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PCR Based Detection and Phylogenetic Analysis of Fowl Adenovirus Strains Isolated from 2019 Epidemic from Punjab and Sindh, Pakistan

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American Journal of Molecular Biology 2020年第3期10卷 246-258页

作者： Nadeem Sharif Muhammad Danish Mehmood Syed Zeeshan haider Naqvi Huma Anwar ul-Haq Syed Shoaib ahmed Muhammad Usman Ghani Muhammad Shoaib Mubashir Hussain Institute of Molecular Biology and Biotechnology University of Lahore Lahore Pakistan Ottoman Pharma (Immuno Division) Lahore Pakistan College of Veterinary Sciences FSH & VS Peshawar Pakistan Kohat University of Science & Technology Kohat Pakistan

Hydro-Pericardium Hepatitis (HPH) is an emerging infectious disease of commercial poultry, caused by different serotypes of Fowl Adeno Virus. The vertical transmission of the virus into the progeny may results in devastating damage, causing huge economic losses to its farmers. In present study, molecular typing is performed on basis of partially conserved hexon gene sequences, using a unique set of primers having common reverse oligo for simultaneous detection of FAdV1, FAdV-4 and FAdV-11. A total of 14 fowl adeno virus strains were isolated from 100 suspected adeno virus liver samples, collected from different districts in Pakistan, between 2018 and 2019. FASTA’s sequence alignment and phylogenetic analysis revealed that out of the 14, one isolate which belonged to group A showed 27% similarity with FAdV-1, while three isolates showed 99%, 95% & 45% similarity to FAdV-4 (Group C). Whereas, ten isolates showed more than 99% similarity to FAdV-11 (Group D). The serotypes FAdV1, FAdV-4 and FAdV-11 are prevailing in the breeder and broilers. These results hold great importance in rapid, reliable and simultaneous detection of the three FAdV serotypes. Therefore, fowl adeno virus vaccine production for commercial poultry shall be according to the prevalent field serotypes.

关键词： Hydro-Pericardium Hepatitis (HPH) Hexon Gene Polymerase Chain Reaction Phylogenetic Analysis Sanger’s Sequence

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Surgical Management for a Broken Delivery Shaft in <i>Vivo</i>during PCI Following Acute Myocardial Infarction in a Complex LAD Artery Lesion: A Case Report and Literature Review

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World Journal of Cardiovascular Surgery 2021年第11期11卷 104-113页

作者： Md. Abir Tazim Chowdhury Md. Zulfiqur haider Sohail ahmed S. M. A. Zulker Nine Md. Kamrul Hasan Arup Khan Md. Junayed Imam Bhuiyan Mohammad Zishan Uddin Niaz ahmed Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Bangladesh Department of Cardiothoracic Anesthesia Evercare Hospital Dhaka Bangladesh

Stuck up and fracture of coronary angioplasty hardware are unwonted complications of percutaneous coronary interventions (PCI) for which surgical retrieval and management is once in a while required. We present one case of a 59-year-old diabetic, a hypertensive gentleman who attended the emergency room (ER) with central chest pain for 2 hours. After evaluation of the patient by physical and noninvasive diagnostic tests, he was diagnosed as a case of acute myocardial infarction (AMI) with stable hemodynamic. For the aforementioned findings, the patient was treated first with a thrombolytic agent in ER and then shifted to Cardiology Department for monitoring and further coronary evaluation by coronary angiography (CAG). CAG revealed essentially single vessel disease (SVD) with complex left anterior descending (LAD) artery lesion, so PCI was attempted but failed with an unfortunate incidence of the broken delivery shaft and left in vivo. Immediate decision making and surgical management for retrieval of lost angioplasty device and correction coronary lesion with revascularization save the patient from grave complication. All the series of events and management approaches of this very complex coronary artery lesion are discussed in this article.

