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Recurrent headaches may be caused by cerebral toxoplasmosis

World Journal of Clinical Pediatrics 2014年第3期3卷 59-68页

作者： Joseph Prandota Anna Gryglas Aleksander Fuglewicz agata Zesawska-Faleńczyk Barbara Ujma-Czapska Leszek Szenborn Janusz Mierzwa Department of Social Pediatrics Faculty of Health Sciences Wroclaw Medical University 51618 Wroclaw Poland Department of Pediatric Neurology Taddeus Marciniak Specialistic Hospital 50420 Wroclaw Poland Department of Pediatrics and Infectious Diseases Wroclaw Medical University 50345 Wroclaw Poland Department of Neurosurgery Wroclaw Medical University 50420 Wroclaw Poland

AIM: To establish seroprevalence and provide characteristics of Toxoplasma gondii(TG) infection in children with recurrent headaches. METHODS: The study was performed in 178 children aged 7-17 years admitted consecutively to the Department of Pediatric Neurology from November 2009 to July 2011. The children were surveyed with a questionnaire with the help and assistance of their parents and blood samples taken on admission were studied for the presence of specific anti-TG Ig M, Ig G antibodies and Ig G avidity using enzyme immunoassay Platelia Toxo Ig M, Ig G. RESULTS: The study showed that 19 children(8 boys, 11 girls; 8-17 years old, mean age 14.36 years) hadhigh serum anti-TG Ig G antibody levels(range: 32.2 > 240 UI/m L, mean 120.18 UI/m L; positive value for Ig G was ≥ 9 UI/m L). The avidity index(AI) ranged from 0.202 to 0.925(scale: ≥ 0.5 high AI). The results for Ig M antibodies were all negative and the obtained results ranged from 0.113 to 0.25 U/m L(mean = 0.191 IU/m L) and all values below 0.8 IU/m L were considered negative. The most frequent complaints found in the seropositive patients were headaches that affected the frontal(13 children), occipital(4) and parietal areas(5). Headaches usually had a pulsating(in 7 patients) and squeezing(6) character and rarely were piercing, dull or expanding. Interestingly, 8 children did not feel discomfort during the headaches, probably because they did not have sufficiently increased intracranial pressure yet. The headaches usually appeared 1-2 times/mo, lasted for 2-6 h, and had a mean intensity of 5.5 points in a 10 point subjective scale. The comorbidities included epilepsy(5 patients), various infections in 3 children(chronic eustachitis, chronic rhinitis, chronic purulent tonsillitis, streptococcal pharyngitis, meningitis, allergic diseases), disturbances of behavior, deficits of attention, and ocular and motor concentration disorders in 1 child. The electroencephalographic and neuroimaging studies performed

关键词： Recurrent headaches Children Seroprevalence of anti-Toxoplasma gondii IgG antibodies IgG avidity Chronic Toxoplasma gondii infection Cerebral toxoplasmosis

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非开挖铺管技术方法选择的优化研究

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非开挖技术 2008年第6期25卷 8-11页

作者： agata Zwierzchowska 田建东（译）

关键词：非开挖铺管优化数学模型

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A DL-4- and TNFα-based culture system to generate high numbers of nonmodified or genetically modified immunotherapeutic human T-lymphoid progenitors

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Cellular & Molecular Immunology 2021年第7期18卷 1662-1676页

