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Quick recovery and characterization of cell-free DNA in seminal plasma of normozoospermia and azoospermia： implications for non-invasive genetic utilities

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Asian Journal of Andrology 2009年第6期11卷 703-709页

作者： Hong-Gang Li Shi-Yun huang Hui Zhou ai-hua liao Cheng-Liang Xiong Family Planning Research Institute and Centre for Reproductive Medicine Tongji Medical College Huazhong Universityof Science and Technology Wuhan 430030 China

We established a quick and reliable method for recovering cell-free seminal DNA （cfsDNA）, by using the binding-washing-elution procedure on the DNA purification column. Low variations （below 15%） among the triplicate values of cfsDNA quantity verified the reproducibility of our cfsDNA recovery method. Similar cfsDNA yield and size distribution between seminal plasma acquired by filtration and centrifugation confirmed the presence of cfsDNA. To investigate the general characterization of cfsDNA, the quantitation and size distribution of cfsDNA from normozoospermic and azoospermic semen were analyzed by real-time PCR and electrophoresis, respectively. CfsDNA concentration in semen with normozoospermia （n = 11） was 1.34 ± 0.65 μg ·mL^-1, whereas a higher cfsDNA concentration was observed in azoospermia （2.56 ± 1.43 μg ·mL^-1, n = 9）. The continuous distribution of DNA fragments ranging from -1 kb to 15 kb and a spectrum of multiples of 180-bp fragments were observed in each normozoospermic and azoospermic sample. Distinct characteristic DNA ladder fragmentations in some azoospermic samples implicated that cfsDNA originate partly from apoptotic cells. CfsDNAs of 36 selected azoospermic patients with known information of Y chromosome microdeletion were subjected to the same microdeletion analysis by multiplex PCR and PCR amplification of sY114 （1 450 bp）. All multiplex PCR reactions with cfsDNA amplified successfully and provided the same result as leukocyte DNA. PCR amplification of sY114 gave a 1 450-bp amplicon as expected. Our data suggested the potential use of cfsDNA in search of biomarker or diagnostic procedures.

关键词： azoospermia cell-free DNA normozoospermia seminal plasma Y chromosome microdeletion

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The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa

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Asian Journal of Andrology 2006年第3期8卷 301-306,386页

作者： Hong-Gang Li ai-hua liao Xiao-Fang Ding Hui Zhou Cheng-Liang Xiong Centre of Reproductive Medicine.Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030China

aim:To investigate the distribution of cation channel of sperm 1(CATSPER1)protein and the presence of CATSPER1 mRNA in human testis and ejaculated *** influence of anti-human CATSPER1 antibody upon human sperm motility was used to evaluate the function of human CATSPER1 and to estimate its possible use as a target for ***:Human ejaculated sperm from normozoospermic donors(n=12)and liquid nitro- gen frozen human testis were used for the study of mRNA and protein expression of CATSPER1 by reverse transcliption polymerase chain reaction(RT-PCR)and immunohistochemistry,*** from nonnozoospermic donors(n=12)were individually processed using a swim-up procedure and were then incubated with CATSPER 1 antibody at final concentrations of 20,4 and 0.8μg/*** 1,2 and 6h incubation,progressive motility and fast progressive motility were measured by means of computer-assisted semen ***:CATSPER1 transcript was detected in both human testis and each human ejaculated semen ***1 protein expressed in the membrane of spermatid and was localized in the principal piece of the sperm *** application of CATSPER1 antibody at all concentrations significantly inhibited both progressive motility and fast progressive motility after 1,2 and 6h incubation,and significant dose-dependent changes were ***:CATSPER1 is meiotically and post-meiotically expressed in human testis ***1 mRNA in human ejaculated spermatozoa could be a more feasible target for study and infertility screening than testis *** addition,our results suggest that human CATSPER1 could be a possible target for immunocontraception.(Asian J Androl 2006 May:8:301-306)

关键词： (?)ation channel of sperm 1 testis spermatozoa motility immunocontraception human

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18例染色体异常核型女性供精人工授精结局分析

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生殖与避孕 2010年第10期30卷 713-717页

作者：相文佩庞文娟刘丽君李红钢胡廉廖爱华华中科技大学同济医学院计划生育研究所/生殖医学中心武汉430030

目的:探讨染色体核型异常对人工授精妊娠结局的影响。方法:回顾性分析因其配偶为特发性不育而接受供精人工授精(aiD)治疗的1 300例正常女性,外周血淋巴细胞染色体G显带核型异常与供精人工授精治疗结局的关系。结果:检出异常核型18例(染... 详细信息

