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Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae Spirulina Strain LEB-18 and Chlorella pyrenoidosa

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Advances in Materials Physics and Chemistry 2014年第1期4卷 6-12页

作者： Leticia Marques de Assis adriana rodrigues machado Amanda de Souza da Motta Jorge Alberto Vieira Costa Leonor Almeida de Souza-Soares School of Chemistry and Food Federal University of Rio GrandeRio GrandeBrazil Federal Institute Sul-Rio-Grandense Campus PelotasVisconde da GracaPelotasBrazil Institute of Basic Health Sciences Federal University of Rio Grande do SulPorto AlegreBrazil

The objective of this study was to elaborate liposomes, through the lipid film hydration methodology, to nanoencapsulate phenolic compounds of Spirulina LEB-18 and Chlorella pyrenoidosa microalgae, and evaluate their physicochemical characteristics and storage stability for 21 days. The total phenolic compounds were evaluated using a calibration curve of gallic acid using methanol and ethanol as extraction solvents. The size and polydispersity index of nanovesicles were determined by light scattering and the percentage encapsulation efficiency was determined by a centrifugation process. The stability of the liposomes at storage time was measured by zeta potential for 21 days. The methanol extracts from Spirulina had a higher content of phenolic compounds (2.62 mg gallic acid·g-1 of microalgae) compared to the extracts of Chlorella. However, liposomes with ethanolic extracts of the two algae showed higher encapsulation efficiency. The value was higher (96.40%) for Chlorella. All samples obtained nanometric size, with the highest value obtained for the liposome containing ethanol extract of Chlorella (239 nm) differing significantly (p ≤ 0.05) from the others. The liposomes containing extracts of Spirulina were more stable during the 21 days of storage, whereas, those consisting of ethanol extract showed no significant difference (p ≤ 0.05) throughout this period.

关键词： Microalgae Phenols Encapsulation Liposome

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Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： Bruna Cristina Pinto adriana Barboza Alves Osania Emerenciano Ferreira Gustavo Henrique Gravatim Costa Magno Andréde Oliveira Augusto Cesar da Silva Bezerra Alan rodrigues Teixeira machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil Department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil Department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil Department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

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Physiology and health assessment,risk balance,and model for endstage liver disease scores:Postoperative outcome of liver transplantation

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World Journal of Transplantation 2025年第1期15卷 86-94页

作者： Raquel Hohenreuther Andresa ThoméSilveira Edison Moraes rodrigues Filho Anderson Garcez Bruna Goularth Lacerda Sabrina Alves Fernandes Claudio Augusto Marroni Postgraduate Program in Hepatology Universidade Federal de Ciências da Saúde de Porto AlegrePorto Alegre 90050-170Brazil Department of Intensive Medicine Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903Brazil Department of Public Health University of Vale do Rio dos SinosSao Leopoldo 93022-750Brazil

BACKGROUND Liver transplantation aims to increase the survival of patients with end-stage liver diseases and improve their quality of *** number of organs available for transplantation is lower than the *** provide fair organ distribution,predictive mortality scores have been *** To compare the Acute Physiology and Chronic Health Evaluation IV(APACHE IV),balance of risk(BAR),and model for end-stage liver disease(MELD)scores as predictors of *** Retrospective cohort study,which included 283 adult patients in the postoperative period of deceased donor liver transplantation from 2014 to *** The transplant recipients were mainly male,with a mean age of 58.1 *** were mostly male,with a mean age of 41.6 *** median cold ischemia time was 3.1 hours,and the median intensive care unit stay was 5 *** APACHE IV,a mean of 59.6 was found,BAR 10.7,and MELD *** 28-day mortality rate was 9.5%,and at 90 days,it was 3.5%.The 28-day mortality prediction for APACHE IV was very good[area under the curve(AUC):0.85,P<0.001,95%CI:0.76-0.94],P<0.001,BAR(AUC:0.70,P<0.001,95%CI:0.58–0.81),and MELD(AUC:0.66,P<0.006,95%CI:0.55-0.78),P<*** 90 days,the data for APACHE IV were very good(AUC:0.80,P<0.001,95%CI:0.71–0.90)and moderate for BAR and MELD,respectively,(AUC:0.66,P<0.004,95%CI:0.55–0.77),(AUC:0.62,P<0.026,95%CI:0.51–0.72).All showed good discrimination between deaths and *** for the best value for liver transplantation,it was significant only for APACHE IV(P<0.001).CONCLUSION The APACHE IV assessment score was more accurate than BAR and MELD in predicting mortality in deceased donor liver transplant recipients.