作者： Ranjita Devi Moirangthem Kuiying Ma Sabrina Lizot Anne Cordesse Juliette Olivré Corinne de Chappedelaine Akshay Joshi agata Cieslak John Tchen Nicolas Cagnard Vahid Asnafi Antonio Rausell Laura Simons Julien Zuber Tom Taghon Frank J.T.Staal Françoise Pflumio Emmanuelle Six Marina Cavazzana Chantal Lagresle-Peyrou Tayebeh Soheili Isabelle André Universitéde Paris Imagine InstituteLaboratory of Human LymphohematopoiesisINSERM UMR 1163ParisFrance Laboratory of Onco-Hematology AP-HPHôpital Necker-Enfants Malades.ParisFrance Universitéde Paris Institut Necker-Enfants Malades(INEM)INSERM UMR 1151ParisFrance Plateforme Bio-informatique UniversitéParis DescartesStructure Fédérative de Recherche NeckerINSERM US24/CNRS UMS 3633ParisFrance Universitéde Paris Imagine InstituteLaboratory of Clinical BioinformaticsINSERM UMR 1163ParisFrance Department of Biotherapy Clinical Investigation Center AP-HPHôpital Necker-Enfants MaladesParisFrance Department of Adult Kidney Transplantation AP-HPHôpital NeckerParisFrance Cancer Research Institute Ghent(CRIG) GhentBelgium Department of Diagnostic Sciences Faculty of Medicine and Health SciencesGhent UniversityGhentBelgium Department of Immunohematology&Blood Transfusion Leiden University Medical CenterLeidenthe Netherlands Team Niche and Cancer in Hematopoiesis Universitéde Paris and UniversitéParis-SaclayINSERMiRCM/IBFJ CEAUMR StabilitéGénétique Cellules Souches et RadiationsFontenay-aux-RosesFrance

Several obstacles to the production,expansion and genetic modification of immunotherapeutic T cells in vitro have restricted the widespread use of T-cell *** the context of HSCT,delayed naïve T-cell recovery contributes to poor outcomes.A novel approach to overcome the major limitations of both T-cell immunotherapy and HSCT would be to transplant human T-lymphoid progenitors(HTLPs),allowing reconstitution of a fully functional naïve T-cell pool in the patient ***,it is challenging to produce HTLPs in the high numbers required to meet clinical ***,we found that adding tumor necrosis factor alpha(TNFα)to a DL-4-based culture system led to the generation of a large number of nonmodified or genetically modified HTLPs possessing highly efficient in vitro and in vivo T-cell potential from either CB HSPCs or mPB HSPCs through accelerated T-cell differentiation and enhanced HTLP cell cycling and *** study provides a clinically suitable cell culture platform to generate high numbers of clinically potent nonmodified or genetically modified HTLPs for accelerating immune recovery after HSCT and for T-cell-based immunotherapy(including CAR T-cell therapy).

关键词： Human T-lymphoid progenitor Tumor necrosis factor alpha Delta-like ligand 4 Hematopoietic stem and progenitor cells Mobilized peripheral blood

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Mutations of TP53 Gene and Oxidative Stress in Alzheimer’s Disease Patients

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Advances in Alzheimer's Disease 2014年第1期3卷 24-32页

作者： Jolanta Dorszewska agata Rozycka Anna Oczkowska Jolanta Florczak-Wyspianska Michal Prendecki Mateusz Dezor Izabela Postrach Pawel P.Jagodzinski Wojciech Kozubski Laboratory of Neurobiology Department of NeurologyPoznan University of Medical SciencesPoznanPoland Department of Biochemistry and Molecular Biology Poznan University of Medical SciencesPoznanPoland Chair and Department of Neurology Poznan University of Medical Sciences10Fredry Str.61-701 PoznanPoland

Alzheimer’s disease (AD) leads to the generation of β-amyloid (Aβ), which may damage DNA and thus lead to apoptosis induction by the p53 pathway. Dysfunction of the p53 protein may then be connected with the development of AD. Studies were conducted on 28 AD patients and 30 non-AD controls. Analysis of TP53 mutations in exon 7 was performed on DNA isolated from whole blood and biochemical parameters in the peripheral lymphocytes of these individuals. Our study showed a silent mutation TP53 C708T (21%) [p TP53 C748A (4%) only in the AD patients. Moreover, in AD patients with the TP53 C748A mutation, the level of 8-oxo-2’- deoxyguanosine (8-oxo2dG) was more than 5 times higher than the average level in this study group. In AD patients with the wild-type TP53 gene, the level of 8-oxo2dG was correlated with the level of protein p53 (R = +0.7388, p TP53 (p TP53 (C748A, C708T) may be associated with pathogenesis of AD.

