检索结果-南通市图书馆

Antimethicilin resistance agents from marine actinomycetes from soil sediments of Lagos Lagoon

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Coastal Life Medicine 2015年第3期 193-198页

作者： Davies Olabisi Flora adeleye isaac adeyemi Wang Peng George Department of Microbiology University of LagosAkokaLagos StateNigeria Department of Chemistry Georgia State UniversityAtlantaGeorgiaUSA

Objective:To evaluate the isolation of actinomycetes strains with potential for producing antimicrobials with high methicilin resistance ***:The soil samples were collected from four different locations of Lagos *** Actinomycetes were isolated from the samples by serial dilution using spread plate *** were selected based on their cultural characteristics as well as their Gram reaction and phenotypically and molecularly characterized Streptomyces *** were inoculated in starch casein and Kuster’s broth media and secondary metabolites were screened for antimicrobial activity against the following microorganisms:methicillin resistant Staphylococcus aureus,Staphylococcus aureus ATCC 29213,Escherichia coli ATCC 29522,Pseudomonas aeruginosa ATCC 27853,Candida albicans,Enterococcus faecalis ATCC ***-negative staphylococci isolated from HIV patients were also used(Staphylococcus warneri,Staphylococcus xylosus and Staphylococcus epidermidis).The antimicrobial metabolites of the isolates were identified using gas chromatography-mass ***:Extracts from isolates ULS12 and ULS13 showed antimicrobial activity against methicillin resistant Staphylococcus aureus while ULK3 inhibited Candida albicans *** gas chromatography-mass spectrometer data analysis showed the antibiotic profile of these ***:The isolates ULS12 and ULS13 were found to display the highest antimicrobial activity against the test organisms and could be a potential source of new antibiotics.

关键词： Marine actinomycetes Streptomyces Antimethicillin resistance Molecular identification Morphological characteristics GC-MS

Himalomycin A and cycloheximide-producing marine actinomycete from Lagos Lagoon soil sediment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Coastal Life Medicine 2015年第5期 361-365页

Objective:To isolate and screen Actinomycetes from Lagos Lagoon soil sediments for antibiotic ***:Soil samples were collected from four different locations of Lagos Lagoon and were dried for 2 *** were isolated by serial dilution using spread plate method on starch casein and Kuster’s agar supplemented with 80μg/mL cycloheximide to prevent fungal *** plates were incubated at 28°C for 1-2 *** were selected based on their cultural characteristics as well as their Gram’s reaction and subcultured on same media for isolation and incubated at 28°C for 3 *** cultures were maintained on nutrient agar slants at 4°***,they were inoculated into starch casein and Kuster’s broth media and incubated at 28°C for 8 *** resulting crude extracts were screened for antimicrobial activity against the following microorganisms:methicillin resistant Staphylococcus aureus,Staphylococcus aureus ATCC 29213,Escherichia coli ATCC 29522,Pseudomonas aeruginosa ATCC 27853,Candida albicans and Enterococcus faecalis ATCC ***-negative staphylococci isolated from HIV patients were also used(Staphylococcus warneri,Staphylococcus xylosus and Staphylococcus epidermidis).Extraction of secondary metabolites was carried out and analysed using gas chromatography-mass ***:All the isolates displayed varying antimicrobial activity against at least one of the test *** A was identified in the extract from isolate *** gas chromatography-mass spectrometer data analysis showed the antibiotic profile of these ***:The isolate ULS7 was found to display the highest antimicrobial activity against the test organisms.

关键词： Marine Actinomycetes Streptomyces Himalomycin A Cycloheximide Molecular identification Morphological characteristics Gaschromatography-mass spectrometer

Anticancer potential of metabolic compounds from marine actinomycetes isolated from Lagos Lagoon sediment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2019年第3期9卷 201-208页

作者： Olabisi Flora Davies-Bolorunduro isaac adeyemi adeleye Moshood Olushola Akinleye Peng George Wang Department of Microbiology University of Lagos Akoka Lagos Nigeria Department of Pharmaceutical Chemistry University of Lagos Idi-Araba Lagos Nigeria Department of Chemistry Georgia State University Atlanta GA United States

Thirty-two actinomycetes strains were isolated from sediment samples from 12 different sites at Lagos Lagoon and identified using standard physiological and biochemical procedures as well as 16S rDNA gene sequence analysis. Secondary metabolites were extracted from the strains and their anticancer activity on the K562 (Human acute myelocytic leukemia), HeLa (cervical carcinoma), AGS (Human gastric), MCF- 7 (breast adenocarcinoma) and HL-60 (Human acute promyelocytic leukemia) cell lines was determined. The metabolic extracts exhibited cytotoxicity with IC50 values ranging from 0.030 mg/mL to 4.4 mg/mL. The Streptomyces bingchenggensis ULS14 extract was cytotoxic against all the cell lines tested. The bioactivity-guided extraction and purification of the metabolic extracts from this strain yielded two purified anticancer compounds: ULDF4 and ULDF5. The structures of the extracted compounds were determined using spectroscopic analyses, including electrospray ionization mass spectrophotometer and nuclear magnetic resonance (1 Dimensional and 2 Dimensional), and were shown to be structurally similar to staurosporine and kigamicin. The IC50 of ULDF4 and ULDF5 against the HeLa cell line was 0.034 mg/mL and 0.075 mg/mL, respectively. This study is the first to reveal the anticancer potential of actinomycetes from Lagos Lagoon, which could be exploited for therapeutic purposes.

