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Light(Science & Applications) 2024年第1期13卷 47-54页

作者： R.Margoth Córdova-Castro Bart van Dam Alberto Lauri Stefan A.Maier Riccardo Sapienza Yannick de Wilde Ignacio Izeddin Valentina Krachmalnicoff Institut Langevin ESPCI ParisPSL UniversityCNRSParisFrance The Blackett Laboratory Department of PhysicsImperial College LondonLondonUK School of Physics and Astronomy Monash UniversityClaytonVictoriaAustralia Chair in Hybrid Nanosystems Ludwig-Maximilians Universität MünchenMuenchenGermany department of Physics University of OttawaOttawaONCanada

High refractive index dielectric nanoantennas strongly modify the decay rate via the Purcell effect through the design of radiative *** to their dielectric nature,the field is mainly confined inside the nanostructure and in the gap,which is hard to probe with scanning probe *** we use single-molecule fluorescence lifetime imaging microscopy(smFLIM)to map the decay rate enhancement in dielectric GaP nanoantenna dimers with a median localization precision of 14 *** measure,in the gap of the nanoantenna,decay rates that are almost 30 times larger than on a glass *** comparing experimental results with numerical simulations we show that this large enhancement is essentially radiative,contrary to the case of plasmonic nanoantennas,and therefore has great potential for applications such as quantum optics and biosensing.

关键词： dielectric decay radiative

Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation

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Cell Regeneration 2021年第1期10卷 329-341页

作者： Joana Esteves de Lima Frederic Relaix Univ Paris Est Creteil INSERMEnvAEFSAP-HPIMRB94010 CreteilFrance

In vertebrates, the skeletal muscles of the body and their associated stem cells originate from muscle progenitor cells,during development. The specification of the muscles of the trunk, head and limbs, relies on the activity of distinctgenetic hierarchies. The major regulators of trunk and limb muscle specification are the paired-homeobox transcriptionfactors PAX3 and PAX7. Distinct gene regulatory networks drive the formation of the different muscles of thehead. despite the redeployment of diverse upstream regulators of muscle progenitor differentiation, the commitmenttowards the myogenic fate requires the expression of the early myogenic regulatory factors MYF5, MRF4, MYOD andthe late differentiation marker MYOG. The expression of these genes is activated by muscle progenitors throughoutdevelopment, in several waves of myogenic differentiation, constituting the embryonic, fetal and postnatal phases ofmuscle growth. In order to achieve myogenic cell commitment while maintaining an undifferentiated pool of muscleprogenitors, several signaling pathways regulate the switch between proliferation and differentiation of *** identification of the gene regulatory networks operating during myogenesis is crucial for the development ofin vitro protocols to differentiate pluripotent stem cells into myoblasts required for regenerative medicine.

关键词： Myogenesis Muscle progenitor PAX3 PAX7 MRF MYF5 MYOD MYOG hiPSC ESC

Global Wheat Head detection Challenges:Winning Models and Application for Head Counting

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Plant Phenomics 2023年第3期5卷 460-473页

作者： Etienne David Franklin Ogidi Daniel Smith Scott Chapman Benoit de Solan Wei Guo Frederic Baret Ian Stavness UMR 1114 EMMAH INRAEAvignonFrance Arvalis–Institut du Végétal ParisFrance department of Computer Science University of SaskatchewanSaskatoonCanada School of Food and Agricultural Sciences University of QueenslandBrisbaneAustralia Graduate School of Agricultural and Life Sciences The University of TokyoTokyoJapan

Data competitions have become a popular approach to crowdsource new data analysis methods for general and specialized data science *** competitions have a rich history in plant phenotyping,and new outdoor field datasets have the potential to embrace solutions across research and commercial *** developed the Global Wheat Challenge as a generalization competition in 2020 and 2021 to find more robust solutions for wheat head detection using field images from different *** analyze the winning challenge solutions in terms of their robustness when applied to new *** found that the design of the competition had an influence on the selection of winning solutions and provide recommendations for future competitions to encourage the selection of more robust solutions.

