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Hepatitis B virus persistent infection-related single nucleotide polymorphisms in HLA regions are associated with viral load in hepatoma families

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World Journal of Gastroenterology 2021年第37期27卷 6262-6276页

作者： ai-ru hsieh Cathy S J Fann Hung-Chun Lin Jennifer Tai Sen-Yung hsieh Dar-In Tai Department of Statistics Tamkang UniversityNew Taipei City 25137Taiwan Institute of Biomedical Sciences Academia SinicaNankangTaipei 11529Taiwan Division of Hepatology Department of Gastroenterology and HepatologyChang Gung Memorial HospitalLinkou Medical CenterTaoyuan 33305Taiwan

BACKGROUND Genome-wide association studies from Asia indicate that HLA-DP and HLA-DQ loci are important in persistent hepatitis B virus(HBV)*** of the key elements for HBV-related carcinogenesis is persistent viral replication and *** To examine genetic and nongenetic factors with persistent HBV infection and viral load in families with hepatocellular carcinoma(HCC).METHODS The HCC families included 301 hepatitis B surface antigen(HBsAg)carriers and 424 noncarriers born before the nationwide vaccination program was initiated in *** HBV-related single nucleotide polymorphisms(SNPs)—rs477515,rs9272105,rs9276370,rs7756516,and rs9277535—were *** associated with persistent HBV infection and viral load were analyzed by a generalized estimating *** In the first-stage persistent HBV study,all SNPs except rs9272105 were associated with persistent infection.A significantly higher area under the reciprocal operating characteristic curve for nongenetic factors vs genetic factors(P<0.001)suggests that the former play a major role in persistent HBV *** the second-stage viral load study,we added 8 HBsAg carriers born after *** 309 HBsAg carriers were divided into low(n=162)and high viral load(n=147)groups with an HBV DNA cutoff of 105 cps/***,relationship to the index case,rs477515,rs9272105,and rs7756516 were associated with viral *** on the receiver operating characteristic curve analysis,genetic and nongenetic factors affected viral load equally in the HCC family cohort(P=0.3117).CONCLUSION In these east Asian adults,the mechanism of persistent HBV infection-related SNPs was a prolonged viral replication phase.

关键词： Generalized estimating equation Genetic polymorphism Genome-wide association study Hepatitis B surface antigen Hepatitis B virus Replication

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Effects of sex and generation on hepatitis B viral load in families with hepatocellular carcinoma

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World Journal of Gastroenterology 2017年第5期23卷 876-884页

作者： ai-ru hsieh Cathy SJ Fann Chau-Ting Yeh Hung-Chun Lin Shy-Yi Wan Yi-Cheng Chen Chia-Lin Hsu Jennifer Tai Shi-Ming Lin Dar-In Tai Graduate Institute of Biostatistics China Medical University Institute of Biomedical Sciences Academia Sinica Division of Hepatology Department of Gastroenterology and Hepatology Chang Gung Memorial Hospital Kaohsiung Medical University College of Medicine

aiM To explore factors associated with persistent hepatitis B virus (HBV) infection in a cohort of hepatocellular carcinoma (HCC)-affected families and then investigate factors that correlate with individual viral load among hepatitis B surface antigen (HBsAg)-positive relatives. METHODS questionnaire. Demographics, relationship to index case, HBsAg status of mothers and index cases were evaluated for association with the HBV persistent infection or viral load by generalized estimating equation analysis. RESULTS Among 729 relatives enrolled, parent generation (P = 0.0076), index generation (P = 0.0044), mothers positive for HBsAg (P = 0.0007), and HBsAg-positive index cases (P = 5.98 x 10(-8)) were associated with persistent HBV infection. Factors associated with HBV viral load were evaluated among 303 HBsAg-positive relatives. Parent generation (P = 0.0359) and sex (P = 0.0007) were independent factors associated with HBV viral load. The intra-family HBV viral load was evaluated in families clustered with HBsAg-positive siblings. An intra-family trend of similar HBV viral load was found for 27 of 46 (58.7%) families. Male offspring of HBsAg-positive mothers (P = 0.024) and older siblings were associated with high viral load. CONCLUSION Sex and generation play important roles on HBV viral load. Maternal birth age and nutritional changes could be the reasons of viral load difference between generations.

