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Neopestalotiopsis eucalypti,a causal agent of grapevine shoot rot in cutting nurseries in China

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Journal of Integrative Agriculture 2022年第12期21卷 3684-3691页

作者： MA Xuan-yan JIAO Wei-qi LI Heng zhang Wei REN Wei-chao WU Yan zhang Zhi-chang LI Bao-hua ZHOU Shan-yue College of Plant Health and Medicine Qingdao Agricultural University/Engineering Research Center for Precision Pest Management for Fruits and Vegetables of QingdaoQingdao 266109P.R.China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China Shandong Zhichang Agricultural Science and Technology Development Co. Ltd.Rizhao 276500P.R.China

Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total ***,diseases that cause the death of grapevine shoots pose a severe threat to the production of *** this study,the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic *** phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer(ITS)region of ribosomal DNA,part of the translation elongation factor 1-alpha(Tef)and theβ-tubulin(Tub2)*** three isolates were all identified as *** tests of the three fungal isolates were conducted on grapevines shoots in vitro and in *** results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots,and *** was re-isolated from the inoculated grapevine ***,*** was confirmed as a causal agent of the grapevine shoot *** is the first report of *** causing grapevine shoot disease in China.

关键词： Neopestalotiopsis eucalypti grapevine shoot rot multigene phylogenetic analysis pathogenicity

The essential role of N6-methyladenosine RNA methylation in complex eye diseases

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Genes & Diseases 2023年第2期10卷 505-520页

作者： Xiaohua Li Binyun Ma Wenfang zhang Zongming Song Xiaodan zhang Mengyu Liao Xue Li Xueru Zhao Mei Du Jinguo Yu Shikun He hua Yan Henan Provincial People’s Hospital Henan Eye HospitalHenan Eye InstituteHenan Key Laboratory of Ophthalmology and Visual SciencePeople’s Hospital of Zhengzhou UniversityPeople’s Hospital of Henan UniversityZhengzhouHenan 450003China Department of Medicine/Hematology Keck School of Medicine of the University of Southern CaliforniaLos AngelesCA 90033USA Department of Ophthalmology Lanzhou University Second HospitalLanzhouGansu 730030China Department of Ophthalmology Tianjin Medical University General HospitalLaboratory of Molecular OphthalmologyTianjin Medical University. Department of Pharmacology and Tianjin Key Laboratory of Inflammation BiologySchool of Basic Medical SciencesTianjin Medical UniversityTianjin 300052China Department of Pathology and Ophthalmology USC Roski Eye InstituteKeck School of MedicineUniversity of Southern CaliforniaLos AngelesCA 90033USA

There are many complex eye diseases which are the leading causes of blindness,however,the pathogenesis of the complex eye diseases is not fully understood,especially the underlying molecular mechanisms of N6-methyladenosine(m6A)RNA methylation in the eye diseases have not been extensive *** review summarizes the latest advances in the studies of m6A modification in the pathogenesis of the complex eye diseases,including cornea disease,cataract,diabetic retinopathy,age-related macular degeneration,proliferative vitreoretinopathy,Graves’disease,uveal melanoma,retinoblastoma,and traumatic optic *** further discuss the possibility of developing m6A modification signatures as biomarkers for the diagnosis of the eye diseases,as well as potential therapeutic approaches.

关键词： Degeneration Eye diseases Fibrosis Inflammation m6A RNA methylation Tumor

GREPore-seq: A Robust Workflow to Detect Changes After Gene Editing Through Long-range PCR and Nanopore Sequencing

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Genomics, Proteomics & Bioinformatics 2023年第6期21卷 1221-1236页

作者： Zi-Jun Quan Si-Ang Li Zhi-Xue Yang Juan-Juan Zhao Guo-hua Li Feng zhang Wei Wen Tao Cheng Xiao-Bing zhang State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesHaihe Laboratory of Cell EcosystemInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China Center for Stem Cell Medicine Chinese Academy of Medical SciencesTianjin 300020China Department of Stem Cell&Regenerative Medicine Peking Union Medical CollegeTianjin 300020China

