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Bacterial Community diversity of a Congolese Traditional Fermented Food, “Pandé”, Revealed by Illumina Miseq^TM Sequencing of 16S rRNA Gene

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Open Journal of Applied Sciences 2022年第3期12卷 387-405页

作者： C. P. Limingui polo A. A. Lebonguy A. E. Lembella Boumba S. A. E. Nzaou M Bokamba Moukala J. Goma-Tchimbakala M. diatewa Institut national de Recherche en Sciences Exactes et Naturelles Brazzaville Congo Faculté des Sciences et Techniques Brazzaville Congo Ecole Nationale Supérieure d’Agronomie et de Foresterie Brazzaville Congo Faculté des Sciences de la Santé Brazzaville Congo

Pandé is a Congolese fermented food produced from fruit pulps of raffia palm. The aim of this study was to explore the diversities in bacterial communities in the unfermented pulps (control) (PNFL) and fermented pulps obtained from fruits using boiling (PMF) and purchased at markets in Brazzaville (PFL) and using Illumina MiSeqTM sequencing of 16S rRNA gene. A total number of 157,054 sequences was obtained and grouped into 737 operational taxonomic units (OTUs). These were classified into 13 phyla, 26 classes, 48 orders, 96 families and 176 genera. At the phylum level, the most frequent bacterial communities were: for PFM, Proteobacteria (38.94%) and Firmicutes (19.37%);for PFL, Firmicutes (69.10%) and Proteobacteria (30.89%);for PNFL, Firmicutes (84.92%) and Proteobacteria (14.22%). were the most frequent in PFL, while Acidobacteria (40.02%), Proteobacteria (38.94%) and Firmicutes (19.37%) were preponderant in PFM. Firmicutes (84.92%) and Proteobacteria (14.22%) were the most common in unfermented pulps (PNFL). Lactobacillus (47.70%) and Gluconobacter genera (15.33%) were the most represented in the PFL sample. Acidipila (35.56%) and Rhizomicrobium (11.41%) were preponderant genera in the PFM sample. Weissella genus was the most frequent in the PNFL sample. Hierarchical cluster indicated that the samples could be grouped into two groups of different origins. PCA showed that main genera were higher in the fermented samples. The diversity was higher in the fermented pulps or Pandé than in the unfermented pulps. Pandé can be a potential source for the isolation of bacteria with biotechnological potential.

关键词： diversity Pandé Bacterial Community Fermented Food Raffia Palm

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Propofol is not so safe for ERCP To the Editor

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Hepatobiliary & Pancreatic diseases International 2006年第2期5卷 314-314页

作者： Francisco J.polo-Romero

The Editor welcomes submissions for possible publication in the Letters to the Editor section that consist of commentary on an article published in the Journal or other relevant issues. Letters commenting on an article published in the Journal will be considered if they are received within 6 weeks of the time the article was published. Authors of the article being commented on will be given an opportunity to offer a timely response to the letter. Authors of letters will be notified that the letter has been received. Unpublished letters cannot be returned.

关键词： ERCP Propofol is not so safe for ERCP To the Editor

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Identification,Structure Analyses and Expression Pattern of the ERF Transcription Factor Family in Coffea arabica

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Journal of Botanical Research 2021年第1期3卷 32-45页

作者： Silvia Graciele Hülse de Souza Tiago B.dos Santos Douglas S.Domingues Anne Bernadac Mondher Bouzayen Luiz F.P.Pereira Giuliano Degrassi Valéria Carpentieri-Pípolo Laboratory of Molecular Biology Universidade Paranaense PO box 87502-210UmuaramaPRBrazil Instituto Agronômico do Paraná Laboratório de Biotecnologia VegetalCP 481PO box 86001-970LondrinaPRBrazil Department of Botany Instituto de BiociênciasUniversidade Estadual PaulistaUNESPRio ClaroSPBrazil Laboratoire de Genomique et Biotechnologie des Fruits UMR 990 INRA/INP-ENSAT Chemin de BorderougePO box 10731326 Castanet Tolosan CedexFrance Empresa Brasileira de Pesquisa Agropecuária Embrapa CaféParque Estação BiológicaBrasíliaDFPO box 70770-901Brazil International Centre for Genetic Engineering and Biotechnology(ICGEB) 1Industrial Biotechnology GroupBuenos AiresRepública Argentina Empresa Brasileira de Pesquisa Agropecuária Embrapa TrigoPO box 99001-970Passo FundoRSBrazil

Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic *** work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain,using the EST-based genomic resource of the Brazilian Coffee Genome *** ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in *** addition to the AP2 domain,other conserved domains were identified,typical of members of each *** in silico analysis and expression profiling showed high levels of expression for libraries derived from tissues of fruits,leaves and flowers as well as for libraries subjected to water *** results suggest the participation of the ERF family genes of *** in distinct biological functions,such as control of development,maturation,and responses to water *** results of this work imply in the selection of promising genes for further functional characterizations that will provide a better understanding of the complex regulatory networks related to plant development and responses to stress,opening up opportunities for coffee breeding programs.

关键词： AP2/ERF Coffee Ethylene Transcription factor

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Genetic distance Estimated by RAPD Markers and Performance of Topcross Hybrids in Popcorn

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American Journal of Plant Sciences 2012年第12期3卷 1666-1673页

作者： Silvia Graciele Hülse de Souza Valéria Carpentieri-Pípolo Deoclécio Domingos Garbúglio Nelson da Silva Fonseca Júnior Claudete de Fátima Ruas Paulo Maurício Ruas Laboratory of Molecular Biology Universidade ParanaenseUmuaramaBrazil Agronomy Department Universidade Estadual de LondrinaLondrinaBrazil Instituto Agronomico do Paraná LondrinaBrazil Biology Department Universidade Estadual de LondrinaLondrinaBrazil

The purpose of this study was to investigate the diversity of 14 popcorn populations and a broad genetic base tester using molecular RAPD markers and to estimate the correlation between the genetic distances and the performance of top-cross hybrids. For the evaluation of populations and hybrids resulting from topcrosses, the reduced model of Gardner was used. A genetic distance matrix was generated based on RAPD markers by Jaccard coefficient, and a dendrogram was constructed. In general, topcrosses performed better than the populations per se and evidenced heterosis occurrence in topcrosses. The trait grain weight is influenced by additive as much as by dominance effects. Genetic associations separated the populations in three groups, and RAPD showed to be a useful tool to determine the extension of genetic diversity in popcorn populations and to place genotypes in distinct heterotic groups. Correlations between genetic divergences, detected by RAPD, and the means observed in the topcross crosses were positive and non-significant for expansion volume, plant height, and female flowering, and were negative for grain weight.

关键词： Zea mays L. Heterotic Groups Molecular Markers Testers Expansion Volume Yield

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双管齐下(“疫苗接种”+“日粮添加SDPP”)控非洲猪瘟

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猪业科学 2023年第10期40卷 18-19页

作者：张振玲(编译) 徐州生物工程职业技术学院动物工程学院江苏徐州221006

毋庸置疑,非洲猪瘟(ASF)对养猪业和全球猪肉贸易的影响巨大。ASF不仅使生猪养殖业者损失巨大,也给生猪养殖相关行业以及所有与猪肉生产相关行业都造成了较为严重的经济损失。尽管全世界都在努力开发针对这种致命猪病的疫苗,但是直到目... 详细信息

毋庸置疑,非洲猪瘟(ASF)对养猪业和全球猪肉贸易的影响巨大。ASF不仅使生猪养殖业者损失巨大,也给生猪养殖相关行业以及所有与猪肉生产相关行业都造成了较为严重的经济损失。尽管全世界都在努力开发针对这种致命猪病的疫苗,但是直到目前为止,安全有效的ASF疫苗仍未成功研制出来。

关键词：非洲猪瘟疫苗接种生猪养殖业猪肉生产养猪业日粮添加猪病 ASF

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polo样激酶1在大鼠心肌细胞分化过程中的表达

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解剖学报 2008年第4期39卷 578-581页

作者：邸菁柏树令张凌志高杰佟浩中国医科大学基础医学院人体解剖学教研室沈阳110001 中国医科大学附属盛京医院急诊科沈阳110004

目的通过检测polo样激酶1(plk1)在大鼠生后不同发育阶段心肌组织中的表达,探讨心肌细胞退出细胞周期的相关机制。方法生后0d、3d、7d、10d、2周和成年健康Wistar大鼠各5只,采用针对横纹肌肌动蛋白和磷酸化组蛋白H3的免疫荧光双标方法,... 详细信息

