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Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca^(2+) Signaling

Chinese Journal of Integrative Medicine 2024年第6期30卷 525-533页

作者： Lucas dos Santos Zamarioli Michele Rosana Maia Santos adolfo Garcia erustes Vanessa Marques Meccatti Thais Cristine Pereira Soraya S Smaili Maria Cristina Marcucci Carlos Rocha Oliveira Gustavo JS Pereira Claudia Bincoletto Department of Pharmacology Paulista School of MedicineFederal University of São PauloSão PauloSP04044-020Brazil Research Group on Phytocomplexes and Cell Signaling School of Health SciencesAnhembi Morumbi UniversitySão PauloSP03164-000Brazil Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State University(ICT-UNESP)São PauloSP12231-280Brazil

Objective To evaluate the chemical composition and effects of Artemisia vulgaris(AV)hydroalcoholic extract(HEAV)on breast cancer cells(MCF-7 and SKBR-3),chronic myeloid leukemia(K562)and NIH/3T3 *** Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass(HPLC)*** and cell death studies were performed using trypan blue and Annexin/FITC-7AAD,***-1(Fer-1)and necrostatin-1(Nec-1)were used to assess the mode of HEAV-induced cell death and acetoxymethylester(BAPTA-AM)was used to verify the involvement of cytosolic calcium in this *** calcium measurements were made using *** HEAV decreased the viability of MCF-7,SKBR-3 and K562 cells(P<0.05).The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts(P<0.05).Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD(P<0.05).The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone(P<0.01).HEAV-induced Ca^(2+)release mainly from lysosomes in K562 cells(P<0.01).Furthermore,BAPTA-AM,an intracellular Ca^(2+)chelator,decreased the number of non-viable cells treated with HEAV(P<0.05).Conclusions HEAV is cytotoxic and activates several modalities of cell death,which are partially dependent on lysosomal release of Ca^(2+).These effects may be related to artemisinin and caffeoylquinic acids,the main compounds identified in HEAV.

关键词： Artemisia vulgaris necroptosis ferroptosis late apoptosis Ca^(2+)signaling

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Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

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Journal of Integrative Medicine 2019年第2期17卷 132-140页

作者： Carlos R.Oliveira Daniel G.Spindola Daniel M.Garcia adolfo erustes Alexandre Bechara Caroline Palmeira-dos-Santos Soraya S.Smaili Gustavo J.S.Pereira André Hinsberger Ezequiel P.Viriato Maria Cristina Marcucci Alexandra C.H.F.Sawaya Samantha L.Tomaz Elaine G.Rodrigues Claudia Bincoletto Departamento de Farmacologia Escola Paulista de Medicina (EPM) Universidade Federal de Sao Paulo (UNIFESP) Grupo de Fitocomplexos e Sinalizacao Celular Escola de Ciências da Saúde Universidade Anhembi Morumbi Faculdade de Ciências Farmacêuticas e Bioquímicas Oswaldo Cruz Laboratório de Produtos Naturais e Quimiometria Programa de pós-graduacao em Farmácia e Biotecnologia Universidade Anhanguera de Sao Paulo Faculdade de Ciências Farmacêuticas Universidade Estadual de Campinas (UNICAMP) Unidade de Oncologia Experimental EPM Universidade Federal de Sao Paulo (UNIFESP)

Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its *** study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root(CEAA) on breast ***: The cytotoxicity of CEAA against breast adenocarcinoma cells(4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide(PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4 NW. Immunoblotting was used to determine the effect of CEAA on anti-and pro-apoptotic proteins(Bcl-2 and Bax, respectively).The 4 T1 cell-challenged mice were used for in vivo ***: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study(73 mg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4 T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA(500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated ***: Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy.

