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Rice melatonin deficiency causes premature leaf senescence via DNA methylation regulation

The Crop Journal 2024年第3期12卷 721-731页

作者： Yue Lu ahmed gharib Rujia Chen Hanyao Wang Tianyun Tao Zhihao Zuo Qing Bu Yanze Su Yaoqing Li Yanmo Luo Hamdi F.El-Mowafi Zhichao Wang Qianfeng Huang Shuting Wang Yang Xu Pengcheng Li Chenwu Xu Zefeng Yang Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education Agriculture College of Yangzhou UniversityYangzhou 225009JiangsuChina Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Jiangsu Key Laboratory of Crop Genetics and PhysiologyYangzhou UniversityYangzhou 225009JiangsuChina National Institute of Laser Enhanced Sciences(NILES) Cairo UniversityGiza 12613Egypt Rice Research Department Field Crops Research InstituteAgricultural Research CenterSakhaKafr El-Sheikh 33717Egypt

In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain *** deficiency led to transcriptional reprogramming,especially of genes involved in chlorophyll and carbon metabolism,redox regulation,and transcriptional regulation,during dark-induced leaf *** of mCG and mCHG in the melatonin-deficient rice mutants was associated with the expression change of both protein-coding genes and transposable element-related *** in gene expression and DNA methylation in the melatonin-deficient mutants were compensated by exogenous application of melatonin.A decreased S-adenosyl-L-methionine level may have contributed to the DNA methylation variations in rice mutants of melatonin deficiency under dark conditions.

关键词： Melatonin Premature leaf senescence Rice DNA methylation Epigenetic regulation

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Salivary leptin concentrations in Bruneian secondary school children

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Asian Pacific Journal of Tropical Biomedicine 2016年第4期6卷 335-337页

作者： Adi Idris ahmed gharib Khamis Nur Basirah Ghazali Michael Steele David Koh Nik Ani Tuah PAPRSB Institute of Health Sciences Universiti Brunei Darussalam Faculty of Science Universiti Brunei Darussalam Saw Swee Hock School of Public Health National University of Singapore

Objective:To investigate the association between leptin levels and body mass index in Bruneian secondary school ***:The body mass index of Bruneian secondary school students(aged 11–18years) was determined before collecting their saliva using the passive drool *** leptin concentration was determined by ***:Correlation and partial correlation(controlled for age) analyses showed no significant differences between the levels of salivary leptin of normal weight and overweight ***:No significant increase in the salivary leptin concentration was observed in overweight compared with normal weight Bruneian secondary school students.

关键词： Obesity Saliva Leptin Brunei Adolescents Body mass index

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Validation of prognostic indices in Egyptian Budd-Chiari syndrome patients: A single-center study

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World Journal of Gastroenterology 2017年第4期23卷 629-637页

作者： Mohammad Sakr Sara M Abdelhakam Soheir A Elsayed Enas H Allam Amir M Farid Waleed Abdelmoaty Azza M Hassan Mohamed Shaker Mohamed El-gharib ahmed Eldorry Department of Tropical Medicine Faculty of MedicineAin Shams University Department of Community Environmental and Occupational MedicineFaculty of MedicineAin Shams University Department of Radiodiagnosis and Interventional Radiology Faculty of MedicineAin Shams University

AIM To compare predictive ability of Budd-Chiari syndrome(BCS) prognostic indices(PIs) for one-year survival and Transjugular intrahepatic portosystemic shunt(TIPS) *** This retrospective study enrolled 194 Egyptian patients with primary BCS who presented to the BuddChiari Study Group of Ain Shams University Hospital. Calculation of the available PIs was performed using Child-Pugh and model for end-stage liver disease scores, BCS-specific PIs(Clichy, New Clichy andRotterdam) for all patients, and BCS-TIPS PI only for patients who underwent TIPS. The overall one-year survival rate and the one-year shunt patency rate for TIPS were *** The overall one-year survival rate was 69.6%, and the New Clichy PI revealed the best validity for its prediction at a cut-off value of 3.75, with sensitivity and specificity of 78% and 73.3%, respectively [area under receiver operating characteristic curve(AUC) = 0.806]. The one-year survival rate post-TIPS was 89.7%, and the BCS-TIPS score demonstrated validity for its prediction at a cut-off value of 3.92(sensitivity and specificity were 71.4% and 64.5%, respectively)(AUC = 0.715). Logistic regression analysis revealed that the New Clichy PI(P = 0.030), high serum total bilirubin(P = 0.047) and low albumin(P < 0.001) were independent factors for predicting mortality within one year. The one-year shunt patency rate in TIPS was 80.2%, and none of the PIs exhibited significant validity for its prediction. CONCLUSION The New Clichy score could independently predict the one-year survival in Egyptian BCS patients.

关键词： Budd-Chiari 症候群预示的索引新 Clichy 分数一个年幸存 Transjugular intrahepatic portosystemic 分流

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Bipartite Laparoscopic Cholecystectomy: New Technique for Avoiding Bile Duct Injury in Difficult Cases

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Surgical Science 2014年第6期5卷 252-257页

作者： ahmed E. Lasheen Khaled Safwat Wesam Amr Osama gharib Mostafa Baiomy Mohamed Riad Basem Sieda General and Laparoscopic Surgery Department Zagazig University Hospital Zagazig University Zagazig Egypt

The incidence of bile duct injury in laparoscopic cholecystectomy (LC) is still two times greater compared to classic open surgery. This study offers new procedure to avoid this complication during LC. The gall bladder was divided into two parts above the Hartmann pouch and all contents were aspirated. Then, the distal part was dissected for short distance. The proximal part was dissected dome down until reaching to cystic duct which was tied or clipped and cut. J-vac drain was put in peritoneal cavity. Between September 2012 and October 2013, overall 77 patients (53 females and 24 males) with mean age of 49 years (between 23 and 67 years) underwent bipartite laparoscopic cholecystectomy. The mean operative time was 60 minutes (between 40 and 90 minutes). The dissection of both parts of gall bladder was safe and easy as close as possible from its wall. No biliary tract injuries were recorded during or after procedure and also at follow-up period (20 months). Bipartite laparoscopic cholecystectomy is safe, easy to do, and can avoid all complications especially bile duct injuries in difficult cases.

关键词： Bipartite Laparoscopic Cholecystectomy Difficult Cases

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