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Histopathological and molecular study of Neospora caninum infection in bovine aborted fetuses

Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 990-994页

作者： Amir Kamali Hesam Adin Seifi ahmad reza movassaghi Gholam reza Razmi Zahra Naseri Department of Clinical Sciences Faculty of Veterinary MedicineFerdowsi University of Mashhad Center of Excellence in Ruminant Abortion and Neonatal Mortality Ferdowsi University of Mashhad Department of Pathobiology Faculty of Veterinary MedicineFerdowsi University of Mashhad

Objective:To estimate the extent to which abortion in dairy cows was associated with of Neospom caninum(***) and to determine the risk factors of neosporosis in dairy farms from 9 provinces in ***:Polymerase chain reaction(PCR) test was used to detect Neospora infection in the brain of 395 bovine aborted fetuses from 9 provinces of *** addition,the brains of aborted fetuses were taken for histopathological *** identify the risk factors associated with neosporosis,data analysis was performed by ***:*** was detected in 179(45%) out of 395 fetal brain samples of bovine aborted fetuses using *** the PCR-positive brain samples,only 56 samples were suited for histopathological *** characteristic lesions of Neospora infection including non-suppurative encephalitis were found in 16(28%) of PCR-positive *** risk factors including season,parity of dam,history of bovine virus diarrhea and infectious bovine rhinotracheitis infection in herd,cow's milk production,herd size and fetal appearance did not show association with the *** study showed that Neospora caused abortion was significantly more in the second trimester of pregnancy than other *** addition,a significant association was observed between Neospora infection and ***:The results showed *** infection was detected in high percentage of aborted *** addition,at least one fourth of abortions caused by Neospora *** results indicate increasing number of abortions associated with the protozoa more than reported before in Iran.

关键词： Neospora caninum Dairy herds Abortion PCR Histopathology Risk factors

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Cancer cell cycle heterogeneity as a critical determinant of therapeutic resistance

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Genes & Diseases 2024年第1期11卷 189-204页

作者： Ebrahim H.Maleki ahmad reza Bahrami Maryam M.Matin Department of Biology Faculty of ScienceFerdowsi University of Mashhad*** MashhadIran Jagiellonian Centre for Experimental Therapeutics(JCET) Jagiellonian University31-007 KrakowPoland Doctoral School of Exact and Natural Sciences Jagiellonian University30-348 KrakowPoland Industrial Biotechnology Research Group Institute of BiotechnologyFerdowsi University of Mashhad*** MashhadIran Novel Diagnostics and Therapeutics Research Group Institute of BiotechnologyFerdowsi University of Mashhad***MashhadIran Stem Cell and Regenerative Medicine Research Group Iranian Academic Center for EducationCulture and Research(ACECR)Khorasan Razavi Branch917751376 MashhadIran

Intra-tumor heterogeneity is now arguably one of the most-studied topics in tumor biology,as it represents a major obstacle to effective cancer *** tumor cells are highly diverse at genetic,epigenetic,and phenotypic levels,intra-tumor heterogeneity can be assumed as an important contributing factor to the nullification of chemotherapeutic effects,and recurrence of the *** on the role of heterogeneous subpopulations of cancer cells with varying cell-cycle dynamics and behavior during cancer progression and treatment;herein,we aim to establish a comprehensive definition for adaptation of neoplastic cells against *** discuss two parallel and yet distinct subpopulations of tumor cells that play pivotal roles in reducing the effects of chemotherapy:"resistant"and"tolerant"***,this review also highlights the impact of the quiescent phase of the cell cycle as a survival mechanism for cancer *** understanding the mechanisms under-lying the quiescence,it provides an insightful perspective on cancer stem cells(CsCs)and their dual and intertwined functions based on their cell cycle state in response to ***-over,CSCs,epithelial-mesenchymal transformed cells,circulating tumor cells(CTCs),and disseminated tumor cells(DTCs),which are mostly in a quiescent state of the cell cycle are proved to have multiple biological links and can be implicated in our viewpoint of cell cycle heterogeneity in ***,increasing our knowledge of cell cycle heterogeneity is a key to identifying new therapeutic solutions,and this emerging concept may provide us with new opportunities to prevent the dreadful cancer recurrence.

