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Impact of Pr.sence or.Absence of Tr.halose dur.ng Vitr.fication on Viability and Development of Vitr.fied/War.ed Immatur. Dr.medar. Camel Oocytes

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Open Jour.al of Animal Sciences 2022年第3期12卷 347-359页

作者： Kar.m A. Yaqout ahmed monir.magdy r. badr.abou bakr.a. el-wishy Adel r. Moawad Amr.S. el-Shalofy Depar.ment of Ther.ogenology Faculty of Veterinary Medicine Cairo University Giza Egypt Depar.ment of AI and ET Animal Reproduction Research Institute Agriculture Research Centre Giza Egypt Division of Animal Science College of Agriculture Family Sciences and Technology Fort Valley State University Fort Valley GA USA

Vitr.fication of immatur. oocytes at the ger.inal vesicle (GV) stage is impor.ant to pr.ser.e female gametes. The standar. for.ula for.vitr.fication solutions has long been a debate. Her.in, we investigated the effect of the pr.sence or.absence of tr.halose in vitr.fication solution on viability, in vitr. matur.tion (IVM) r.tes, and development of vitr.fied/war.ed immatur. dr.medar. camel oocytes. Cumulus oocyte complexes (COCs) obtained at slaughter.fr.m the ovar.es of matur. she-camels wer. r.ndomly allocated into thr.e gr.ups;namely, contr.l gr.up, oocytes wer. dir.ctly subjected to IVM without vitr.fication, vitr.fication solution 1 (VS1) gr.up, oocytes wer. vitr.fied in a solution composed of 25% ethylene glycol (EG) plus 25% dimethyl sulfoxide (DMSO) + 0.5 M tr.halose;and vitr.fication solution 2 (VS2) gr.up, oocytes wer. vitr.fied in a solution composed of 25% EG plus 25% DMSO. Vitr.fication of COCs was conducted by open pulled str.ws (OPS) method. Following vitr.fication and war.ing, mor.hologically viable oocytes wer. matur.d in vitr. for.36 h. COCs wer. then fer.ilized and cultur.d in vitr. for.7 days. The per.entage of viable oocytes was significantly higher.(P 0.05) in VS2 than VS1 gr.up (80.0% vs. 63.3%, r.spectively). Nuclear.matur.tion, cleavage (48 h post-insemination;pi), and blastocyst r.tes (7 days pi) wer. significantly higher.(P r.ups. No significant differ.nces wer. obser.ed in oocyte matur.tion and development r.tes between VS2 and contr.l gr.ups. In conclusion, vitr.fication of immatur. dr.medar. camel oocytes in tr.halose-fr.e solution (VS2) was mor. advantageous than that in tr.halose supplemented media since it did not r.duce viability and development.

关键词： Camel GV Oocyte IVM Tr.halose Vitr.fication

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