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Comparison of Two Protocols of Agar Gel Immunodiffusion(AGID)Used to Diagnose of Equine Infectious Anemia(EIA)

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Open Journal of Veterinary Medicine 2015年第7期5卷 169-174页

作者： Maria do Rosario Oliveira da Cunha Beltrao Cid Aristoteles de Siqueira Alencar adriana soares leite Leonardo Tavares Freitas Julianne Cortizo Gonzalez Vania Lucia de Assis Santana Helio Cordeiro Manso-Filho Department of Veterinary Medicine Federal Rural University of PernambucoRecifeBrazil National Animal and Plant Laboratory Ministry of AgricultureLivestock and Food SupplyRecifeBrazil

The aim of the present study was to compare two protocols of Agar Gel Immunodiffusion (AGID) that can be used to diagnose Equine Infectious Anemia (EIA). Different volumes of reagents and support for agar gel were used, as recommended by the Ministry of Agriculture, Livestock and Food Supply (MAPA) and the World Organization for Animal Health (OIE). Equidae sera samples (n = 1000) were tested using a national commercial kit and different volumes (i.e. 25 μL—MAPA and 50 μL—OIE) of reagents and standard sera. Antigen was used as recommended by MAPA. The results were assessed by two operators, 48 hours after beginning the test. At the end of the experiment, a Kappa index of 1 was obtained by the operators, for the interpretation of the results and for the different volumes of reagents used. Similar results of agreement (Kappa index = 1) were obtained when using slides or Petri dishes as support for the agar gel. In both cases, an almost perfect agreement was achieved, suggesting that the use of different supports does not affect the results. These findings indicate that both protocols (MAPA and OIE) can be used to diagnose EIA.

关键词： Horses Equids EIA Diagnosis AGID Serology

Evaluation of Novel 3D Architectures Based on Knitting Technologies for Engineering Biological Tissues

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Journal of Donghua University(English Edition) 2013年第5期30卷 421-426页

作者： RIBEIRO Viviana Pinto RIBEIRO Ana soares SILVA Carla Joana DURES Nelson Feio BONIFCIO Graa CORRELO Vitor Manuel MARQUES Alexandra Pinto SOUSA Rui Amandi OLIVEIRA Ana leite REIS Rui Luís 3B's Research G roup-Biomaterials Biodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine ICVS /3B's-PT G overnment Associated Laboratory University of Minho CeNTI Centre for Nanotechnology and Smart MaterialsV. N. CITEVE Technological Centre for Textile and Clothing IndustryV. N. CBQF-School of Biotechnology Portuguese Catholic University

Textile-based technologies are considered as potential routes for the production of 3D porous architectures for tissue engineering( TE) applications. We describe the use of two polymers,namely polybutylene succinate( PBS) and silk fibroin(SF) to produce fiber-based finely tuned porous architectures by weft and warp knittings. The obtained knitted constructs are described in terms of their morphology, mechanical properties,swelling ability,degradation behaviour,and cytotoxicity. Each type of polymer fibers allows for the processing of a very reproducible intra-architectural scaffold geometry,with distinct characteristics in terms of the surface physicochemistry,mechanical performance,and degradation capability,which has an impact on the resulting cell behaviour at the surface of the respective biotextiles. Preliminary cytotoxicity screening shows that both materials can support cell adhesion and proliferation. Furthermore, different surface modifications were performed( acid /alkaline treatment, UV radiation,and plasma) for modulating cell behavior. An increase of cell-material interactions were observed,indicating the important role of materials surface in the first hours of culturing. Human adipose-derived stem cells( hASCs) became an emerging possibility for regenerative medicine and tissue replacement therapies. The potential of the recently developed silk-based biotextile structures to promote hASCs adhesion,proliferation,and differentiation is also evaluated. The obtained results validate the developed constructs as viable matrices for TE applications. Given the processing efficacy and versatility of the knitting technology, and the interesting structural and surface properties of the proposed polymer fibers,it is foreseen that our developed systems can be attractive for the functional engineering of tissues such as bone,skin,ligaments or cartilage and also for develop more complex systems for further industrialization of TE products.

