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Preconditioning process for dermal tissue decellularization using electroporation with sonication

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Regenerative Biomaterials 2022年第1期9卷 22-29页

作者： mi Hee Leeand Jong-Chul Park min-ah Koo HaKyeong Jeong Seung Hee Hong Gyeung mi Seon mi Hee Lee Jong-Chul Park Cellbiocontrol Laboratory Yonsei University College of Medicine50-1 Yonsei-roSeodaemun-guSeoul 03722Republic of Korea Department of medical Engineering Graduate School of Medical ScienceBrain Korea 21 ProjectYonsei University College of Medicine50-1 Yonsei-roSeodaemun-guSeoul 03722Republic of Korea

Decellularization to produce bioscaffolds composed of the extracellular matrix(ECm)uses enzymatic,chemical and physical methods to remove antigens and cellular components from *** decellularization methods depend on the characteristics of tissues,and in particular,tissues with dense,complex structure and abundant lipid content are difficult to completely *** study enables future research on the development of methods and treatments for fabricating bioscaffolds via decellularization of complex and rigid skin tissues,which are not commonly considered for decellularization to date as their structural and functional characteristics could not be preserved after severe *** this study,decellularization of human dermal tissue was done by a combination of both chemical(0.05%trypsin-edTA,2%SDS and 1%Triton X-100)and physical methods(electroporation and sonication).After decellularization,the content of DNA remaining in the tissue was quantitatively confirmed,and the structural change of the tissue and the retention and distribution of ECm components were evaluated through histological and histochemical analysis,*** of the chemical pretreatment that increase the efficiency of physical stimulation as well as decellularization,and conditions for electroporation and sonication without the use of detergents,unlike the methods performed in previous studies,were established to enable the complete decellularization of the skin *** combinatorial decellularization treatment formed micropores in the lipid bilayers of the skin tissues while removing all cell and cellular residues without affecting the ECm ***,this procedure can be widely used to fabricate bioscaffolds by decellularizing biological tissues with dense and complex structures.

关键词： decellularization electroporation sonication extracellular matrix

Biochar-compost-based controlled-release nitrogen fertilizer intended for an active microbial community

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Frontiers of Agricultural Science and Engineering 2024年第2期11卷 326-343页

作者： Robiul Islam RUBEL Lin WEI Salman ALANAZI Abdulkarim ALDEKHAIL Anne CmCIDREIRA Xufei YANG Sanjita WASTI Samarthya BHAGIA Xianhui ZHAO Department of Agricultural and Biosystem Engineering South Dakota State UniversityBrookingsSD 57007USA Tickle College of Engineering University of Tennessee KnoxvilleKnoxvilleTN 37996USA Oak Ridge National Laboratory Oak RidgeTN 37830USA

Nitrogen(N) fertilizers in agriculture suffer losses by volatilization of N to the air, surface runoff and leaching into the soil, resulting in low N use efficiency(NUE)( 50%) and raising severe environmental pollutions. Controlledrelease nitrogen fertilizers(CRNFs) can control the release of N nutrients to NUE in crop production. Different methods were used to develop new ***, different CRNF technologies are still underdeveloped due to inadequate controlling on N releasing time and/or unsustainable diffusion. The study on the influences of CRNF processing parameters on microbial conditions are lacking when the CRNFs composed of various bio-ingredients such as biochar, composts, and biowaste. The complexity of processing methods, material biodegradability, and other physical properties make current CRNFs of questionable value in agricultural production. This research aims to develop a novel biochar-compost-based controlled-release urea fertilizer(BCRUF) to preserve microbial properties carried by the compost. The BCRUF was synthesized by pelletizing the 50:50(dry, wt/wt) mixture of biochar and compost. BCRUF was loaded with urea and then spray-coated with polylactic acid(PLA). The releasing time of two types of BCRUFs, coated and uncoated with PLA, for 80% of N release in water was up to 6 h at three different temperatures(4, 23, and 40 °C), compared to conventional urea fertilizer and commercial environmentally smart N(ESN) fertilizer. The releasing time of coated BCRUF for 80% N release in soil was up to 192 h(8 days). Fourier-transform infrared spectroscopy(FTIR) analysis revealed that no new functional groups were found in the release solution, indicating no new chemical hazards generated. The differential scanning calorimetry(DSC)tests also verified that its thermal stability could be up to 160 °C. The microbe populations in the BCRUF pellets were reduced after the pelleting and drying processes in BCRUF fabrication, but a few bacteria can endure in t

