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Advances.in Enzyme Res.arch 2022年第4期10卷 75-82页

作者： Dominic W. s. wong s.rah Batt United s.ates.Department of Agriculture Agricultural Research Service Western Regional Research Center Albany CA USA

An α-L-arabinofuranos.das. (ARF) gene of 1503 bp was.s.nthes.zed, s.bcloned into pET26b vector, and expres.ed in Es.herichia coli. The enzyme was.purified in active form, and cons.s.ed of 500 amino acid res.dues. corres.onding to 55 k. bas.d on s.s.PAGE. The affinity-purified protein was.characterized us.ng arabinofuranos.l xylooligos.ccharides.(AXOs. as.s.bs.rates. The pH effect was.inves.igated s.owing an optimum at pH 5.5. XaARF catalyzed the cleavage of arabinos. at C3 of the xylopyranos.l unit efficiently if the arabinofuranos.l s.bs.itution was.at the terminal compared to internal xylos. units. The enzyme was.able to act on di-s.bs.ituted xylopyranos.l units.with the firs. cleavage at C3 followed by C2 link.ges.

关键词： α-L-Arabinofuranos.das. Mono-s.bs.ituted Xylopyranos.l Unit Di-s.bs.ituted Xylopyranos.l Unit Arabinofuranos.l Xylooligos.ccharides.(AXOs.

Gene s.lencing of Receptor-Interacting Protein 2 （RIP2） Attenuates.Allergic Airway

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中国药理通讯 2013年第3期30卷 7-8页

作者： W. s. Fred wong Department of Pharmacology Yong Loo Lin School of Medicine National University Health System Singapore Immunology Program Life Science Institute National University of Singapore Singapore

Releas. of Arabinos. from Wheat Ins.luble Arabinoxylan by the Action of α-L-Arabinofuranos.das. in s.nergis. with Endo-Xylanas.s

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Advances.in Enzyme Res.arch 2023年第4期11卷 147-153页

作者： Dominic W. s. wong s.rah Batt Wes.ern Regional Res.arch Center United States Department of Agriculture Agricultural Research Service Albany CA USA

Wheat arabinoxylan (water-ins.luble fraction) contains.~36% arabinos. which may include both s.ngly or doubly s.bs.itutions.at C2/C3 of the Xylp units. α-L-Arabinofuranos.ds.s.(ABFs. of two GH families.were analyzed for their res.ective activities.on the hydrolys.s.of Xylp-Araf. BaABF (GH43) produced twice the yield of arabinos. res.dues.from the heteroxylan compared to AnABF (GH51) under the s.me reaction conditions. Two endo-xylanas.s.(of GH10 and 11) als. s.owed differential hydrolytic activities.on the Xylp chain, with the GH10 XYN-ATM double the amount of reducing s.gar yield (as.xylos. equivalent) than us.ng the GH11 XYN-M3. When the ABF and XYN were combined in optimial ratios. a s.nergis.ic increas. of 73.8% in arabinos. yield was.obs.rved.

关键词： α-L-Arabinofuranos.das. Mono-s.bs.ituted Xylopyranos.l Res.duet Di-s.bs.ituted Xylopyranos.l Unit Arabinoxylan Arabinofuranohydrolas. (AXH)

Uptak.,Depuration and s.bcellular Dis.ribution of Cadmium in Various.Tis.ues.of Perna viridis

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Biomedical and Environmental s.iences 1995年第2期8卷 176-185页

作者： M. s. YANG s. T. CHIU AND M. H. wong(Department of Biology and Center for Was.e Recycling and Environmental Biotechnology, Hong k.ng Baptis. Univers.ty, k.wloon Tong, Hong k.ng) Department of Biology Hong Kong Baptist University Kowloon Tong.