关键词： Angelica archangelica Umbelliferae Apoptosis Cell death Breast cancer Angelicin

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A novel approach to the dynamic response analysis of Euler-Bernoulli beams resting on a Winkler soil model and subjected to impact loads

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Earthquake Engineering and Engineering Vibration 2024年第2期23卷 389-401页

作者： adolfo Foriero Filippo Santucci de Magistris Giovanni Fabbrocino Département de génie civil et de génie des eaux Pavillon Adrien-PouliotUniversitéLavalQuébecCanada Dipartimento di Bioscienze e Territorio Universitàdegli Studi del MoliseCampobassoItaly Construction Technologies Institute National Research CouncilITC-CNRL’AquilaItaly

This work presents a novel approach to the dynamic response analysis of a Euler-Bernoulli beam resting on a Winkler soil model and subjected to an impact *** approach considers that damping has much less importance in controlling the maximum response to impulsive loadings because the maximum response is reached in a very short time,before the damping forces can dissipate a significant portion of the energy input into the *** development of two sine series solutions,relating to different types of impulsive loadings,one involving a single concentrated force and the other a distributed line load,are *** study revealed that when a simply supported Euler-Bernoulli beam,resting on a Winkler soil model,is subject to an impact load,the resulting vertical displacements,bending moments and shear forces produced along the span of the beam are considerably *** particular,the quantification of this effect is best observed,relative to the corresponding static solution,via an amplification *** computed impact amplification factors,for the sub-grade moduli used in this study,were in magnitude greater than 2,thus confirming the multiple-degree-of-freedom nature of the problem.

关键词： beam-Winkler-soil model sub-grade moduli impact load impact distributed line load dynamic solution impact amplification factor

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HVSR analysis of pumice sands for sediment depth characterization:A case study for Guadalajara,Mexico

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Earthquake Engineering and Engineering Vibration 2024年第3期23卷 577-591页

作者： Hafid Salgado M. Alejandro Ramírez-Gaytan adolfo Preciado Christian R.Escudero Departamento de Ciencias Exactas Centro Universitario de la CostaUniversidad de GuadalajaraCP.48280 Puerto VallartaJaliscoMéxico Departamento de Ciencias Computacionales Centro Universitario de Ciencias Exactas e IngenieríaUniversidad de GuadalajaraCP.44430 GuadalajaraJaliscoMéxico Departamento del Hábitat y Desarrollo Urbano Instituto Tecnológico y de Estudios Superiores de Occidente(ITESO)CP.45604 TlaquepaqueMéxico

The horizontal to vertical spectral ratio(HVSR)methodology is used here to characterize pumice soils and to image the three-dimensional surface geometry of Guadalajara,*** to other Latin American cities,Guadalajara is exposed to high seismic risk,with the particularity of being the largest urban settlement in Latin America built on pumice *** has not yet been tested to characterize subsoil depths in pumice *** to the questionable use of traditional geotechnical tests for the analysis of pumice soils,HVSR provides an alternative for its characterization without altering its fragile and porous *** this work,resonance frequency(F0)and peak amplitude(A0)are used to constrain the depth of the major impedance contrast that represents the interface between bedrock and pumice *** were compared with borehole depths and other available geotechnical and geophysical data and show good *** of the profiles estimated on the riverbanks that cross the city,reveals different subsoil thickness that could have an impact on different site responses on riverine areas to an eventual *** and academic efforts are combined in this work to characterize depth sediments,an important parameter that impacts the regulations for construction in the city.

关键词： subsoil of Guadalajara shallow soil thickness bedrock depth pumice soil characterization

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Endoscopic treatment of scarred polyps with a non-thermal device(Endorotor):A review of the literature

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World Journal of Gastroenterology 2024年第12期30卷 1706-1713页

作者： Mariam Zaghloul Hameed Rehman Stefano Sansone Konstantinos Argyriou adolfo Parra-Blanco Department of Hepatology Gastroenterology and Infectious DiseasesKafrelsheikh UniversitKafr-Elshikh 33516Egypt Department of Gastroenterology Service Nottingham City Hospital CampusNottingham University Hospitals NHS TrustNottingham NG51PBUnited Kingdom Department of Gastroenterology University Hospital of LarissaLarissa 41334Greece NIHR Nottingham Biomedical Research Centre Nottingham University Hospitals NHS Trust and University of NottinghamNottingham NG72UHUnited Kingdom