关键词： Streptomyces bingchenggensis ULS14 Metabolic extracts Cytotoxicity Spectroscopy Kigamicin Staurosporine

Heavy Metal Profile of Oreochromis niloticus Harvested from E-Waste Polluted Vials and Associated Fungi

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances in Microbiology 2016年第8期6卷 555-565页

作者： adeyemi isaac Sanusi Department of Microbiology Federal University of Technology Akure Nigeria

This study was designed to investigate the possible variation in bioaccumulation of heavy metals (lead, cadmium, zinc, cobalt, chromium, nickel, and manganese) in the tissue of harvested Oreochromis niloticus (tilapia) and the associated fungi from vials treated with soil from e-waste dumpsite and soil without e-waste. E-waste is electronic waste which contains valuable metals as well as potential environmental contaminants. The heavy metals in soil and fish samples were determined using flame atomic absorption spectrophotometer (AAS) after homogeneity and digestion of samples. The associated fungi were identified using standard microbiological methods. The genera of the associated fungi were: Penicillium, Candida, Articulospora, Aspergillus, Aspergillus, Aspergillus, Rhizopus, Mucor, Zoopage, Varicosporium and Rhodoturula. The quantity of fungi isolated from each pollution treatments showed that the number of fungi were inversely proportional to the quantity of e-waste soil pollution and directly proportional to the quantity of soil without e-waste pollution. The occurrence of the fungi species revealed that the untreated (control) vial had the highest occurrence (191) while the lowest occurrence (103) occurred in the vial polluted with the highest quantity of soil from e-waste dumpsite. The pH and the biochemical oxygen demand (BOD) of the vials were significantly affected by the pollutions. Variations were also observed in the bioaccumulation of the heavy metals by Oreochromis niloticus. Cadmium (0.01 mg/kg) and nickel (0.02 - 0.08 mg/kg) were the least recorded metal in the tissue of the fish while cobalt was not detected after the five weeks period. The sequence of the heavy metals concentrations in Oreochromis niloticus tissue samples was Zn > Mn > Pb > Cr > Ni > Cd > Co. The accumulation of the metals in the fish tissue were, however, lower than the international maximum guidelines, except for manganese (0.29 - 3.13 mg/kg) that exceeded the 0.01 - 0.05 mg/kg

关键词： Bioaccumulation Fungi Heavy Metals Vial E-Waste Soil Oreochromis niloticus

Hepatitis B Virus (HBV) Infection in Pregnancy: Knowledge and Practice of Care Providers in Nigeria

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Obstetrics and Gynecology 2014年第10期4卷 621-627页

作者： Adewale S. adeyemi Adeola F. Afolabi adeleye A. Adeomi Department of Community Medicine Ladoke Akintola University of Technology Teaching Hospital Ogbomoso Nigeria Department of Obstetrics and Gynaecology College of Health Sciences Ladoke Akintola University of Technology Ogbomoso Nigeria

Purpose: To determine the practice of obstetric care providers regarding routine screening for hepatitis B virus infection, and to assess their knowledge of the management of the positive pregnant women. Methods: A cross sectional descriptive study of obstetric care providers in southwestern Nigeria. Mean and standard deviation was used to summarize continuous variables while frequency and percentage was used for categorical variables. Categorical variables were compared with chi-square test and Fisher’s exact tests as appropriate;while continuous variables were compared using the t-test. The level of statistical significance was set at P ≤ 0.05. Scoring of the outcome variables for knowledge was done, and the score categorized into good and poor knowledge, depending on if the respondent scored above or below the mean score. Results: Three hundred and eighty-one (67.4%) of the care givers routinely screen pregnant women for the serum marker (HBSAg) of HBV infection. Two hundred and seventy-four (48.5%) of the respondents had good knowledge score of the management of HBV infection in pregnancy. Routine screening for HBV infection was significantly associated with age (p = 0.002), years of practice (p < 0.001), specialty (p = 0.001) and professional cadre (p < 0.001), while knowledge was significantly associated with age (p = 0.012), years of practice (p = 0.003), specialty (p = 0.047) and types of practice (p = 0.014). Conclusion: Screening for HBV infection in pregnant women is not universal in southwestern Nigeria, and the care providers have poor knowledge of the management of the positive mothers.

关键词： HBV Infection Obstetric Care Providers Routine Screening Management Nigeria