关键词： Liver transplantation Acute physiology and chronic health evaluation IV Balance of risk Model for end-stage liver disease Mortality Intensive care unit

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Ground peach pits: alternative substrate component for seedling production

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Journal of Forestry Research 2019年第5期30卷 1779-1791页

作者： Patricia Mieth Maristela machado Araujo Maria Helena Fermino Suelen Carpenedo Aimi Daniele rodrigues Gomes Juliana de Marques Vilella Department of Forest Sciences Federal University of Santa MariaSanta MariaBrazil FEPAGRO–Agricultural Research Foundation of the State of Rio Grande do Sul Porto AlegreBrazil Federal University of Rio Grande do Sul Porto AlegreBrazil

The exploitation of agro-industrial wastes is a strategy to add value to seedling production and reduce environmental *** production chain for processed peaches generates,approximately,21,000 tons of fruit worldwide,and the pits are used mainly to generate *** objective of the study was to investigate the use of different proportions of ground peach pits(GPP)as a substrate component for seedling growth of Eucalyptus dunnii in the nursery and *** proportions of GPP mixed with either commercial substrate(CS)or with brown peat(BP)were *** physical and chemical properties of the substrates,the morphological and physiological attributes of the seedlings and their correlation with substrate properties were *** GPP increased the density,aeration space and pH and reduced total porosity,liquid retention and electric conductivity of the *** use of this component resulted in hormesis,a biphasic dose–response model in which low levels of exposure to this component stimulated biological performance of plants and high levels of exposure inhibited *** improved seedling growth in the nursery and adequate post-planting development of ***,GPP with CS is recommended at 20%of the total substrate and with BP between 15 and 30%.

关键词： Organic waste Prunus persica Eucalyptus dunnii Tree species Morphological attributes

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Influence of Vegetation on the Climate

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Journal of Environmental Protection 2023年第9期14卷 725-741页

作者： adriana Schueler Thallison rodrigues Oliveira Department of Architecture and Urbanism and Post Graduate Program in Territorial Development and Public Policies Universidade Federal Rural do Rio de Janeiro Seropédica Brasil Post Graduate Program PROFÁGUA Universidade Federal do Paraná Campo Mourão Curitiba-PR Brasil

With the growth of the cities, the environment ended up being altered and, among others, the natural cover and native vegetation were replaced by a built environment, which contributed to deep environmental disturbances. One of them, climate change, has attracted attention in recent decades, and can no longer be ignored without greatly compromising the quality of life of human beings. From the scenario in which we are inserted, it became necessary to investigate which factors significantly influence climate change in urban centers. The Federal Rural University of Rio de Janeiro was the center of this study. From this scenario, the influence of green and wooded areas on urban thermal behavior was analyzed. In this sense, three types of soil surface exposure were studied experimentally during four periods of the year.

关键词： Climate Urban Forestry Heat on Surfaces Seropédica

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Association between thrombomodulin and high mobility group box 1 in sepsis patients

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World Journal of Critical Care Medicine 2020年第4期9卷 63-73页

