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Influence on Biological Behavior of Colon Cancer Stem Cells after RNA Interfering CD133

Clinical oncology and cancer researeh 2010年第6期7卷 359-365页

作者： Liang ai mei liu Mi CHEN Shao-lin LI Teaching and Research Section of Radiation Medicine School of Basic Medical Science Chongqing Medical University Chongqing 400016. China.

OBJECTIVE To observe the effects expression and activation on biological cancer stem cells. of blocking CD133 gene characteristic of the colon METHODS CD133＋ colon cancer stem cells （CCSCs） were separated from EpCAMhigh CD44＋ CCSCs through fluorescenceactivated cell sorting （FACS）. The proliferation, the capability of spherical cell formation, neoplasia, and the expression of ABCG2 mRNA of CD133＋ CCSCs were observed after the CD133＋ CCSCs were infected with LV-CD133shRNA. CD133 negative cells were isolated from EpCAMhigh CD44＋ CCSCs with FACS, and the CD133 proteins in CD133- cells were detected with Western blot. RESULTS CD133＋ CCSCs were isolated from EpCAMhigh CD44＋ CCSCs using FACS, and they accounted for 89.2% in the stem cells. In the experimental group, after the CD133＋ CCSCs were knocked down by LV-CD133shRNA RNai, the growth pattern of the cells in the stem cell culture changed into adherent growth from suspended growth, and couldn＇t generate spherical cells. Results of MTT assay showed that the CD133＋ CCSCs infected with LV-CD133shRNA grew slowly, compared to the cells in the control groups. There was a decrease in the cloning efficiency. The infected cells were transplanted into the BALB/c nude mice. During the observation, no neoplasia was found in the CD133＋ cells infected with LV-CD133shRNA. The level of ABCG2 mRNA expression was lowered greatly （P 〈 0.01）. CD133- cells were obtained from the EpCAMhigh CD44＋ CCSCs using FACS, in which the expression of CD133 protein was positive. CONCLUSION CD133 retains the biological characteristics of the colon cancer stem cells.

关键词： colonic neoplasms stem cells CD133 protein mouse RNA interference biological behavior.

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Human adipose tissue-and umbilical cord-derived stem cells: which is a better alternative to treat spinal cord injury?

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Neural Regeneration Research 2020年第12期15卷 2306-2317页

作者： ai-mei liu Bo-Li Chen Ling-Tai Yu Tao liu Ling-Ling Shi Pan-Pan Yu Yi-Bo Qu Kwok-Fai So Li-Bing Zhou Guangdong-Hongkong-Macao Institute of CNS Regeneration Ministry of Education CNS Regeneration Collaborative Joint LaboratoryJinan UniversityGuangzhouGuangdong ProvinceChina Guangzhou Regenerative Medicine and Health Guangdong Laboratory GuangzhouGuangdong ProvinceChina Co-innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

Multiple types of stem cells have been proposed for the treatment of spinal cord injury,but their comparative information remains *** this study,a rat model of T10 contusion spinal cord injury was established by the impactor *** umbilical cord-derived mesenchymal stem cells(UCMSCs)or human adipose tissue-derived mesenchymal stem cells(ADMSCs)(2.5μL/injection site,1×10^5 cells/μL)was injected on rostral and caudal of the injury segment on the ninth day after *** injected with mesenchymal stem cell culture medium were used as *** results show that although transplanted UCMSCs and ADMSCs failed to differentiate into neurons or glial cells in vivo,both significantly improved motor and sensory *** spinal cord injury,UCMSCs and ADMSCs similarly promoted spinal neuron survival and axonal regeneration,decreased glial scar and lesion cavity formation,and reduced numbers of active *** analysis of spinal samples showed a specific increase of interleukin-10 and decrease of tumor necrosis factorαin the ADMSC group,as well as a downregulation of macrophage inflammatory protein 3αin both UCMSC and ADMSC groups at 3 days after cell *** of interleukin-10 and interleukin-13 was observed in both UCMSC and ADMSC groups at 7 days after cell *** tagging for relative and absolute quantitation proteomics analyses showed that UCMSCs and ADMSCs induced changes of multiple genes related to axonal regeneration,neurotrophy,and cell apoptosis in common and specific *** conclusion,UCMSC and ADMSC transplants yielded quite similar contributions to motor and sensory recovery after spinal cord injury via anti-inflammation and improved axonal ***,there were some differences in cytokine and gene expression induced by these two types of transplanted *** experiments were approved by the Laboratory Animal Ethics Committee at Jinan University(approval No.20180228026)on Febru