关键词： textile-based technologies silk polybutylene succinate(PBS) surface modifications human adipose-derived stem cells(hASCs) tissue engineering(TE)

An aqueous extract of Liu Wei Di Huang Wan alters the labeling of blood constituents with Technetium-99m

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Chinese Science Bulletin 2013年第18期58卷 2061-2065页

作者： MATOS Vernica soares de CARMO Fernanda Santos do DINIZ Claudia leite NASCIMENTO Ana Lucia Rosa PEREIRA Márcia Oliveira FERNANDES Joelma Fonseca de Oliveira SANTOS-FILHO Sebastiao David MORENO Silvana Ramos Farias BERNARDO-FILHO Mario Departamento de Biofísica e Biometria Instituto de Biologia Roberto Alcantara GomesUniversidade do Estado do Rio de Janeiro

The Liu Wei Di Huang Wan is a formula of a traditional Chinese medicine that is used to treat asthma patients and has been shown to have several important properties,such as antioxidant and free radical scavenging *** influence of an extract of Liu Wei Di Huang Wan on the labeling of blood constituents with technetium-99m was *** blood(Wistar rats) was incubated with the extract,stannous chloride and technetium-99m,as sodium *** were centrifuged and aliquots of plasma and blood cells were separated and precipitated with trichloroacetic acid,to obtain soluble and insoluble fractions of the blood *** percentage of radioactivity(%ATI) in all the fractions was *** analysis of the results shows that the extract at the highest concentration used(70 mg/mL) decreased significantly(P<0.05) the %ATI(from 96.48 ± 1.19 to 54.46 ± 7.38) on blood cells compartment,(from 81.11 ± 4.15 to 61.33 ± 4.74) on insoluble fractions of blood cells and(from 65.91 ± 2.44 to 13.15 ± 3.62) on insoluble fractions of *** conclusion,the results suggest that the substances present on this extract can alter this labeling process,probably due to(i) redox properties(antioxidant and chelator activities) and/or(ii) specific actions in the binding sites where the 99mTc would be bound on the blood *** a consequence,precaution is suggested on the interpretation of the nuclear medicine results from performed with blood constituents labeled with 99m Tc in patients that have undertaken LWDHW,although the current findings were obtained in experimental animal models.

关键词：六味地黄丸血液成分标签水提物 Wistar大鼠氧化还原性能锝实验动物模型

Anti-Candida and anti-Cryptococcus evaluation of 15 non-alkaloidal compounds from Pterogyne nitens

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Asian Pacific Journal of Tropical Biomedicine 2016年第10期6卷 841-845页

作者： Caroline Sprengel Lima Carlos Roberto Polaquini Mariana Bastos dos Santos Fernanda Patricia Gullo Fernanda Sangalli leite Liliane Scorzoni Vanderlan da Silva Bolzani Maria Jose soares Mendes-Giannini Ana Marisa Fusco-Almeida Andréia Alves Rezende Luis Octavio Regasini Laboratory of Green and Medicinal Chemistry Department of Chemistry and Environmental Sciences Institute of Biosciences Letters and Exact Sciences Sao Paulo State University (UNESP) Department of Clinical Analysis School of Pharmaceutical Sciences Sao Paulo State University (UNESP) Department of Organic Chemistry Institute of Chemistry Sao Paulo State University (UNESP) Department of Biology and Animal Sciences Faculty of Engineering Sao Paulo State University (UNESP)

Objective: To evaluate anti-Candida and anti-Cryptococcus activities of 15 nonalkaloidal compounds from Pterogyne nitens Tulasne(Leguminosae), a South American medicinal ***: Compounds were submitted to antifungal assays, using microdilution method described by Clinical and Laboratory Standards Institute document, with minor modifications. Five species of Candida and two species of Cryptococcus, including clinical isolates were screened. Antifungal activity was expressed by minimum inhibitory concentration(MIC). Amphotericin B and fluconazole were used as standard antifungal ***: Among tested compounds, six substances presented fungal growth inhibition(MIC < 31.2 mg/m L) [three flavone derivatives(1–3), a glycosylated flavonol derivative(5)and two phenolic acids(10 and 12)]. Sorbifolin(1), exhibited potent antifungal activity,demonstrating MIC value of 3.90 mg/m L against Candida glabrata ATCC 90030, Cryptococcus gattii 118 and fluconazole-resistant clinical isolate of Cryptococcus neoformans var. grubii. Pedalin(2) and nitensoside B(3), two glycosylated flavone derivatives, were active against Cryptococcus neoformans ATCC 90012(MIC = 7.80 mg/m L).Conclusions: Flavone derivatives from Pterogyne nitens can serve as prototypes for the design and development of innovative anti-Candida and anti-Cryptococcus hits.