关键词： Cancer stem cells(CSCs) Carcinoma Cell cycle heterogeneity Quiescence Therapy resistance Therapy tolerance Tumor dormancy

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Molecular investigation of exoU and exoY virulence genes in Pseudomonas aeruginosa collected from hospitalized patients in North of Iran:A descriptive-analytical study

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Journal of Acute Disease 2024年第2期13卷 74-80页

作者： ahmad reza Moradi Mehrdad Gholami Lotfollah Davoodi Negar Hajilou Hamid reza Goli Sana Institute of Higher Education SariIran Department of Medical Microbiology and Virology Faculty of MedicineMazandaran University of Medical SciencesSariIran Antimicrobial Resistance Research Center Faculty of MedicineMazandaran University of Medical SciencesSariIran Molecular and Cell Biology Research Center Faculty of MedicineMazandaran University of Medical SciencesSariIran

Objective:To investigate the frequency of exoU and exoY genes in patients with Pseudomonas aeruginosa ***:In this study,100 clinical isolates of Pseudomonas aeruginosa were collected from patients hospitalized in educational-therapeutic hospitals and were identified using standard microbiological ***,the antibiotic resistance pattern of the isolates was determined by the disk agar diffusion *** bacterial DNAs were extracted by the alkaline lysis ***,the presence of exoU and exoY genes was evaluated by the PCR ***:In this study,47%,72%,29%,39%,40%,and 44%of the isolates were non-susceptible to piperacillin,aztreonam,ceftazidime,imipenem,tobramycin,and ciprofloxacin,*** addition,95%and 93%of the clinical isolates carried the exoU and exoY *** and fecal isolates had both virulence genes,while only one wound isolate had neither ***,all urinary isolates contained the exoY gene and only one isolate lacked the exoU ***,88 isolates simultaneously had both exoU and exoY ***:High prevalence of exoU and exoY genes in this region indicates a significant role of typeⅢsecretion system in pathogenesis of Pseudomonas *** typeⅢsecretion system may be a suitable target to reduce the pathogenicity of this bacterium.

关键词： Pseudomonas aeruginosa exoU exoY Virulence gene TypeⅢsecretion system PCR

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The global,prevalence,and risk factors of postoperative fever after percutaneous nephrolithotomy:A systematic review and meta-analysis

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Asian Journal of Urology 2024年第2期11卷 253-260页

作者： reza Falahatkar Siavash Falahatkar Mohammad Amin Khajavi Gaskarei Masoomeh Afzalipoor Ali Mojtahedi Neda Aligolighasemabadi ahmad Deilami Samaneh Mirzaei Dahka Mohammad-Hossein Keivanlou Alireza Jafari Urology Research Center Guilan University of Medical SciencesGuilanRashtIran Department of Surgery University of SaskatchewanSaskatoonCanada Microbiology Department School of MedicineGuilan University of Medical SciencesRashtIran Department of Internal Medicine School of MedicineGuilan University of Medical SiencesRashtIran School of Nursing and Midwifery Guilan University of Medical SciencesGuilanRashtIran