关键词： behavior central nervous system factor inflammation model spinal cord stem cells transplantation

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Recent progress in the applications of presynaptic dopaminergic positron emission tomography imaging in parkinsonism

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Neural Regeneration Research 2025年第1期20卷 93-106页

作者： Yujie Yang Xinyi Li Jiaying Lu Jingjie Ge Mingjia Chen Ruixin Yao mei Tian Jian Wang Fengtao liu Chuantao Zuo Key Laboratory of Arrhythmias Ministry of EducationDepartment of Medical GeneticsShanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina Department of Neurology National Research Center for Aging and MedicineNational Center for Neurological Disordersand State Key Laboratory of Medical NeurobiologyHuashan HospitalFudan UniversityShanghaiChina Department of Nuclear Medicine&PET Center National Center for Neurological Disordersand National Clinical Research Center for Aging and MedicineHuashan HospitalFudan UniversityShanghaiChina International Human Phenome Institutes(Shanghai) ShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Nowadays,presynaptic dopaminergic positron emission tomography,which assesses deficiencies in dopamine synthesis,storage,and transport,is widely utilized for early diagnosis and differential diagnosis of *** review provides a comprehensive summary of the latest developments in the application of presynaptic dopaminergic positron emission tomography imaging in disorders that manifest *** conducted a thorough literature search using reputable databases such as PubMed and Web of *** criteria involved identifying peer-reviewed articles published within the last 5 years,with emphasis on their relevance to clinical *** findings from these studies highlight that presynaptic dopaminergic positron emission tomography has demonstrated potential not only in diagnosing and differentiating various Parkinsonian conditions but also in assessing disease severity and predicting ***,when employed in conjunction with other imaging modalities and advanced analytical methods,presynaptic dopaminergic positron emission tomography has been validated as a reliable in vivo *** validation extends to screening and exploring potential neuropathological mechanisms associated with dopaminergic *** summary,the insights gained from interpreting these studies are crucial for enhancing the effectiveness of preclinical investigations and clinical trials,ultimately advancing toward the goals of neuroregeneration in parkinsonian disorders.

关键词： aromatic amino acid decarboxylase brain imaging dopamine transporter Parkinson’s disease parkinsonism positron emission tomography presynaptic dopaminergic function vesicle monoamine transporter type 2

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γ-干扰素释放试验在我国应用的现状与进展

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结核病与肺部健康杂志 2016年第1期5卷 62-68页

作者：刘爱梅刘存旭广西龙潭医院结核科广西结核病防治临床中心柳州545005

γ-干扰素释放分析试验（IGRA）是检测结核分枝杆菌感染的新技术.笔者依据国内临床研究数据,综述IGRA在我国结核病高负担、新生儿普遍接种卡介苗（BCG）的背景下的现状与进展;探讨其临床应用的可行性及价值;提出临床应用范围等相关建议.

关键词：结核皮肤试验 γ-干扰素体外释放试验

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Modular assembly of complex C-alkyl glycoside enabled via cooperative NHC and photoredox catalysis

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Science China Chemistry 2025年

作者： Rui-Qiang Jiao Ming Li Xi Chen mei-Ze Xu Tian Ding Xiao-Ping Gong Zhuo-mei Li Xue-Yuan liu Yong-Min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou University College of Chemistry and Chemical Engineering Northwest Normal University

C-Alkyl glycosides are found in natural products and are indispensable in drug development programs,owing to their diverse range of medicinal ***-component reactions involving glycosyl radical addition are well documented and proved efficient for the C-alkyl glycosides ***,multicomponent reactions for the rapid and controllable construction of structurally diversified C-alkyl glycosides in a single step remains a significant challenge due to the issues associated with balancing reactivity,compatibility,and ***,we report an NHC and photoredox co-catalyzed three-component method to access complex and valuable C-alkyl *** protocol exhibits broad substrate scope and excellent chemoand ***,nine types of sugars are compatible with this *** addition,the generality of this catalytic strategy was further highlighted by the potential for scalable production,the feasibility of further modifications of the products and its successful application in the late-stage functionalization of pharmaceutical skeletons.