关键词： Soil microbial community biochar compost controlled-release nitrogen fertilizer polylactic acid spray coating

Performance evaluation of a novel rotational damper for structural reinforcement steel frames subjected to lateral excitations

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Earthquake Engineering and Engineering Vibration 2014年第1期13卷 75-84页

作者： m.Sanati S.E.Khadem S.mirzabagheri H.Sanati m.Y.Khosravieh Faculty of mechanical Engineering Tarbiat Modares University Structural Engineering Group Faculty of Civil and Environmental EngineeringTarbiat Modares University School of mechanical Engineering College of engineeringUniversity of Tehran

In this study,a novel rotational damper called a Rotational Friction Viscoelastic Damper(RFVD) is *** viscoelastic pads are added to the Rotational Friction Damper(RFD) in addition to the friction discs used in this conventional ***,the amount of energy dissipated by the damper increases in low excitation *** fact,the input energy to the structure is simultaneously dissipated in the form of friction and heat by frictional discs and viscoelastic *** order to compare the performance of this novel damper with the earlier types,a set of experiments were carried *** to the test results,the RFVD showed a better performance in dissipating input energy to the structure when compared to the *** seismic behavior of steel frames equipped with these dampers was also numerically evaluated based on a nonlinear time history *** numerical results verifi ed the performance of the dampers in increasing the energy dissipation and decreasing the energy input to the structural *** order to achieve the maximum dissipated energy,the dampers need to be installed in certain places called critical points in the *** appropriate approach is presented to properly fi nd these ***,the performance of the RFVDs installed at these critical points was investigated in comparison to some other confi gurations and the validity of the suggested method in increasing the energy dissipation was confi rmed.

关键词： seismic excitations rotational friction-viscoelastic damper rotational damper friction pad viscoelastic material energy dissipation

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生活剧本

现代装饰（家居） 2016年第6期 28-39页

作者： APALONDON设计团队 ed Reeve 范嘉苑

项目地址英国伦敦项目面积123m^2设计单位APALONDON英国APALONDON工作室近期与歌剧导演Dalia Ibelhauptaite以及电影戏剧导演Dexter Fletcher共同协作。完成了这两位导演位于伦敦克勒肯维尔地区圣约翰大街的一套公寓。这套公寓所在的大... 详细信息

项目地址英国伦敦项目面积123m^2设计单位APALONDON英国APALONDON工作室近期与歌剧导演Dalia Ibelhauptaite以及电影戏剧导演Dexter Fletcher共同协作。完成了这两位导演位于伦敦克勒肯维尔地区圣约翰大街的一套公寓。这套公寓所在的大楼建于上世纪30年代,前身为一家鞋厂。

关键词：歌剧导演 Dexter 戏剧导演项目地址 Fletcher 设计单位项目面积克勒 cube captured

Investigations of Viscous Dissipation in Stagnation Point Flow Past a Stretchable Riga Wall: modern Analysis of Heat Transport

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Communications in Theoretical Physics 2019年第4期71卷 377-383页

作者： Aisha Anjum N.A.mir m.Farooq m.Javed S.ahmad Department of mathematics Riphah International University

This article scrutinizes the features of viscous dissipation in the stagnation point ?ow past through a linearly stretched Riga wall by implementing Cattaneo-Christov heat ?ux model. Viscous dissipation is carried out in Cattaneo-Christov diffusion analysis for the ?rst time in this letter. As a result of Cattaneo-Christov model, some extra terms of viscous dissipation are appeared in the energy equation. These extra terms of viscous dissipation are missing in the literature. On the utilization of suitable transformations, the equations governing the problem are reduced under the boundary layer approximation into the non-linear and dimensionless ordinary differential equations. Convergent approach is utilized to solve the dimensionless governing equations. The solution thus acquired is used to highlight the effects of emerging parameters on velocity distribution and ?uid's temperature through the graphs. Features of the drag force(or skin friction co-e?cient) are graphically interpreted. It is noticed that the presence of modi?ed Hartman number helps to reduce the ?uid's temperature but enhances the velocity pro?le. Further an enlargement in the value of thermal time relaxation parameter helps to decrease the temperature distribution.