关键词： Wheat specialized competition

Functional Accumulation of Antenna Proteins in Chlorophyll b-Less Mutants of Chlamydomonas reinhardtii

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Molecular Plant 2017年第1期10卷 115-130页

作者： Sandrine Bujaldon Natsumi Kodama Fabrice Rappaport Rajagopal Subramanyam Catherine de Vitry Yuichiro Takahashi FrancisAndre Wollman Institut de Biologie Physico-Chimique UMR7141 CNRS-UPMC Paris 75005 France Research Institute for Interdisciplinary Science Okayama University Okayama 700-8530 Japan JST-CREST Okayama University Okayama 700-8530 Japan department of Plant Sciences School of Life Sciences University of Hyderabad Hyderabad 500046 India These authors contributed equally to this article deceased while manuscript was in progress

The green alga Chlamydomonas reinhardtii contains several light-harvesting chlorophyll a/b complexes （LHC）- four major LHCIIs, two minor LHClIs, and nine LHCIs. We characterized three chlorophyll b-less mutants to assess the effect of chlorophyll b deficiency on the function, assembly, and stability of these chlorophyll a/b binding proteins. We identified point mutations in two mutants that inactivate the CAO gene responsible for chlorophyll a to chlorophyll b conversion. All LHClIs accumulated to wild-type levels in a CAO mutant but their light-harvesting function for photosystem II was impaired. In contrast, most LHCIs accumulated to wild-type levels in the mutant and their light-harvesting capability for photosystem I remained unaltered. Unexpectedly, LHCl accumulation and the photosystem I functional antenna size increased in the mutant compared with in the wild type when grown in dim light. When the CAO mutation was placed in a yellow-in-the-dark background （yid-BF3）, in which chlorophyll a synthesis remains limited in dim light, accumulation of the major LHClIs and of most LHCls was markedly reduced, indicating that sustained synthesis of chlorophyll a is required to preserve the proteolytic resistance of antenna pro- teins. Indeed, after crossing yid-BF3 with a mutant defective for the thylakoid FtsH protease activity, yid- BF3-ftshl restored wild-type levels of LHCI, which defines LHCI as a new substrate for the FtsH protease.

关键词： Chlamydomonas reinhardtii chlorophyll b-less mutant antenna protein CAO gene

Nonlinear gallium phosphide nanoscale photonics[Invited]

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Photonics Research 2018年第5期6卷 I0068-I0074页

作者： AUde MARTIN SYLVAIN COMBRIE ALFREDO de ROSSI GREGOIRE BEAUDOIN ISABELLE SAGNES FABRICE RAINERI Centre de Nanosciences et de Nanotechnologies CNRSUniversite Paris-SudUniversite Pans-SaclayC2N Marcoussis91460 MarcoussisFrance Thales Research and Technology France 1 avenue Augustin Fresnel91120 PalaiseauFrance Universite Paris Diderot Sorbone Paris Cite75013 ParisFrance

We introduce a nanoscale photonic platform based on gallium phosphide. Owing to the favorable material properties, peak power intensity levels of 50 GW∕cm^2 are safely reached in a suspended membrane. Consequently,the field enhancement is exploited to a far greater extent to achieve efficient and strong light–matter *** an example, parametric interactions are shown to reach a deeply nonlinear regime, revealing cascaded fourwave mixing leading to comb generation and high-order soliton dynamics.

关键词： Nonlinear gallium phosphide nanoscale photonics

Prediction of Extractable Cd,Pb and Zn in Contaminated Woody Habitat Soils Using a Change Point detection Method

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Pedosphere 2016年第3期26卷 282-298页

作者： Christophe WATERLOT Christelle PRUVOT Géraldine BIDAR Clémentine FRITSCH Annette de VAUFLEURY Renaud SCHEIFLER Francis DOUAY Groupe ISA Laboratoire Genie Civil et geo-Environnement(LGCgE)Lille 59046(France) departement Chrono-Environment UMR UFC/CNRS 6249 USC INRAUniversite Bourgogne Franche-ComteBesancon 25030(France)

