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ORIGINAL ARTICLES APPLICATION OF SILVER ACETATE AUTOMETALLOGRAPHY AND GOLD-SILVER STAINING METHODS FOR IN SITU DNA HYBRIDIZATION

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中华医学杂志(英文版) 1993年第2期106卷 83-92页

作者： GW Hacker ah Graf C Hauser-Kronberger G Wirnsberger A Schiechl G Bernatzky U Wittauer 苏慧慈 H Adam j.Thurner G Danscher L Grimelius Landeskrankenanstalten Salzburg Institute of Pathological AnatomyImmunohistochemistry and Biochemistry UnitSalzburgAustria University of Graz Institute of PatholOgical AnatomyGraz.Austria University of Salzburg Institute of ZoologySalzburgAuatria University of Salzburg Institute of ZoologySalzburgAustria Department of Histology and Embryology Fourth Military Medical UniversityXi'anShaanxiPR China University of Salzburg Institute of ZoologySalzburgAuStria Landeskrankenanstalten Salzburg Institute Of Pathological AnatomyyImmunohistochemistry and Biochemistry UnitSalzburgAustria University of Aarhus Institute of AnatomyDepartment of NeurobiologyAarhusDenmark Uppsala University Hospital Institute of PathologyUppsalaSweden

In situ hybridization using biotinylated DNA probes hasbecome an important tool in *** is well knownthat the sensitivity of the methods used to demonstrate viralDNA in formalin-fixed and paraffin-embedded specimen de-pends strongly on the detection system *** the presentstudy,an optimized in situ DNA hybridization protocol wascombined with four different approaches of gold-silver *** silver enhancement,the recently described meth-od of silver acetate autometallography,a technique allowinghighly efficient development without the necessity of dark roomillumination has been *** most efficient detection methodfound in our experiments was the use of gold-adsorbedanti-biotin antibodies with subsequent silver *** procedure can be completed in 5 hours includinghybridization and is a highly sensitive alternative to peroxidaseand alkaline phosphatase detection systems.

关键词： MESSENGER-RNA ELECTRON-MICROSCOPY PARafFIN SECTIONS DETECTION SYSTEMS GENE-EXPRESSION CDNA PROBE VIRUS-DNA IMMUNOGOLD TISSUE IMMUNOCYTOCHEMISTRY

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Vocal repertoire of the New Zealand kea parrot Nestor notabilis

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Current Zoology 2012年第5期58卷 727-740页

作者： Raoul SCHWING Stuart PARSONS Ximena j. NELSON School of Biological Sciences University of Auckland Private Bag 92019 Auckland New Zealand School of Biological Sciences University of Canterbury Private Bag 4800 Christchurch New Zealand

The unique alpine-living kea parrot Nestor notabilis has been the focus of numerous cognitive studies, but its com- munication system has so far been largely neglected. We examined 2,884 calls recorded in New Zealand＇s Southern Alps. Based on audio and visual spectrographic differences, these calls were categorised into seven distinct call types： the non-oscillating ＇screech＇ contact call and ＇mew＇; and the oscillating ＇trill＇, ＇chatter＇, ＇warble＇ and ＇whistle＇; and a hybrid ＇screech-trill＇. Most of these calls contained aspects that were individually unique, in addition to potentially encoding for an individual＇s sex and age. Additionally, for each recording, the sender＇s previous and next calls were noted, as well as any response given by conspecifics. We found that the previous and next calls made by the sender were most often of the same type, and that the next most likely pre- ceding and/or following call type was the screech call, a contact call which sounds like the ＇kee-ah＇ from which the bird＇s name derives. As a social bird capable of covering large distances over visually obstructive terrain, long distance contact calls may be of considerable importance for social cohesion. Contact calls allow kea to locate conspecifics and congregate in temporary groups for social activities. The most likely response to any given call was a screech, usually followed by the same type of call as the ini- tial call made by the sender, although responses differed depending on the age of the caller. The exception was the warble, the kea＇s play call, to which the most likely response was another warble. Being the most common call type, as well as the default response to another call, it appears that the ＇contagious＇ screech contact call plays a central role in kea vocal communication and social cohesion [Current Zoology 58 （5）： 727-740, 2012].

