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Arabidopsis Protein Phosphatase 2C ABI1 Interacts with Type I ACC Synthases and Is Involved in the Regulation of Ozone-Induced Ethylene Biosynthesis

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Molecular Plant 2014年第6期7卷 960-976页

作者： Agnieszka Ludwikow agata ciesla Anna Kasprowicz-Malusk Filip Mitula Malgorzata Tajdel lukasz Gaigarnski Piotr A. Ziolkowski Piotr Kubiak Arleta Malecka Aneta Piechalak Marta Szabat Alicja Gorska Maciej Dabrowskia Izabela Ibragimow Jan Sadowski Department of Biotechnology Institute of Molecular Biology and Biotechnology Faculty of Biology Adam Mickiewicz University inPoznan Umultowska 89 61-614 Poznan Poland Institute of Plant Genetics Polish Academy of Science Strzeszynska 34 60-479 Poznan Poland Department of Molecular and Cellular Biology Institute of Molecular Biology and Biotechnology Faculty of Biology Adam MickiewiczUniversity in Poznan Umultowska 89 61-614 Poznan Poland Department of Biotechnology and Food Microbiology University of Life Sciences Wojska Polskiego 48 60-627 Poznan Poland Department of Biochemistry Institute of Molecular Bioloqy and Biotechnology Faculty of Bioloqy Adam Mickiewicz University inPoznan Umultowska 89 61-614 Poznan Poland

Ethylene plays a crucial role in various biological processes and therefore its biosynthesis is strictly regu- lated by multiple mechanisms. Posttranslational regulation, which is pivotal in controlling ethylene biosynthesis, impacts 1-aminocyclopropane 1-carboxylate synthase （ACS） protein stability via the complex interplay of specific factors. Here, we show that the Arabidopsis thaliana protein phosphatase type 2C, ABI1, a negative regulator of abscisic acid signaling, is involved in the regulation of ethylene biosynthesis under oxidative stress conditions. We found that ABI1 interacts with ACS6 and dephosphorylates its C-terminal fragment, a target of the stress-responsive mitogen-activated protein kinase, MPK6. In addition, ABI1 controls MPK6 activity directly and by this means also affects the ACS6 phosphorylation level. Consistently with this, ozone-induced ethylene production was significantly higher in an ABI1 knockout strain （abiltd） than in wild-type plants. Importantly, an increase in stress-induced ethylene production in the abiltd mutant was compen- sated by a higher ascorbate redox state and elevated antioxidant activities. Overall, the results of this study provide evi- dence that ABI1 restricts ethylene synthesis by affecting the activity of ACS6. The ABI1 contribution to stress phenotype underpins its role in the interplay between the abscisic acid （ABA） and ethylene signaling pathways.

关键词： hormonal regulation protein phosphorylation/dephosphorylation signal transduction Arabidopsis.

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Intrahepatic expression of genes related to metabotropic receptors in chronic hepatitis

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World Journal of Gastroenterology 2012年第31期18卷 4156-4161页

作者： Andrzej ciesla Maciej Kusmider agata Faron-Górecka Marta Dziedzicka-Wasylewska Monika Bocisga-Jasik Danuta Owczarek Irena Cieko-Michalska Dorota Cibor Tomasz Mach Department of Gastroenterology Hepatology and Infectious DiseasesJagiellonian University Medical College31-521 KrakówPoland Institute of Pharmacology Polish Academy of Sciences31-343 KrakówPoland

AIM:To screen for genes related to metabotropic receptors that might be involved in the development of chronic hepatitis.METHODS:Assessment of 20 genes associated with metabotropic receptors was performed in liver specimens obtained by punch biopsy from 12 patients with autoimmune and chronic hepatitis type B and C.For this purpose,a microarray with low integrity grade and with oligonucleotide DNA probes complementary to target transcripts was used.Evaluation of gene expression was performed in relation to transcript level,correlation between samples and grouping of clinical parameters used in chronic hepatitis assessment.Clinical markers of chronic hepatitis included alanine and aspartate aminotransferase,-glutamyltranspeptidase,alkaline phosphatase and cholinesterase activity,levels of iron ions,total cholesterol,triglycerides,albumin,glucose,hemoglobin,platelets,histological analysis of inflammatory and necrotic status,fibrosis according to METAVIR score,steatosis,as well as anthropometric body mass index,waist/hip index,percentage of adipose tissue and liver size in ultrasound examination.Gender,age,concomitant diseases and drugs were also taken into account.Validation of oligonucleotide microarray gene expression results was done with the use of quantitative real-time polymerase chain reaction(qRT-PCR).RESULTS:The highest(0.002 < P < 0.046) expression among genes encoding main components of metabotropic receptor pathways,such as the α subunit of G-coupled protein,phosphoinositol-dependent protein kinase or arrestin was comparable to that of angiotensinogen synthesized in the liver.Carcinogenesis suppressor genes,such as chemokine ligand 4,transcription factor early growth response protein 1 and lysophosphatidic acid receptor,were characterized by the lowest expression(0.002 < P < 0.046),while the factor potentially triggering hepatic cancer,transcription factor JUN-B,had a 20-fold higher expression.The correlation between expression of genes of protein kinases PDPK1,phosphoinositide 3-kinase and protein kina

