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Helicobacter pylori enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in human gastric epithelial cells

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World Journal of Gastroenterology 2004年第16期10卷 2334-2339页

作者： Yi-YingWu Hwei-FangTsai We-ChengLin ai-hsiangchou Hui-TingChen Jyh-ChinYang Ping-IHsu Ping-NingHsu GraduateInstituteofImmunology CollegeofMedicineNationalTaiwanUniversityTaiwanChina DepartmentofInternalMedicine NationalTaiwanUniversityHospitalTaipeiTaiwanChina

aiM: To investigate the relations between tumor necrosis factor-related apoptosis-inducing ligand (TRaiL) and Helicobacter pylori (H pylon) infection in apoptosis of gastric epithelial cells and to assess the expression of TRaiL on the surface of infiltrating T-cells in Hpylori-infected gastric METHODS: Human gastric epithelial cell lines and primary gastric epithelial cells were co-cultured with H pylori in vitro, then recombinant TRaiL proteins were added to the culture. Apoptosis of gastric epithelial cells was determined by a specific ELISA for cell death. Infiltrating lymphocytes were isolated from Hpyiori-infected gastric mucosa, and expression of TRaiL in T cells was analyzed by flow ***: The apoptosis of gastric epithelial cell lines and primary human gastric epithelial cells was mildly increased by interaction with either TRaiL or H pyiorialone. Interestingly,the apoptotic indices were markedly elevated when gastric epithelial cells were incubated with both TRaiL and H pylori(Control vs TRaiL and Hpylori. 0.51±0.06 vs 2.29±0.27,P = 0.018). A soluble TRaiL receptor (DR4-Fc) could specifically block the TRaiL-mediated apoptosis. Further studies demonstrated that infiltrating T-cells in gastric mucosa expressed TRaiL on their surfaces, and the induction of TRaiL sensitivity by H pyloriwas dependent upon direct cell contact of viable bacteria, but not CagA and VacA of H ***: H pylori can sensitize human gastric epithelial cells and enhance susceptibility to TRaiL-mediated apoptosis. Modulation of host cell sensitivity to apoptosis by bacterial interaction adds a new dimension to the immunopathogenesis of H pylori infection.

关键词：哈比特属幽门菌肿瘤坏疽细胞凋亡诱导作用因子配合体间接凋亡胃上皮细胞

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