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Impact of endoscopically minimal involvement on IL-8 mRNA expression in esophageal mucosa of patients with non-erosive reflux disease

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World Journal of Gastroenterology 2003年第12期9卷 2801-2804页

作者： YuseiKanazawa HajimeIsomoto Chun-YangWen ai-pingwang VladimirASaenko AkiraOhtsuru FuminaoTakeshima KatsuhisaOmagari YoheiMizuta IkuoMurata ShunichiYamashita ShigeruKohno SecondDepartmentofInternalMedicine AtomicBombDiseaseInstituteNagasakiUniversitySchoolofMedicineNagasakiJapan DepartmentofMolecularPathology AtonaicBombDiseaseInstituteNagasakiUniversitySchoolofMedicineNagasakiJapan DepartmentofMolecularMedicine AtomicBombDiseaseInstitute~NagasakiUniversitySchoolofMedicineNagasakiJapan DepartmentofPharmacotherapeutids NagasakiUniversityGraduateSchoolofBiomedicalSciencesNagasakiJapan

aiM:Little has been known about the pathogenesis of non- erosive reflux disease(NERD).Recent studies have implicated interleukin 8(IL-8)in the development and progression of gastroesophgeal reflux disease(GERD).The purpose of this study was to determine IL-8 RNA expression levels in NERD patients with or without subtle mucosal changes. METHODS:We studied 26 patients with NERD and 13 asymptomatic *** sample was taken from the esophagus 3 cm above the gastroesophageal junction and snap frozen for measurement of IL-8 mRNA levels by real-time quantitative polymerase chain reaction(PCR).We also examined mRNA expression of IL-8 receptors,CXCR-1 and -2 by reverse transcriptase *** patients were endoscopically classified into grade M(mucosal color changes without visible mucosal break)and N(neither minimal involvement nor mucosal break)of the modified Los Angeles classification. RESULTS:The relative IL-8 mRNA expression levels were significantly higher in esophageal mucosa of NERD patients than those in esophageal mucosa of the *** was a significant difference in IL-8 mRNA levels between grades M and *** CXCR-1 and -2 mRNAs were constitutively expressed in esophageal ***: Our results suggest that high IL-8 levels in esophageal mucosa may be involved in the pathogenesis of NERD through interaction with its receptors. NERD seems to be composed of a heterogeneous population in terms of not only endoscopically minimal involvement but also immune and inflammatory processes.

关键词： Adult Aged Alcohol Drinking Base Sequence Comparative Study DNA Primers Endoscopy, Digestive System Female Gastroesophageal Reflux Gene Expression Regulation Helicobacter Infections Helicobacter pylori Hernia, Hiatal Humans Interleukin-8 Male Middle Aged RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Risk Factors Smoking

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Expression of pituitary tumor transforming gene in human gastric carcinoma

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World Journal of Gastroenterology 2004年第4期10卷 481-483页

作者： Chun-YangWen ToshiyukiNakayama MutsumiMatsuu KazukoShichijo IchiroSekine ai-pingwang Masahiro Nakashima Yi-Tao Ding Masahiro Ito Hiromi Ishibashi Department of Molecular Pathology Atomic Bomb Disease InstituteNagasaki University Graduate School of Biomedical Sciences1-12-4 SakamotoNagasaki 852-8523Japan Department of Digestive Disease Nanjing Drum Tower HospitalMedical School of Nanjing UniversityNanjing 210008Jiangsu ProvinceChina Tissue and Histopathology Section Division of Scientific Data Registry Atomic Bomb Disease InstituteNagasaki University Graduate School of Biomedical Sciences1-12-4 SakamotoNagasaki 852-8523Japan Department of Hepatobiliary Surgery Nanjing Drum Tower HospitalMedical School of Nanjing UniversityNanjing 210008Jiangsu ProvinceChina Department of Pathology National Nagasaki Medical Center2-1001-1 KubaraOmuraNagasaki 856-8562Japan Clinical Research Center National Nagasaki Medical Center2-1001-1 KubaraOmuraNagasaki 856-8562Japan

