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Metabolomic Analysis of the Anthocyanins Associated with Different Colors of Cymbidium goeringii in Guizhou, China

Phyton-International Journal of Experimental Botany 2024年第7期93卷 1455-1466页

作者： Dongbo Wu Shuang Qu Lianwen Shen Shengqun Chen Xia Jiang ai rao Yuxue Zhao Yueyun Wang College of Pharmacy Guizhou University of Traditional Chines MedicineGuiyang550025China Research Institute of Juglans Regia Guizhou Academy of ForestryGuiyang550005China Guizhou Liping Rocky Desertification Ecosystem National Observation and Research Station Qiandongnan Prefecture556200China

Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of *** that contributes to its high economic *** clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties,liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven *** varieties,including‘Jin Qian Yuan’(JQY),‘Jin Xiu Qian Yuan’(JXQY),‘Miao Jiang Su Die’(MJSD),‘Qian Ming Su’(QMS),‘Shi Chan’(SC),and‘Yang Ming Su’(YMS),as well as the *** detected 64 anthocyanins,including cyanidins,delphinidins,malvidins,pelargonidins,peonidins,petunidins,procyanidins,and *** identified six shared differentially accumulated metabolites(DAMs),including cyanidin-3-O-rutinoside,delphinidin-3-Osophoroside,pelargonidin-3-O-rutinoside,peonidin-3-O-(6-O-malonyl-beta-D-glucoside),peonidin-3-Osophoroside,and *** DAMs were enriched in the anthocyanin biosynthesis *** Encyclopedia of Genes and Genomes pathway analysis revealed that the differentially expressed metabolites were significantly enriched in the anthocyanin biosynthesis *** of the content of differentially expressed metabolites indicated that peonidin-3-O-(6-O-malonyl-beta-D-glucoside)was the key metabolite underlying color differences among *** *** B2,pelargonidin-3-O-galactoside,and naringenin might also affect the color formation of JQY and QMS,SC,and MJSD,*** results of this study shed light on the metabolic mechanism underlying flower color differences in *** at the molecular *** findings will aid future studies of the mechanism of flower color regulation in *** and have implications for the breeding of new varieties.

关键词： Cymbidium goeringii ﬂower color anthocyanins metabolomics

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High-throughput Screening of the Enantioselectivity of Hyperthermophilic Mutant Esterases from Archaeon Aeropyrum pernix K1 for Resolution of (R,S)-2-Octanol Acetate

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Chemical Research in Chinese Universities 2007年第3期23卷 319-324页

作者： ZHANG Gui-rong GAO Ren-jun ZHANG ai-jun rao Lang CAO Shu-gui Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China

To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of （R, S）-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a screening strategy including two steps, first we selected agar plate with substrate to screen the enzymatic activity; secondly we used a pH indicator to screen the enantioselectivity. This method could rapidly detect favorable mutants with high activity and enantioselectivity. A total of 96. 2% of tedious screening work can be precluded using this screening strategy. It is an effective screening for alkyl ester and can be applied to relative screening researches. The four improved mutants were screened from the mutant esterase library. Their enantioselectivities, activities, and structures were investigated at different temperatures.

关键词： High-throughput screening Enantioselectivity Hyperthermophilic esterase Directed evolution

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地方立法公众参与的概念、主体与价值

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西北大学学报（哲学社会科学版） 2008年第1期38卷 138-142页

作者：饶世权饶艾西南交通大学人文社会科学学院四川成都610031

为探讨地方立法公众参与制度的建立与完善,运用归纳分析法、实证分析、比较、价值分析等方法界定地方立法、公众参与、地方立法公众参与和公众主体,论证了地方立法公众参与的价值。认为地方立法公众参与是指作为公众的自然人、自然人体... 详细信息