关键词： Familial generation Sex Hepatitis B virus Perinatal infection Viral replication

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Variation Characteristics of Root Traits of Different Alfalfa Cultivars under Saline-Alkaline Stress and their Relationship with Soil Environmental Factors

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Phyton-International Journal of Experimental Botany 2024年第1期93卷 29-43页

作者： Tian-Jiao Wei Guang Li Yan-ru Cui Jiao Xie Xing-ai Gao Xing Teng Xin-Ying Zhao Fa-Chun Guan Zheng-Wei Liang Northeast Agricultural Research Center of China Jilin Academy of Agricultural SciencesChangchun130033China Northeast Institute of Geography and Agroecology Chinese Academy of SciencesChangchun130102China

Soil salinization is the main factor that threatens the growth and development of plants and limits the increase of *** is of great significance to study the key soil environmental factors affecting plant root traits to reveal the adaptation strategies of plants to saline-alkaline-stressed soil *** this study,the root biomass,root morphological parameters and root mineral nutrient content of two alfalfa cultivars with different sensitivities to alkaline stress were analyzed with black soil as the control group and the mixed saline-alkaline soil with a ratio of 7:3 between black soil and saline-alkaline soil as the saline-alkaline treatment *** the same time,the correlation analysis of soil salinity indexes,soil nutrient indexes and the activities of key enzymes involved in soil carbon,nitrogen and phosphorus cycles was carried *** results showed that compared with the control group,the pH,EC,and urease(URE)of the soil surrounding the roots of two alfalfa cultivars were significantly increased,while soil total nitrogen(TN),total phosphorus(TP),organic carbon(SOC),andα-glucosidase activity(AGC)were significantly decreased under saline-alkaline *** was no significant difference in root biomass and root morphological parameters of saline-alkaline tolerant cultivar GN under saline-alkaline *** number of root tips(RT),root surface area(RS)and root volume(RV)of AG were reduced by 61.16%,44.54%,and 45.31%,respectively,compared with control *** ratios of K^(+)/Na^(+),Ca^(2+)/Na^(+)and Mg^(2+)/Na^(+)of GN were significantly higher than those of AG(p<0.05).The root fresh weight(RFW)and dry weight(RDW),root length(RL),RV and RT of alfalfa were positively regulated by soil SOC and TN,but negatively regulated by soil pH,EC,and URE(p<0.01).Root Ca^(2+)/Na+ratio was significantly positively correlated with soil TN,TP and SOC(p<0.01).The absorption of Mg and Ca ions in roots is significantly negatively regulated by soilβ-glucosidase ac

关键词： Saline-alkaline stress alfalfa root traits soil environmental factors correlation analysis

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Preliminary research and scheme design of deep underground in situ geo-information detection experiment for Geology in Time

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International Journal of Mining Science and Technology 2024年第1期34卷 1-13页

作者： Heping Xie ru Zhang Zetian Zhang Yinshuang ai Jianhui Deng Yun Chen Yong Zhou Mingchuan Li Liqiang Liu Mingzhong Gao Zeqian Yang Weiqiang Ling Heng Gao Qijun Hao Kun Xiao Chendi Lou State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering Sichuan UniversityChengdu 610065China College of Water Resource&Hydropower Sichuan UniversityChengdu 610065China Guangdong Provincial Key Laboratory of Deep Earth Sciences and Geothermal Energy Exploitation and Utilization Institute of Deep Earth Sciences and Green EnergyShenzhen UniversityShenzhen 518060China Institute of Geology and Geophysics Chinese Academy of SciencesBeijing 100029China Yalong River Hydropower Development Company Limited Chengdu 610051China

The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the Long-Range Objectives Through the Year *** is important to reveal the evolutionary process and mechanism of deep tectonics to understand the earth’s past,present and *** academic con-notation of Geology in Time has been given for the first time,which refers to the multi-field evolution response process of geological bodies at different time and spatial scales caused by geological processes inside and outside the *** on the deep in situ detection space and the unique geological envi-ronment of China Jinping Underground Laboratory,the scientific issue of the correlation mechanism and law between deep internal time-varying and shallow geological response is given *** research and frontier exploration on deep underground in situ geo-information detection experiments for Geology in Time are designed to be carried out,which will have the potential to explore the driving force of Geology in Time,reveal essential laws of deep earth science,and explore innovative technologies in deep underground engineering.