关键词： stagnation point Cattaneo-Christov theory Riga plate linear stretching viscous dissipation

RIGOROUS ESTImATES ON BALANCE LAWS IN BOUNDed DOmAINS

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Acta mathematica Scientia 2015年第4期35卷 906-944页

作者： Rinaldo m.COLOmBO Elena ROSSI Unità INdA m Università di Brescia Dipartimento di matematica ed Applicazioni Università di Milano-Bicocca

The initial-boundary value problem for a general balance law in a bounded domain is proved to be well posed. Indeed, we show the existence of an entropy solution, its uniqueness and its Lipschitz continuity as a function of time, of the initial datum and of the boundary datum. The proof follows the general lines in [4], striving to provide a rigorous treatment and detailed references.

关键词： balance laws initial-boundary value problem for balance laws

A Secure IoT-Cloud Based Healthcare System for Disease Classification Using Neural Network

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Computer Systems Science & Engineering 2022年第4期41卷 95-108页

作者： m.Vedaraj P.Ezhumalai Department of Computer Science and Engineering R.M.D.Engineering CollegeKavaraipettai601206TamilnaduIndia

The integration of the Internet of Things(IoT)and cloud computing is the most popular growing technology in the IT *** integrated cloud com-puting technology can be used in smart cities,health care,smart homes,environ-mental monitoring,*** recent days,IoT integrated cloud can be used in the health care system for remote patient care,emergency care,disease prediction,pharmacy management,***,still,security of patient data and disease predic-tion accuracy is a major *** machine learning approaches were used for effective early disease ***,machine learning takes more time and less performance while classiﬁ*** this research work,the Attribute based Searchable Honey Encryption with Functional Neural Network(ABSHE-FNN)framework is proposed to analyze the disease and provide stronger security in IoT-cloud healthcare *** this work,the Cardiovascular Disease and Pima Indians diabetes dataset are used for heart and diabetic disease classiﬁ***-ally,means-mode normalization removes the noise and normalizes the IoT data,which helps to enhance the quality of ***ﬁed Linear Unit(RLU)was applied to adjust the feature weight to reduce the training cost and error classiﬁ-*** proposed ABSHE-FNN technique provides better security and achieves 92.79%disease classiﬁcation accuracy compared to existing techniques.

关键词： Honey encryption functional neural network rectiﬁed linear unit feature selection classiﬁcation

原发性乳腺癌:化疗前和化疗期间固有磁敏感加权成像与组织学相关性研究

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国际医学放射学杂志 2011年第1期34卷 79-80页

作者： N.J.Taylor A.makris m.L.ah-See m.J.Beresford J.J.Stirling 路红 Academic Oncology Unit Mount Vernon Cancer Centre

目的探讨原发性乳腺癌固有磁敏感加权(ISW)成像与mR动态成像和组织学表现的相关性,并评价化疗前基线横向弛豫率(R2*)、T2*弛豫变化(ΔR2*)与新辅助化疗(NAC)

关键词：乳腺癌磁敏感基线相关性乳腺肿瘤原发性

Combined corneal CXL and photorefractive keratectomy for treatment of keratoconus: a review

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International Journal of Ophthalmology(English edition) 2019年第12期12卷 1929-1938页

作者： mansour m.Al-mohaimeed Department of Ophthalmology College of Medicine Qassim University

Keratoconus and iatrogenic keratectasia are the corneal ectatic disorders occurring due to biomechanical weakening of the cornea resulting in distorted images,myopia, and irregular astigmatism. Corneal collagen cross-linking(CXL) is performed to arrest keratoconus successfully. The main aim of this review is to discuss the safety and efficacy of the adjuvant therapies, such as the combination of CXL and photorefractive keratectomy(PRK) for the treatment of corneal ectatic disorders. A comprehensive literature search was performed using Pubm ed, medLINE, and Scopus using keywords ‘collagen’‘keratoconus’,‘keratectasia’,‘collagen cross-linking’,and ‘photorefractive keratectomy’. Search results were restricted to clinical studies published in English. Corneal CXL effectively arrests the progression of keratoconus by enhancing corneal rigidity. However, functional vision is not improved by cross-linking. Combining CXL to refractive surgeries such as topography-guided PRK or transepithelial PRK is found to be a safe and effective method in providing corneal stability as well as significantly improving functional visual acuity with few minor complications. This combined technique also prevents regression of keratoconus and reduce the risk of keratectasia. CXL combined with PRK is a promising therapeutic approach in ophthalmology that can be successfully used to treat progressive keratoconus and other corneal ectatic disorders and to enhance visual acuity.

关键词： corneal collagen cross-linking photorefractive keratectomy keratoconus