为探讨地方立法公众参与制度的建立与完善,运用归纳分析法、实证分析、比较、价值分析等方法界定地方立法、公众参与、地方立法公众参与和公众主体,论证了地方立法公众参与的价值。认为地方立法公众参与是指作为公众的自然人、自然人体现的法人、社会团体直接参与地方立法的制度,其具有地方法规正当性、适应性、权威性的价值。

关键词：地方立法公众参与正当性地方适应性权威性

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学术自由与学术责任——大学学术发展的价值协调

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江西教育科研 2006年第2期 19-21页

作者：饶爱京厦门大学教育研究院

学术自由是大学理念中一个最基本的学术价值观，它不仅是学术研究者的一种权利，而且体现为大学内部所特有的独立品格和精神。自17世纪末期近代大学形成以来，学术自由的理念不断强化，内涵不断丰富，逐步成为大学之所以为大学的基础、... 详细信息

学术自由是大学理念中一个最基本的学术价值观，它不仅是学术研究者的一种权利，而且体现为大学内部所特有的独立品格和精神。自17世纪末期近代大学形成以来，学术自由的理念不断强化，内涵不断丰富，逐步成为大学之所以为大学的基础、动力源和基本理念。

关键词：大学理念学术价值观学术自由学术责任学术研究者独立品格近代大学 17世纪动力源

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Microbiomics and metabolomics insights into the microbial regulation on the formation of flavor components in the traditional fermentation process of Chinese Hongqu aged vinegar

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Food Science and Human Wellness 2024年第5期13卷 2765-2778页

作者： Shangong Tong Wenlong Li Yuandong rao Yanqin Xiao Yingyin Yan Weiling Guo Xucong Lü Jinyuan Sun Lianzhong ai Li Ni Institute of Food Science and Technology College of Biological Science and EngineeringFuzhou UniversityFuzhou 350108China Food Nutrition and Health Research Center School of Advanced ManufacturingFuzhou UniversityJinjiang 362200China School of Food Science and Technology Jiangnan UniversityWuxi 214122China Beijing Laboratory for Food Quality and Safety Beijing Technology and Business UniversityBeijing 100048China School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghai 200093China

This study aimed to investigate microbial succession and metabolic dynamics during the traditional fermentation of Hongqu aged vinegar,and explore the core functional microbes closely related to the formation of flavor *** analysis demonstrated that Lactobacillus,Acetobacter,Bacillus,Enterobacter,Lactococcus,Leuconostoc and Weissella were the predominant bacterial genera,while Aspergillus piperis,Aspergillus oryzae,Monascus purpureus,Candida athensensis,***,Penicillium ochrosalmoneum and Simplicillium aogashimaense were the predominant fungal *** analysis revealed that Acetobacter was positively correlated with the production of tetramethylpyrazine,acetoin and acetic acid,Lactococcus showed positive correlation with the production of 2-nonanone,2-heptanone,ethyl caprylate,ethyl caprate,1-hexanol,1-octanol and 1-octen-3-ol,*** and *** were positively associated with the production of diethyl malonate,2,3-butanediyl diacetate,acetoin,benzaldehyde and ***,non-volatile metabolites were also detected through ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.A variety of amino acids and functional dipeptides were identified during the traditional brewing of Hongqu aged *** analysis revealed that Lactobacillus was significantly associated with DL-lactate,indolelactic acid,D-(+)-3-phenyllactic acid,pimelic acid,pregabalin and 3-aminobutanoic *** study is useful for understanding flavor formation mechanism and developing effective strategies for the suitable strains selection to improve the flavor quality of Hongqu aged vinegar.