Objective:This study aimed to explore the global,prevalence,and risk factors of fever after percutaneous nephrolithotomy(PCNL)by conducting a systematic review and ***:The high-sensitivity searching was conducted without time limitation until December 30,2020 in Web of Sciences,Scopus,and PubMed based on inclusion and exclusion ***:The prevalence rates of fever and sepsis among patient undergoing PCNL were estimated 9.5%(95%confidence interval[CI]:9.3%-9.7%),and 4.5%(95%CI:4.2%-4.8%),*** tube was used in 9.96%(95%CI:9.94%-9.97%)of *** mean preoperative white blood cells of patients were 6.401×109/L;18.3%and 4.55%of patients were considered as the positive urinary culture and pyuria,*** 20.4%of patients suffered from residual *** odds ratios(ORs)of fever in patients who suffering from diabetes mellitus,hydronephrosis,staghorn stones,and blood transfusion were 4.62(95%CI:2.95-7.26),1.04(95%CI:0.81-1.34),2.57(95%CI:0.93-7.11),and 2.65(95%CI:1.62-4.35),*** who underwent PCNL in prone position were more likely to develop fever(OR:1.23;95%CI:0.75-2.00)than patients in supine ***:The current study showed that patients who suffer from diabetes mellitus,hydronephrosis,staghorn stones,nephrostomy tube or double-J stent,blood transfusion,and also patients who underwent PCNL in prone position surgery are more likely to develop a postoperative fever after PCNL.

关键词： Percutaneous nephrolithotomy Fever Hydronephrosis Diabetes Meta-analysis

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Preoperative Serum Albumin Levels and Postoperative Acute Kidney Injury in Off-Pump Coronary Artery Bypass Surgery: A Single-Center Study in Bangladesh

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World Journal of Cardiovascular Surgery 2024年第8期14卷 131-144页

作者： ahmad Pear Salahuddin Md. Abir Tazim Chowdhury Munama Magdum Dewan Iftakher reza Chowdhury Nittya Nanda Pal Md. Nahedul Morshed Md. Zafar-Al-Nimari Latifa Nasrin Farooque Ahmed Department of Cardiac Surgery National Heart Foundation Hospital and Research Institute (NHFH&RI) Dhaka Bangladesh Department of Cardiothoracic and Vascular Surgery Evercare Hospital Dhaka Dhaka Bangladesh National Institute of Preventive and Social Medicine Dhaka Bangladesh Mother and Child Welfare Center Cumilla Bangladesh

Background: Serum albumin, a vital plasma protein, helps maintain intravascular colloidal osmotic pressure, cardiac output, and renal function. Low preoperative serum albumin is linked to poor outcomes, including acute kidney injury (AKI), after off-pump coronary artery bypass (OPCAB) surgery. This study aimed to assess the relationship between preoperative serum albumin levels and early postoperative renal injury. Methods: This prospective comparative cross-sectional study was conducted from August 2019 to February 2021 at the National Heart Foundation Hospital & Research Institute, Bangladesh. It included 160 adult patients with normal preoperative renal function undergoing OPCAB. Patients were divided into two groups: Group A (serum albumin ≥ 4.0 gm/dl) and Group B (serum albumin Results: Preoperative serum albumin was significantly different between groups (Group A: 4.21 ± 0.05 gm/dl, Group B: 3.69 ± 0.04 gm/dl, p = 0.028). Group B had a higher incidence of hypertension (71.25% vs. 51.25%, p st and 3rd postoperative days were higher in Group B (p th day. Postoperative AKI occurred in 18.75% of Group A and 36.25% of Group B. Multivariate regression indicated that low preoperative serum albumin is an independent risk factor for postoperative AKI (p = 0.012, OR = 1.815, CI: 0.675 - 1.162). Conclusion: Preoperative serum albumin level is a valuable predictor of postoperative renal function. Ensuring high normal serum albumin levels before surgery can help minimize the risk of postoperative AKI.

关键词： Serum Albumin Acute Kidney Injury (AKI) Off-Pump Coronary Artery Bypass Surgery Bangladesh

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The impact of Persian oak (Quercus brantii Lindl.) on soil characteristics in a declining forest

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Journal of Forestry Research 2020年第6期31卷 2359-2366页

作者： reza Soleimani ahmad Hosseini Crop and Horticultural Science Research Department Ilam Agricultural and Natural Resources Research and Education CenterAREEOIlamIran Forests and Rangelands Research Department Ilam Agricultural and Natural Resources Research and Education CenterAREEOIlamIran