关键词： TP53 8-oxo2dG p53 OGG1 AD

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刺子莞属（Rhynchospora）中C4植物的发现及其在莎草科（Cyperaceae）花环型解剖中的意义

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海南大学学报(自然科学版) 1986年第2期 67-74页

作者： TOMOSHIRO TAKEDA OSAMU UENO WAICHI agata 廖衍伦

经研究发现刺子莞（Rhynchospora rubra）具有低的CO2补赏点、高的δ18C值,叶解剖为花环型,维管束鞘中存在淀粉粒以及狭窄的脉间距离。以上这些特点说明刺子莞是一种C4植物。更深入的解剖研究,揭示了刺子莞属另外7个种可能也具有C4光合... 详细信息

经研究发现刺子莞（Rhynchospora rubra）具有低的CO2补赏点、高的δ18C值,叶解剖为花环型,维管束鞘中存在淀粉粒以及狭窄的脉间距离。以上这些特点说明刺子莞是一种C4植物。更深入的解剖研究,揭示了刺子莞属另外7个种可能也具有C4光合途径。所以,在莎草科中C4植物不仅发生在藨草族和莎草族中,而且,也发生在刺子莞族中。刺子莞属中的C4植物叶解剖为正常的花环型。但在莎草属（Cyperus）和飘拂草属（Fimbristylis）现在已知的C4植物中,则具有不正常的花环型,即无叶绿体的束内输导组织鞘是插在花环组织和其他绿色组织之间。而刺子莞属的花环组织是直接与其他绿色组织相连,因而提出花环组织与束内输导组织鞘可能是同源的。

关键词：植物莎草科颖花目 C4 Cyperaceae Rhynchospora 刺子莞

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绿豆叶面积估算

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园艺与种苗 1991年第6期 52-53页

作者： A.Hamid W.agata 王立秋

叶面积是一个与产量和干物质生产生理过程有关的重要组成成分。为评价作物生长和发育,常常测量叶面积。各种各样的方法对测量叶面积都是有效的(Marshall,1968),但最常用的方法需破坏植物的样本,而植物样本往往由于小区或群体太小而难以... 详细信息

叶面积是一个与产量和干物质生产生理过程有关的重要组成成分。为评价作物生长和发育,常常测量叶面积。各种各样的方法对测量叶面积都是有效的(Marshall,1968),但最常用的方法需破坏植物的样本,而植物样本往往由于小区或群体太小而难以满足。另一方面,使用叶片扫描的方法进行间接测定,费时费力。

关键词：叶面积小叶最大宽度长度生叶方程绿豆品种

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几何趣味

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现代装饰 2017年第9期 178-179页

作者： Przemyslaw Nowak Lech Moczuski agata Krajewska 彭佳波兰Mili Mlodzi Ludzie设计工作室不详

这座顶层公寓位于波兰,其原本的空间布局极不合理,起居室面积过小,而且最重要的是公寓最大的优点——两面巨大的窗户没有得到完全利用。公寓位于建筑物的顶层,因此视野非常广阔,可以俯瞰波兰的老城区和瓦尔塔河。针对这一特点,设计师的... 详细信息

这座顶层公寓位于波兰,其原本的空间布局极不合理,起居室面积过小,而且最重要的是公寓最大的优点——两面巨大的窗户没有得到完全利用。公寓位于建筑物的顶层,因此视野非常广阔,可以俯瞰波兰的老城区和瓦尔塔河。针对这一特点,设计师的首要想法就是对空间进行重新布局,让住户一进门就能看到优美的景色。

