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牛乳酪蛋白源生物活性肽的研究进展

中国调味品 2024年第7期49卷 214-220页

作者：宋萌佳宇光海潘龙廖爱美惠明黄继红侯银臣河南工业大学生物工程学院郑州450001 中原食品实验室河南漯河462000 河南大学农学院河南开封475001 许昌学院食品与药学院河南许昌461000 河南牧业经济学院食品与生物工程学院郑州450046

酪蛋白是牛乳中的主要成分,经研究证明其也是生物活性肽的主要来源,功能性小肽主要在胃肠道消化或乳制品发酵过程中通过蛋白酶和肽酶的作用所释放。根据不同的序列、结构和氨基酸含量,所产生的多肽具有不同的功能,如调节免疫力、抗菌、... 详细信息

酪蛋白是牛乳中的主要成分,经研究证明其也是生物活性肽的主要来源,功能性小肽主要在胃肠道消化或乳制品发酵过程中通过蛋白酶和肽酶的作用所释放。根据不同的序列、结构和氨基酸含量,所产生的多肽具有不同的功能,如调节免疫力、抗菌、抗氧化、降血压和改善睡眠等功能,被认为是功能性食品的潜在成分。随着对牛乳酪蛋白源生物活性肽的深入挖掘及其生理功能作用机制的阐明,酪蛋白衍生的多肽有望成为潜在功能食品添加剂及多肽类药物,在特定食品及多肽类药物的开发领域具有重要的应用前景。文章针对牛乳酪蛋白多肽挖掘、生物学功能及作用机制的国内外研究进展进行综述,以期为牛乳酪蛋白源生物活性肽的研究及应用提供参考。

关键词：牛乳酪蛋白生物活性肽

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千瓦级风冷燃料电池堆性能的研究分析

环境技术 2024年第5期42卷 147-151,156页

作者：刘利娜廖爱群饶国燃北京理工大学珠海学院工业自动化学院珠海519088

风冷燃料电池较传统的质子交换膜燃料电池简化了冷却及供气系统,使其便携性能得到了极大的提高,但是对于风冷电堆的研究大部分趋向于小功率,本文设计、测试、研究了2kW的风冷燃料电池堆的性能,探究了实测风量和理论风量的关系,温度的分... 详细信息

风冷燃料电池较传统的质子交换膜燃料电池简化了冷却及供气系统,使其便携性能得到了极大的提高,但是对于风冷电堆的研究大部分趋向于小功率,本文设计、测试、研究了2kW的风冷燃料电池堆的性能,探究了实测风量和理论风量的关系,温度的分布情况以及氢气进出方式对电堆性能的影响。研究结果表明:电堆的实际风量仅为最大风量的80%;电堆的温度分布不均匀,靠近双极板或者电堆中间部分的温度较高;此电堆单电池片数为68片,最大功率达到了1.92kW;单进单出相对于双进双出氢气进出方式,电堆功率相当,但是电压一致性更好。

关键词：风冷燃料电池风量温度分布气体流通方式

Spatial-temporal variability of throughfall in a subtropical deciduous forest from the hilly regions of eastern China

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Journal of Mountain Science 2019年第8期16卷 1788-1801页

作者： ZHANG Hai-xia WU Hua-wu LI Jing HE Bin LIU Jiu-fu WANG Niu DUAN Wei-li liao ai-min Key Laboratory of Watershed Geographic Sciences Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China Chuzhou Hydrology Laboratory Nanjing Hydraulic Research InstitutesNanjing 210029China Tourism School Jiujiang CollegeJiujiang 332005China State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China

Throughfall variability plays a crucial role in regulating hydrological and biogeochemical processes in forest ecosystems. However, throughfall variability and its potential influencing factors remain unclear in the subtropical deciduous forest because of its complex canopy and meteorological conditions. Here, the spatial variability and temporal stability of throughfall were investigated from October 2016 to December 2017 within a deciduous forest in the subtropical hilly regions of eastern China, and the effects of meteorological variables and distance from nearest tree trunk on throughfall variability were systematically evaluated. Throughfall variability during the leafed period was slightly higher than that during the leafless period inferred from the coefficient of variation of throughfall amounts(CVTF), with 13.2%-40.9% and 18.7%-31.9%, respectively. The multiple regression model analysis suggested that the controlling factors of throughfall variability were different in studied periods: Maximum 10-min rainfall intensity, wind speed and air temperature were the dominant influencing factors on throughfall variability during the leafed period, with the relative contribution ratio(RCR) of 25.9%, 18.7% and 8.9%, respectively. By contrast, throughfall variability was affected mainly by the mean rainfall intensity(RCR=40.8%) during the leafless period. The temporal stability plots and geostatistical analysis indicated that spatial patterns of throughfall were stable and similar among rainfall events. Our findings highlight the important role of various meteorological factors in throughfall variability and are expected to contribute to the accurate assessment of throughfall, soil water and runoff within the subtropical forests.