关键词： Percutaneous Coronary Intervention (PCI) Acute Myocardial Infarction (AMI) Angioplasty Hardware Broken Delivery Shaft Complex Coronary Lesion Surgical Revascularization

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Subclavian Artery Pseudoaneurysm following Stab Injury and Its Surgical Approach: A Case Report and Literature Review

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World Journal of Cardiovascular Surgery 2021年第7期11卷 61-67页

作者： Md. Abir Tazim Chowdhury Sohail ahmed Md. Zulfiqur haider S. M. A. Zulker Nine Md. Kamrul Hasan Mohammad Sanaul Hoque Sarker Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Bangladesh

Penetrating injuries to the subclavian arteries as well as post traumatic pseudoaneurysm involving Subclavian artery (SCA) are very much uncommon. We present one case of a 21 year-old male sustained a physical assault with a post stab injury left subclavian artery pseudoaneurysm. He was referred to our facility, two days after sustaining a penetrating chest trauma over left infraclavicular area of the chest. The presentation, diagnostic procedures and surgical approach for management of this very rare injury are discussed.

关键词： Penetrating Chest Injury Subclavian Artery (SCA) Injury Pseudo Aneurysm of Subclavian Artery Diagnosis Surgical Approach

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Laubry-Pezzi Syndrome in an Adult Male Patient and Its Surgical Correction: A Case Report and Literature Review

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World Journal of Cardiovascular Surgery 2022年第10期12卷 219-228页

作者： Md. Abir Tazim Chowdhury Md. Zulfiqur haider Sohail ahmed S. M. A. Zulker Nine Arup Khan Naheed Rayhan Musfaqus Shalehin Md. Sifat Uddin Nakib Shah Alam Saiful Islam Khan Niaz ahmed Munama Magdum Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Bangladesh Department of Cardiothoracic Anesthesia Evercare Hospital Dhaka Bangladesh Department of Community Medicine Shaheed Monsur Ali Medical College Dhaka Bangladesh

This Laubry-Pezzi syndrome is rarely seen in patients with ventricular septal defect (VSD) combined with aortic regurgitation. A 20-year-old male patient presented to the Cardiothoracic and vascular surgery department with a large VSD with severe aortic regurgitation (AR). However, he was first symptomatic at 12 years of age and initially treated by medical management with the suggestion of surgical correction. Nevertheless, he was delayed getting surgical management due to his financial problem. This article reported on an adult patient with Laubry-Pezzi syndrome and his surgical correction and outcome. The association of congenital defects VSD and AR needs to be identified and corrected in early life for better outcomes.

关键词： Laubry-Pezzi syndrome Adult Ventricular Septal Defect (VSD) Aortic Regurgitation (AR) Surgical Correction of Adult VSD

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Diagnostic Dilemma with a Case of Primary Bronchopulmonary Actinomycosis Causing Unilateral Destroyed Lung: A Case Report and Literature Review

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Open Journal of Thoracic Surgery 2022年第1期12卷 1-10页

作者： Md. Abir Tazim Chowdhury Sohail ahmed Md. Zulfiqur haider S. M. A. Zulker Nine Md. Kamrul Hasan Md. Estiak Ullah Sahid Musfaqus Shalehin Md. Abdullah Al-Mahammud Kabir Niaz ahmed Munama Magdum Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Bangladesh Department of Cardiothoracic Anesthesia Evercare Hospital Dhaka Bangladesh Department of Community Medicine Shaheed Monsur Ali Medical College Dhaka Bangladesh

Among chronic pulmonary infections, pulmonary actinomycosis is a rare one, caused by a gram-positive microaerophilic bacterium called Actinomyces. Aside from cervicofacial or abdominopelvic actinomycosis, pulmonary involvement is rare, and sometimes this type of infection results in a misdiagnosis of pulmonary tuberculosis or carcinoma. Clinical presentation of pulmonary actinomycosis could be acute or subacute, with lobar involvement. However, the disease is most commonly diagnosed at the chronic phase, with patients presenting mild fever, weight loss, and occasional hemoptysis. Here, we described the case of a 30-year-old male patient, presented with a history of respiratory complaints for more than three years and was diagnosed clinically and radiologically as a case of a destroyed left lung. Later on, post-operative tissue diagnosis was confirmed—pulmonary actinomycosis.

