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A portable single-cell analysis system integrating hydrodynamic trapping with broadband impedance spectroscopy

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Science China(Technological Sciences) 2017年第11期60卷 1707-1715页

作者： tang WenLai tang DeZhi NI ZhongHua XIANG Nan YI hong Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments School of Mechanical EngineeringSoutheast UniversityNanjing 211189China

In this paper, we present a portable single-cell analysis system with the hydrodynamic cell trapping and the broadband electrical impedance spectroscopy （EIS）. Using the least flow resistance path principle, the hydrodynamic cell trapping in serpentine arrays can be carried out in a deterministic and automatic manner without the assistance of any external fields. The experimental results show that a cell trap rate of higher than 95% can be easily achieved in our ceil trapping microdevices. Using the maximum length sequences （MLS） technique, our home-made EIS is capable of measuring the impedance spectrum ranging from 1.953 kHz to 1 MHz in approximately 0.5 ms. Finally, on the basis of the developed single-cell analysis system, we precisely monitor the trapping process of human breast tumor cells （MCF-7 cells） according to the changes of electrical impedance. The MCF-7 cells with different trapping conditions or sizes can also be clearly distinguished through the impedance signals. Our portable single-cell analysis system may provide a promising tool to monitor single cells for long periods of time or to discriminate cell types.

关键词： single-cell analysis hydrodynamic cell trapping broadband impedance spectroscopy maximum length sequences

Nucleos(t)ide analogues causes HBV S gene mutations and carcinogenesis

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Hepatobiliary & Pancreatic Diseases International 2016年第6期15卷 579-586页

作者： Meng-Lan Wang hong tang Center of Infectious Diseases West China Hospital of Sichuan University Division of Infectious Diseases State Key Laboratory of Biotherapy and Cancer CenterWest China Hospital of Sichuan Universityand Collaborative Innovation Center for Biotherapy

BACKGROUND： The long-term use of nudeos（t）ide analogues causes drug resistance and mutations in the HBV reverse tran- scriptase （RT） region of the polymerase gene. The RT region overlaps the HBV surface gene （S gene） and therefore, the mutations in the RT region simultaneously modify S gene sequence. Certain mutations in the RT region bring about truncated S proteins because the corresponding changed S gene encodes a stop codon which results in the loss of a large portion of the C-terminal hydrophobic region of HBV surface protein. The rtA181T/sW172＊, rtM204I/sW196＊ and rtV191I/sW182＊ are the most frequently reported drug-resistant mutations with C-terminal truncation, these mutations have oncogenic potential. DATA SOURCES： PubMed and Web of Science were searched using terms：＂hepatitis B virus＂, ＂HBV drug resistance mutation＂＂HBV surface protein＂＂HBV truncation＂, ＂hepatocellular carcinoma＂, ＂rtA181T/sW172＊＂, ＂rtM204I/sW196＊＂, ＂rtV191I/sW182＊＂, and relevant articles published in English in the past decades were reviewed. RESULTS： The rtA181T/sW172＊ and rtV191I/sW182＊ mutants occurred more frequently than the rtM204I/sW196＊ mutant both in chronic hepatitis B patients and the HBV-related hepatocellular carcinoma tissues. Although these mutations occur naturally, nudeos（t）ide analogues therapy is the main driving force. These mutations may exist alone or coexist with other HBV mutations. All these three mutants impair the virion secretion and result in HBV surface protein retention and serum HBV DNA level reduction. These mutations possess potential carcinogenic properties. The three mutations are resistant to more than one nucleos（t）ide analogue and therefore, it is difficult to treat the patients with the truncated ***： Nucleos（t）ide analogues induce drug resistance and HBV S gene truncated mutations. These mutations have potential carcinogenesis.