To achieve the enormous potential of gene-editing technology in clinical therapies,one needs to evaluate both the on-target efficiency and unintended editing consequences ***,there is a lack of a pipelined,large-scale,and economical workflow for detecting genome editing outcomes,in particular insertion or deletion of a large ***,we describe an approach for efficient and accurate detection of multiple genetic changes after CRISPR/Cas9 editing by pooled nanopore sequencing of barcoded long-range PCR *** the high error rates of Oxford nanopore sequencing,we developed a novel pipeline to capture the barcoded sequences by grepping reads of nanopore amplicon sequencing(GREPore-seq).GREPore-seq can assess nonhomologous end-joining(NHEJ)-mediated double-stranded oligodeoxynucleotide(dsODN)insertions with comparable accuracy to Illumina next-generation sequencing(NGS).GREPore-seq also reveals a full spectrum of homology-directed repair(HDR)-mediated large gene knock-in,correlating well with the fluorescence-activated cell sorting(FACS)analysis *** note,we discovered low-level fragmented and full-length plasmid backbone insertion at the CRISPR cutting ***,we have established a practical workflow to evaluate various genetic changes,including quantifying insertions of short dsODNs,knock-ins of long pieces,plasmid insertions,and large fragment deletions after CRISPR/Cas9-mediated ***-seq is freely available at GitHub(https://***/lisiang/GREPore-seq)and the National Genomics Data Center(NGDC)BioCode(https://***/biocode/tools/BT007293).

关键词： CRISPR/Cas9 Genetic change Long-range PCR Nanopore sequencing GREPore-seq

絮状玄武岩纤维沥青混合料技术研究及工程应用

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石油沥青 2024年第2期38卷 48-52页

作者：郑炳锋张严童浩林峰华春丽朱雨郭崇苏交科集团股份有限公司南京211100 江苏省高速公路经营管理中心南京210009 新型道路材料国家工程研究中心南京211100 东南大学南京211189

絮状玄武岩纤维作为一种高性能的新型矿物纤维,与沥青混合料具有很高的相容性。为研究絮状玄武岩纤维对沥青混合料路用性能的改善效果,首先分析了絮状玄武岩纤维的技术指标,其次开展絮状玄武岩纤维沥青混合料的配合比试验,最后评价了絮... 详细信息

絮状玄武岩纤维作为一种高性能的新型矿物纤维,与沥青混合料具有很高的相容性。为研究絮状玄武岩纤维对沥青混合料路用性能的改善效果,首先分析了絮状玄武岩纤维的技术指标,其次开展絮状玄武岩纤维沥青混合料的配合比试验,最后评价了絮状玄武岩纤维沥青混合料试验路的铺筑效果。结果表明,絮状玄武岩纤维沥青混合料的体积参数满足技术要求,具有优异的高温稳定性,依托宁连一级公路养护工程,铺筑的絮状玄武岩纤维SMA-13试验路取得了较好的应用效果。

关键词：絮状玄武岩纤维沥青混合料配合比设计路用性能工程应用

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马铃薯精量排种技术发展现状与展望

农业装备与车辆工程 2024年第1期62卷 29-33页

作者：李佩文田斌孙伟张华刘小龙李辉甘肃农业大学机电工程学院甘肃兰州730070

中国是马铃薯生产大国,马铃薯种植规模大、范围广。马铃薯种植存在过程繁杂、劳动强度大、效率低等问题,研究马铃薯机械化种植十分重要。目前有多种排种技术应用于马铃薯机械化种植,勺链式排种器结构简单,传动稳定;气吸式排种器能实现... 详细信息

中国是马铃薯生产大国,马铃薯种植规模大、范围广。马铃薯种植存在过程繁杂、劳动强度大、效率低等问题,研究马铃薯机械化种植十分重要。目前有多种排种技术应用于马铃薯机械化种植,勺链式排种器结构简单,传动稳定;气吸式排种器能实现精密排种;针刺式排种器对种薯的适应性较好。针对机械化排种过程中的漏播问题,漏播检测与自动补偿技术对于提高马铃薯播种效率具有重要意义。分析了国内外马铃薯精量排种技术的研究现状,比较不同排种器之间的区别,阐述现阶段马铃薯精量排种存在漏播、消耗功率大、对种薯的损伤较大等问题,为马铃薯精量排种技术的研究提供参考。

关键词：马铃薯排种技术漏播检测自动补偿

Cyclopeptidic Constituents from Arenaria oreophila J.D,Hooker(Caryophyllaceae)