关键词： Acoustic signals Communication Kea Psittacidae Social behaviour Habitat

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Effects of feeding a diet containing Gymnema sylvestre extract: attenuating progression of obesity in C57BL/6j.mice

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Asian Pacific j.urnal of Tropical Medicine 2016年第5期9卷 425-431页

作者： Hyeon-j.ong Kim Seong-Ho Hong Seung-Hee Chang Sanghwa Kim ah Young Lee Yoonj.ong j.ng Orkhonselenge Davaadamdin Kyeong-Nam Yu j.-Eun Kim Myung-Haing Cho Laboratory of Toxicology College of Veterinary Medicine Seoul National University Graduate Group of Tumor Biology Seoul National University Graduate School of Convergence Science and Technology Seoul National University Advanced Institutes of Convergence Technology Seoul National University Institute of Green Bio Science Technology Seoul National University

Obj.ctive: To investigate the effect of Gymnema sylvestre extract(GS) on initial anti-obesity, liver inj.ry, and glucose homeostasis induced by a high-fat diet(HFD). Methods: The dry powder of GS was extracted with methanol, and gymnemic acid was identified by high performance liquid chromatography as deacyl gymnemic acid. Male C57BL/6j.mice that fed on either a normal diet, normal diet containing 1 g/kg GS(CON+GS) HFD, or HFD containing 1.0 g/kg GS(HFD+GS) for 4 weeks were used to test the initial anti-obesity effect of GS. Body weight gain and food intake, and serum levels about lipid and liver inj.ry markers were measured. Histopathology of adipose tissue and liver stained with hematoxylin and eosin(H&E) and oil-red O were analyzed. after 4 weeks of GS extract feeding, intraperitoneal glucose tolerance test(IPGTT) was performed. Results: The methanol extracts of GS exerted significant anti-obesity effects in HFD+GS group. They decreased body weight gain, a lower food and energy efficiency ratio, and showed lower serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein(LDL)-cholesterol, very-low density lipoprotein(VLDL)-cholesterol and leptin compared with the HFD group. The decreases of abdominal as well as epididymal fat weight and adipocyte hypertrophy, lipid droplets in liver, and serum levels of aspartate aminotransferase(AST) and alanine transaminase(ALT) were also observed. The CON+GS group showed an effect of glucose homeostasis compared to the CON group. Conclusions: This study shows that GS provide the possibility as a key role in an initial anti-obesity effects feeding with a HFD.

关键词： Gymnema sylvestre extract Obesity Adipocyte Body weight gain High-fat diet Liver inj.ry

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颏孔与颏神经之方向在进化中的变化

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解剖学报 1953年第1期 78-81页

作者：张查理西北医学院解剖科

1.一切下等动物之颏孔的方向都朝前上,或一直朝前.婴儿颏孔之方向与下等动物相似。2.婴儿时代以後,颏孔之方向乃转向上後,与下等动物完全不同。3.颏神经初出颏孔时之行路的方向,在婴儿时代,是朝前上与下等动物相同,6岁以後则转向上後,... 详细信息

1.一切下等动物之颏孔的方向都朝前上,或一直朝前.婴儿颏孔之方向与下等动物相似。2.婴儿时代以後,颏孔之方向乃转向上後,与下等动物完全不同。3.颏神经初出颏孔时之行路的方向,在婴儿时代,是朝前上与下等动物相同,6岁以後则转向上後,而异於禽兽矣。4.颏孔与颏神经之方向的变更具有进化适应的意义。

关键词：颏神经颏孔下颏骨

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DECENTRALIZED CONTROL FOR NONLINEAR DYNAMICAL SYSTEMS: AN L_2-GAIN CONTROL APPROACH

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Chinese j.urnal of Aeronautics 1997年第2期10卷 135-143页

作者： Gong Cheng(Department of Eletronic Engineering, Northwestern PolytechnicalUniversity, Xi’an, 710072, China)S. Thompson(Department of Mechanical and Manufacturing Engineering, The Queen’s Universityof Belfast, Belfast BT9 5ah, United Kingdom)Dai Guanzhong( Department of Electronic Engineering NW Polytechnical University Xi'an 710072 China Dept. of Mech. and Mfg. Engineering Queen's University of Belfast Belfast BT9 5Ah United Kingdom Dept. of Automat. Contr. Engineering NW Polytechnical University Xi'an 710072 China