Accumulation of heavy metals in soils poses a potential risk to plant production, which is related to availability of the metals in soil. The phytoavailability of metals is usually evaluated using extracting solutions such as salts, acids or chelates. The purpose of this study was to identify the most significant soil parameters that can be used to predict the concentrations of acetic and citric acidextractable cadmium(Cd), lead(Pb) and zinc(Zn) in contaminated woody habitat topsoils. Multiple linear regression models were established using two analysis strategies and three sets of variables based on a dataset of 260 soil samples. The performance of these models was evaluated using statistical parameters. Cation exchange capacity, CaCO_3, organic matter, assimilated P, free Al oxide,sand and the total metal concentrations appeared to be the main soil parameters governing the solubility of Cd, Pb and Zn in acetic and citric acid solutions. The results strongly suggest that the metal solubility in extracting solutions is extractable concentrationdependent since models were overall improved by incorporating a change point. This change point detection method was a powerful tool for predicting extractable Cd, Pb and Zn. Suitable predictions of extractable Cd, Pb and Zn concentrations were obtained, with correlation coefficient(adjusted r) ranging from 0.80 to 0.99, given the high complexity of the woody habitat soils studied. Therefore,the predictive models can constitute a decision-making support tool for managing phytoremediation of contaminated soils, making recommendations to control the potential bioavailability of metals. The relationships between acetic and/or citric acid-extractable concentrations and the concentrations of metals into the aboveground parts of plants need to be predicted, in order to make their temporal monitoring easier.

关键词： acetic acid citric acid contaminated soil extractability metals multiple linear regression model soil parameters

Habitat specialization and phylogenetic structure of tree species in a coastal Brazilian white-sand forest

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Journal of Plant Ecology 2014年第2期7卷 134-144页

作者： Alexandre A.de Oliveira Alberto Vicentini Jerome Chave Camila de T.Castanho Stuart J.Davies Adriana M.Z.Martini Renato A.F.Lima Ricardo R.Ribeiro Amaia Iribar Vinicius C.Souza Instituto de Biociências Universidade de São PauloRua do Matão321Travessa 14São PauloSP 0550890Brazil Instituto Nacional de Pesquisas da Amazônia Av.AndréAraújo2936CP 478.ManausAM 69060001Brazil Evolution et DiversitéBiologique Bâtiment 4R1 UniversitéPaul Sabatier118 route de Narbonne31062 ToulouseFrance Smithsonian Tropical Research Institute PO Box 37012WashingtonDC 20013-7012USA ESALQ Universidade de São Paulo.Avenida Pádua Dias11PiracicabaSP 13418900Brazil

Aims The coastal Brazilian rainforest on white-sand(restinga)ranks among the most fragmented forest types in the tropics,owing to both the patchy distribution of sandy soils and widespread coastal development *** we study the environmental and evolutionary determinants of a forest tree assemblage at a single restinga forest in Southeastern *** also explore the ability of competing hypotheses to explain the maintenance of species diversity in this forest type,which includes contrasting extremes of edaphic conditions associated with flooding *** The study was conducted in a white-sand forest permanent plot of 10.24 ha on the coastal plain of Southeastern *** plot was divided into 256 quadrats of 20×20 m,which were classified into two main edaphic habitats(flooded and drained).Trees with a diameter≥1 cm at breast height were *** assembled DNA sequence data for each of the 116 morphospecies recognized using two chloroplast markers(rbcL and matK).A phylogenetic tree was obtained using the maximum likelihood method,and a phylogenetic distance matrix was produced from an ultrametric *** analyzed similarity in floristic composition and structure between habitats and related them to cross-plot distances using permutation *** model torus shift simulations were performed to obtain a statistical significance level for habitat association for each *** phylogenetic structure for the two habitats and for each 20×20 m quadrat was calculated using the mean phylogenetic distance weighted by species abundance and checked for significance using the standardized effect size generated by 5000 randomizations of phylogenetic tip *** Findings Our results indicate that partitioning among edaphic habitats is important for explaining species distributions and coexistence in restinga *** distributions within the plot were found to be non-random:there was greater floristic similarity within than betwe