关键词： Hongqu aged vinegar Traditional fermentation Microbial dynamics Flavor components Bidirectional orthogonal partial least squares

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Generating synthetic as-built additive manufacturing surface topography using progressive growing generative adversarial networks

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Friction 2024年第6期12卷 1283-1298页

作者： Junhyeon SEO Prahalada rao Bart RAEYMAEKERS Department of Mechanical Engineering Virginia TechBlacksburgVA 24061USA Grado Department of Industrial and Systems Engineering Virginia TechBlacksburgVA 24061USA

Numerically generating synthetic surface topography that closely resembles the features and characteristics of experimental surface topography measurements reduces the need to perform these intricate and costly ***,existing algorithms to numerically generated surface topography are not well-suited to create the specific characteristics and geometric features of as-built surfaces that result from laser powder bed fusion(LPBF),such as partially melted metal particles,porosity,laser scan lines,and ***,we present a method to generate synthetic as-built LPBF surface topography maps using a progressively growing generative adversarial *** qualitatively and quantitatively demonstrate good agreement between synthetic and experimental as-built LPBF surface topography maps using areal and deterministic surface topography parameters,radially averaged power spectral density,and material ratio *** ability to accurately generate synthetic as-built LPBF surface topography maps reduces the experimental burden of performing a large number of surface topography ***,it facilitates combining experimental measurements with synthetic surface topography maps to create large data-sets that facilitate,*** as-built surface topography to LPBF process parameters,or implementing digital surface twins to monitor complex end-use LPBF parts,amongst other applications.

关键词： additive manufacturing surface topography synthetic surface topography generative adversarial networks

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Enhanced Hybrid Equilibrium Strategy in Fog-Cloud Computing Networks with Optimal Task Scheduling

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Computers, Materials & Continua 2024年第5期79卷 2647-2672页

作者： Muchang rao Hang Qin School of Computer Science Yangtze UniversityJingzhou434000China

More devices in the Intelligent Internet of Things(aioT)result in an increased number of tasks that require low latency and real-time responsiveness,leading to an increased demand for computational *** computing’s low-latency performance issues in aioT scenarios have led researchers to explore fog computing as a complementary ***,the effective allocation of resources for task execution within fog environments,characterized by limitations and heterogeneity in computational resources,remains a formidable *** tackle this challenge,in this study,we integrate fog computing and cloud *** begin by establishing a fog-cloud environment framework,followed by the formulation of a mathematical model for task ***,we introduce an enhanced hybrid Equilibrium Optimizer(EHEO)tailored for aioT task *** overarching objective is to decrease both the makespan and energy consumption of the fog-cloud system while accounting for task *** proposed EHEO method undergoes a thorough evaluation against multiple benchmark algorithms,encompassing metrics likemakespan,total energy consumption,success rate,and average waiting *** experimental results unequivocally demonstrate the superior performance of EHEO across all assessed ***,in the most favorable conditions,EHEO significantly diminishes both the makespan and energy consumption by approximately 50%and 35.5%,respectively,compared to the secondbest performing approach,which affirms its efficacy in advancing the efficiency of aioT task scheduling within fog-cloud networks.

关键词： Artificial intelligence of things fog computing task scheduling equilibrium optimizer differential evaluation algorithm local search

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Enhancing lithium-sulfur battery performance with In_(2)O_(3)-In_(2)S_(3)@NSC heterostructures:Synergistic effects of double barrier and catalytic transformation

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Journal of Materials Science & Technology 2024年第2期169卷 105-114页

作者： Deqing He Chunyu Zhu Yutao Huo Zhonghao rao School of Energy and Environmental Engineering Hebei University of TechnologyTianjin 300401China Hebei Key Laboratory of Thermal Science and Energy Clean Utilization Hebei University of TechnologyTianjin 300401China School of Low-carbon Energy and Power Engineering China University of Mining and TechnologyXuzhou 221116China