Endoscopic resection(ER)of colorectal polyps has become a daily practice in most endoscopic units providing a colorectal cancer screening program and requires the availability of local experts and high-end endoscopic *** procedures have evolved over the past few years from endoscopic mucosal resection(EMR)to more advanced techniques,such as endoscopic submucosal dissection and endo-scopic full-thickness *** resection and disease eradication are the ultimate goals of ER-based techniques,and novel devices have been developed to achieve these *** EndoRotor®Endoscopic Powered Resection System(Interscope Medical,Inc.,Northbridge,Massachusetts,United States)is one such *** EndoRotor is a powered resection tool for the removal of alimentary tract mucosa,including post-EMR persistent lesions with scarring,and has both CE Mark and FDA *** review covers available published evidence documenting the usefulness of EndoRotor for the management of recurrent colorectal polyps.

关键词： EndoRotor Scarred polyps Recurrent polyps Colorectal cancer Colorectal polyps

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Changes in call properties of Boana pulchella (Anura, Hylidae) in response to different noise conditions

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Current Zoology 2024年第4期70卷 548-556页

作者： Pablo Grenat Maria Ferrero Mariana Baraquet Favio Pollo Manuel Otero Zulma Salinas Nancy Salas adolfo Martino Ecologia Departamento de Ciencias NaturalesFacultad de Ciencias ExactasFisico-Quimicas y NaturalesUniversidad Nacional de Rio CuartoRutaNacional N°36-km601X5804BYARioCuartoArgentina lnstituto de Ciencias de la Tierra Biodiversidad y Ambiente(ICBIA)UNRC-CONICETRuta Nacional N°36-km 601X5804BYARio CuartoArgentina Cátedra de Botanica General Departamento de Recursos Naturales y AmbienteFacultad de AgronomiaUniversidad de Buenos AiresAv.San Martin 4453Buenos AiresArgentina

The increasing expansion of urban areas leads to the emergence of new noisy environments that can affect animal *** play a crucial role in the mating displays of anurans,and the negative impact of anthropogenic noise-induced auditory masking has been reported in several *** investigated the acoustic variation in 96 males(n=971 calls)of the treefrog Boana pulchella across acoustically undis-turbed sites and different noise conditions,associated with urban areas(URBAN)and roads(ROAD),in Central *** analyzed the effect of anthropogenic noise conditions on 6 temporal(call duration[CD],intercall interval,first and second note duration,internote interval,and call rate)and 3 spectral(dominant frequency of first and second note,frequency difference between them)call *** effects of temperature and size on acoustical variables were *** observed differences in all call attributes among the noise conditions,except for intercall *** exposed to URBAN and ROAD exhibited significant changes in CD,dominant frequency of the second note,and fre-quency difference between *** males had longest internote interval,while ROAD individuals displayed increased first and second note duration and call ***,ROAD males exhibited immediate changes in call rate in direct response to passing heavy *** study emphasizes the impact of anthropogenic noise on the acoustic characteristics of *** *** how animals adapt to noisy environments is crucial in mitigating the adverse effects of urbanization on their communication *** investigations should explore whether the observed call adjustments are effective in avoiding or mitigating the negative consequences of anthropogenic noise on reproductive success.

关键词： acoustic communication advertisement call auditory masking noise pollution road traffic noise.

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Highly divergent sympatric lineages of Leptotila verreauxi(Aves:Columbidae)suggest a secondary contact area in the Isthmus of Tehuantepec,Mexico

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Avian Research 2024年第1期15卷 12-21页

作者： Orlando J.Espinosa-Chávez adolfo G.Navarro-Sigüenza Hernando Rodríguez-Correa Luis A.Sánchez-González Museo de Zoología“Alfonso L.Herrera” Depto.de Biología EvolutivaFacultad de CienciasUniversidad Nacional Autonoma de MexicoApdo.Postal 70-399CP 04510Ciudad de MexicoMexico Posgrado en Ciencias Biologicas Unidad de PosgradoEdificio D1◦PisoCircuito de PosgradosCiudad UniversitariaUniversidad Nacional Autonoma de MexicoCoyoacanCP 04510CDMXMexico Escuela Nacional de Estudios Superiores Unidad Morelia Universidad Nacional Autonoma de Mexico.Antigua Carretera a Patzcuaro No.8701 Col.Ex Hacienda de San Jose de la HuertaC.P.58190MoreliaMichoacanMexico