目的:探讨染色体核型异常对人工授精妊娠结局的影响。方法:回顾性分析因其配偶为特发性不育而接受供精人工授精(aiD)治疗的1 300例正常女性,外周血淋巴细胞染色体G显带核型异常与供精人工授精治疗结局的关系。结果:检出异常核型18例(染色体异常发生率1.38%),包括平衡易位和罗伯逊易位各1例(0.08%),9号染色体臂间倒位9例(0.69%),其他7例(0.54%)。18例染色体核型异常患者术前检查显示其排卵正常,输卵管通畅,子宫及双侧附件均未见明显异常。aiD治疗结果8例未孕,其中罗伯逊易位1例、臂间倒位3例、其他4例,10例妊娠成功。结论:因男性特发性不育患者,特别是进行辅助生殖治疗多个周期后仍未受孕的夫妇,对女方进行细胞遗传学检查是非常必要的。

关键词：染色体核型异常供精人工授精臂间倒位

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男性不育患者精液细菌学分析

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生殖与避孕 2010年第1期30卷 55-58页

作者：周慧刘燕婕杜庆玲王勇段卓廖爱华熊承良华中科技大学同济医学院计划生育研究所/生殖医学中心武汉430030 华中科技大学同济医学院附属协和医院武汉430022

目的:探讨病原体感染男性生殖道后对精液质量的影响及其与男性不育的关系。方法:对2298例不育男性患者的精液进行细菌培养,采用法国梅里埃公司的微量生化反应系统进行细菌鉴定,采用K-B纸片扩散法进行药敏试验,采用MAR法检测抗精子抗体... 详细信息

目的:探讨病原体感染男性生殖道后对精液质量的影响及其与男性不育的关系。方法:对2298例不育男性患者的精液进行细菌培养,采用法国梅里埃公司的微量生化反应系统进行细菌鉴定,采用K-B纸片扩散法进行药敏试验,采用MAR法检测抗精子抗体。结果:2298例标本中208份分离出细菌208株,总检出率为9.05%,其中G+菌188株,占90.38%,G-菌20株,占9.62%,主要优势菌株为金黄色葡萄球菌、溶血葡萄球菌、表皮葡萄球菌;药敏试验显示,以头孢呋辛、头孢唑啉、万古霉素抑菌最为显著;病原体感染不育组的精子活率、活力明显低于未感染的正常对照组,其差异有统计学意义(P<0.05;P<0.01),而在抗精子抗体的检查中,病原体感染不育组的阳性率显著高于对照组。结论:在男性不育的诊治过程中,精液的细菌培养是非常必要的。

关键词：男性不育细菌培养药敏纸片药敏试验

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Structural Modal Parameter Recognition and Related Damage Identification Methods under Environmental Excitations: A Review

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Structural Durability & Health Monitoring 2025年第1期19卷 25-54页

作者： Chao Zhang Shang-Xi Lai hua-Ping Wang School of Civil Engineering and Mechanics Lanzhou UniversityLanzhou730000China Key Laboratory of Special Functional Materials and Structural Design Ministry of EducationLanzhou UniversityLanzhou730000China

Modal parameters can accurately characterize the structural dynamic properties and assess the physical state of the ***,it is particularly signiﬁcant to identify the structural modal parameters according to the monitoring data information in the structural health monitoring(SHM)system,so as to provide a scientiﬁc basis for structural damage identiﬁcation and dynamic model modiﬁ*** view of this,this paper reviews methods for identifying structural modal parameters under environmental excitation and brieﬂy describes how to identify structural damages based on the derived modal *** paper primarily introduces data-driven modal parameter recognition methods(e.g.,time-domain,frequency-domain,and time-frequency-domain methods,etc.),brieﬂy describes damage identiﬁcation methods based on the variations of modal parameters(e.g.,natural frequency,modal shapes,and curvature modal shapes,etc.)and modal validation methods(e.g.,Stability Diagram and Modal Assurance Criterion,etc.).The current status of the application of artiﬁcial intelligence(ai)methods in the direction of modal parameter recognition and damage identiﬁcation is further *** on the pre-vious analysis,the main development trends of structural modal parameter recognition and damage identiﬁcation methods are given to provide scientiﬁc references for the optimized design and functional upgrading of SHM systems.