A control method is presented for the problem of decentralized stabilizationof large scale nonlinear systems by designing robust controllers, in the sense of L2-gaincontrol, for each subsystem. An uncertainty tolerance matrix is defined to characterize thedesired robustness leve1 of the overall system. It is then identified that, for a given uncer-tainty tolerance matrix, the design problem is related to the existence of a smooth Positivedefinite solution to a modified Ham ilton -j.cobi - Bellman (H-j.B ) equa tion. The solution,if exists, is exactly the payoff function in terms of the game theory. A decentralized statefeedback law is duly designed, which, under the weak assumption of the zero-state ob-servability on the system, renders the overall closed-loop system aspoptotically stable withan explicitly expressed stability region. Finally, relation between the payoff function andthe uncertainty tolerance matrix is provided, highlighting the 'knowing less and payingmore' philosophy.

关键词： nonlinear systems dynamical systems control Hamilton-j.cobi-Bellman equation

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Neuronal inj.ry and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia

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Neural Regeneration Research 2017年第12期12卷 2007-2013页

作者： Hyun-j.n Tae Il j.n Kang Tae-Kyeong Lee j.ong Hwi Cho j.e-Chul Lee Myoung Cheol Shin Yoon Sung Kim j.n Hwi Cho j.ng-Dai Kim j. Hyeon ahn j.on Ha Park In-Shik Kim Hyang-ah Lee Yang Hee Kim Moo-Ho Won Young j.o Lee Bio-Safety Research Institute College of Veterinary Medicine Chonbuk National University Iksan South Korea Department of Food Science and Nutrition Hallym University Chuncheon South Korea Department of Neurobiology School of Medicine Kangwon National University Chuncheon South Korea Department of Emergency Medicine School of Medicine Kangwon National University Chuncheon South Korea Department of Emergency Medicine Samcheok Medical Center Samcheok South Korea Division of Food Biotechnology School of Biotechnology Kangwon National University Chuncheon South Korea Department of Biomedical Science Research Institute of Bioscience and Biotechnology Hallym University Chuncheon South Korea Department of Obstetrics and Gynecology School of Medicine Kangwon National University Chuncheon South Korea Department of Surgery School of Medicine Kangwon National University Chuncheon South Korea Department of Emergency Medicine Seoul Hospital College of Medicine Sooncheonhyang University Seoul South Korea

Low survival rate occurs in patients who initially experience a spontaneous return of circulation after cardiac arrest（CA）. In this study, we induced asphyxial CA in adult male Sprague-Daley rats, maintained their body temperature at 37 ± 0.5°C, and then observed the survival rate during the post-resuscitation phase. We examined neuronal damage in the hippocampus using cresyl violet（CV） and Fluore-j.de B（F-j.B） staining, and pro-inflammatory response using ionized calcium-binding adapter molecule 1（Iba-1）, glial fibrillary acidic protein（GFAP）, and tumor necrosis factor-alpha（TNF-α） immunohistochemistry in the hippocampus after asphyxial CA in rats under normothermia. Our results show that the survival rate decreased gradually post-CA（about 63% at 6 hours, 37% at 1 day, and 8% at 2 days post-CA）. Rats were sacrificed at these points in time post-CA, and no neuronal damage was found in the hippocampus until 1 day post-CA. However, some neurons in the stratum pyramidale of the CA region in the hippocampus were dead 2 days post-CA. Iba-1 immunoreactive microglia in the CA1 region did not change until 1 day postCA, and they were activated（enlarged cell bodies with short and thicken processes） in all layers 2 days postCA. Meanwhile, GFAP-immunoreactive astrocytes did not change significantly until 2 days post-CA. TNF-α immunoreactivity decreased significantly in neurons of the stratum pyramidale in the CA1 region 6 hours post-CA, decreased gradually until 1 day post-CA, and increased significantly again 2 days post-CA. These findings suggest that low survival rate of normothermic rats in the early period of asphyxia-induced CA is related to increased TNF-α immunoreactivity, but not to neuronal damage in the hippocampal CA1 region.

