorylation level of CSLD3.PG and phosphorylation were involved in cell wall degradation,thereby delaying fruit softening.As a secondary messenger,Ca-mediated signaling subsequently triggered downstream mitogen-activated protein kinases(MAPK)cascades and activated the membrane,transport,and ROS signaling.Moreover,MdEIN2,a key enzyme involved in the ubiquitin of protein modification,increased at Ser753 and Ser758 in Ca-treated fruit.Furthermore,diverse primary and secondary metabolisms including glycolysis,fatty acid metabolism,and oxidation respiratory chain were modulated to prevent fruit softening.These results provide basic information from protein and phosphorylation levels for apple fruit ripening during storage,which may be helpful for apple fruit storage control.

关键词： Apple Proteomics Phosphoproteomics Calcium Fruit ripening

Soil Profile Concentration Distributions of Antimony and Bismuth across Southeastern Missouri (USA)

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Journal of Environmental Protection 2024年第5期15卷 537-551页

作者： Michael aide Department of Agriculture Southeast Missouri State University Cape Girardeau MO USA

Recent research has suggested that increased industrial and technological utilization of antimony and bismuth necessitates greater research to determine the soil and water chemistry and the environmental risks associated with these elements. The near-total soil profile concentrations of antimony and bismuth were determined for key soil series across southeastern Missouri. The antimony concentrations ranged from 0.65 to 0.08 mg kg−1, whereas the bismuth soil profile concentrations ranged from 0.92 to 0.03 mg kg−1. Most pedons showed antimony concentrations ranging from 20 to 30 mg kg−1, whereas bismuth concentrations were commonly 10 to 20 mg kg−1. For soils having argillic horizons, antimony and bismuth concentrations were greater for the illuvial horizons than the eluvial horizons, whereas Entisols, Inceptisols, and one Vertisol showed rather uniform antimony and bismuth concentrations, features paralleling the soil texture distribution. Both antimony and bismuth showed significant correlations with iron.

关键词： Trace Elements Antimony Bismuth Soils Group 15

Soil Profile Concentration Distributions of Aluminum, Gallium, Indium and Thallium across Southeastern Missouri

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Journal of Geoscience and Environment Protection 2024年第1期12卷 80-92页

作者： Michael aide Department of Agriculture Southeast Missouri State University Cape Girardeau MO USA

The soil chemistry of gallium, indium, and thallium is not well defined, particularly with emerging evidence that these elements have toxic properties and may influence food safety. The purpose of this investigation was to estimate the soil concentrations of gallium, indium, and thallium and determine if these elements have a soil chemistry like aluminum and therefore demonstrate significant concentration correlations with aluminum. Twenty-seven soil series were selected, and the elemental concentrations were determined using aqua regia digestion with analytical determination performed using inductively coupled plasma emission-mass spectroscopy. The concentrations of gallium, indium, and thallium generally compared with the known literature. Aluminum-gallium and aluminum-thallium exhibited significant concentration correlations across the soil horizons of the sampled soils. Aluminum, gallium, and thallium did demonstrate concentration increases in soil horizons having illuviation of phyllosilicates, implying these phyllosilicates have adsorption and isomorphic substitution behaviors involving these elements.

关键词： Trace Elements Gallium Indium Thallium Soils Aluminum

Comparative transcriptome analysis of the climacteric of apple fruit uncovers the involvement of transcription factors affecting ethylene biosynthesis

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Horticultural Plant Journal 2023年第4期9卷 659-669页

作者： Tong Li Xiao Zhang Yun Wei Yaxiu Xu Weiting Liu Hongjian Li Guangxin Yang aide wang Xiaoxue wang Rice Research Institute Shenyang Agricultural UniversityShenyangLiaoning 110866China College of Horticulture Shenyang Agricultural UniversityShenyangLiaoning 110866China Liaoning Institute of Pomology XiongyueLiaoning115009China