关键词： Candida Cryptococcus Antifungal Pterogyne nitens Flavonoid Opportunistic fungi

Actinic Cheilitis: Clinical Characteristics Observed in 75 Patients and a Summary of the Literature of This Often Neglected Premalignant Disorder

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International Journal of Clinical Medicine 2014年第21期5卷 1337-1344页

作者： Ana Maria de Oliveira Miranda Thiago de Miranda Ferrari Taiana Campos leite Karin soares Goncalves Cunha Eliane Pedra Dias Post-Graduate Program in Pathology Medical SchoolFluminense Federal UniversityNiteroiBrazil

Actinic cheilitis can progress to squamous cell carcinoma in 20% of cases. The purpose of this study was to identify the clinical characteristics of 75 patients with actinic cheilitis and to summarise the current literature concerning actinic cheilitis. A total of 75 patients were evaluated over a 3-year period. The medical history, physical examination results and images were collected from each patient. Demographic data were collected and analysed. To review the literature, the relevant data were collected from scientific journals in the last 30 years. The mean age of the patients was 56 years old;42 patients (56%) were female, and 66 patients (88%) were white. Nineteen (25.3%) patients reported at least one symptom, including pain, burning and itching. Sixty-five (86.7%) patients presented actinic cheilitis only in the lower lip. All of the patients reported sun exposure, and 44 (58.6%) patients were exposed for more than 10 years. The main clinical aspects investigated and analysed included dryness (100%), flaking (72%) and white lesions (57.3%). Lip assessment is extremely important in medical and dental care. From the 15 lesions assessed in the presence of actinic cheilitis, the most common clinical characteristics observed were dryness, flaking and white lesions. It is imperative to know the clinical aspects that may be encountered in actinic cheilitis in order to achieve early diagnosis, thus avoiding lip squamous cell carcinoma transformation.

关键词： Actinic Cheilitis Lip Cancer Sun Exposure Premalignant Disorder

Development and implementation of an automated system to aid laboratory diagnosis using image processing

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Journal of Biomedical Science and Engineering 2013年第5期6卷 579-585页

作者： Alvaro Manoel de Souza soares Marco Rogério da Silva Richetto Joao Bosco Goncalves Pedro Paulo leite do Prado Department of Mechanical Engineering University of TaubateTaubateBrasil Department of Electrical Engineering University of TaubateTaubateBrasil

The objective of this work is to provide an automatic system to count white blood cells in a blood smear. To do so an experiment was assembled, composed by a standard microscope with two step motors coupled to its knobs in order to move the microscope in x and y directions and a web cam which was mounted in the top of the microscope responsible for to acquire images from the smear. The step motors and the web cam are controlled by a microcomputer PC standard via software developed inDelphi. The motors use the parallel port to communicate with the PC and the camera use the USB port. The main idea is to set an initial point into the smear and the automated system will carry over the smear acquiring images (frames with 640 × 480 pixels) and counting the white blood cells encountered. The double histogram threshold technique is implemented to initially exclude the red cells from the image leaving only the white ones. Preliminaries results are obtained and show that the system is quite fast and has a good capacity of selection, even when different kinds of smear are used.

关键词： Image Processing Robotics Automation Pattern Recognition

Water availability drives gradients of tree diversity,structure and functional traits in the Atlantic-Cerrado-Caatinga transition,Brazil

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Journal of Plant Ecology 2018年第6期11卷 803-814页

作者： Marcela de Castro Nunes Santos Terra Rubens Manoel dos Santos Jamir Afonso do Prado Júnior José Marcio de Mello José Roberto soares Scolforo Marco Aurélio leite Fontes Ivan Schiavini Aliny Aparecida dos Reis Inacio Thomaz Bueno Luiz Fernando Silva Magnago Hans ter Steege Departamento de Ciências Florestais Universidade Federal de LavrasCampus UniversitárioPO Box 3037LavrasMinas Gerais 37200-000Brazil Instituto de Biologia Universidade Federal de UberlândiaCampus Umuarama-Bloco 2DPO Box 593UberlândiaMinas Gerais 38400-902Brazil Applied Ecology and Conservation Lab Programa de Pós‐graduação Ecologia e Conservação da BiodiversidadeUniversidade Estadual de Santa CruzIlhéusBahia 45662-900Brazil Naturalis Biodiversity Center Vondellaan 55Postbus 95172300 RA LeidenThe Netherlands Coordenação de Botânica Museu Paraense Emílio GoeldiAv.Magalhães Barata 376PO Box 399BelémPara´66040-170Brazil