关键词：空间布局瓦尔中间位置几何元素橡木空间的

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The Transcriptional Activity of LAMP3 Gene Involved in Autophagocytosis in Colorectal Cancer LAMP3 Expression in Colorectal Cancer

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Journal of Biosciences and Medicines 2017年第12期5卷 24-36页

作者： Martyna Bednarczyk Malgorzata Muc-Wierzgon Nikola Zmarzly Celina Kruszniewska-Rajs Beniamin Grabarek agata Kazmierczak Bartlomiej Skowronek Klaudia Simka Dariusz Waniczek Katarzyna Walkiewicz Urszula Mazurek Department of Internal Medicine School of Public Health in BytomMedical University of SilesiaKatowicePoland Department of Molecular Biology School of Pharmacy with the Division of Laboratory Medicine in SosnowiecMedical University of SilesiaKatowicePoland Chair of General Colorectal and Polytrauma SurgeryDepartment of Propaedeutics SurgeryMedical University of SilesiaKatowicePoland

Background: Autophagocytosis is a biological process involving the controlled distribution of cell fragments and organelles in order to obtain an additional source of energy. LAMP3 (lysosome-associated membrane protein 3) is a heavily glycosylated integral membrane protein located mainly in the lysosomal membrane. Recent studies have shown that it participates in tumor metastasis and drug resistance. Its main role is contribution to tumor cells proliferation, migration and invasion. The aim of this study was to determine changes in transcriptional activity of LAMP3 at various stages of colorectal cancer. In addition, an attempt was made to select miRNAs potentially regulating LAMP3 expression using bioinformatic databases. Methods: The study was conducted on healthy colon samples and colon cancer samples in four stages. Molecular analysis included the extraction of total RNA, purification of the obtained extracts, expression profile analysis using oligonucleotide microarray technique and in silico determination of miRNAs potentially regulating the LAMP3 expression. Results: The level of LAMP3 expression is higher in colorectal adenocarcinoma cells than in non-cancerous cells and depends on the stage of the disease. Conclusions: LAMP3 may promote cancer progression, metastasis and cause the resistance to treatment.

关键词： Autophagy Colorectal Cancer LAMP3 Microarrays miRNA

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Insulin-like growth factor-1 mRNA isoforms and insulinlike growth factor-1 receptor mRNA expression in chronic hepatitis C

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World Journal of Gastroenterology 2015年第13期21卷 3867-3875页

作者： Aldona Kasprzak Agnieszka Adamek Wieslawa Przybyszewska Przemyslaw Pyda Jacek Szmeja Agnieszka Seraszek-Jaros agata Lanzafame Anna Surdacka Iwona Mozer-Lisewska Maria Koczorowska Department of Histology and Embryology Poznan University of Medical Sciences60-781 PoznanPoland Department of Infectious Diseases Poznan University of Medical Sciences61-285 PoznanPoland Department of General Surgery Gastroenterological Oncology and Plastic SurgeryPoznan University of Medical Sciences60-355 PoznanPoland Department of Bioinformatics and Computational Biology Chair of Clinical PathomorphologyPoznan University of Medical Sciences60-529 PoznanPoland Department and Clinics of Conservative Dentistry and Periodontology Poznan University of Medical Sciences60-812PoznanPoland Department of Molecular Virology Adam Mickiewicz University61-614 PoznanPoland