作者： adriana Teixeira rodrigues Julia Teixeira rodrigues Carolina Teixeira rodrigues Caroline Maria de Oliveira Volpe Fabiana Rocha-Silva Jose Augusto Nogueira-machado Luiz Ronaldo Alberti Department of Pediatrics School of MedicineFederal University of Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Graduation Program in Medicine/Biomedicine-Santa Casa Hospital-Education and Research Belo Horizonte 30150-240Minas GeraisBrazil Department of Pharmacy Federal University of Minas GeraisBelo Horizonte 31270-901Minas GeraisBrazil Department of Internal Medicine Unimed HospitalBelo Horizonte 30150-340Minas GeraisBrazil Department of Immunology Graduation Program in Medicine/Biomedicine-Santa Casa Hospital-Education and ResearchBelo Horizonte 30150-240Minas GeraisBrazil Clinical Laboratory Graduation Program in Medicine/Biomedicine-Santa Casa Hospital-Education and ResearchBelo Horizonte 30150-240Minas GeraisBrazil Department of Surgery School of MedicineFederal University of Minas GeraisBelo Horizonte 30220-000Minas GeraisBrazil

BACKGROUND High mobility group box 1(HMGB1)has been studied as a molecule associated with severe outcomes in sepsis and thrombomodulin(TM)seems to decrease HMGB1 *** To investigate the role of the thrombomodulin/high mobility group box 1(T/H)ratio in patients with sepsis and their association with their clinic,testing the hypothesis that higher ratios are associated with better *** Twenty patients diagnosed with sepsis or septic shock,according to the 2016 criteria sepsis and septic shock(Sepsis-3),were *** were followed until they left the intensive care unit or until they achieved 28 d of hospitalization(D28).The following clinical outcomes were observed:Sequential Organ Failure Assessment(SOFA)score;Need for mechanical pulmonary ventilation;Presence of septic shock;Occurrence of sepsis-induced coagulopathy;Need for renal replacement therapy(RRT);and *** The results showed that patients with SOFA scores greater than or equal to 12 points had higher serum levels of TM:76.41±29.21 pg/mL vs 37.41±22.55 pg/mL among those whose SOFA scores were less than 12 points,P=*** T/H ratio was also higher in patients whose SOFA scores were greater than or equal to 12 points,P=*** T/H ratio was,on average,three times higher in patients in need of RRT(0.38±0.14 vs 0.11±0.09),P<*** Higher serum levels of TM and,therefore,higher T/H ratio in the first 24 h after the diagnosis of sepsis were associated with more severe disease and the need for renal replacement therapy,while those with better clinical outcomes and those who were discharged before D28 showed a tendency for lower T/H ratio values.

关键词： High mobility group box 1 Sepsis Thrombomodulin Renal replacement therapy Mechanical ventilation Septic shock

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Stem cells and cell therapy: From basic sciences to clinical perspectives

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Journal of Biomedical Science and Engineering 2013年第6期6卷 683-692页

作者： Luciana machado Arnaldo rodrigues Santos Jr. Centro de Ciências Naturais e Humanas Universidade Federal do ABC Santo André SP Brazil Centro Regional Universitário de Espírito Santo do Pinhal Espírito Santo do Pinhal SP Brazil

Stem cells are seen as a possible solution for organ and tissue regeneration and for the treatment or even cure of various diseases. There are basically two types of stem cells: embryonic and adult stem cells. Embryonic stem cells are derived from embryos and exhibit an extensive multiplication and differentiation potential. On the other hand, adult stem cells, which are found in bone marrow and other tissues, have a lower multiplication and differentiation capacity but are more easily isolated and applied. In adult stem cell therapies using bone marrow or umbilical cord blood transplantation, the differentiation of a certain cell type is induced and its multiplication is stimulated, followed by the implantation of these cells into damaged tissues. Examples of the application of stem cells include the treatment of autoimmune diseases such as type 1 diabetes mellitus and heart diseases. Stem cells open up new prospects for the treatment of a series of diseases. In this respect, basic knowledge of the mechanisms of growth and differentiation of these cells is of the utmost importance to optimize therapeutic results. In this review, we discuss the mechanisms of stem cell differentiation and describe the clinical results reported in the literature, mainly by Brazilian research groups.