Aims Climate and soil are among the most important factors determin-ing variation in tree communities,but their effects have not been thoroughly elucidated to date for many vegetation *** this study,we evaluate how climate and soil gradients affect gradients of vegetation composition,species diversity and dominance,structure and functional traits(seed mass and wood density)using over 327000 trees in 158 sites distributed along environmental gradients in the transitions among the Atlantic forest,Cerrado and Caatinga in Minas Gerais State(MG),Brazil(nearly 600000 km2).Methods Gradients in species,genus and family abundance in addition to basal area,stem density,species diversity(Fisher’s alpha),domin-ance percentage,seed mass and wood density were correlated using multiple regressions with environmental variables,as summarized in four principal component analysis axes(two climatic-precipi-tation seasonality and temperature range-and two edaphic-soil fertility and soil moisture).Additionally,ordinary kriging maps were used to better illustrate the *** Findings Multiple regression models indicate that all variables but domin-ance percentage were affected by one or more of the environmental gradients,but the average R2 was low(26.25%).Kriging maps rein-forced the patterns observed in the regression *** seasonality and soil moisture gradients were the most important gra-dients affecting vegetation *** finding suggests that water availability is an important determinant of vegetation features in these vegetation transitions.

关键词： climate environmental gradient soil southeastern Brazil vegetation

Infrared Spectroscopy of Titania Sol-Gel Coatings on 316L Stainless Steel

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Materials Sciences and Applications 2011年第10期2卷 1375-1382页

作者： Daniela Cordeiro leite Vasconcelos Vilma Conceicao Costa Eduardo Henrique Martins Nunes Antonio Claret soares Sabioni Massimo Gasparon Wander Luiz Vasconcelos Department of Metallurgical and Materials Engineering Federal University of Minas GeraisBelo HorizonteBrasil Department of Physics Federal University of Ouro PretoOuro PretoBrasil School of Earth Sciences The University of QueenslandSt.LuciaAustralia

Sol-gel titania films were deposited on 316L stainless steel using titanium isopropoxide as a chemical precursor. Dip-coating was performed at withdrawal speeds of 6 mm/min, 30 mm/min, and 60 mm/min. Deposited gel films were heat treated in air at 80℃, 100℃, 300℃, and 400℃. The structural evolution of the coatings was evaluated by infrared reflection-absorption spectroscopy. The influence of the withdrawal speed and the heat treatment temperature on the structure of the films was studied by varying the reflectance incidence angle during the infrared experiments and by Glow Discharge Spectrometry. Free functional groups were detected. The results indicate the formation of bidendate bridging coordination of carboxylic acid to titanium. Titanium atoms can also be pentacoordinated according to the processing conditions of the films. We observed a tendency of increasing amounts of OH groups with decreasing reflectance incidence angle. The film hardness was measured via Knoop microindenation hardness test.

关键词： Films Sol-Gel Infrared Spectroscopy Glow Discharge Spectrometry Knoop Hardness

Evaluation of functional capacity, body composicion and pulmonary function after bariatric surgery

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Health 2013年第8期5卷 47-53页

作者： Flavia Sales leite Marcela Cangussu Barbalho-Moulim Kadma Karenina Damaceno soares Fernanda Dultra Dias Fabiana Sobral Peixoto-Souza Dirceu Costa Uninove—Nove de Julho University Sao PauloBrazil UFSCar—Federal University of Sao Carlos Sao CarlosBrazil

Introduction: Obesity has been considered a global epidemic and a serious public health problem closely related to other diseases. Moreover, obese individuals have limitations in physical capacity and pulmonary function. Weight loss induced by bariatric surgery appears to help reverse this situation. However, questions about physical capacity and lung function, and its relationship with body composition remain to be clarified. Objective: To evaluate the physical capacity, body composition and lung function in women undergoing bariatric surgery. Methods: Twenty five women who underwent bariatric surgery between 6 and 24 months ago, and 10 non-obese women were evaluated for exercise capacity by the six minutes walk test (6 MWT), body composition by bio-electrical impedance analysis and lung function by spirometry and measurement of maximal static respiratory pressures. Results: Subjects with BMI 2 were found to have better physical performance, demonstrated by increased 6 MWT distance, not differing from non-obese women. When compared with non-obese women, there were no significant differences in body composition and lung function. Higher BMI was correlated with shorter distances in 6 MWT. Conclusion: 6 to 24 months after surgery, when the weight loss of patients who underwent bariatric surgery reaches levels considered non-obese, it provides improvement in physical performance to a level similar to that of non-obese patients.

关键词： Obesity Bariatric Surgery Exercise Spirometry Muscle Strength