关键词： Pulmonary Actinomycosis Destroyed Lung Diagnostic Dilemma

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Unusual Presentation of a Large Multi-Septated Cystic Hygroma: A Case Report and Comprehensive Literature Review

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Surgical Science 2023年第7期14卷 486-495页

作者： Md. Abir Tazim Chowdhury Sohail ahmed Md. Zulfiqur haider Niaz ahmed A. K. M. Fazlul Haque Nasrin Aktar Arup Khan Md. Abdullah-Al-Mahammud Kabir Md. Sifat Uddin Abrar Bin Ahsan Trishna Sarker Md. Khaled Hossain Munama Magdum Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Dhaka Bangladesh Department of Cardiothoracic Anesthesia Evercare Hospital Dhaka Dhaka Bangladesh Department of Plastic and Reconstructive Surgery Evercare Hospital Dhaka Dhaka Bangladesh Department of Community Medicine Shahid Monsur Ali Medical College Dhaka Bangladesh

Background: Cystic hygromas are primarily found in the cervicofacial, thoracic, and abdominal regions, with limited occurrences in other areas. Despite existing literature on this condition, comprehensive descriptions and MRI findings of cystic hygromas in the extremities are rare. Aim: This case report aims to present a unique instance of a cystic hygroma in the left thigh. The objective is to provide detailed insights into the characteristics of this atypical presentation. Case Presentation: The case involves a 2-year-10 month-old girl with a cystic hygroma in her left thigh. The report includes a comprehensive description of the lesion’s clinical features and diagnostic evaluation, emphasizing the MRI findings to enhance understanding of this rare occurrence. Conclusion: This case report highlights the rarity of cystic hygromas outside the cervicofacial, thoracic, and abdominal regions, explicitly focusing on the occurrence in the left thigh. By presenting detailed insights into the clinical features, MRI findings, histopathological findings, and the surgical approach employed, this report contributes to the existing knowledge on this condition in atypical locations and informs future treatment strategies.

关键词： Cystic Hygroma Lymphangioma Multi-Septated Cystic Hygroma Unusual Presentation Vascular Tumors Benign Soft Tissue Tumors

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Upper Gastrointestinal Endoscopic Outcomes in Patients with Gastrointestinal Symptoms: Retrospective Analysis at a Tertiary Care Hospital

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Open Journal of Gastroenterology 2019年第8期9卷 141-157页

作者： Iftikhar haider Naqvi Abu Talib Muqadus Tabraze Omer Mustafa Siddiqui Maheen Fatima Hussaini Neha Saleem Paryani Rida Effendi Samrah lnam Siddiqui Syeda Zuhaa Hassan Maham Munawar Zaiya Waseem Ameer ahmed Khan Zafir Zohab Hussain Khan S. Zain Naeem Rizvi Sofia Fatima Farooqui Misbah Munaf Hasan Obaid Faryal Khan Dow University of Health Sciences Department of Medicine Karachi Pakistan

Background: Endoscopic procedures are frequently performed to rule out any disease process which eventuates with either a positive or negative outcome. Association of patient demographics, clinical features with endoscopic outcomes are of paramount importance for better understanding and practice of endoscopy. Objectives: This study aimed to determine the frequency of positive endoscopic findings in patients with gastrointestinal symptoms compared to those with negative findings. As a secondary objective, the association between relevant demographics, symptoms, laboratory investigations and procedural outcomes of the participants and positive endoscopic findings were also assessed. Methods: A retrospective analysis of all the patients who underwent esophagogastroduodenoscopy from January 2017 to December 2017 was conducted. A total of 1066 endoscopy records were retrieved and using a convenience sampling technique, relevant data were manually entered to the questionnaires. Records with incomplete or inconsistent data were disregarded as per the exclusion criteria, resulting in 1011 patient records ultimately utilized for the study. All data were entered and analyzed using IBM SPSS 23.0. Results: The study comprised a total of 1011 patients where 52.2% were females and 47.8% males while the mean age of patients was 42.16 ± 15.45. Positive endoscopic findings were observed in 88.1%, where the most common finding was gastritis (49.1%). Approximately 14.5% had positive investigation findings for HBsAg and 23.1% were seropositive for anti-HCV antibody. P-values statistically significant were for age (p = 0.044), hematemesis (p = 0.002), betel quid addiction (p = 0.044), anti-HCV antibody (p = 0.043), HCV-RNA (p = 0.041) and “H. pylori” antigen (p = 0.032). Conclusion: Both genders were almost equally included where most patients presented between the 3rd and 5th decades of life. A high incidence of gastritis was noted. Positive endoscopic findings were associated with be

关键词： Esophagogastroduodenoscopy Endoscopy Gastritis Esophageal Varices

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