关键词： Deep underground Geology in Time China Jinping Underground Laboratory In situ detection

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Applying Hybrid Clustering in Pulsar Candidate Sifting with Multi-modality for FAST Survey

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Research in Astronomy and Astrophysics 2024年第3期24卷 283-296页

作者： Zi-Yi You Yun-Rong Pan Zhi Ma Li Zhang Shuo Xiao Dan-Dan Zhang Shi-Jun Dang ru-Shuang Zhao Pei Wang ai-Jun Dong Jia-Tao Jiang Ji-Bing Leng Wei-An Li Si-Yao Li School of Physics and Electronic Science Guizhou Normal UniversityGuiyang 550025China Guizhou Provincial Key Laboratory of Radio Astronomy and Data Processing Guizhou Normal UniversityGuiyang 550025China College of Big Data and Information Engineering Guizhou UniversityGuiyang 550025China CAS Key Laboratory of FAST National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China Key Laboratory of Information and Computing Guizhou Province Guizhou Normal UniversityGuiyang 550001China School of Big data and Computer Science Guizhou Normal UniversityGuiyang 550025China Guizhou Software Engineering Research Center Guiyang 550000China

Pulsar search is always the basis of pulsar navigation,gravitational wave detection and other research ***,the volume of pulsar candidates collected by the Five-hundred-meter Aperture Spherical radio Telescope(FAST)shows an explosive growth rate that has brought challenges for its pulsar candidate filtering ***,the multi-view heterogeneous data and class imbalance between true pulsars and non-pulsar candidates have negative effects on traditional single-modal supervised classification *** this study,a multi-modal and semi-supervised learning based on a pulsar candidate sifting algorithm is presented,which adopts a hybrid ensemble clustering scheme of density-based and partition-based methods combined with a feature-level fusion strategy for input data and a data partition strategy for *** on both High Time Resolution Universe SurveyⅡ(HTru2)and actual FAST observation data demonstrate that the proposed algorithm could excellently identify pulsars:On HTru2,the precision and recall rates of its parallel mode reach0.981 and 0.988 *** FAST data,those of its parallel mode reach 0.891 and 0.961,meanwhile,the running time also significantly decreases with the increment of parallel nodes within ***,we can conclude that our algorithm could be a feasible idea for large scale pulsar candidate sifting for FAST drift scan observation.

关键词： methods data analysis-surveys-methods numerical

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Automated and miniaturized screening of antibiotic combinations via robotic-printed combinatorial droplet platform

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1801-1813页

作者： Fangchi Shao Hui Li Kuangwen hsieh Pengfei Zhang Sixuan Li Tza-Huei Wang Department of Biomedical Engineering Johns Hopkins UniversityBaltimoreMD 21218USA Department of Mechanical Engineering Johns Hopkins UniversityBaltimoreMD 21218USA

Antimicrobial resistance(AMR)has become a global health crisis in need of novel *** this end,antibiotic combination therapies,which combine multiple antibiotics for treatment,have at-tracted significant attention as a potential approach for combating *** facilitate advances in anti-biotic combination therapies,most notably in investigating antibiotic interactions and identifying synergistic antibiotic combinations however,there remains a need for automated high-throughput plat-forms that can create and examine antibiotic combinations on-demand,at scale,and with minimal reagent *** address these challenges,we have developed a Robotic-Printed Combinatorial Droplet(RoboDrop)platform by integrating a programmable droplet microfuidic device that generates antibiotic combinations in nanoliter droplets in automation,a robotic arm that arranges the droplets in an array,and a camera that images the array of thousands of droplets in *** further implement a resazurin-based bacterial viability assay to accelerate our antibiotic combination *** a demonstration,we use RoboDrop to corroborate two pairs of antibiotics with known interactions and subsequently identify a new synergistic combination of cefsulodin,penicillin,and oxacillin against a model *** *** therefore envision RoboDrop becoming a useful tool to efficiently identify new synergistic antibiotic combinations toward combating AMR.