关键词： hepatitis B virus drug resistance mutation surface protein C-terminal truncation oncogenic potential hepatocellular carcinoma

Fluoride-assisted synthesis of anatase TiO_2 nanocrystals with tunable shape and band gap via a solvothermal approach

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Chinese Chemical Letters 2016年第12期27卷 1801-1804页

作者： Zhe-Ming Liu Lan Peng ai-Wei tang Department of Chemistry School of Science Beijing Jiaotong University

A simple solvothermal approach employing oleic acid has been developed to prepare anatase TiO2 nanocrystals with different shapes, which were tuned from nanorods to nano-ellipsoids by increasing the amount of NaF from 0 to 0.5 retool, and the optical band gap decreased from 3.47 eV to 3.29 eV accordingly. However, when the fluoride was changed to NH4F, the resultant TiO2 nanocrystals possessed an anatase phase but were made up of smaller-sized nanocrystals and nanorods, and the band gap was increased to 3.53 eV. The X-ray photoelectron spectroscopy （XPS） results illustrated an increase of fluorine content with an increasing amount of NaF could account for the variation of the shape and optical band gap of TiO2 nanocrystals. Moreover, the absence of fluorine content brought about less change of shape and increase of optical band gap of the product synthesized in the presence of NH4F. This result may offer another way to alter the shape and band gap of metal oxide nanocrystals with the assistance of fluoride.

关键词： Anatase TiO2 Fluoride Synthesis Optical band gap Solvothermal approach

The potential mechanism and clinical application value of remote ischemic conditioning in stroke

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Neural Regeneration Research 2025年第6期20卷 1613-1627页

作者： Yajun Zhu Xiaoguo Li Xingwei Lei Liuyang tang Daochen Wen Bo Zeng Xiaofeng Zhang Zichao Huang Zongduo Guo The First Affiliated Hospital of Chongqing Medical University ChongqingChina

Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.

关键词： Akt apoptosis autophagy cerebral perfusion cerebral vascular stenosis clinical transformation hemorrhagic stroke ischemic stroke neuroinflammation neuroprotection Notch1 PI3K remote ischemic conditioning stroke

Highly Sensitive Spectrofluorimetric Determination of Trace Amounts of Indium(Ⅲ) with Salicylaldehyde Salicyloylhydrazone

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Chemical Research in Chinese Universities 2002年第2期18卷 183-188页

作者： HAN Fang WANG hong jian tang Bo Department of Chemistry Shandong Normal UniversityJinan 250014P.R.China

The fluorescent reaction of salicylaldehyde salicyloylhydrazone(SASH) with indium(Ⅲ) was studied in detail. Based on this chelation reaction, a sensitive spectrofluorimetric method was developed for the determination of indium in a water ethanol(volume ratio 3∶7) medium at pH 4 0. Under the condition, the In SASH complex displays an excitation and emission maxima at 396 and 461 nm, respectively. The linear range of the method is from 4 7 to 1000 ng/mL and the detection limit is 0 94 ng/mL. The molar ratio of indium to the reagent is 1∶1. The interferences of other ions were studied. The method was successfully applied to the determination of indium in the chemical reagents of lead, tin, zinc, zinc chloride and geological samples by standard addition method.

关键词： Indium determination Salicylaldehyde salicyloylhydrazone Spectrofluorimetry

EFFECTIVE WIDTH AND DISTRIBUTION COEFFICIENTS OF MOMENT IN RC CONTINUOUS SLABS

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China Ocean Engineering 1990年第3期5卷 345-358页

作者： tang, Taiping hong, Xiaolin Nanjing Hydraulic Research Inst Nanjing China

Using effective width of load and distribution coefficient of moment is a kind of simplified method in the design of continuous reinforced concrete slabs, which is widely used in engineering design. In this paper, through the analysis of load-bearing states of continuous slabs restricted by the main beams under concentrated loads, the effective width of load and distribution coefficients of moment of a continuous slab are obtained with the principle of equivalent loads. On the basis of the data of a 5-span continuous reinforced concrete slab, the formula of effective width is deduced and the distribution coefficients of moment for the mid-span and supports are given.