aiM:Pituitary tumor transforming gene (PTTG1) is overexpressed in a variety of tumors, including carcinomas of the lung, breast, colon, as well as in leukemia, lymphoma and pituitary ***,there is little information on its expression in gastric carcinoma. We sought to investigate the expression of PTTG1 in gastric carcinoma and to explore the relationship between its expression and clinicopathological ***:We studied 75 primary human gastric adenocarcinomas, including 17 mucosal carcinomas, 21 submucosal infiltrative carcinomas, 12 carcinomas invading proprial muscle layers, 6 carcinomas reaching the subserosa,and 19 carcinomas penetrating the serosal *** analysis was performed using paraffinembedded sections of gastric ***: PTTG1 was expressed heterogeneously in carcinomas. Positive PTTG1 staining was observed in 65.3% of the carcinomas (49 of 75). Its expression did not correlate significantly with either the histological type or the depth of infiltration of the gastric ***,a statistical analysis showed significant differences between the primary adenocarcinomas and the associated metastatic lymph ***: The results of this study demonstrate that PTTG1 expression is enhanced in metastatic lymph nodes in comparison to that in primary carcinomas. We suggest that PTTG1 may contribute to lymph node metastases in gastric carcinoma.

关键词：垂体肿瘤转化基因胃癌基因表达免疫组织化学肿瘤侵袭肿瘤病理学

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Antitumor immunity induced by DNA vaccine encoding alpha-fetoprotein/heat shock protein 70

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World Journal of Gastroenterology 2004年第21期10卷 3197-3200页

作者： Xiao-pingwang Guo-ZhenLiu ai-LiSong Hai-YanLi YuLiu DepartmentofPathology CapitalUniversityofMedicalSciencesBeijing100054China

aiM:To construct a DNA vaccine encoding human alpha-fetoprotein (hAFP)/heat shock protein 70 (HSP70), and to study its ability to induce specific CTL response and its protective effect against AFP-expressing ***: A DNA vaccine was constructed by combiningh AFP gene with HSP70 gene. SP2/0 cells were stably transfected with pBBS212-hAFP and pBBS212-hAFP/HSP70 eukaryotic expression vectors. Mice were primed and boosted with DNA vaccine hAFP/HSP70 by intramuscular injection, whereas plasmid with hAFP or HSP70 was used as controls. ELISPOT and ELISA were used to detect IFN-γ-producing splenocytes and the level of serum anti-AFP antibody from immunized mice respectively. In vivo tumor challenge was measured to assess the immune effect of the DNA ***: By DNA vaccine immunization, the results of ELISPOT and ELISA showed that the number of IFN-γ-producing splenocytes and the level of serum anti-AFP antibody were significantly higher in rhAFP/HSP70 group than in hAFP and empty plasmid groups (95.50±10.90 IFN-γ spots/10^6 cells vs 23.60±11.80 IFN-γ spots/10^6 cells,7.17±4.24 IFN-γ spots/106 cells, P<0.01; 126.50±8.22μg/mL vs 51.72±3.40μg/mL, 5.83±3.79μg/mL, P<0.01). The tumor volume in rhAFP/HSP70 group was significantly smaller than that in pBBS212-hAFP and empty plasmid groups (37.41±7.34 mm^3 vs 381.13±15.48 mm^3, 817.51±16.25 mm^3,P<0.01). CONCLUSION: Sequential immunization with a recombinant DNA vaccine encoding AFP and heat shock protein70 could generate effective AFP-specific T cell responses and induce definite antitumor effects on AFP-producing tumors, which may be suitable for some clinical testing as a vaccine for HCC.

关键词：抗癌作用免疫性 DNA 疫苗编码 α-胎蛋白热损害蛋白70 hAFP

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