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Postnatal ex vivo rat model for longitudinal bone growth investigations

Animal Models and Experimental Medicine 2019年第1期2卷 34-43页

作者： adamu abdul abubakar Sahar Mohammed Ibrahim Ahmed Khalaf Ali Kareem Obayes Handool Mohammad Shuaib Khan Mohamed Noordin Mustapha Tengku Azmi Ibrahim Ubedullah Kaka Loqman Mohamad Yusof Department of Companion Animal Medicine and Surgery Universiti Putra Malaysia Department of Veterinary Surgery and Radiology Usmanu Danfodiyo University Department of Surgery and Theriogenology College of Veterinary Medicine University of Mosul Faculty of Veterinary and Animal Science Gomal University Department of Veterinary Pathology and Microbiology Universiti Putra Malaysia Department of Pre-Clinical Veterinary Sciences Universiti Putra Malaysia Laboratory of Sustainable Animal Production and Biodiversity Institute of Tropical Agriculture and Food SecurityUniversiti Putra Malaysia

Background: Chondrocytes in the growth plate(GP) undergo increases in volume during different cascades of cell differentiation during longitudinal bone growth. The volume increase is reported to be the most significant variable in understanding the mechanism of long bone ***: Forty-five postnatal Sprague-Dawley rat pups, 7-15 days old were divided into nine age groups(P7-P15). Five pups were allocated to each group. The rats were sacrificed and tibia and metatarsal bones were harvested. Bone lengths were measured after 0, 24, 48, and 72 hours of ex vivo incubation. Histology of bones was carried out, and GP lengths and chondrocyte densities were ***: There were significant differences in bone length among the age groups after 0 and 72 hours of incubation. Histological sectioning was possible in metatarsal bone from all age groups, and in tibia from 7-to 13-day-old rats. No significant differences in tibia and metatarsal GP lengths were seen among different age groups at 0 and 72 hours of incubation. Significant differences in chondrocyte densities along the epiphyseal GP of the bones between 0 and 72 hours of incubation were observed in most of the age ***: Ex vivo growth of tibia and metatarsal bones of rats aged 7-15 days old is possible, with percentage growth rates of 23.87 ± 0.80% and 40.38 ± 0.95%measured in tibia and metatarsal bone, respectively. Histological sectioning of bones was carried out without the need for decalcification in P7-P13 tibia and P7-P15 metatarsal bone. Increases in chondrocyte density along the GP influence overall bone elongation.

关键词： bone growth model chondrocytes endochondral ossification growth plate Sprague-Dawley rat

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Fusarium wilt of banana:Current update and sustainable disease control using classical and essential oils approaches

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Horticultural Plant Journal 2023年第1期9卷 1-28页

作者： abubakar abubakar Ismaila Khairulmazmi Ahmad Yasmeen Siddique Muhammad Aswad abdul Wahab abdulaziz Bashir Kutawa adamu abdullahi Syazwan Afif Mohd Zobir Arifin Abdu Siti Nor Akmar abdullah Department of Plant Protection Faculty of AgricultureUniversiti Putra Malaysia(UPM)SerdangSelangor 43400Malaysia Department of Integrated Science School of Secondary Education(Science)Federal College of Education(Technical)BichiP.M.B 3473Kano StateNigeria Department of Agriculture Technology Faculty of AgricultureUniversiti Putra Malaysia(UPM)SerdangSelangor 43400Malaysia Department of Plant Science and Biotechnology Faculty of Life ScienceFederal University Dutsin-MaP.M.B 5001Dutsin-MaKatsina StateNigeria Laboratory of Sustainable Agronomy and Crop Protection Institute of Plantation Studies(IKP)Universiti Putra Malaysia(UPM)SerdangSelangor 43400Malaysia Department of Forest Management and Biodiversity Faculty of Forestry and EnvironmentUniversiti Putra Malaysia(UPM)SerdangSelangor 43400Malaysia Department of Biological Sciences Faculty of ScienceSokoto State University8 Birnin Kebbi RoadSokoto StateNigeria