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Journal of Integrative Plant Biology 2006年第6期48卷 740-744页

作者： ai-Qun Jia Ning-hua Tan Jun Zhou State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of Botany the Chinese Academy of Sciences Kunming 650204 Yunnan China

To further investigate the cyclopeptldes of the Caryophyllaceae family, two new cyclopeptldes, named Arenarlphilin E （compound 1） and Arenariphilin F （compound 2）, were obtained from Arenaria oreophUe J. D. Hooker using some Isolation methods, e. g. normal and reverse silica gel. By detailed spectroscopic analysis, such as FAB^＋-MS, 1 D NMR, 2D NMR, the structures of Arenariphilin E （compound 1） and Arenariphilln F （compound 2） were determined as cyclo（lle^1-Gly-Val^1-Ala-Leu-lle^3-lle^2-Val^2-Pro） and cyclo（Pro^2-Pro^1-Gly^2-lle-Val-Leu-Gly^1-aiaThr- Gly^3）, respectively.

关键词： Arenaria oreophila Arenariphilin E Arenariphilin F Caryophyllaceae Cyclopeptide.

凉山彝族地区儿童紫癜性肾炎临床病理特点及预后分析

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西部医学 2024年第6期36卷 895-898,905页

作者：李群石艳花媛媛黄兴琼周继敏张月凉山彝族自治州第一人民医院儿科四川西昌615000

目的探讨凉山彝族地区儿童紫癜性肾的临床病理特点及预后。方法收集2014年1月—2019年12月我院诊断为紫癜性肾炎的患儿383例的临床资料,完善肾组织活检的患儿52例,将临床、病理资料进行回顾性分析,总结凉山彝族地区儿童紫癜性肾炎的年... 详细信息

目的探讨凉山彝族地区儿童紫癜性肾的临床病理特点及预后。方法收集2014年1月—2019年12月我院诊断为紫癜性肾炎的患儿383例的临床资料,完善肾组织活检的患儿52例,将临床、病理资料进行回顾性分析,总结凉山彝族地区儿童紫癜性肾炎的年龄、性别、民族分布情况、临床病理特点及预后。结果383例紫癜性肾炎患儿中彝族发病高于汉族(彝∶汉=1.87∶1),彝族女性儿童发病高于彝族男性儿童(彝族女∶彝族男=1.25∶1),汉族女性儿童发病低于汉族男性儿童(汉族女∶汉族男=0.87∶1)。从发病到尿检异常时间看,70.3%肾损害发生在1月内,临床分型主要以血尿和蛋白尿型为主,等级间差异有统计学意义(Z=1.982,P=0.042),其次是肾病综合征型,病理分级以Ⅱ、Ⅲ级为主,占88.4%,在病理分级Ⅱ、Ⅲa级中也以血尿和蛋白尿型为主,病理Ⅲb级以上以肾病综合征型为主,临床分型与病理分级关系各组之间比较差异有统计学意义(均P<0.05),但尤以肾病综合征型差异有显著意义(Z=2.626,P<0.001);尿蛋白定量越高(P<0.05),病理分级越高。汉族和彝族在不同病理分级的比较差异有统计学意义(Z=2.044,P=0.041),彝族儿童肾脏损害程度显得更重;而不同性别在病理分级的比较差异无统计学意义(P≥0.05)。激素、免疫抑制剂、ACEI等治疗效果良好。结论凉山地区儿童紫癜性肾炎以彝族女性儿童发病率更高。同时病理分级与尿蛋白定量呈正相关,且病理分级越高,临床分型以肾病型为主,但也存在病理分级与尿蛋白定量、临床分型不一致表现,提示早期肾活检的重要性;激素和免疫抑制剂治疗效果肯定,预后大多良好。

关键词：彝族儿童紫癜性肾炎病理特点预后

Phytohormone dynamics in developing endosperm influence rice grain shape and quality

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Journal of Integrative Plant Biology 2020年第10期62卷 1625-1637页