Due to a complex geological and biotic history,the Isthmus of Tehuantepec(IT),has been long recognized as a driver for the evolutionary divergence of numerous lowland and highland *** distributed in the lowlands of the American continent,the White-Tipped Dove(Leptotila verreauxi)is a polytypic species with 13 recognized *** of these have been recorded in Mexico,and the distribution of three abuts at the IT,suggesting a contact *** estimate phylogenetic patterns,divergence times and genetic differentiation,we examined two mt DNA(ND2 and COI)and one n DNA(β-fibint 7)*** also used correlative ecological niche models(ENM)to assess whether ecological differences across the IT may have acted as a biogeographical *** estimated paleodistributions during the Middle Holocene,Last Glacial Maximum and Last Interglacial,to evaluate the influence of climate changes on the distribution and demographic *** results showed genetically distinct lineages that diverged approximately 2.5 million years *** and ecological factors may have played a dual role in promoting differentiation,but also in the formation of a secondary contact zone in the southern *** ecological niche comparisons indicated that the ecological niche of sympatric lineages at the IT are not identical,suggesting niches divergence;in addition,environmental niche models across the region indicated no abrupt biogeographic barriers,but the presence of regions with low *** results suggest that genetic differentiation originated by a vicariant event probably related to environmental factors,favored the evolution of different ecological ***,the absence of a biogeographic barrier but the presence of less suitable areas in the contact regions,suggest that secondary contact zones may be also maintained by climatic factors for the eastern group,but also by biotic interactions for the western group.

关键词： Biogeographical boundary Humid and dry forests Isthmus of Tehuantepec Phylogeography Secondary contact

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Impacts of brood parasitism by shiny cowbird Molothrus bonariensis on the breeding success of a small host, the black-backed water tyrant Fluvicola albiventer

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Current Zoology 2024年第4期70卷 539-547页

作者： Lorena Vanesa Sovrano Evelina Jesica Leon Rodrigo Ezequiel Lorenzón Pamela Fernanda Olguin adolfo Héctor Beltzer Alejandro Raúl Giraudo Laboratorio de Biodiversidad y Conservacion de Tetrapodos Instituto Nacional de Limnologia(INALICONICET-UNL)Paraje El Pozo s/nSanta Fe(3000)Argentina Departamento de Biologia Universidad Autonoma de Entre RiosKm 10.5 RP 11Oro Verde(3100)Entre RiosArgentina Departamento de Biologia Universidad Nacional del LitoralFacultad de Humanidades y CienciasParaje Ei Pozo s/nSanta Fe(3000)Argentina

The shiny cowbird Molothrus bonariensis parasitizes many species with different life-history traits and has a detrimental effect on the survival of the progeny of the *** response,hosts have developed numerous antiparasitic ***,we examined the effects of brood parasitism by shiny cowbird on the clutch and brood sizes(83 nests)in a small host,the black-backed water tyrant Fluvicola *** also studied whether the death of parasite nestlings was related to the care of the foster parents and whether the host had any antiparasitic defense against the shiny *** results indicate that brood parasitism significantly decreased the host hatching and fledging *** majority of nest failures(57%)were caused by brood *** cowbird parasitism occurred in 52%of nests and the intensity of parasitism was 1.23±0.53 eggs per parasitized *** the total host eggs,54%were *** the incubation stage,20 nests(47%)were abandoned because of egg punctures by shiny cowbirds *** two parasitic fledglings were recorded,while the remaining nestlings either died from starvation(n=12)or predation(n=3).Foster parents abandoned parasitic nestlings between 5 and 10 days *** findings demonstrate that the shiny cowbird has very low rates of fledging success when parasitizing black-backed water ***,parasitism had a high reproductive cost in the black-backed water tyrant because a very low proportion(7%)of the parasitized nests(n=43)were successful.