Fusarium species were reported to produce *** are superficial societies of microbes bounded and endangered by being situated or taking place outside a cell or *** most destructive fungal diseases caused by phytopathogens are as a result of biofilms *** wilt of banana(Panama disease)is caused by a soil-borne pathogen called Fusarium oxysporum *** oxysporum occurs in a form of a species complex(FOSC)which encompasses a crowd of *** genetic factor transfer may donate to the observed assortment in pathogenic strains,while sexual reproduction is unknown in the *** wilt is a notorious disease on several crops *** loss caused by this pathogen is huge,and significant to destroy crop yields annually,thereby affecting the producer countries in various continents of the *** disease is also resistant to various synthetic chemical ***,excessive use of synthetic fungicides during disease control could be lethal to humans,animals,and *** calls for alternative eco-friendly management of this disease by targeting the biofilms formation and finally suppressing this devastating *** this review,we,therefore,described the damage caused by Fusarium wilt disease,the concept of filamentous fungal biofilms,classical control strategies,sustainable disease control strategies using essential oils,and prevention and control of vegetables Fusarium wilt diseases.

关键词： Banana Fusarium oxysporum Fusarium wilt disease Antibiofilm Antifungal Essential oils

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Geotechnical forensic investigation of a slope failure on silty clay soil--A case study

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Frontiers of Structural and Civil Engineering 2020年第2期14卷 501-517页

作者： Mohammad abubakar NAVEED Zulfiqar ALI abdul QADIR Umar Naveed LATIF Saad HAMID Umar SARWAR Military College of Engineering National University of Sciences and TechnologyRisalpur 24080Pakistan School of Civil Environmental and Mining EngineeringThe University of AdelaideAdelaibe 5096Australia

Qila Bala Hisar is one of the noteworthy places of Peshawar,Khyber *** fort was constructed on a flled ground during the 18th century and it was renovated several times by the occupants ever ***,due to an earthquake of magnitude 7.3,the upper part of the south-western wall of the fort *** collapse of the wall was attributed to the failure of the retained *** research was undertaken to characterize the slope material,study causal factors of failure and evaluate remedial *** investigation involved conventional field and laboratory testing and geophysical investigation using electrical resistivity technique to evaluate the nature of ***,X-ray Diffraction and Scanning Electron Microscopy was used to study the slope material at a molecular level to evaluate the existence of swelling *** analysis has shown that excessive seepage of water caused by the poor maintenance of runoff and sewage drains is the causal factor triggered by the seismic event.A remedial strategy involving soil nails,micro piles and improvement of the surface drainage is recommended.

关键词： forensic geotechnical investigation slope failure slope analysis finite element method numerical analysis seismic loading Qila Bala Hisar

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Antiacanthamoebic properties of natural and marketed honey in Pakistan

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Asian Pacific Journal of Tropical Biomedicine 2016年第11期6卷 967-972页

作者： Farzana abubakar Yousuf Malik Hassan Mehmood abdul Malik Ruqaiyyah Siddiqui Naveed Ahmed Khan Department of Biological and Biomedical Sciences Aga Khan University Department of Biological Sciences Faculty of Science and Technology Sunway University

Objective: To determine antiacanthamoebic activity of natural and marketed honey ***: Natural honey samples were collected directly from the bee hive and marketed honey samples were purchased from the local market in Karachi, Pakistan. Both honey samples were tested for their flavonoid content(quercetin equivalent per gram of the extract) and phenolic content(gallic acid equivalent per gram). Furthermore, their antioxidant activity was determined by measuring 2,2-diphenyl-1-picrylhydrazyl. Using amoebistatic and amoebicidal assays, the effects of honey samples were tested against growth and viability of Acanthamoeba ***: Natural honey exhibited potent amoebistatic and amoebicidal effects, in a concentration-dependent manner. Honey-treated Acanthamoeba castellanii showed loss of acanthopodia, following which amoebae detached, rounded up, reduced in size,decreased in cytoplasmic mass and they were observed floating in the culture ***, honey-treated amoebae did not revive when inoculated in fresh growth medium, however, glycerol-treated amoebae exhibited viable trophozoite and active growth. In contrast, marketed honey samples varied in their efficacy against Acanthamoeba castellanii. The proportion of flavonoid, as determined by quercetin measurements and the proportion of phenolic, as determined by gallic acid measurements was higher in natural honey compared with marketed honey. Similarly, the antioxidant activity, as determined by 2,2-diphenyl-1-picrylhydrazyl scavenging activity was higher in natural honey vs. marketed ***: This study shows that natural honey has antiacanthamoebic properties and possesses higher flavonoid, phenolic and antioxidant properties compared with the marketed honey. These findings are of concern to the public, health officials, and to the manufacturers regarding production of honey for medical applications.