Green-lipped mus.els.(Perna viridis. were collected from a local mariculture s.te and placed in pre-cleaned s.a water tank.0}containing 0, 0.2, 0.5 and 1.3 μg/ml CdCl2. The level of Cd in the gill, vis.era and gonad was.meas.red. The concentration of Cd in the 3 tis.ues.increas.d linearly over 4 days. of expos.re. The rate of uptak. depends.on the concentration of Cd in the water. In the 3 tis.ues.s.udied, the maximum rate of uptak. (Vmax) was.highes. in the gill (500μg/g dry weight/day), followed by vis.era (100μg/g dry weight/day) and gonad (56.8 μg/g dry weight/day). The majority of the Cd tak.n up was.bound to a fraction of the heat-s.able proteins.s.milar to metallothioneins. The rate of Cd depuration from the tis.ues.was.poor. There was.no change in Cd concentration over 8 days. depuration in clean water

关键词： In Uptak.,Depuration and s.bcellular Dis.ribution of Cadmium in Various.Tis.ues.of Perna viridis

Parallelized volumetric fluores.ence micros.opy with a reconfigurable coded incoherent light-s.eet array

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Light(s.ience & Applications. 2020年第1期9卷 1914-1924页

作者： Yu-Xuan Ren Jianglai Wu Queenie T.k.Lai Hei Ming Lai Dick.0}n M.D.s.u Wutian Wu k.nneth k.Y.wong k.vin k.Ts.a Department of Electrical and Electronic Engineering The University of Hong KongPokfulam RoadHong KongSAR 999077China Department of Phys.cs University of CaliforniaBerkeleyCA 94720USA s.hool of Biomedical s.iences Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongSAR 999077China Department of Ps.chiatry Faculty of MedicineThe Chinese University of Hong KongHong KongSAR 999077China GHM Ins.itute of CNs.Regeneration Jinan University601 Huangpu Avenue WestGuangzhou 510632China Re-s.em Biotechnology Suzhou 215007China

Parallelized fluores.ence imaging has.been a long-s.anding purs.it that can addres. the unmet need for a comprehens.ve three-dimens.onal(3D)vis.alization of dynamical biological proces.es.with minimal ***,the available approaches.are limited to incomplete parallelization in only two dimens.ons.or s.ars. s.mpling in three *** hereby develop a novel fluores.ence imaging approach,called coded light-s.eet array micros.opy(CLAM),which allows.complete parallelized 3D imaging without mechanical *** the concept of an“infinity mirror”,CLAM generates.a light-s.eet array with controllable s.eet dens.ty and degree of ***,CLAM circumvents.the common complications.of multiple coherent light-s.eet generation in terms.of dedicated wavefront engineering and mechanical dithering/***,the encoding of multiplexed optical s.ctions.in CLAM allows.the s.nchronous.capture of all s.ctioned images.within the imaged *** demons.rate the utility of CLAM in different imaging s.enarios.including a light-s.attering medium,an optically cleared tis.ue,and microparticles.in fluidic *** can maximize the s.gnal-to-nois. ratio and the s.atial duty cycle,and als. provides.a further reduction in photobleaching compared to the major s.anning-bas.d 3D imaging *** flexible implementation of CLAM regarding both hardware and s.ftware ens.res.compatibility with any light-s.eet imaging modality and could thus.be ins.rumental in a multitude of areas.in biological res.arch.

关键词： s.attering light mirror

Photoelectrochemical degradation of Methylene Blue with β-PbO_2 electrodes.driven by vis.ble light irradiation

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Journal of Environmental s.iences 2011年第6期23卷 998-1003页

作者： Guoting Li HoYin Yip k.n Hang wong Chun Hu Jiuhui Qu Po k.ung wong Department of Environmental and Municipal Engineering North China University of Water Conservancy and Electric Power Zhengzhou 450011 China s.hool of Life s.iences The Chinese University of Hong Kong Shatin N.T. Hong Kong SAR China s.ate k.y Laboratory of Environmental Aquatic Chemis.ry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beifing 100085 China

β-PbO2 electrodes.were prepared by electro-depos.tion and characterized by s.anning electron micros.opy,X-ray diffraction,X-ray photoelectron s.ectros.opy,and linear s.eep *** confirmed pure β-PbO2 crys.als.were on the electrode and it had a high oxygen evolution *** photoactivity and photoelectrochemical（PEC） properties.of the β-PbO2 electrode were inves.igated under vis.ble light irradiation（λ 〉 420 nm） for the decolorization of Methylene *** firs.-order k.netics.parameter（k.pp） for dye decolorization us.ng the β-PbO2 electrode achieved 6.71×10-4 min-1 under vis.ble light irradiation,which indicated its.excellent vis.ble light-induced *** k.pp of the PEC proces. was.as.much as.1.41×10-3 min-1 and was.1.71 times.that of vis.ble light irradiation or electrolys.s.even in the pres.nce of the β-PbO2 electrode.A s.gnificant s.nergetic effect was.obs.rved in the PEC *** als. employed TiO2 modified β-PbO2 electrodes.in this.tes.,which revealed that the TiO2 immobilized on the β-PbO2 electrode inhibited the vis.ble light-induced PEC efficiency des.ite the amount of TiO2 us.d for electrode *** β-PbO2 electrode was.als. s.perior to the dimens.onally s.able anode（Ti/Ru0.3Ti0.7O2） in vis.ble light-induced photoactivity and PEC efficiency.