关键词： brood parasitism host defenses nest desertion shiny cowbird small host.

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Vanadium mineralization at Los Chihuidos sediment-hosted Cu-V deposit,Neuquén Basin,Argentina:An approach to vanadium ore forming processes

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Geoscience Frontiers 2024年第1期15卷 225-246页

作者： Ana L.Rainoldi Daniel Beaufort Marta B.Franchini adolfo Giusiano Sabine Petit Patricia Patrier M.Josefina Pons Dpto.de Geología Universidad Nacional del Sur(UNS)8000 Bahía BlancaArgentina Consejo Nacional de Investigaciones Científicas y Técnicas Centro Patagónico de Estudios MetalogenéticosArgentina Universitéde Poitiers IC2MPCNRS-UMR 7285HydrasaF-86022 Poitiers CedexFrance Universidad Nacional del Comahue Facultad de IngenieríaDepartamento de Geología y Petróleo8300 NeuquénArgentina Consultant Geologist 8300 NeuquénArgentina Universidad Nacional de Río Negro Instituto de Investigación en Paleobiología y GeologíaRío NegroArgentina

Vanadium mineralization at Los Chihuidos deposit of the Neuquén Basin is linked to the development of a redox front system related to the inflow of hydrocarbons into the red sandstone of the Huincul *** of hydrocarbons with oxidized red beds and connate water generated redox reactions where hematite was dissolved due to iron reduction resulting in the discoloration of the red *** the contact between oxidized red sandstone and reduced white sandstone,precipitation of specific mineral phases resulted in the V ore with minor amounts of *** the implementation of the redox interface,abundant V-montmorillonite and V-hematite precipitated at the more oxidizing conditions and Cu-V-corrensite-type at the more reducing conditions of the redox *** the redox front advanced with fluids constantly migrating into the reservoir,more reducing conditions were stablished,promoting chloritization and minor illitization with V-Cu incorporation and continuous upgrading of the *** ore mineralogy consists of clay minerals including V-bearing montmorillonite,Cu-V-corrensite-type,V-di-trioctahedral chlorite and Cu-tri-trioctahedral chlorite with minor V-illite-smectite mixed-layer minerals and associated secondary *** over illitization was favored due to high amounts of Fe and Mg in detrital clasts and in connate fluids and by low K availability related to low amounts of detrital *** spatial transition of V and/or Cu bearing clay minerals observed through the mineralized redox front at Los Chihuidos deposit(kaolinite→smectite→illite/smectite→corrensite-type→di-trioctahedral-chlorite→tri-trioctahedral-chlorite)and the related variation of V-Cu concentrations in bulk rock are indicative of increasing pH and decreasing Eh of resident solutions from red to white sandstones during the hypogene mineralization *** influx of Cu-rich oxidized basinal brines precipitated main copper ore with Cu-sulfides in the white sandst

关键词： Clay minerals V-chlorite Redox front Bleaching Hydrocarbons

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ITPK1 is an InsP_(6)/ADP phosphotransferase that controls phosphate signaling in Arabidopsis

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Molecular Plant 2024年第7期17卷 1151-1157页

作者： Esther Riemer Danye Qiu Debabrata Laha Robert K.Harmel Philipp Gaugler Verena Gaugler Michael Frei Mohammad-Reza Hajirezaei Nargis Parvin Laha Lukas Krusenbaum Robin Schneider adolfo Saiardi Dorothea Fiedler Henning J.Jessen Gabriel Schaaf Ricardo F.H.Giehi 不详

(Molecular Plant 14,1864-1880;November 12021)We identified a mistake made when calculating the absolute concentrations of inositol(pyro)phosphates derived from CE-ESl-MS measurements reported in the originally published version of our *** to a calculation error when determining concentrations per fresh biomass,the absolute values shown in the manuscript are approximately 30-fold lower than they should *** mistake affects Figures 2D,3C,4C,and 7D and Figures S4,S11B,S13,and *** all conclusions made in the article referred to relative differences between genotypes and treatments,they remained unaffected by these mistakes.

关键词： ipt Arabidopsis absolute

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