目的通过检测polo样激酶1(plk1)在大鼠生后不同发育阶段心肌组织中的表达,探讨心肌细胞退出细胞周期的相关机制。方法生后0d、3d、7d、10d、2周和成年健康Wistar大鼠各5只,采用针对横纹肌肌动蛋白和磷酸化组蛋白H3的免疫荧光双标方法,测定各组大鼠心肌细胞有丝分裂指数;用RT-PCR和免疫印迹技术,检测心肌组织plk1 mRNA和蛋白表达的改变。结果大鼠生后0d心肌细胞有丝分裂指数[(0.905±0.087)%]是生后2周[(0.372±0.094)%]的2.4倍(P<0.01);plk1基因和蛋白在大鼠生后表达明显下调,2周后未检测到表达。结论plk1基因的表达下调与大鼠心肌细胞增殖停滞密切相关,在大鼠心肌细胞退出细胞周期的调控机制中发挥重要作用。

关键词： polo样激酶1 心肌细胞细胞周期调控 RT-PCR 免疫印迹法免疫荧光大鼠

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Intrahepatic cholangiocarcinoma: review and update

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Hepatoma Research 2018年第6期4卷 25-40页

作者： Vincenzo Cardinale Maria Consiglia Bragazzi Guido Carpino Sabina di Matteo diletta Overi Lorenzo Nevi Eugenio Gaudio Domenico Alvaro Department of Medico-Surgical Sciences and Biotechnologies Polo PontinoSapienza University of RomeRome 00198Italy Department of Movement Human and Health SciencesDivision of Health SciencesUniversity of Rome“Foro Italico”Rome 00100Italy Department of Medicine and Medical Specialties Sapienza University of RomeRome 00198Italy division of Human Anatomy Department of AnatomicalHistologicalForensic Medicine and Orthopedics SciencesSapienza University of RomeRome 00198Italy

Cholangiocarcinoma (CCA) is a heterogeneous group of malignancies that could develop at any level from the biliary tree. CCA is currently classified into intrahepatic (iCCA), perihilar and distal on the basis of its anatomical location. Of note, these three CCA subtypes have common features but also important inter-tumor and intra-tumor differences that can affect the pathogenesis and outcome. A unique feature of iCCA is that it recognizes as origin tissues, the hepatic parenchyma or large intrahepatic and extrahepatic bile ducts, which are furnished by two distinct stem cell niches, the canals of Hering and the peribiliary glands, respectively. The complexity of iCCA pathogenesis highlights the need of a multidisciplinary, translational and systemic approach to this malignancy. This review will focus on the advances of iCCA epidemiology, histo-morphology, risk factors, molecular pathogenesis, revealing the existence of multiple subsets of iCCA.

关键词： Cholangiocarcinoma classifications inflammation cells of origin stem cells molecular profiling

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要孩子,你准备好了吗?

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成长先锋：女人街 2007年第7期 79-79页

作者： polo

1．脏乱测试在沙发、窗帘上,涂上花生酱。在你的床上放置一条鱼,然后任其等待一个夏天。在你假期回来后,你可以感受会有什么景象发生。2．玩具测试买100套乐高的积木,亦可以大头钉作替代。让你一个朋友,蒙上眼睛,从厨房走到卫生间,随手... 详细信息

1．脏乱测试在沙发、窗帘上,涂上花生酱。在你的床上放置一条鱼,然后任其等待一个夏天。在你假期回来后,你可以感受会有什么景象发生。2．玩具测试买100套乐高的积木,亦可以大头钉作替代。让你一个朋友,蒙上眼睛,从厨房走到卫生间,随手不停地扔撒积木(大头钉)于整个屋子。请你不要尖叫和惊讶,一来这对于你的宝宝是小菜一叠,二来你会吵醒他。

关键词：小布袋生生地透明胶带埃菲尔铁塔卷纸可乐瓶虚心请教抓握

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