Root activity has an important impact on soil development but we have little knowledge of the interaction of the root zone and soil genetic *** aim of this investigation was to study the interactions between soil chemical characteristics and root zone processes in a declining Persian oak forest(Quercus brantii Lindl.).A randomized complete block design was used to investigate the long-term eff ect of Persian oak on soil horizons,and the chemical and biological properties in two soil zones(under and outside the canopy).Results indicate that the rhizosphere zone had signifi cantly higher total organic carbon(TOC)than outside the canopy soil in the upper soil *** subsurface horizons,water-extractable organic carbon values were signifi cantly higher in rhizosphere than in non-rhizospheric *** biomass carbon(MBC)values in the rhizospheric zone decreased from the fi rst to the second *** MBC/TOC ratio indicated signifi-cant diff erences between the rhizosphere and soils outside of the canopy,with the exception of the subsurface *** the subsurface horizon of the rhizosphere,there was greater respiration of organic carbon(ΣCO 2/TOC)than in outside of canopy *** addition,root processes infl uenced pH,nitrogen fractions,and availability of phosphorus,potassium,iron,zinc and *** erences in soil characteristics between rhizospheric and non-rhizospheric zones were signifi cant in surface horizons due to higher root density than in deeper soil *** fi ndings indicate that Persian oak(Quercus brantii Lindl.)roots infl uenced the main soil chemical properties,even in calcareous soils.

关键词： Declining forest Persian oak Rhizosphere Soil horizons

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Magnetic metal organic framework for pre-concentration of ampicillin from cow milk samples

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Journal of Pharmaceutical Analysis 2020年第4期10卷 365-375页

作者： ahmad reza Bagheri Mehrorang Ghaedi Chemistry Department Yasouj UniversityYasouj75918-74831Iran

The aim of this study is a present of a simple solvothermal synthesis approach to preparation of Cu-based magnetic metal organic framework(MMOF)and subsequently its application as sorbent for ultrasound assisted magnetic solid phase extraction(UAMSPE)of ampicillin(AMP)from cow milk samples prior to high performance liquid chromatography-Ultraviolet(HPLC-UV)*** of prepared MMOF were fully investigated by different techniques which showed the exclusive properties of proposed sorbent in terms of proper functionality,desirable magnetic property and also high specific surface *** influential factors on extraction recovery including sorbent dosage,ultrasonic time,washing solvent volume and eluent solvent volume were assessed using central composite design(CCD)based response surface methodology(RSM)as an operative and powerful optimization *** is the first report for determination of AMP using *** proposed method addressed some drawbacks of other methods and sorbents for determination of *** presented method decreases the extraction time(4 min)and also enhances adsorption capacity(250 mg/g).Moreover,the magnetic property of presented sorbent(15 emu/g)accelerates the extraction process which does not need filtration,centrifuge and precipitation *** the optimized conditions,the proposed method is applicable for linear range of 1.0-5000.0 μg/L with detection limit of 0.29 μg/L,satisfactory recoveries(≥95.0%)and acceptable repeatability(RSD less than 4.0%).The present study indicates highly promising perspectives of MMOF for highly effective analysis of AMP in complicated matrices.

关键词： Magnetic metal organic framework Ultrasound assisted magnetic solid phase extraction Ampicillin Cow milk samples

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Kinetic grain growth, shape memory and corrosion behavior of two Cu-based shape memory alloys after thermomechanical treatment

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Transactions of Nonferrous Metals Society of China 2013年第10期23卷 2896-2904页

作者： ahmad Ostovari MOGHADDAM Mostafa KETABCHI reza BAHRAMI Department of Mining and Metallurgical Engineering Amirkabir University of Technology