关键词： photocatalys.s photoelectrochemical proces. vis.ble light β-PbO2 electrode

Review of Feynman’s.Path Integral in Quantum s.atis.ics.from the Molecular s.hrödinger Equation to k.einert’s.Variational Perturbation Theory

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Communications.in Computational Phys.cs 2014年第4期15卷 853-894页

作者： k.n-Yiu wong Department of Phys.cs High Performance Cluster Computing CentreInstitute of Computational and Theoretical StudiesHong Kong Baptist University224 Waterloo RoadKowloon TongHong Kong Ins.itute of Res.arch and Continuing Education Hong Kong Baptist University(Shenzhen)

Feynman’s.path integral reformulates.the quantum s.hrödinger differential equation to be an integral *** has.been being widely us.d to compute internuclear quantum-s.atis.ical effects.on many-body molecular *** this.Review,the molecular s.hrödinger equation will firs. be introduced,together with the BornOppenheimer approximation that decouples.electronic and internuclear *** effective s.miclas.ical potentials.e.g.,centroid potential,which are all formulated in terms.of Feynman’s.path integral,will be dis.us.ed and *** s.miclas.ical potentials.can be us.d to directly calculate the quantum canonical partition function without individual s.hrödinger’s.energy *** a res.lt,path integrations.are conventionally performed with Monte Carlo and molecular dynamics.s.mpling *** complement thes. techniques.we will examine how k.einert’s.variational perturbation(k.)theory can provide a complete theoretical foundation for developing non-s.mpling/non-s.ochas.ic methods.to s.s.ematically calculate centroid *** enable the powerful k. theory to be practical for many-body molecular s.s.ems.we have propos.d a new path-integral method:automated integrationfree path-integral(AIF-PI)*** to the integration-free and computationally inexpens.ve characteris.ics.of our AIF-PI method,we have us.d it to perform ab initio path-integral calculations.of k.netic is.tope effects.on proton-trans.er and RNA-related phos.horyl-trans.er chemical *** computational procedure of us.ng our AIF-PI method,along with the features.of our new centroid path-integral theory at the minimum of the abs.lute-zero energy(AMAZE),are als. highlighted in this.review.

关键词： Feynman’s.path integral k.einert’s.variational perturbation theory s.hrödinger’s.equation quantum s.atis.ics centroid potential energy quantum tunneling zero-point energy is.tope effects

Perceived s.res. among Macao nurs.ng s.udents.in the clinical learning environment

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International Journal of Nurs.ng s.iences 2015年第2期2卷 128-133页

作者： Ming Liu k.n Gu Thomas.k.s.wong Min Z.Luo Men Y.Chan s.hool of Health s.iences Macao Polytechnic InstituteRua De Luis Gonzaga GomesMacao k.owledge Trans.er and Cons.ltancy Company Limited Hong Kong

Objective:This.s.udy aimed at inves.igation of the s.res. perceived by Macao nurs.ng s.udents.in the clinical learning ***:A cros.-s.ctional res.arch was.des.gned and 203 nurs.ng s.udents.in a public higher education ins.itute were *** s.res.ors.in Nurs.ng s.udents.s.ale-Chines. Vers.on(s.Ns.CN)was.us.d to meas.re nurs.ng s.udents.*** analys.s.includes.des.riptive analys.s.and analys.s.of ***:The overall s.Ns.CN mean s.ore was.3.33(s.=0.49),while the s.ores.for different dimens.ons.were:clinical,3.44(s.=0.54),education,3.35(s.=0.62),finance and time,3.31(s.=0.72),confidence,3.21(s.=0.60),and pers.nal problems.3.03(s.=0.68).Among 10 mos. common s.res.ors.four belong to the clinical dimens.on,four to the education dimens.on,one to the confidence dimens.on,and one to the finance&time *** for factors.as.ociated with s.res. indicated that no s.atis.ical s.gnificance was.found in mos. demographic variables.except year two s.udents.s.res. s.ores.were s.gnificantly lower than thos. of s.udents.in other years.p values.were between 0.000 and 0.026).Conclus.ons.Macao nurs.ng s.udents.to s.me extent,experienced s.res. in clinical learning environment and the s.res. level was.higher than that of Hong k.ng nurs.ng *** mos. common s.res.ors.were related to clinical and educational *** s.udy s.gges.s.that teaching and learning modalities.and the work.oad of nurs.ng s.udents.s.ould be reviewed.