关键词： Droplet microfluidics Antimicrobial resistance Antibiotic combinatorial screening Contact printing Robotics Pharmaceutical analysis High-throughput Antibiotic interactions

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Associations of prenatal exposure to bisphenols with BMI growth trajectories in offspring within the first two years:evidence from a birth cohort study in China

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World Journal of Pediatrics 2024年第7期20卷 701-711页

作者： Chao Xiong Kai Chen Lu-Li Xu Yi-Ming Zhang Hua Liu Meng-Lan Guo Zhi-Guo Xia Yu-Ji Wang Xiao-Feng Mu Xiao-Xuan Fan Jing-Quan Chen Yu-ru Liu Yuan-Yuan Li Wei Xia You-Jie Wang ai-Fen Zhou Wuhan Children’s Hospital(Wuhan Maternal and Child Healthcare Hospital) Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430016China Key Laboratory of Environment and Health Ministry of Education and Ministry of Environmental Protectionand State Key Laboratory of Environmental HealthSchool of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Maternal and Child Health School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Background Prenatal bisphenol exposure has been reported to be associated with lower birth weight and obesity-related indicators in early *** findings warrant an investigation of the relationship between prenatal bisphenol exposure and the dynamic growth of *** study aimed to evaluate the relationship of maternal bisphenol concentration in urine with the body mass index(BMI)growth trajectory of children aged up to two years and to identify the critical exposure *** A total of 826 mother–offspring pairs were recruited from Wuhan Children’s Hospital between November 2013 and March *** urine samples collected during the first,second,and third trimesters were analyzed for bisphenol A(BPA),bisphenol S,and bisphenol F(BPF)*** of length and weight were taken at 0,1,3,6,8,12,18,and 24 ***'s BMI was standardized using the World Health Organization reference,and group-based trajectory modeling was used to identify BMI growth *** associations between prenatal bisphenol exposure and BMI growth trajectory patterns were assessed using multinomial logistic regression *** The BMI growth trajectories of the 826 children were categorized into four patterns:low-stable(n=134,16.2%),low-increasing(n=142,17.2%),moderate-stable(n=350,42.4%),and moderate-increasing(n=200,24.2%).After adjusting for potential confounders,we observed that prenatal exposure to BPA during the second trimester[odds ratio(OR)=2.20,95%confidence interval(CI)=1.09–4.43]and BPF during the third trimester(OR=3.28,95%CI=1.55–6.95)at the highest quartile concentration were associated with an increased likelihood of the low-increasing BMI ***,in the subgroup analysis by infant sex,the positive association between the highest quartile of prenatal average urinary BPF concentration during the whole pregnancy and the low-increasing BMI trajectory was found only in girls(OR=2.82,95%CI=1.04–7.68).Conclus

关键词： Birth cohort study Bisphenol A Bisphenol S Bisphenol F Body mass index trajectory Prenatal exposure

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Leaching kinetics and permeability of polyethyleneimine added ammonium sulfate on weathered crust elution-deposited rare earth ores

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Journal of Rare Earths 2024年第8期42卷 1610-1619,I0008页

作者： Huifang Yang Aoyang Sha Zhengyan He Chenjie Wu Yuanlai Xu Jingjing Hu Zhigao Xu ru'an Chi Key Laboratory for Green Chemical Process of Ministry of Education School of Resources&Safety EngineeringWuhan Institute of TechnologyWuhan 430073China Key Laboratory of Resources Conversion and Pollution Control of the State Ethnic Affairs Commission College of Resources and Environmental ScienceSouth-Central MinzuUniversityWuhan430074China