关键词： Stem Cells Cell Differentiation Cell Transplantation Cell Therapy

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Enhancement of seed germination in Macaranga peltata for use in tropical forest restoration

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Journal of Forestry Research 2014年第4期25卷 897-901页

作者： Cassie R.rodrigues Bernard F.rodrigues Department of Botany Goa University Goa 403206 India

We used pre-sowing treatments v/z., soaking seeds in concen- trated sulphudc acid （CSA）, gibberellic acid （GA）, combined treatment of CSA ＋ GA and mechanical scarification to overcome seed dormancy and enhance synchronous germination of Macaranga peltata seeds. We analysed percent seed germination data by logistic regression and con- finned that except in GA treatment, time and acid concentration together were crucial for enhancing germination. The combination treatment of CSA and GA resulted in higher percent germination （up to 74%） than either treatment used separately, but produced defective seedlings （26%）. Mechanical scarification of seed coat had the greatest impact in enhanc- ing germination （78%） and reducing imbibition time （6 days） against the control （0%）. Germination studies and SEM analysis confirmed that seed germination in M. peltata was inhibited by seed coat dormancy.

关键词： dormancy gibberellic acid (GA) mechanical scarification restoration seed coat

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Seismic fragility assessment of revised MRT buildings considering typical construction changes

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Frontiers of Structural and Civil Engineering 2020年第1期14卷 241-266页

作者： Rakesh DUMARU Hugo rodrigues Humberto VARUM CONSTRUCT-LESE Faculty of Engineering(FEUP)University of PortoPorto 4200-465Portugal RISCO School of Technology and ManagementPolytechnic Institute of LeiriaLeiria 2411-901Portugal

The present study investigates the vulnerability assessment of the prototype revised Mandatory Rule of Thumb(MRT)buildings initially designed and detailed for three storeys bare frame building;later modified through variable number of storeys(three,four,and five)and different arrangement of infill walls(bare frame,soft-storey,irregular infilled,and fully infilled).The application of infill walls increases the fundamental frequencies,stiffness,and maximum strength capacity,but reduces the deformation capability than the bare frame *** vulnerability was also reduced through infill walls,where the probability of exceeding partial-collapse and collapse damage reduced by 80% and 50%,***,the increased in storeys(three to five)also increases the failure probability,such that partial-collapse and collapse for fully infilled increases by almost 55% and 80%,*** obtained results and discussions concluded that the structural sections and details assigned for MRT building is not sufficient if considered as bare frame and *** increase in number of storeys causes building highly vulnerable although the infill walls were considered.

关键词： mid-rise buildings revised NBC 205:2012 masonry infill walls configurations of infill walls vulnerability assessment and inter-storey drift

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Pork Meat Quality of Preto Alentejano and Commercial Largewhite Landrace Cross

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Journal of Integrative Agriculture 2013年第11期12卷 1961-1971页

作者： Alfredo Teixeira Sandra rodrigues Veterinary and Animal Reserch Centre (CECAV) Agrarian School of Polytechnic Institute of BraganaCampus Sta Apolónia Apt.1172Bragan a 5301-855Portugal Mountain Research Centre(CIMO) Agrarian School of Polytechnic Institute of BraganaCampus Sta Apolónia Apt.1172Bragan a 5301-855Portugal

This work aimed to evaluate the physical-chemical characteristics of pork from a local breed （Portuguese black pork called Preto Alentejano） and a commercial breed. Sixteen animals were used, 4 females and 4 males from each breed. Animals had 80-100 kg of live weight. The longissimus muscle between the 5th thoracic vertebra and the 10th lumbar vertebra was used in the analysis. Samples were analysed for protein, fat, pigments, ashes, dry mater, water-holding capacity, and texture. Results of fat and pigments contents indicate signiifcant （P〈0.05 and P〈0.01） differences for all treatments. For protein, ashes, dry mater, water-holding capacity and texture, no signiifcant differences were found for sex. In the analysis of fatty acids composition, ten were detected, being the main ones C16：0, C18：0, C16：1, C18：1, C18：2. There was a predominance of monounsaturated fatty acids （MUFA）, followed by saturated （SFA） and polyunsaturated （PUFA）. Differences were signiifcant for sex （P〈0.01） and breed （P〈0.01）. Preto Alentejano breed and females presented the higher percentages of SFA （P〈0.01） and MUFA （P〈0.001） fatty acids. Differences between breeds might be due to breed production system and feed differences.

关键词： breed sex pork meat quality

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