AIM: to evaluate the expression of different insulinlike growth factor(IGF)-1 mRNA isoforms and IGF-1 receptor(IGF-1R) mRNA in hepatitis C virus(HCV)-infected livers. METHODS: Thirty-four liver biopsy specimens from chronic hepatitis C(CH-C) patients were obtained before anti-viral therapy. Inflammatory activity(grading) and advancement of fibrosis(staging) were evaluated using a modified point scale of METAVIR. The samples were analyzed using quantitative real-time PCR technique. From fragments of liver biopsies and control liver that were divided and ground in liquid nitrogen, RNA was isolated using RNeasy Fibrous Tissue Mini Kit according to the manufacturer's instruction. Expression levels of IGF-1 mRNA isoforms(IGF-1A, IGF-1B, IGF-1C, P1, and P2) and IGF-1R mRNA were determined through normalization of copy numbers in samples as related to reference genes: glyceraldehyde-3-phosphate dehydrogenase and hydroxymethylbilane synthase. Results on liver expression of the IGF-1 mRNA isoforms and IGF-1R transcript were compared to histological alterations in liver biopsies and with selected clinical data in the patients. Statistical analysis was performed using Statistica PL v. 9 software. RESULTS: The study showed differences in quantitative expression of IGF-1 mRNA variants in HCV-infected livers, as compared to the control. Higher relative expression of total IGF-1 mRNA and of IGF-1 mRNAs isoforms(P1, A, and C) in HCV-infected livers as compared to the control were detected. Within both groups, expression of the IGF-1A mRNA isoform significantly prevailed over expressions of B and C isoforms. Expression of P1 mRNA was higher than that of P2 only in CH-C. Very high positive correlations were detected between reciprocal expressions of IGF-1 mRNA isoforms P1 and P2(r = 0.876). Expression of P1 and P2 mRNA correlated with IGF-1A mRNA(r = 0.891; r = 0.821, respectively), with IGF-1B mRNA(r = 0.854; r = 0.813, respectively), and with IGF-1C mRNA(r = 0.839; r = 0.741, respe

关键词： Chronic hepatitis C Insulin-like growth factor-1 receptor Insulin-like growth factor-1 mRNA isoforms Quantitative polymerase chain reaction

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Chondrichthyes Chitinase: Molecular Cloning, Distribution, and Phylogenetic Analysis

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Open Journal of Marine Science 2018年第1期8卷 136-151页

作者： Miku Watanabe Hiromi Kakizaki Momo Kanai Satoshi Kawashima Kaneyuki Hamaguchi Hiroki Mizuno Tsubasa Ueno Chiaki Yasukawa Ryuji agata Mana Ikeda Hideto Fukushima Mitsuhiro Ueda Masahiro Matsumiya College of Bioresource Sciences Nihon University Fujisawa Japan Laboratory of Biocycle Engineering Department of Applied Biological Chemistry Osaka Prefecture University Sakai Japan

We have previously reported the presence of three types of chitinase (acidic fish chitinase-1: AFCase-1, acidic fish chitinase-2: AFCase-2, fish chitinase-3: FCase-3) in Actinopterygii. In the present research, we report the identification of the novel chitinase genes HjChi (ORF: 1380 bp) and DkChi (ORF: 1440 bp) from the stomach of Chondrichthyes, Japanese bullhead shark (Heterodontus japonicas) and Kwangtung skate (Dipturus kwangtungensis), respectively. Organ-specific expression analysis identified the stomach-specific expression of HjChi, whereas DkChi was expressed widely in all organs. Chitinase activity was measured using pNP-(GlcNAc)n (n = 2, 3) as a substrate and β-N-acetylhexosaminidase (Hex) activity was measured using pNPGlcNAc. Relatively high values of chitinase activity were observed in the stomach, spleen, and gonads of the Japanese bullhead shark, H. japonicas , compared with that observed in the stomach of the Kwangtung skate D. kwangtungensis . However, Hex activity was detected throughout the body of both species. The optimal pH of chitinase in both the Japanese bullhead shark, H. japonicas, and the Kwangtung skate, D. kwangtungensis, were 3.5 - 5.5 and 3.5 - 4.0, respectively, and 4.0 for Hex in both species. Phylogenetic analysis revealed that Chondrichthyes chitinase forms a unique group (Chondrichthyes chitinase). These results suggested that the possibility of the formation of chitinase groups for each class in the phylogenetic analysis based on the observation of class-specific chitinase.

关键词： Chitinase Distribution Chondrichthyes cDNA Cloning Phylogenetic Analysis

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