At present,in-situ leaching of weathered crust elution-deposited rare earth ores(WCE-DREOs) encounter with problems such as long leaching cycles,slow infiltration rate and low product *** order to solve the above problems,the conventional leaching agent ammonium sulfate((NH_(4))_(2)SO_(4)) was compounded with polymeric surfactant polyethyleneimine(PEI) to form a composite leaching *** effects of leaching temperature,PEI *** rate and pH on leaching kinetics and permeability of rare earths(RE) and aluminum(ai) in orebody were *** is found that with temperature increasing,the time required to reach leaching equilibrium for both RE and Al is shortened,the apparent activation energies of RE and ai are 14.79 and 13.45 kJ/mol,respectively,and the leaching processes are in accordance with the outer diffusion control,When the concentrations of(NH_(4))_(2)SO_(4) and PEI in the composite leaching agent are 2.0 wt% and 0.4 wt%;the time required to reach leaching equilibrium for RE and ai is about 50% shorter than that using(NH_(4))_(2)SO_(4) alone,and the leaching efficiencies of RE are slightly higher than that of *** increasing the temperature and flow rate of the composite leaching agent can improve the leaching efficiencies of RE and Al,but pH has neglected effects on the leaching efficiencies of *** PEI concentrations below 0,4 wt%, the addition of PEI promotes the leaching of RE and *** column leaching studies of the WCE-DREO,the addition of 0.4 wt% PEI to the traditional leaching agent(NH_(4))_(2)SO_(4) has no impact on the leaching efficiencies of ***,it can significantly increase the infiltration rate of WCE-DREO,shortening the leaching time per 10 mL effluent from about 30 min for(NH_(4))_(2)SO_(4) leaching system to 20 min for the composite leaching *** leaching time is shortened by one-third,and the leaching cost is reduced,which can provide theoretical guidance for the development and commercial implementa

关键词： Polyethyleneimine Kinetics Rare earths Permeability Activation energy

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星斗山天然次生林优势种的生态位和种间联结动态

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林业科学研究 2024年第1期37卷 171-182页

作者：何江徐来仙艾训儒中国林业科学研究院林业科技信息研究所北京100091 湖北民族大学林学园艺学院湖北恩施445000 广西大学林学院广西南宁530004

[目的]常绿落叶阔叶混交林是我国特有的天然林类型,作为亚热带山地的主要植被类型。探讨典型亚热带常绿落叶阔叶混交林优势种的生态位和种间联结动态变化,阐明短期群落发展中优势种的适应能力、种间关系和变化规律,为亚热带地区生物资... 详细信息

[目的]常绿落叶阔叶混交林是我国特有的天然林类型,作为亚热带山地的主要植被类型。探讨典型亚热带常绿落叶阔叶混交林优势种的生态位和种间联结动态变化,阐明短期群落发展中优势种的适应能力、种间关系和变化规律,为亚热带地区生物资源保护、恢复和森林管理提供理论依据。[方法]本研究以湖北星斗山国家级自然保护区天然次生林为研究对象,结合2013年和2018年的两次固定样地(48个)调查监测数据,对比分析重要值排名前10名的优势种(重要值大于2%)的生态位宽度、生态位重叠指数、方差比率法、χ^(2)检验、联结系数、Jaccard指数和Spearman秩相关系数的动态变化。[结果](1)2013年和2018年物种组成变化较小,物种株数、科属种数减少;重要值排名前10名的优势种仅1名物种发生变动。(2)两次调查结果的生态位重叠指数均以0.2~0.5之间为主,种对数随群落发展而增加,大部分物种对生境需求具有一定相似性、互补性。随着群落发展,多数优势种的生态位重叠程度逐渐降低、生态位宽度逐渐变窄、利用资源能力逐渐减弱。(3)5年间,重要值排序与生态位宽度排序并不完全相同,群落资源未被完全充分利用。水榆花楸和翅柃一直占据优势地位,与其他优势种的生态位重叠指数呈现稳定或增加趋势。(4)两次调查中优势种的总体联结性均为显著正关联,χ^(2)检验、联结系数、Jaccard指数和Spearman秩相关系数的结果基本一致:正关联种对数大于负关联种对数,极显著或显著正关联种对数比极显著或显著负关联种对数多,不显著关联种对数也占有重要比例。随着群落发展,总体联结性的显著正关联逐渐增强,正相关对数和正负比值逐渐增加,负相关对数逐渐减少。[结论]湖北星斗山国家级自然保护区天然次生林处于自然演替的早期至中期阶段,种间联结松散、不紧密,水榆花楸和翅柃竞争力强,长蕊杜鹃、多脉青冈和包果柯常绿树种对环境资源利用能力不断提高。