关键词： Beams and Girders Stresses Strain Gages Measurements

An Optimal Stability Matching Algorithm for DAG Blockchain Based on Matching Theory

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Chinese Journal of Electronics 2021年第2期30卷 367-377页

作者： XIA Xu HUANG Jianhua ZHENG hong tang Ruicong East China University of Science and Technology

IOTA is a typical blockchain designed for IoT applications. The Markov chain monte carlo algorithm(MCMC) used in IOTA may lead to a large number of unverified blocks, which increases transaction delay to a certain extent. We propose a Stable matching algorithm(SMA) based on matching theory to stimulate nodes to verify blocks, thereby reducing the number of unverified blocks and the consensus delay. The structure of our Io T blockchain uses the Directed acyc1 ic graph(DAG)to improve the transaction processing capability. The nodes in the network are abstracted as transaction issuers and transaction verifiers. A verification service scheduling system is used to assign transactions to the verifiers and achieve the optimal matching. We designed a trust evaluation mechanism which offers verifiers references and awards to check transactions. The simulation results show that SMA can significantly reduce the number of orphan blocks and improve the transaction throughput, which helps to improve the reliability of the Io T blockchain.

关键词： stable matching algorithm directed acyclic graph verification service scheduling system blockchains SMA Markov processes transaction throughput IoT applications transaction verifiers transaction processing consensus delay Markov chain Monte Carlo algorithm Monte Carlo methods DAG blockchain optimal stability matching algorithm Internet of Things directed graphs scheduling computer network security transaction processing capability transaction issuers IoT blockchain IOTA orphan blocks

Control Method for Additional Damper in Hydro-turbine Speed Governor of Hydro-dominant Power Systems

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CSEE Journal of Power and Energy Systems 2023年第2期9卷 589-598页

作者： Guanhong Wang Yong tang Ying Li Dongping ai Gang Chen Wei Wei Department of Power System China Electric Power Research InstituteBeijing 100192China Department of Power System Technology State Grid Sichuan Electric Power Research InstituteChengdu 610072China

With the rapid development of the national interconnected power grid,fast automatic excitation regulators and fast turbine governors are being widely *** the current systems,some power plants adjust their responses for turbine governor systems to a very sensitive level to meet the primary frequency regulation assessment index,which actively brings about negative *** these circumstances,the ultralow frequency oscillation(ULFO)phenomenon emerged in hydrodominant power *** order to solve the problem of ULFO,the hydro-turbine governor additional damper(GAD)based on phase compensation,which adapts to ULFO,is *** design of the control system is relatively *** a phase compensation based on the field testing and modeling of the turbine governor,GAD can provide the power system with positive damping,which contributes to the stability and security of the *** to the impact of the mechanism of the turbine governor on the dynamic stability of the power system and the research of modeling and parametric testing,the proposed method not only supports positive damping,but also eliminates the negative damping of the speed governing system,which can significantly suppress the ultralow frequency oscillation and make it feasible to configure the GAD parameters according to field testing.

关键词： Governor additional damper phase compensation power system ULFO

Catalyst Control of Interfacial Welding Mechanical Properties of Vitrimers

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Chinese Journal of Polymer Science 2022年第6期40卷 611-617页

作者： Zong-tang Lv hong-Kun Yang Dong Wang State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China

Interfacial welding of thermosetting polymers has been a challenge,but vitrimers with dynamic covalent networks open numerous opportunities for welding and adhesion of these *** this work,we performed interfacial welding between epoxy-based vitrimers and an epoxy vitrimer and a thermoplastic polyurethane(TPU).Catalyst-controlled interfacial mechanical properties for both the vitrimer/vitrimer and vitrimer/TPU are observed,that is,the more efficient the catalyst for the transesterification reactions,the larger the interfacial fracture energy is,and the better the welding strength will *** interfacial mechanical properties are also found to be independent of the original mechanical properties of the *** for a vitrimer with poor mechanical properties,both the welded vitrimer/vitrimer and vitrimer/TPU exhibit larger interfacial fracture energy than the one with better mechanical properties as long as the former uses more efficient catalyst.

关键词： Vitrimers Interfacial welding Epoxy Catalyst Thermoplastic polyurethane