关键词： Honey Acanthamoeba Amoebicidal Amoebistatic

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Energy-efficient Virtual Machine Allocation Technique Using Flower Pollination Algorithm in Cloud Datacenter:A Panacea to Green Computing

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Journal of Bionic Engineering 2019年第2期16卷 354-366页

作者： Mohammed Joda Usman abdul Samad Ismail Hassan Chizari Gaddafi abdul-Salaam Ali Muhammad Usman abdulsalam Yau Gital Omprakash Kaiwartya Ahmed Aliyu Department of Maths Bauchi State University GadauBauchi 81007Nigeria Department of Computer Science Universiti Teknology MalaysiaSkudai Johor 81310Malaysia School of Computing and Technology Park CampusUniversity of Gloucestershire Cheltenham GL50 4SHUK Department of Computer Science Kwame Nkrumah University of Science and TechnologyKumasi 00233Ghana Department of Maths and Computer Federal College of Education Technical GombeGombe 072158Nigeria Department of Maths Abubakar Tafawa Balewa University BauchiBauchi 81027Nigeria Department of Computer and Information Technology Northumbria UniversityNewcastle NE1SSTUK

Cloud computing has attracted significant interest due to the increasing service demands from organizations offloading computationally intensive tasks to ***,datacenter infrastructure comprises hardware resources that consume high amount of energy and give out carbon emissions at hazardous *** cloud datacenter,Virtual Machines(VMs)need to be allocated on various Physical Machines(PMs)in order to minimize resource wastage and increase energy *** allocation problem is *** finding an exact solution is complicated especially for large-scale *** this con text,this paper proposes an Energy-oriented Flower Pollination Algorithm(E-FPA)for VM allocation in cloud datacenter environments.A system framework for the scheme was developed to enable energy-oriented allocation of various VMs on a *** allocation uses a strategy called Dynamic Switching Probability(DSP).The framework finds a near optimal solution quickly and balances the exploration of the global search and exploitation of the local *** considers a processor,storage,and memory constraints of a PM while prioritizing energy-oriented allocation for a set of *** performed on MultiRecCloudSim utilizing planet workload show that the E-FPA outperforms the Genetic Algorithm for Power-Aware(GAPA)by 21.8%,Order of Exchange Migration(OEM)ant colony system by 21.5%,and First Fit Decreasing(FFD)by 24.9%.Therefore,E-FPA significantly improves datacenter performance and thus,enhances environmental sustainability.

关键词： virtualization green computing cloud datacenter energy optimization

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Optimizing the Synthesis of Ethyl tert-Butyl Ether in Continuous Catalytic Distillation Column Using New Ion Exchange Resin Catalyst

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Chinese Journal of Chemical Engineering 2013年第10期21卷 1121-1128页

作者： Muhammad Umar Yahia abubakar AI-Hamed abdulraheem AI-Zahrani Hisham Saeed Bamufleh Department of Chemical and Materials Engineering Faculty of Engineering King Abdul Aziz University Jeddah80204 Kingdom of Saudi Arabia

Liquid phase synthesis of one of the important fuel oxygenate, ethyl tert-butyl ether （ETBE）, from etha-nol and tert-butyl alcohol （TBA） has been studied in catalytic distillation column （CDC） using ion exchange resin catalyst CT-145H. A packed CDC of 1.2 m height and 50 mm diameter with indigenously developed reactive sec-tion packing was used to generate experimental data. Effect of different key variables on product purity in distillate, was investigated to find the optimum operating conditions for ETBE synthesis. The optimum conditions for 0.2 kg·s-1 of ethanol feed were found：reboiler duty of 375 W, molar feed ratio of 1︰1.3 of reactants, and reflux ratio of 7. Concentration profiles for each component along each column section at optimum conditions were also drawn. Neither output nor input multiplicity was observed at experimental conditions.

关键词： ethyl tert-butyl ether synthesis catalytic distillation ion exchange resin catalyst packed column mul-tiplicity

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