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海藻酸钠-高甲氧基果胶复合体系凝胶特性的研究——蔗糖、Ca^(2+)、葡萄糖酸-δ-内酯对复合体系凝胶特性的影响

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食品科技 2003年第8期28卷 66-68,71页

作者：周爱梅刘欣李立虹林洁陈永泉华南农业大学食品学院广州510642

研究了蔗糖、Ca2+、葡萄糖酸-d-内酯对海藻酸钠—高甲氧基果胶复合体系凝胶特性的影响。结果表明:蔗糖、Ca2+对该体系的凝胶特性影响很大,适量蔗糖的添加不仅可增加体系的凝胶强度和持水性,还可增加凝胶的融点;Ca2+的添加可使体系的凝... 详细信息

研究了蔗糖、Ca2+、葡萄糖酸-d-内酯对海藻酸钠—高甲氧基果胶复合体系凝胶特性的影响。结果表明:蔗糖、Ca2+对该体系的凝胶特性影响很大,适量蔗糖的添加不仅可增加体系的凝胶强度和持水性,还可增加凝胶的融点;Ca2+的添加可使体系的凝胶类型从热可逆变成热不可逆,且对体系凝胶强度的影响与其对海藻酸钠的影响类似;在海藻酸钠、高甲氧基果胶单独不能形成凝胶的条件下,葡萄糖酸-d-内酯的添加可诱导两种胶的混合物形成凝胶,且该复合物的凝胶强度与葡萄糖酸-d-内酯的浓度、胶体总浓度有关。

关键词：海藻酸钠高甲氧基果胶凝胶特性葡萄糖酸—δ—内酯

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Hybrid genetic algorithm for a type-Ⅱ robust mixed-model assembly line balancing problem with interval task times

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Advances in Manufacturing 2019年第2期7卷 117-132页

作者： Jia-Hua Zhang ai-Ping Li Xue-mei liu School of Mechanical Engineering Tongji University Shanghai 201804 People's Republic of China Department of Mechatronics Engineering Wuxi Vocational Institute of Arts and Technology Yixing 214206 Jiangsu People's Republic of China

The typemixed-model assembly line balancing problem with uncertain task times is a critical problem. This paper addresses this issue of practical significance to production efficiency. Herein, a robust optimization model for this problem is formulated to hedge against uncertainty. Moreover, the counterpart of the robust optimization model is developed by duality. A hybrid genetic algorithm (HGA) is proposed to solve this problem. In this algorithm, a heuristic method is utilized to seed the initial population. In addition, an adaptive local search procedure and a discrete Levy flight are hybridized with the genetic algorithm (GA) to enhance the performance of the algorithm. The effectiveness of the HGA is tested on a set of benchmark instances. Furthermore, the effect of uncertainty parameters on production efficiency is also investigated.

关键词： Mixed-model assembly line Assembly line balancing Robust optimization Genetic algorithm (GA) Uncertainty

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工程教育理念下实践教学体系建设的思考

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广东石油化工学院学报 2011年第5期21卷 29-32页

作者：王涛王爱国刘美广东石油化工学院计算机与电子信息学院广东茂名525000

"卓越工程师培养计划"是教育部大力推进的高等工程教育改革的重大措施。分析了高等工程教育目前存在的主要问题,介绍了卓越工程师需具备的能力素质,提出了工程教育中实践教学体系建设的思路及构想,以期对卓越工程师教育改革有所帮助。

关键词：工程教育实践教学卓越工程师培养计划

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Mapping Properties of Certain Oscillatory Integrals on Modulation Spaces

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Acta Mathematica Sinica,English Series 2017年第12期33卷 1647-1658页

作者： mei Fang CHENG Hui Hui liu ai Wen SUN School of Mathematical and Computer Sciences Anhui Normal University Wuhu 241002 P. R. China

Suppose β1 〉 α1 ≥ 0, β2 〉 α2 ≥ 0 and （k,j） ∈ R^2. In this paper, we mainly investigate the mapping properties of the operator Tα,βf（x,y,z）=∫Q^2f（x-t,y-s,z-t^ks^j）e^-2πit-β1s-β2t^-1-α1s^-1-α2 dtd... 详细信息

关键词： Modulation space singular integral operator Wiener amalgam space

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Ordering Trees by Their Spectral Radii

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Acta Mathematicae Applicatae Sinica 2014年第4期30卷 1107-1112页

作者： ai-mei YU Department of Mathematics Beijing Jiaotong University

Let T be a tree with n vertices and let A（T） be the adjacency matrix of T. Spectral radius of T is the largest eigenvalue of A（T）. Wu et al. [Wu, B.F., Yuan, X.Y, and Xiao, E.L. On the spectral radii of trees, Journal of East China Normal University （Natural Science）, 3：22-28 （2004）] determined the first seven trees of order n with the smallest spectral radius. In this paper, we extend this ordering by determining the trees with the eighth to the tenth smallest spectral radius among all trees with n vertices.

关键词： tree spectral radius ordering

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