关键词： Throughfall variability Spatial heterogeneity Temporal stability Meteorological variables Canopy structure Subtropical forest

Isolation and bioinformatics analysis of differentially methylated genomic fragments in human gastric cancer

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World Journal of Gastroenterology 2008年第9期14卷 1333-1338页

作者： ai-Jun liao Qi Su Xun Wang Bin Zeng Wei Shi Cancer Research Institute Nanhua University The First Affiliated Hospital University of South China Hengyang 421001 Hunan Province China Department of Gastroenterology The First Affiliated Hospital University of South China Hengyang 421001 Hunan Province China

aiM:To isolate and analyze the DNA sequences which are methylated differentially between gastric cancer and normal gastric mucosa. METHODS: The differentially methylated DNA sequences between gastric cancer and normal gastric mucosa were isolated by methylation-sensitive representational difference analysis (MS-RDA). Similarities between the separated fragments and the human genomic DNA were analyzed with Basic Local Alignment Search Tool (BLAST). RESULTS: Three differentially methylated DNA sequences were obtained, two of which have been accepted by GenBank. The accession numbers are AY887106 and AY887107. AY887107 was highly similar to the 11th exon of LOC440683 (98%), 3' end of LOC440887 (99%), and promoter and exon regions of DRD5 (94%). AY887106 was consistent (98%) with a CpG island in ribosomal RNA isolated from colorectal cancer by Minoru Toyota in 1999. CONCLUSION: The methylation degree is different between gastric cancer and normal gastric mucosa. The differentially methylated DNA sequences can be isolated effectively by MS-RDA.

关键词： Gastric cancer DNA methylation Differentia sequences Methylation-sensitive representationa difference analysis

A Method for Automatically Establishing a Mathematical Model of Kinematic Analysis to a Planar Mechanism with a Multiple Joint and Prismatic Pair

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International Journal of Plant Engineering and Management 2011年第2期16卷 89-96页

作者： NIE Song-hui liao ai-hong SONG Li Department of Mechanical Engineering Xiangtan University Xiangtan 411105P. R. China

A method for automatically establishing a mathematical model of kinematic analysis to a planar mechanism with multiple joint and prismatic pair is presented. The breadth （ or depth ） first search spanning tree can be obtained based on an adjacency matrix of the mechanism. Then the kinematic chain （or mechanism）＇s basic loops can be obtained. On the basis of these basic loops, a mathematical model of kinematic analysis can be established and solved automatically. In the sense of a calculative mechanism, structural analysis of the kinematic chain relates to the kinematic analysis of a mechanism. Thus, an effective way is supplied to the given mechanism＇s kinematic analysis for automatic modeling and solving, and a method is supplied to the structural type to optimize kinematic synthesis.

关键词： muhiple joint prismatic pair planar mechanism model of kinematic analysis automatic modeling

Lactobacillus plantarum AR495 improves stress-induced irritable bowel syndrome in rats by targeting gut microbiota and Mast cell-PAR2-TRPV1 signaling pathway

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Food Science and Human Wellness 2024年第2期13卷 698-708页

作者： Hongyun Zhang Guangqiang Wang Zhiqiang Xiong Zhuan liao Yangyan Qian Xin Song Li Sui Lianzhong ai Yongjun Xia Shanghai Engineering Research Center of Food Microbiology School of Health Science and EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China Department of Gastroenterol Digestive Endoscopy CenterShanghai HospitalShanghai 200093China

Probiotics have great potential in regulating intestinal *** this study,the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome(IBS)rats were *** results showed that tryptase released after mast cell activation and degranulation plays a key role in visceral pain,and *** AR495 reduced the stimulation of colonic mast cells and the expression of protease-activated receptor 2(PAR2)and TRPV1 in dorsal root *** further showed that supplementation with *** AR495 increased the level of short-chain fatty acids(SCFAs)and enhanced the barrier function of the *** addition,the microbiota analysis of the colon indicated that *** AR495 promoted the proliferation of Bifidobacterium and inhibited the proliferation of Lachnospiraceae,which alleviated the imbalance of the intestinal microbiota caused by IBS to a certain *** total,*** AR495 might reduce visceral sensitivity through the Mast cell-PAR2-TRPV1 signaling pathway by maintaining the homeostasis of the intestinal barrier.