作者： Xiao-Fan zhang Jian-hua Tong ai-Ning Bai Chun-Ming Liu Lang-Tao Xiao Hong-Wei Xue School of Life Science and Technology Shanghai Tech UniversityShanghai 201210China Hunan Provincial Key Laboratory of Phytohormones and Growth Development Hunan Agricultural UniversityChangsha 410128China Key Laboratory of Plant Molecular Physiology Institute of Botanythe Chinese Academy of SciencesBeijing 100093China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Joint Center for Single Cell Biology/School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and Ecologythe Chinese Academy of SciencesShanghai 200032China

Hormones are important signaling molecules regulating developmental processes and responses to environmental stimuli in higher *** endosperm,the portion of the seed surrounding the embryo,is the main determinant of rice grain shape and yield;how-ever,the dynamics and exact functions of phyto-hormones in developing endosperm remain *** a systemic study including transcriptome analysis,hormone measurement,and transgene-based endosperm-specific expression of phytohormone bio-synthetic enzymes,we demonstrated that dynamic phytohormone levels play crucial roles in the developing rice endosperm,particularly in regard to grain shape and *** detected diverse,differential,and dra-matically changing expression patterns of genes related to hormone biosynthesis and signaling during endosperm development,especially at early devel-opmental *** chromatography measure-ments confirmed the dynamic accumulation of hor-mones in developing *** transgenic analysis performed on plants expressing hormone bio-synthesis genes driven by an endosperm-specific pro-moter revealed differential effects of the hormones,especially auxin and brassinosteroids,in regulating grain shape and *** studies help elucidate the dis-tinct roles of hormones in developing endosperm and provide novel and useful tools for influencing crop seed shape and yield.

关键词： fir grain shape

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矿山地质环境灾变与保护修复研究现状与展望

金属矿山 2024年第3期 1-18页

作者：于洋陈炳乾花奋奋康建荣江苏师范大学地理测绘与城乡规划学院江苏徐州221116

矿山开采不仅会引发地表沉降、建(构)筑物损毁等安全灾害,还会造成土壤环境破坏、生态系统退化等生态环境灾害。地质环境灾害的类型和特征与采矿方法有关,并且多种地质环境灾害的发生具有耦合性。我国常采用自由垮落、支护加固和剥离排... 详细信息

矿山开采不仅会引发地表沉降、建(构)筑物损毁等安全灾害,还会造成土壤环境破坏、生态系统退化等生态环境灾害。地质环境灾害的类型和特征与采矿方法有关,并且多种地质环境灾害的发生具有耦合性。我国常采用自由垮落、支护加固和剥离排土3种方法处理采区覆岩,分别形成了以长壁垮落法开采、柱式开采和露天开采为代表的3类采空区。综述了3类采区的地质灾害致灾机理、灾害链网形成机制,讨论了地质灾害监测、多灾耦合研究中存在的问题。研究表明:①矿区地质环境灾害主要包括工程安全灾害和生态环境灾害两大类,前者表现为地物空间位置改变引起的采动附加应力损坏各类工程体,后者反映为地物属性变化引起的生态要素缺失和物质能量流动改变;②不同地质环境灾害之间存在耦合性,一种地质环境灾害的出现可能引起一系列次生灾害,形成灾害链网,虽然变形监测、遥感等技术可用于获取地物位置、属性信息,但由于地质环境灾害成灾的复杂性以及传统单一监测预测技术手段的局限性,目前难以对地质灾害进行准确预报预测;③应整合已有的各类变形监测、遥感、传感器、工程检测等技术构建“空—天—建—地—井—人”监测技术体系,融合多源地质环境数据探明矿区物理环境场。在上述分析的基础上,基于多学科交叉视角对矿区地质环境灾害的未来研究方向进行了展望。认为后续研究重点在于:①完善多源数据标准化工作,加强数据安全共享,构建地质环境保护“知识库”;②依托ai辅助和地质环境保护“知识库”,研究矿区多灾耦合机理,明确致灾关键因素,构建灾害链网;③基于灾害链网加强跨学科研究,融合多源数据—模型形成“透明地质条件—矿山开采设计—采动损害/物理场分布特征评价—工程安全预测评价/生态过程预测评价—工程加固维修/生态环境修复”全过程工作机制。分析结果有助于进一步梳理矿区多灾耦合问题的研究思路,对于地质环境灾害综合保护和治理具有较好的参考意义。

关键词：地质环境灾害长壁开采柱式开采露天开采生态修复绿色矿山多学科交叉

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