Apple(Malus domestica)fruit generally undergoes a climacteric.During its ripening process,there is a peak in ethylene release and its firmness simultaneously decreases.Although more in-depth research into the mechanism of climacteric-type fruit ripening is being carried out,some aspects remain unclear.In this study,we compared the transcriptomes of 0-Pre and 15-Post(pre-and post-climacteric fruit),and 15-Post and 15-MCP[fruit treated with 1-MCP(1-methylcyclopropene)].Various transcription factors,such as MADS-box,ERF,NAC,Dof and SHF were identified among the DEGs(differential gene expressions).Furthermore,these transcription factors were selected for further validation analysis by qRT-PCR.Moreover,yeast one hybrid(Y1H),β-glucuronidase(GUS)transactivation assay and dual-luciferase reporter assay showed that MdAGL30,MdAGL104,MdERF008,MdNAC71,MdDof1.2,MdHSFB2a and MdHSFB3 bound to MdACS1 promoter and directly regulated its transcription,thereby regulating ethylene biosynthesis in apple fruit.Our results provide useful information and new insights for research on apple fruit ripening.

关键词： Apple RNA-Seq Fruit ripening Ethylene Transcription factor

RECENT ADVANCES IN THE REGULATION OF CLIMACTERIC FRUIT RIPENING:HORMONE,TRANSCRIPTION FACTOR AND EPIGENETIC MODIFICATIONS

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Frontiers of Agricultural Science and Engineering 2021年第2期8卷 314-334页

作者： Yinglin JI Mingyang XU aide wang Key Laboratory of Fruit Postharvest Biology of Liaoning Province College of HorticultureShenyang Agricultural UniversityShenyang 110866China.

Fruit ripening is a complex developmental process made up of genetically programmed physiological and biochemical activities.It culminates in desirable changes in the structural and textural properties and is governed by a complex regulatory network.Much is known about ethylene,one of the most important metabolites promoting the ripening of climacteric fruits.However,the dynamic interplay between phytohormones also plays an important part.Additional regulatory factors such as transcription factors(TFs)and epigenetic modifications also play vital role in the regulation of climacteric fruit ripening.Here,we review and evaluate the complex regulatory network comprising interactions between hormones and the action of TFs and epigenetic modifications during climacteric fruit ripening.

关键词： climacteric fruit ripening phytohormones TFs epigenetic modifications

Cloning and Characterization of miRNAs and Their Targets, Including a Novel miRNA-Targeted NBS-LRR Protein Class Gene in Apple （Golden Delicious）

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Molecular Plant 2014年第1期7卷 218-230页

作者： Chao Ma You Lu Songlin Bai Wennan Zhang Xuwei Duan Dong Meng Zhigang wang aide wang Zongshan Zhou Tianzhong Li Laboratory of Fruit Cell and Molecular Breeding China Agricultural University Beijing 100193 China Research Institute of Pomology Chinese Academy of Agricultural Sciences Xingcheng Liaoning 125100 China College of Horticulture Shenyang Agricultural University Shenyang 110866 China

MicroRNA （miRNA） has emerged as an important regulator of gene expression in plants. 146 miRNAs were identified from apple （Malus domestica cv. Golden Delicious） by bioinformatic analysis and RNA library sequencing. From these, 135 were conserved and 11 were novel miRNAs. Target analysis predicted one of the novel miRNAs, Md-miRLn11 （Malus domestica microRNA Ln11）, targeted an apple nucleotide-binding site （NBS）-Ieucine-rich repeat （LRR） class protein coding gene （Md-NBS）. 5/ RACE assay confirmed the ability of Md-miRLn11 to cleave Md-NBS at the 11-12-nt position. Analysis of the expression of Md-miRLn11 and Md-NBS during the optimum invasion period in 40 apple varieties showed that the expression of Md-NBS gene in resistant varieties is higher than in susceptible varieties, with an inverse pattern for Md-miRLn11. Seedlings from the resistant apple variety ＇JiGuan＇ were used to carry out an Agrobacterium infiltration assay, and then inoculated with the apple leaf spot disease. The result showed a clear decline of disease resistance in JiGuan apples. In contrast, the susceptible variety ＇FuJi＇ infiltrated with the Md-NBS gene showed a significant increase in disease resistance. Based on the above results, we propose that Md-miRLn11 regulates Md-NBS gene expression in particular under the condition of pathogen infection, and that the Md-miRLn11 targeting P-loop site may regulate many NBS-LRR protein class genes in woody plants.