关键词： nerve regeneration post-cardiac arrest syndrome normothermia neuronal damage gliosis tumor necrosis factor-alpha neural regeneration

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Addressing Low Voltage Network Power Quality Issues through the Implementation of a Microgrid

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j.urnal of Energy and Power Engineering 2011年第9期5卷 870-876页

作者： j. Lava j.F.G. Cobben W.L. Kling F. van Overbeeke Department of Electrical Engineering Eindhoven University of Technology MB Eindhoven 5600 The Netherlands Alliander N. V Utrechtseweg 68 AH Arnhem 6812 The Netherlands EMforce B. V. AD Krimpen aan den ljsse12920 The Netherlands

The results of the implementation of an actual microgrid in the Netherlands are presented. This microgrid has photovoltaic panels as microsources, energy storage, and a flexible AC distribution interfacing system that can operate connected to the public grid or autonomously where it regulates the site＇s voltage and frequency. In this paper, the potential of the microgrid in improving power quality issues of the site, specifically harmonic distortions, is demonstrated. Results show that flexible AC distribution interfacing system devices were able to compensate voltage harmonics when the microgrid was operating connected to the public grid and when operating autonomously. Other tests such as short-circuit, synchronization and blackstart were also conducted. The improvement in power quality and positive results of the other tests demonstrate that a self-supporting, reliable and efficient operation of the microgrid can be achieved.

关键词： Microgrid power quality FACDS harmonic compensation.

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原发性乳腺癌:化疗前和化疗期间固有磁敏感加权成像与组织学相关性研究

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国际医学放射学杂志 2011年第1期34卷 79-80页

作者： N.j.Taylor A.Makris M.L.ah-See M.j.Beresford j.j.Stirling 路红 Academic Oncology Unit Mount Vernon Cancer Centre

目的探讨原发性乳腺癌固有磁敏感加权(ISW)成像与MR动态成像和组织学表现的相关性,并评价化疗前基线横向弛豫率(R2*)、T2*弛豫变化(ΔR2*)与新辅助化疗(NAC)

关键词：乳腺癌磁敏感基线相关性乳腺肿瘤原发性

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动态增强MR成像对新辅助化疗原发性乳腺癌生存期的预测

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国际医学放射学杂志 2011年第5期 472-473页

作者： A.Makris M.j.Beresford N.j.Taylor M.L.W.ah-See j.j.Stirling 刘芳 Academic Oncology Unit Mount Vernon Cancer CentreRickmansworth RdNorthwoodMiddlesexHA6 2RNEngland

目的探讨原发性乳腺癌2个周期新辅助化疗(NAC)后行动态增强MR成像并测量其早期血管参数变化以预测无病生存期和总生存期的可行性。材料与方法本研

关键词：乳腺癌总生存期无病生存乳腺肿瘤动态增强新辅助化疗原发性 MR

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术前检测患者血液中总蛋白、白蛋白与白细胞介素-1受体拮抗剂作为切口疝修补术后是否出现血清肿的预测指标

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中华疝和腹壁外科杂志：电子版 2012年第2期6卷 77-79页

作者： Klink CD Binnebosel M Lucas ah Schachtrupp A Grommes j.Conze j.Klinge U Neumann U j.nge K 宗振（译）陈双（校）不详中山大学附属孙逸仙纪念医院胃肠胰腺外科华南疝外科治疗培训中心广州510120

患者切口疝修补术后出现血清肿是非常常见的,约占20%～30%,其中10%的患者渗液量超过50ml。出现血清肿并不一定出现并发症,但血清肿患者术后容易出现疼痛不适,尤其是补片感染导致修补手术失败,切口疝复发。对某些患者及时使用细针抽液,... 详细信息

患者切口疝修补术后出现血清肿是非常常见的,约占20%～30%,其中10%的患者渗液量超过50ml。出现血清肿并不一定出现并发症,但血清肿患者术后容易出现疼痛不适,尤其是补片感染导致修补手术失败,切口疝复发。对某些患者及时使用细针抽液,甚至是手术治疗都是必要的。

关键词：白细胞介素-1受体拮抗剂修补术后血清肿切口疝患者术前检测预测指标总蛋白

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