关键词： habitat association torus-translation test phylogenetic community structure niche partitioning edaphic condition

Chip-based squeezing at a telecom wavelength

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Photonics Research 2019年第7期7卷 10-13页

作者： F.Mondain T.Lunghi A.Zavatta E.Gouzien F.Doutre M.de Micheli S.Tanzilli V.D'Auria Universite Cote d'Azur CNRSInstitut de Physique de NiceParc Valrose06108 Nice Cedex 2France Istituto Nazionale di Ottica (INO-CNR) Largo Enrico Fermi 6 50125 FirenzeItaly LENS and department of Physics Universita di Firenze50019 Sesto FiorentinoFirenzeItaly

We demonstrate a squeezing experiment exploiting the association of integrated optics and telecom technology as key features for compact, stable, and practical continuous variable quantum optics. In our setup, squeezed light is generated by single-pass spontaneous parametric down conversion on a lithium niobate photonic circuit and detected by a homodyne detector whose interferometric part is directly integrated on the same platform. The remaining parts of the experiment are implemented using commercial plug-and-play devices based on guided-wave technologies. We measure, for a CW pump power of 40 mW, a squeezing level of -2.00±0.05 dB (anti-squeezing 2.80 ±0.05 dB), thus confirming the validity of our approach and opening the way toward miniaturized and easy-to-handle continuous variable-based quantum systems.

关键词： squeezing association single-pass

Plasma phospholipid transfer protein （PLTP） modulates adaptive immune functions through alternation of T helper cell polarization

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Cellular & Molecular Immunology 2016年第6期13卷 795-804页

作者： Catherine desrumau Stephanie Lemaire-Ewing Nicolas Ogier Akadiri Yessoufou Arlette Hammann Anabelle Sequeira-Le Grand Valerie deckert Jean-Paul Pais de Barros Naig Le Guern Julien Guy Naim A Khan Laurent Lagrost INSERM UMR866 - F21079 Dijon France Univ. Bourgogne Franche-Comte F-21000 Dijon France LipSTIC LabEx Fondation de Cooperation Scientifique Bourgogne-Franche Comte F-21000 Dijon France CHU Hopital du Bocage Dijon France plateforme de Cytometrie en Flux Universite de Bourgogne Dijon France

Objective： Plasma phospholipid transfer protein （PLTP） is a key determinant of lipoprotein metabolism, and both animal and human studies converge to indicate that PLTP promotes atherogenesis and its thromboembolic complications. Moreover, it has recently been reported that PLTP modulates inflammation and immune responses. Although earlier studies from our group demonstrated that PLTP can modify macrophage activation, the implication of PLTP in the modulation of T-cell-mediated immune responses has never been investigated and was therefore addressed in the present study. Approach and results： In the present study, we demonstrated that PLTP deficiency in mice has a profound effect on CD4＋ ThO cell polarization, with a shift towards the anti-inflammatory Th2 phenotype under both normal and pathological conditions. In a model of contact hypersensitivity, a significantly impaired response to skin sensitization with the hapten-2,4-dinitrofluorobenzene （DNFB） was observed in PLTP-deficient mice compared to wild-type （WT） mice. Interestingly, PLTP deficiency in mice exerted no effect on the counts of total white blood cells, lymphocytes, granulocytes, or monocytes in the peripheral blood. Moreover, PLTP deficiency did not modify the amounts of CD4＋ and CD8＋ T lymphocyte subsets. However, PLTP-deficiency, associated with upregulation of the Th2 phenotype, was accompanied by a significant decrease in the production of the pro-Thl cytokine interleukin 18 by accessory cells. Conclusions： For the first time, this work reports a physiological role for PLTP in the polarization of CD4＋ T cells toward the pro-inflammatory Th I phenotype.

关键词： lipid transfer protein lymphocyte polarization T cell