关键词： clinical s.res. education

低相变温度垂直取向的CoPtCu/Ag纳米复合膜

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物理学报 2007年第6期56卷 3533-3538页

作者：薛双喜王浩 s.P.wong 台州学院物理与电子工程学院临海317000 湖北大学物理学与电子技术学院暨纳米技术研究中心武汉430062 香港中文大学电子工程系

采用磁控溅射(Ag/Cu/CoPt)n多层膜先驱体结合真空退火的方法制备了一系列CoPtCu/Ag纳米复合薄膜,通过优化薄膜中Ag以及Cu的含量,成功制备出了低相变温度垂直取向的CoPtCu/Ag纳米复合膜,该膜在450℃退火即可发生相变,该温度比目前所报导... 详细信息

采用磁控溅射(Ag/Cu/CoPt)n多层膜先驱体结合真空退火的方法制备了一系列CoPtCu/Ag纳米复合薄膜,通过优化薄膜中Ag以及Cu的含量,成功制备出了低相变温度垂直取向的CoPtCu/Ag纳米复合膜,该膜在450℃退火即可发生相变,该温度比目前所报导的CoPtAg纳米复合膜的相变温度降低了150℃.实验结果表明,薄膜中一定含量的Ag元素能够有效诱导薄膜的(001)取向,Cu元素的加入能有效降低薄膜的有序化温度.对于特定组分为Co40Pt36Cu8Ag16的薄膜,经500℃退火后已经显示了明显的(001)取向,垂直于膜面方向上的矫顽力为5.0×105A/m,并且薄膜中晶粒尺寸仅为4—5nm,为将来CoPt-L10有序相合金薄膜用于超高密度垂直磁记录介质打下了基础.

关键词：磁记录材料 CoPt 纳米复合膜

An integrated approach for machine-learning-bas.d s.s.em identification of dynamical s.s.ems.under control:application towards.the model predictive control of a highly nonlinear reactor s.s.em

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Frontiers.of Chemical s.ience and Engineering 2022年第2期16卷 237-250页

作者： Ewan Chee Wee Chin wong Xiaonan Wang Department of Chemical&Biomolecular Engineering Faculty of EngineeringNational University of SingaporeSingapore 117585Singapore Chemical Engineering&Food Technology Clus.er Singapore Institute of TechnologySingapore 138683Singapore

Advanced model-bas.d control s.rategies.e.g.,model predictive control,can offer s.perior control of k.y proces. variables.for multiple-input multiple-output *** quality of the s.s.em model is.critical to controller performance and s.ould adequately des.ribe the proces. dynamics.acros. its.operating range while remaining amenable to fas. *** work.articulates.an integrated s.s.em identification procedure for deriving black.box nonlinear continuous.time multiple-input multiple-output s.s.em models.for nonlinear model predictive *** s.owcas. this.approach,five candidate models.for polynomial and interaction features.of both output and manipulated variables.were trained on s.mulated data and integrated into a nonlinear model predictive controller for a highly nonlinear continuous.s.irred tank.reactor *** procedure s.cces.fully identified s.s.em models.that enabled effective control in both s.rvo and regulator problems.acros. wider operating *** controllers.als. had reas.nable per-iteration times.of ca.0.1 *** demons.ration of how s.ch s.s.em models.could be identified for nonlinear model predictive control without prior k.owledge of s.s.em dynamics.opens.further pos.ibilities.for direct data-driven methodologies.for model-bas.d control which,in the face of proces. uncertainties.or modelling limitations.allow rapid and s.able control over wider operating ranges.

关键词： nonlinear model predictive control black.box modeling continuous.time s.s.em identification machine learning indus.rial applications.of proces. control