关键词：常绿落叶阔叶混交林星斗山国家级自然保护区鄂西南重要值生态位种间联结性次生林动态

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重金属污染水体背景下的底质反射率光谱特征及其对离水反射率贡献影响分析

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光谱学与光谱分析 2024年第1期44卷 111-117页

作者：梁业恒邓孺孺梁钰婕刘永明吴仪袁宇恒艾先俊中山大学地理科学与规划学院广东厂州510006 广东省水环境遥感监测工程技术研究中心广东广州510006 中国科学院南海海洋研究所热带海洋环境国家重点实验室广东广州510301 香港中文大学地理与资源管理系中国香港990775 北京师范大学地理科学学部北京100875 广州市城市规划勘测设计研究院花都分院广东广州510060

水体重金属遥感反演是水环境遥感领域中的难题,目前仍有相当多的基础性问题有待解决,而浅水区底质对离水反射率贡献规律是将来遥感反演模型精度其中一个重要影响因素,尤其在重金属污染这种特殊的背景下,揭示其贡献规律对提升水体重金属... 详细信息

水体重金属遥感反演是水环境遥感领域中的难题,目前仍有相当多的基础性问题有待解决,而浅水区底质对离水反射率贡献规律是将来遥感反演模型精度其中一个重要影响因素,尤其在重金属污染这种特殊的背景下,揭示其贡献规律对提升水体重金属遥感模型精度有重要作用,测量结果对研究重金属尾矿底泥的反射率光谱特征以及区分常见水底底质有参考意义。首先利用光谱仪测量获得广东大宝山尾矿底质反射率,发现其在波长755、 1 280、 1 620和2 200 nm存在反射峰,有明显光谱特征,并与河床常见的粗沙、淤泥和石头三类底质反射率进行了对比分析,结果表明:一方面,粗沙和淤泥反射率呈一缓慢上升的曲线,与存在多个特征反射峰的矿区底泥有明显区别;另一方面,石头反射率则在波长范围550~650 nm出现一个宽而扁平的反射峰后随即在波长675 nm处出现波谷然后增大至波长750 nm后趋于平缓,其特征波长与矿区底泥均不一样。上述特征波长可作为重金属污染背景下底泥光谱的重要区分波段。测量水深为1 cm、 10 cm和深水区的离水反射率,结合水质遥感模型计算出1 cm水深情况下底质反射光、水体散射光,并将其对离水反射率贡献进行讨论。离水反射率测量结果表明,底质对浅水区影响极大,随着水深变浅,离水反射率总体不断被抬升。而水底反射光和水体散射光对离水反射率贡献规律则以波长515 nm为分界线,向短波方向以水体散射光贡献为主,向长波方向则以水底反射光为主,贡献量由底质反射能力和水体散射能力共同决定。对考虑了底质反射光后的遥感模型精度进行评价,模型计算得到波长范围350~950 nm内的离水反射率与野外测量值对比结果,二者具有显著的线性相关(R^(2)=0.964 2),相对误差在波长范围560~830 nm低于10%,甚者可低于5%,模型总体模拟精度较好,远比在不考虑底质影响时精度高,模型满足将来水中重金属遥感反演要求。研究结果对今后水体重金属遥感反演时处理底质影响提供了重要的参考数据和理论依据,有助推动该领域进一步发展。

关键词：水体重金属遥感重金属矿区底质反射率光谱特征分析模型精度评价

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