关键词： Structural health monitoring data information modal parameters damage identiﬁcation ai method

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单层Janus Ga_(2)SSe光催化水解性质的第一性原理研究

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原子与分子物理学报 2025年第3期42卷 45-51页

作者：胡新华廖杨芳贵州省贵阳市乌当区第四中学贵阳550018 贵州师范大学物理与电子科学学院贵阳550025

光催化水解制氢为解决能源危机和环境污染问题提供了一种可行的策略.本文通过第一性原理计算,提出了单层Janus Ga_(2)SSe是一种有效的水解光催化剂.结构稳定性分析表明,单层Janus Ga_(2)SSe具有能量和动力学稳定性.电子性质表明,单层Jan... 详细信息

光催化水解制氢为解决能源危机和环境污染问题提供了一种可行的策略.本文通过第一性原理计算,提出了单层Janus Ga_(2)SSe是一种有效的水解光催化剂.结构稳定性分析表明,单层Janus Ga_(2)SSe具有能量和动力学稳定性.电子性质表明,单层Janus Ga_(2)SSe属于间接带隙半导体,有利于降低载流子的复合几率.光催化性能分析表明,单层Janus Ga_(2)SSe的能带边缘位置适合PH=0~14宽范围的光催化水解.此外,单层Janus Ga_(2)SSe具有较高的紫外光和可见光吸收、较大的内建电场、较小的载流子有效质量保证了光生载流子有效分离并快速迁移到反应活性位点参与水的氧化还原反应.研究结果为单层Janus Ga_(2)SSe的合成及其作为水解光催化剂的潜在应用提供了有价值的理论指导.

关键词： Janus材料光催化水解第一性原理

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Toward High Performance Ambipolar Transport from Super-exchange Perspective:Theoretical Insights for IID-based Copolymers

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Chinese Journal of Polymer Science 2022年第4期40卷 355-364页

作者： Wei-Na Zhang Xiao-Qian Wu Guo Wang Yu-ai Duan hua Geng Yi liao Department of Chemistry Beijing Advanced Innovation Center for Imaging Theory and TechnologyCapital Normal UniversityBeijing 100048China

High-performance ambipolar charge transport materials can reduce the manufacturing cost of OFET and OPV devices,and simplify circuit design and device *** order to obtain ambipolar donor-acceptor(D-A)polymer,many efforts have been made through different donor and acceptor combination,halogenation or heteroatom ***,the influencing factor for charge transport polarity is still much *** on intra-chain super-exchange mechanism for D-A polymer,we found that the energy alignment of donor and acceptor moiety has large impact on charge transport *** the HOMO-LUMO(H-L)gap of the acceptor moiety is narrow,its HOMO/LUMO energy level both lie between the HOMO and LUMO of the donor moiety(sandwich-type energy alignment),and the corresponding D-A copolymers will be more likely ambipolar *** thus,take a narrow H-L gap thiazoleisoindigo(TzIID)acceptor as an example,we demonstrated that a series of TzIID based copolymers combined with wide H-L gap donor moieties can reveal ambipolar *** further predict several high performance ambipolar D-A copolymers(TzIID-TT etc.)with balanced electron and hole transport,whose effective mass(m_(e)^(*)=0.146 and m_(h)^(*)=0.128)is one of the smallest effective masses among ambipolar materials.

关键词： D-A copolymer Ambipolar Super-exchange mechanism Charge transport

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Mini-invasive vs open resection of colorectal cancer and liver metastases: A meta-analysis

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World Journal of Gastroenterology 2019年第22期25卷 2819-2832页

作者： Shan-Ping Ye hua Qiu Shi-Jun liao Jun-hua ai Jun Shi Department of General Surgery First Affiliated Hospital of Nanchang University

BACKGROUND The safety and feasibility of the simultaneous resection of primary colorectal cancer (CRC) and synchronous colorectal liver metastases (SCRLM) have been demonstrated in some studies. Combined resection is expected to be the optimal strategy for patients with CRC and SCRLM. However, traditional laparotomy is traumatic, and the treatment outcome of minimally invasive surgery (MIS) is still obscure. aiM To compare the treatment outcomes of MIS and open surgery (OS) for the simultaneous resection of CRC and SCRLM. METHODS A systematic search through December 22, 2018 was conducted in electronic databases (PubMed, EMBASE, Web of Science, and Cochrane Library). All studies comparing the clinical outcomes of MIS and OS for patients with CRC and SCRLM were included by eligibility criteria. The meta-analysis was performed using Review Manager Software. The quality of the pooled study was assessed using the Newcastle-Ottawa scale. The publication bias was evaluated by a funnel plot and the Begg’s and Egger’s tests. Fixed- and random-effects models were applied according to heterogeneity. RESULTS Ten retrospective cohort studies involving 502 patients (216 patients in the MIS group and 286 patients in the OS group) were included in this study. MIS was associated with less intraoperative blood loss [weighted mean difference (WMD)=-130.09, 95% confidence interval (CI):-210.95 to -49.23, P = 0.002] and blood transfusion [odds ratio (OR)= 0.53, 95%CI: 0.29 to 0.95, P = 0.03], faster recovery of intestinal function (WMD =-0.88 d, 95%CI:-1.58 to -0.19, P = 0.01) and diet (WMD =-1.54 d, 95%CI:-2.30 to -0.78, P < 0.0001), shorter length of postoperative hospital stay (WMD =-4.06 d, 95%CI:-5.95 to -2.18, P < 0.0001), and lower rates of surgical complications (OR = 0.60, 95%CI: 0.37 to 0.99, P = 0.04). However, the operation time, rates and severity of overall complications, and rates of general complications showed no significant differences between the MIS and OS groups. M