关键词： apple microRNA NBS-LRR protein bioinformatics analysis gene function analysis

基于改进型转矩分配函数的开关磁阻电机转矩与峰值电流优化研究

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电机与控制学报 2024年第5期28卷 119-129页

作者：许爱德孙景浩冷冰杨洋大连海事大学信息科学技术学院辽宁大连116026 大连海事大学船舶电气工程学院辽宁大连116026

开关磁阻电机(SRM)使用传统的转矩分配函数控制时由于转矩分配函数的开关角度恒定,使其在换相阶段出现较为明显的转矩脉动和较高的峰值电流现象。针对这一现象,提出实时调节开关角度的指数型转矩分配函数的控制方法来降低转矩脉动与相... 详细信息

开关磁阻电机(SRM)使用传统的转矩分配函数控制时由于转矩分配函数的开关角度恒定,使其在换相阶段出现较为明显的转矩脉动和较高的峰值电流现象。针对这一现象,提出实时调节开关角度的指数型转矩分配函数的控制方法来降低转矩脉动与相峰值电流。与传统的转矩分配控制进行比较分析,所提算法能够在不同电机转速下抑制转矩脉动和降低SRM定子绕组换相峰值电流,在中低速情况下,实时优化转矩分配函数开关角与重叠角,提高了SRM的运行效率。以一台2.2 kW的12/8开关磁阻电机作为控制对象,建立控制系统仿真模型,通过仿真和实验验证了所提算法的有效性。

关键词：开关磁阻电机转矩分配函数开关角度转矩脉动峰值电流

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课程思政下运算放大器的混合式教学探索

电气电子教学学报 2024年第1期46卷 100-103页

作者：许爱德卫海超汪洋樊琨大连海事大学信息科学技术学院大连116026

在理工科核心课程中开展课程思政教育是当前教育发展的新模式。运算放大器是电路理论分析课程中的一个多端元件,对于帮助理解受控源有着非常重要的意义,同时在工程中有着多种实际应用。借助混合式教学这一模式,将思政教育贯彻到运算放... 详细信息

在理工科核心课程中开展课程思政教育是当前教育发展的新模式。运算放大器是电路理论分析课程中的一个多端元件,对于帮助理解受控源有着非常重要的意义,同时在工程中有着多种实际应用。借助混合式教学这一模式,将思政教育贯彻到运算放大器的教学中,在传授知识的同时培养学生的爱国情怀和敬业精神。

关键词：课程思政运算放大器混合式教学

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氨基改性硅油柔软剂的稀释稳定性研究

日用化学工业（中英文） 2024年第4期54卷 425-430页

作者：王艾德崔金德梁丽丽张琳涵曹允灿刘庆润淄博鲁瑞精细化工有限公司山东淄博255000

研究氨基改性硅油柔软剂的稀释稳定性。制备基础硅油化合物,并利用包含氨基的氨基酸对其改性。对氨基改性硅油柔软剂实施稀释倍数下的分层测试、表面张力测试以及透水率测试。结果表明:离心稳定性、耐酸、耐碱稳定性测试下溶液没有出现... 详细信息

研究氨基改性硅油柔软剂的稀释稳定性。制备基础硅油化合物,并利用包含氨基的氨基酸对其改性。对氨基改性硅油柔软剂实施稀释倍数下的分层测试、表面张力测试以及透水率测试。结果表明:离心稳定性、耐酸、耐碱稳定性测试下溶液没有出现分层,而耐热稳定性、耐电解质稳定性两个测试虽然出现分层情况,但是比例系数均在0.1以下,说明经过稀释后,氨基改性硅油柔软剂保持较好的稳定性。随着稀释倍数提高,氨基改性硅油柔软剂的表面张力越来越小,透光率越来越大;随着时间的延长,虽然二者出现波动,但最终基本维持在一定水平上,证明其性能的稳定性。

关键词：氨基改性硅油柔软剂制备工艺分层表面张力透水率稀释稳定性