Metallurgical and mechanical properties along with shape memory and corrosion behavior of Cu-11.8% AI-3.7% Ni-1 %Mn and Cu-11% A1 5.6% Mn shape memory alloys （SMAs） were comparatively studied. The influence of grain refinement on the properties was studied by optical microscopy （OM）, scanning electron microscopy （SEM）, differential scanning calorimetry （DSC）, potentiodynamic polarizations and bend and tensile tests. Static recrystallization and kinetic grain growth show a rapid recrystallization in the first 15 s of annealing at 800℃ followed by grain growths. The minimum grain sizes obtained after 15 s are 90 and 260 μm for Cu-A1-Ni-Mn and Cu-A1-Mn, respectively. Tensile tests show typical three-stage curves for both alloys, and it is seen that alloys exhibit high fracture stress and strain after grain refinement. Microstructural observations show zig-zag morphology of β 1martensite in the Cu-A1-Ni-Mn and coexistence of β1 and y1 in the Cu-A1-Mn, which were confirmed by differential scanning calorimetry results. The shape memory ratios （17） of the alloys before thermomechanieal treatment, and after thermomechanical annealing at 800 ℃ for different time up to 15 min followed by water quenching, were evaluated. In addition, corrosion behavior of alloys after grain refinement was analyzed by means of potentiodynamic polarization measurements. The results showed that the anodic reactions were dominated by dissolution of copper, and Cu-AI-Ni-Mn alloy exhibits a better corrosion resistance than Cu-A1-Mn alloy.

关键词： shape memory alloys (SMAs) grain refinement corrosion shape memory properties

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Isoparametric Boundary Element Method Applied to the Rectangular and Delta Hydrofoils near the Free Surface

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China Ocean Engineering 2010年第2期24卷 321-331页

作者： Hassan GHASSEMI ahmad reza KOHANSAL Faculty of Marine Technology Amirkabir University of Technology

In this paper, the hydrodynamic characteristics and flow field around rectangular and delta hydrofoils, moving with a constant speed beneath the free surface are numerically studied by means of isoparametric boundary element method （IBEM）. The quantities （source and dipole strengths） and the geometry of the dements are represented by a linear distribution. Two types of three-dimensional hydrofoils （rectangular and delta） are selected with NACA4412 and symmetric Joukowski sections. Some numerical results of pressure distribution, lift, wave-making drag coefficients and velocity field around the hydrofoils are presented. Also, the wave pattern due to moving hydrofoil is predicted at different operational conditions. Comparisons are made between computational results obtained through this method and those from the experimental measurements and other numerical results which reveal good agreement.

关键词： isoparametric boundary element method delta and rectangular hydrofoils hydrodynamic characteristics free surface

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Burial and thermal maturity modeling of the Middle Cretaceous-Early Miocene petroleum system,Iranian sector of the Persian Gulf

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Petroleum Science 2015年第3期12卷 367-390页

作者： Zahra Sadat Mashhadi ahmad reza Rabbani Mohammad reza Kamali Maryam Mirshahani ahmad Khajehzadeh Petroleum Engineering Department Amirkabir University of Technology Research Institute of Petroleum Industry (RIPI)

The Cretaceous Kazhdumi and Gurpi forma- tions, ahmadi Member of the Sarvak Formation, and Paleogene Pabdeh Formation are important source rock candidates of the Middle Cretaceous-Early Miocene pet- roleum system in the Persian Gulf. This study characterizes generation potential, type of organic matter, and thermal maturity of 262 cutting samples （marls and argillaceous limestones） from these rock units taken from 16 fields in the Iranian sector of the Persian Gulf. In addition, the burial and thermal histories of these source rocks were analyzed by one-dimensional basin modeling. Based on the total organic carbon and genetic potential values, fair hydro- carbon generation potential is suggested for the studied samples. Based on Tma~ and vitrinite reflectance values, the studied samples are thermally immature to mature for hydrocarbon generation. The generated models indicate that studied source rocks are immature in central wells. The Gurpi and Pabdeh formations are immature and the ahmadi Member and Kazhdumi Formation are early mature in the western wells. The Pabdeh Formation is within the main oil window and other source rocks are at the late oil window in the eastern wells. The hydrocarbon expulsion from the source rocks began after deposition of related caprocks which ensures entrapment and preserva- tion of migrated hydrocarbon.

关键词： Persian Gulf. Kazhdumi Formation ahmadiMember Gurpi Formation Pabdeh Formation - MiddleCretaceous-Early Miocene petroleum system

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