关键词： Lactobacillus plantarum Irritable bowel syndrome Visceral sensitivity Dorsal root ganglia Tryptase Microbiota

Friction between Strongly Compressed Polymer Brushes

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Chinese Journal of Polymer Science 2024年第9期42卷 1368-1374,I0008页

作者： Qi liao Institute of Chemistry Chinese Academy of SciencesBejing 100190China

We present the results of molecular dynamics simulations of steady shear between a pair of neutral polymer brushes,as well as a pair of charged polymer brushes in the strongly compressed *** results of the molecular dynamic simulations of neutral and polyelectrolyte brushes in implicit solvent including normal forces,shear forces,viscosities and friction coefficients as a function of separation between brushes,are presented in the *** comparison of the simulation results of neutral and charged brushes shows that the charged brushes is in the quasi-neutral regime,and the dependence of viscosity on the separation distance show the similar power law of neutral *** simulation results confirm that the implicit solvent simulations of polyelectrolyte brushes that ignore hydrodynamics interaction are in agreement with the scaling predictions qualitatively because of screening of hydrodynamic interaction and long-range electrostatic interactions on the correlation length *** of neutral and charged brushes show the lubrication properties that the friction coefficient decreases with the separation decreases at enough large ***,a maximum of friction coefficients is observed for polyelectrolyte brushes,which is in contrast to the neutral brushes with monotonical dependence.

关键词： Polymer brush Frictions coefficient Polyelectrolyte Lubrication

Quick recovery and characterization of cell-free DNA in seminal plasma of normozoospermia and azoospermia： implications for non-invasive genetic utilities

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Asian Journal of Andrology 2009年第6期11卷 703-709页

作者： Hong-Gang Li Shi-Yun Huang Hui Zhou ai-Hua liao Cheng-Liang Xiong Family Planning Research Institute and Centre for Reproductive Medicine Tongji Medical College Huazhong Universityof Science and Technology Wuhan 430030 China

We established a quick and reliable method for recovering cell-free seminal DNA （cfsDNA）, by using the binding-washing-elution procedure on the DNA purification column. Low variations （below 15%） among the triplicate values of cfsDNA quantity verified the reproducibility of our cfsDNA recovery method. Similar cfsDNA yield and size distribution between seminal plasma acquired by filtration and centrifugation confirmed the presence of cfsDNA. To investigate the general characterization of cfsDNA, the quantitation and size distribution of cfsDNA from normozoospermic and azoospermic semen were analyzed by real-time PCR and electrophoresis, respectively. CfsDNA concentration in semen with normozoospermia （n = 11） was 1.34 ± 0.65 μg ·mL^-1, whereas a higher cfsDNA concentration was observed in azoospermia （2.56 ± 1.43 μg ·mL^-1, n = 9）. The continuous distribution of DNA fragments ranging from -1 kb to 15 kb and a spectrum of multiples of 180-bp fragments were observed in each normozoospermic and azoospermic sample. Distinct characteristic DNA ladder fragmentations in some azoospermic samples implicated that cfsDNA originate partly from apoptotic cells. CfsDNAs of 36 selected azoospermic patients with known information of Y chromosome microdeletion were subjected to the same microdeletion analysis by multiplex PCR and PCR amplification of sY114 （1 450 bp）. All multiplex PCR reactions with cfsDNA amplified successfully and provided the same result as leukocyte DNA. PCR amplification of sY114 gave a 1 450-bp amplicon as expected. Our data suggested the potential use of cfsDNA in search of biomarker or diagnostic procedures.

关键词： azoospermia cell-free DNA normozoospermia seminal plasma Y chromosome microdeletion

The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa

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Asian Journal of Andrology 2006年第3期8卷 301-306,386页

作者： Hong-Gang Li ai-Hua liao Xiao-Fang Ding Hui Zhou Cheng-Liang Xiong Centre of Reproductive Medicine.Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030China

aim:To investigate the distribution of cation channel of sperm 1(CATSPER1)protein and the presence of CATSPER1 mRNA in human testis and ejaculated *** influence of anti-human CATSPER1 antibody upon human sperm motility was used to evaluate the function of human CATSPER1 and to estimate its possible use as a target for ***:Human ejaculated sperm from normozoospermic donors(n=12)and liquid nitro- gen frozen human testis were used for the study of mRNA and protein expression of CATSPER1 by reverse transcliption polymerase chain reaction(RT-PCR)and immunohistochemistry,*** from nonnozoospermic donors(n=12)were individually processed using a swim-up procedure and were then incubated with CATSPER 1 antibody at final concentrations of 20,4 and 0.8μg/*** 1,2 and 6h incubation,progressive motility and fast progressive motility were measured by means of computer-assisted semen ***:CATSPER1 transcript was detected in both human testis and each human ejaculated semen ***1 protein expressed in the membrane of spermatid and was localized in the principal piece of the sperm *** application of CATSPER1 antibody at all concentrations significantly inhibited both progressive motility and fast progressive motility after 1,2 and 6h incubation,and significant dose-dependent changes were ***:CATSPER1 is meiotically and post-meiotically expressed in human testis ***1 mRNA in human ejaculated spermatozoa could be a more feasible target for study and infertility screening than testis *** addition,our results suggest that human CATSPER1 could be a possible target for immunocontraception.(Asian J Androl 2006 May:8:301-306)

关键词： (?)ation channel of sperm 1 testis spermatozoa motility immunocontraception human