关键词：慢性肝炎受体基因相关基因代谢型定量RT-PCR 寡核苷酸微阵列丙氨酸氨基转移酶自身免疫性疾病

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支持世界范围的野火管理

支持世界范围的野火管理

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作者： William M.ciesla 联合国粮农组织

1988年9月,吉尔伯特飓风掠过了墨西哥的尤卡坦半岛。除了造成大量的个人财产和基础设施破坏外,超过一百万公顷的热带森林也遭到大风毁坏。大量碎片造成的可燃物质增加了野火的危险。在此后,超过120,000公顷的墨西哥热带森林遭遇大火。...

1988年9月,吉尔伯特飓风掠过了墨西哥的尤卡坦半岛。除了造成大量的个人财产和基础设施破坏外,超过一百万公顷的热带森林也遭到大风毁坏。大量碎片造成的可燃物质增加了野火的危险。在此后,超过120,000公顷的墨西哥热带森林遭遇大火。三年以后,印度尼西亚遭受到少见的长期干旱。这为野火创造了有利条件,72,000公顷森林和林地被烧毁。据统计,损失达到71,000,000美元。在九月和十月,一场大火引起的浓烟,对航空和航海的影响远达曼谷和新加坡。这仅是两个例子,野火造成的影响远不止此。就全球来说,每年的野火面积平均达1200-1300万公顷。不过,野火常常是植物群落自然动力学的一个部分,它的影响可能是破

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害虫综合管理

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湖南林业科技 1984年第4期 50-52页

作者： William M.ciesla 傅生

1975年美国出版《害虫管理引论》一书(今年我国已有译本)。对什么是害虫管理有这样几句话:“Geier和Clark(1961)把害虫防治这一概念称为有害物种的保护性管理,简称害虫管理。它的一切有用技术都应作出估价,并编制统一的计划来管理害虫,... 详细信息

1975年美国出版《害虫管理引论》一书(今年我国已有译本)。对什么是害虫管理有这样几句话:“Geier和Clark(1961)把害虫防治这一概念称为有害物种的保护性管理,简称害虫管理。它的一切有用技术都应作出估价,并编制统一的计划来管理害虫,这样就能避免经济损失,并且极大地减少对环境的不良副作用(美国全国科学院,1969)。”对这篇短文,这几句话似乎是恰当的简要说明。

关键词：害虫美国黑松综合管理

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应用航空像片估计林木枯损量情况综述

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云南林业调查规划 1991年第1期20卷 46-48,F003页

作者： William M.ciesla 唐芳林

近年来,林木枯死病威胁着世界范围内的许多树种。尽管引起林木枯死的原因目前尚不清楚,但日益增长的人为引起的污染物如酸雨、臭氧、氧化硫、重金属等,很可能是导致林木易于枯死的重要原因。

关键词：航空像片林木枯损量估计

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Navigating Technological Shifts:Insights from 30+ Years of Technology Transfer

Navigating Technological Shifts:Insights from 30+ Years of T...

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第十九届中国太阳级硅及光伏发电研讨会(19thCSP...

作者： Bram Hoex Chandany Sen Muhammad Umair Khan Michael Pollard Arastoo Teymouri Xinyuan Wu Haoran Wang Jiexi Fu Catherine Chan Michelle Vaqueiro Contreras Alison ciesla Fiacre Rougieux School of Photovoltaic and Renewable Energy Engineering UNSW Sydney

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板栗果实生命表的研究

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河南农业大学学报 2001年第3期35卷 254-258页

作者：王高平 William M.ciesla 闫庆张凯河南农业大学生物工程学院河南郑州450002 Forest Health Management International