关键词： Minimally invasive surgical procedures Laparotomy Colorectal neoplasms Synchronous liver metastases Meta-analysis

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Microscopic characterization and formation mechanisms of deep-water sandy-debris-flow and turbidity-current sandstones in a lacustrine basin： a case study in the Yanchang Formation of the Ordos Basin, China

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Petroleum Science 2018年第1期15卷 28-40页

作者： Jian-Bo liao ai-hua Xi Zhi-Yong Li hua-Qing Liu Xiang-Bo Li Rong Wanyan School of Geoscience and Technology Southwest Petroleum University Chengdu 610500 Sichuan China Petro China Research Institute of Petroleum Exploration &Development-Northwest Lanzhou 730020 Gansu China Key Laboratory of Reservoir Description CNPCLanzhou 730020 Gansu China

Deep-water deposition is a current issue in sedimentological research. Sandy-debris-flow sandstones and turbidity-current sandstones are the main types of sandstone that are the focus of considerable disputes in this research. Previous studies mainly focused on description of the macroscopic sedimentary structure and theoretical derivation of the formation mechanisms. The microscopic petrological characteristics, reservoir properties, and formation mechanisms of deep-water sandy-debris-flow and turbidity-current sandstones have been studied in the Yanchang Formation of the Ordos Basin,China, by means of field outcrop surveys, thin-section identification, geochemical element analysis, and porosity and permeability measurements under overburden pressure. The content of detrital grains in the sandy-debris-flow sandstones is high, whereas the contents of mica sheets and matrix are low. The fine-grained matrix is distributed unevenly within the pores. A considerable number of residual intergranular pores are preserved in the middle of single sand bodies, resulting in relatively better reservoir properties. The total number of detrital grains in the turbidite sandstone is low, while it contains abundant mica sheets and matrix. The mica sheets and fine-grained matrix are distributed evenly within the pores, resulting in serious damage to pores and poor reservoir properties. The sandy-debris-flow sandstones in the center of the lake basin form a high-quality reservoir; thus, this area is suitable for oil and gas exploration.

关键词： Sandy debris flow Turbidite Ordos Basin Petrology Reservoir properties

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广西扶绥县人群DDX39基因单核苷酸多态性与肝癌家系遗传易感性的关系

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中国癌症防治杂志 2016年第6期8卷 344-348页

作者：任爱华廖燕韦柳君赵瑞强魏斐斐谢裕安广西医科大学附属肿瘤医院广西壮族自治区肿瘤防治研究所南宁530021 国家生物靶向诊治国际联合研究中心广西生物靶向诊治研究重点实验室广西肿瘤靶向诊治协同创新中心广西高校生物传感重点实验室广西医科大学研究生学院广西医科大学第三附属医院南宁市第二人民医院广西医科大学生物化学与分子生物学教研室

目的探讨广西扶绥县原发性肝细胞癌（hepatocellular carcinoma,HCC,以下简称“肝癌”）高发家系人群DDX39基因rs12461754位点单核苷酸多态性与肝癌遗传易感性的相关性。方法选取广西扶绥县20个肝癌高发家系共77例和10个正常对照家系... 详细信息

目的探讨广西扶绥县原发性肝细胞癌（hepatocellular carcinoma,HCC,以下简称“肝癌”）高发家系人群DDX39基因rs12461754位点单核苷酸多态性与肝癌遗传易感性的相关性。方法选取广西扶绥县20个肝癌高发家系共77例和10个正常对照家系共35名为研究对象,采用基质辅助激光解析电离飞行时间质谱技术（matrix assisted laser desorption ionization-time of fight-mass, MALDI-TOF-MS）检测DDX39基因rs12461754位点在两组中的基因型和等位基因频率。结果正常对照家系组人群DDX39 基因rs12461754 位点携带A等位基因型的个体发生肝癌的风险是G等位基因型个体的0.407倍（95%CI：0.125～1.326,P=0.136）;在肝癌高发家系非肝癌组人群中,携带A等位基因型的个体发生肝癌的风险是G等位基因型个体的0.981倍（95%CI：0.298～3.326,P=0.976）; DDX39基因rs12461754位点GG、AG、AA基因型在肝癌高发家系肝癌组、肝癌高发家系非肝癌组及正常对照家系组三组间相互比较,差异无统计学意义（P〉0.05）。结论 DDX39基因rs12461754位点单核苷酸多态性与广西扶绥县肝癌家系遗传易感性无明显相关性。

关键词：肝肿瘤 DDX39基因单核苷酸多态性遗传易感性

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