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ETM study of electroporation influence on cell morphology in human malignant melanoma and human primary gingival fibroblast cells

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Asian Pacific Journal of Tropical Biomedicine 2011年第2期1卷 94-98页

作者： Nina Skolucka Malgorzata Daczewska Jolanta Saczko agnieszka chwilkowska Anna Choromanska Malgorzata Kotulska Iwona Kaminska Julita Kulbacka Department of Medical Biochemistry Medical University Department of General Zoology Zoological InstituteUniversity of Wroclaw Institute of Biomedical Engineering and Instrumentation Wroclaw University of Technology

Objective:To estimate electroporation(EP) influence on malignant and normal ***: Two cell lines including human malignant melanoma(Me-43) and normal human gingival fibroblast(HCFs) were *** parameters were the following:230,1000,1 730,2 300 V/cm;30 μ s by 3 impulses for every *** viability of cells after EP was estimated by MTT assay. The ullrastructural analysis was observed by transmission electron microscope(Zeiss EM 900). Results:In the current study we observed the intracellular effect following EP on Me-43 and HGF *** the conditions applied,we did not observe any significant damage of mitochondrial activity in both cell lines treated by ***,we showed that EP in some conditions can stimulate cells to *** changes induced by EP were only visible in electron *** fibroblast cells we observed significant changes in lower parameters of EP(230 and 1 000 V/cm).After applying higher electric field intensities(2 300 V/cm) we detected many vacuoles,myelin-like bodies and swallowed endoplasmic *** melanoma cells such strong pathological modifications after EP were not observed,in comparison with control cells. The ultrastructure of both treated cell lines was changed according to the applied parameters of ***:We can claim that EP conditions are cell line *** terms of the intracellular morphology,human fibroblasts are more sensitive to electric field as compared with melanoma *** conditions should be determined for each cell *** our study,we can conclude that EP is not an invasive method for human normal and malignant cells. This technique can be safely applied in chemotherapy for delivering drugs into tumor cells.

关键词： Electroporation Electron microscopy Human melanoma cells Primary fibroblasts Cell morphology Transmission electron microscope Malignant cell Normal cell Intracellular effect Electric field Proliferation Ultrastructural analysis

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