研究了河南新县不同立地条件和管理水平下的板栗果实生命表 .结果表明 :蛀果蛾是影响板栗产量的主要害虫 ,蛀果象甲在山区老板栗园危害较重。

关键词：板栗生命表蛀果蛾象甲空苞

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Performance and calibration of quark/gluon-jet taggers using 140 fb^(-1) of pp collisions at √s=13 TeV with the ATLAS detector

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Chinese Physics C 2024年第2期48卷 1-25页

作者： G. Aad B. Abbott K. Abeling N.J. Abicht S.H. Abidi A. Aboulhorma H. Abramowicz H. Abreu Y. Abulaiti B.S. Acharya C. Adam Bourdarios L. Adamczyk S.V. Addepalli M.J. Addison J. Adelman A. Adiguzel T. Adye A.A. Affolder Y. Afik M.N. Agaras J. Agarwala A. Aggarwal C. Agheorghiesei A. Ahmad F. Ahmadov W.S. Ahmed S. Ahuja X. Ai G. Aielli A. Aikot M. Ait Tamlihat B. Aitbenchikh I. Aizenberg M. Akbiyik T.P.A. Åkesson A.V. Akimov D. Akiyama N.N. Akolkar K. Al Khoury G.L. Alberghi J. Albert P. Albicocco G.L. Albouy S. Alderweireldt M. Aleksa I.N. Aleksandrov C. Alexa T. Alexopoulos F. Alfonsi M. Algren M. Alhroob B. Ali H.M.J. Ali S. Ali S.W. Alibocus M. Aliev G. Alimonti W. Alkakhi C. Allaire B.M.M. Allbrooke J.F. Allen C.A. Allendes Flores P.P. Allport A. Aloisio F. Alonso C. Alpigiani M. Alvarez Estevez A. Alvarez Fernandez M. Alves Cardoso M.G. Alviggi M. Aly Y. Amaral Coutinho A. Ambler C. Amelung M. Amerl C.G. Ames D. Amidei S.P. Amor Dos Santos K.R. Amos V. Ananiev C. Anastopoulos T. Andeen J.K. Anders S.Y. Andrean A. Andreazza S. Angelidakis A. Angerami A.V. Anisenkov A. Annovi C. Antel M.T. Anthony E. Antipov M. Antonelli F. Anulli M. Aoki T. Aoki J.A. Aparisi Pozo M.A. Aparo L. Aperio Bella C. Appelt A. Apyan N. Aranzabal S.J. Arbiol Val C. Arcangeletti A.T.H. Arce E. Arena J-F. Arguin S. Argyropoulos J.-H. Arling O. Arnaez H. Arnold G. Artoni H. Asada K. Asai S. Asai N.A. Asbah J. Assahsah K. Assamagan R. Astalos S. Atashi R.J. Atkin M. Atkinson H. Atmani P.A. Atmasiddha K. Augsten S. Auricchio A.D. Auriol V.A. Austrup G. Avolio K. Axiotis G. Azuelos D. Babal H. Bachacou K. Bachas A. Bachiu F. Backman A. Badea P. Bagnaia M. Bahmani A.J. Bailey V.R. Bailey J.T. Baines L. Baines O.K. Baker E. Bakos D. Bakshi Gupta V. Balakrishnan R. Balasubramanian E.M. Baldin P. Balek E. Ballabene F. Balli L.M. Baltes W.K. Balunas J. Balz E. Banas M. Bandieramonte A. Bandyopadhyay S. Bansal L. Barak M. Barakat E.L. Barberio D. Barberis M. Barbero M.Z. Barel K.N. Barends T. Barillari M-S. Barisits T. Barklow P. Baron D.A. Baron Mo The ATLAS Collaboration

The identification of jets originating from quarks and gluons,often referred to as quark/gluon tagging,plays an important role in various analyses performed at the Large Hadron Collider,as Standard Model measurements and searches for new particles decaying to quarks often rely on suppressing a large gluon-induced background.This paper describes the measurement of the efficiencies of quark/gluon taggers developed within the ATLAS Collaboration,using √s=13 TeV proton–proton collision data with an integrated luminosity of 140 fb^(-1) collected by the ATLAS experiment.Two taggers with high performances in rejecting jets from gluon over jets from quarks are studied:one tagger is based on requirements on the number of inner-detector tracks associated with the jet,and the other combines several jet substructure observables using a boosted decision tree.A method is established to determine the quark/gluon fraction in data,by using quark/gluon-enriched subsamples defined by the jet pseudorapidity.Differences in tagging efficiency between data and simulation are provided for jets with transverse momentum between 500 GeV and 2 TeV and for multiple tagger working points.

关键词： ATLAS JET QUARK GLUON TAGGING

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