The sluggish redox reaction kinetics of lithium polysulfides(LiPSs)are considered the main obstacle to the commercial application of lithium-sulfur(Li-S)*** accelerate the conversion by catalysis and inhibit the shuttling of soluble LiPSs in Li-S batteries,a solution is proposed in this *** solution involves fabrication of N,S co-doped carbon coated In_(2)O_(3)/In_(2)S_(3)heterostructure(In_(2)O_(3)-In_(2)S_(3)@NSC)as a multifunctional host material for the *** In_(2)O_(3)-In_(2)S_(3)@NSC composite can reduce the Gibbs free energy for the conversion reactions of LiPSs,which results in superior *** synergy between different components in In_(2)O_(3)-In_(2)S_(3)@NSC and the unique 3D structure facilitate ion and electron transport in Li-S *** In_(2)O_(3)-In_(2)S_(3)@NSC/Li 2 S 6 cathode exhibits excellent rate capacity,with a capacity of 599 mAh g−1 at 5.5 C,and good cycle stability,with a capacity of 436 mAh g^(−1)after 1000 cycles at 1 ***,this study proposes a promising solution to improve the energy storage properties of Li-S batteries,which could potentially facilitate the commercialization of Li-S batteries.

关键词： Li-S battery Multifunctional host material Synergy Heterostructures Energy storage properties

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Allogenic microglia replacement:A novel therapeutic strategy for neurological disorders

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Fundamental Research 2024年第2期4卷 237-245页

作者： Yanxia rao Bo Peng Department of Laboratory Animal Science MOE Frontiers Center for Brain ScienceFudan UniversityShanghai 200032China Department of Neurosurgery Huashan HospitalInstitute for Translational Brain ResearchState Key Laboratory of Medical NeurobiologyMOE Frontiers Center for Brain ScienceMOE Innovative Center for New Drug Development of Immune Inflammatory DiseasesFudan UniversityShanghai 200000China Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu 226001China

Microglia are resident immune cells in the central nervous system(CNS)that play vital roles in CNS development,homeostasis and disease *** defects in microglia lead to microglial dysfunction,which in turn leads to neurological *** correction of the specific genetic defects in microglia in these disorders can lead to therapeutic *** genetic defect correction approaches are dependent on viral vectorbased genetic defect ***,the viruses used in these approaches,including adeno-associated viruses,lentiviruses and retroviruses,do not primarily target microglia;therefore,viral vector-based genetic defect corrections are ineffective in *** replacement is a novel approach to correct microglial genetic defects via replacing microglia of genetic defects with allogenic healthy *** this paper,we systematically review the history,rationale and therapeutic perspectives of microglia replacement,which would be a novel strategy for treating CNS disorders.

关键词： Microglia Replacement Microglia replacement Microglia depletion Microglia-free niche CSF1R

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GLONASS星历电文特征及其解算方法

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中国惯性技术学报 2016年第3期24卷 355-360页

作者：饶爱水刘冰李清梅张龙中国卫星海上测控部江阴214431

针对飞行试验中由于GLONASS星历解算错误导致的定位结果异常问题,研究了GLONASS星历电文下传的基本特征,即通过第1~4串电文下传,并且一个更新周期内下传60组数据完全相同的星历。基于此,提出了基于电文串标识的GLONASS星历解算基本算法... 详细信息

针对飞行试验中由于GLONASS星历解算错误导致的定位结果异常问题,研究了GLONASS星历电文下传的基本特征,即通过第1~4串电文下传,并且一个更新周期内下传60组数据完全相同的星历。基于此,提出了基于电文串标识的GLONASS星历解算基本算法,但发现该算法在电文串丢失且发生星历更新时解算出错误星历的问题。为确保星历来源于连续的1~4串电文,提出了基于时间比较的星历解算改进算法,发现GLONASS星历更新时,不保证从第1串开始,也不保证在连续的1~4串电文中更新完毕,改进算法依然无法确保获取到正确星历。最后分析了星历电文误码时的特征,提出了基于星历合法性检测的星历解算可靠算法,该算法综合考虑卫星不健康、星历更新以及电文误码等异常情况,采用轨道特性检测法和原码比对检测法验证星历合法性,采用电文串标识法充分利用有效电文数据。试验结果表明,该算法的星历误码识别率达到100%,星历更新异常识别率达到100%,获取的GLONASS星历数据正确率到100%。

关键词：电文误码星历合法性卫星不健康星历更新轨道特性

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