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Schwann-like adipose-derived stem cells as a promising therapeutic tool for peripheral nerve regeneration:effects of cholinergic stimulation

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Neural Regeneration Research 2021年第6期16卷 1218-1220页

作者： Roberta Piovesana Alessandro Faroni Ada Maria Tata adam j.reid Department of Biology and Biotechnologies“Charles Darwin” SapienzaUniversity of RomeRomeItaly Blond McIndoe Laboratories Division of Cell Matrix Biology and Regenerative MedicineSchool of Biological SciencesFaculty of BiologyMedicine and HealthThe University of ManchesterManchester Academic Health Science CentreManchesterUK Research Centre of Neurobiology“Daniel Bovet” SapienzaUniversity of RomeRomeItaly Department of Plastic Surgery&Burns Wythenshawe HospitalManchester University NHS Foundation TrustManchester Academic Health Science CentreManchesterUK

Schwann-like adipose-derived stem cells and nerve injury:Peripheral nerve injuries(PNIs)are a common clinical problem usually as a consequence of *** optimal surgical management,PNI has a lifelong impact on function and wellbeing of the *** peripheral nervous system(PNS)has regenerative capability,in contrast to the central nervous system(CNS),and is dependent on the plasticity of the peripheral glia,Schwann cells(SCs).Despite this regenerative capability,PNI recovery of sensorial and motor function is always incomplete causing pain,cold intolerance,paralysis and impairment of activities of daily living.

关键词： stimulation injuries paralysis

Gene expression changes in dorsal root ganglia following peripheral nerve injury: roles in inflammation,cell death and nociception

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Neural Regeneration Research 2019年第6期14卷 939-947页

作者： Sarah L.Martin adam j.reid Alexei Verkhratsky Valerio Magnaghi Alessandro Faroni Blond McIndoe Laboratories School of Biological SciencesUniversity of Manchester University Hospital of South Manchester Department of Plastic and BurnsManchester Faculty of Biology Medicine and HealthUniversity of Manchester Department of Pharmacological and Biomolecular Sciences Università degli Studi di Milano

Subsequent to a peripheral nerve injury, there are changes in gene expression within the dorsal root ganglia in response to the damage. This review selects factors which are well-known to be vital for inflammation, cell death and nociception, and highlights how alterations in their gene expression within the dorsal root ganglia can affect functional recovery. The majority of studies used polymerase chain reaction within animal models to analyse the dynamic changes following peripheral nerve injuries. This review aims to highlight the factors at the gene expression level that impede functional recovery and are hence are potential targets for therapeutic approaches. Where possible the experimental model, specific time-points and cellular location of expression levels are reported.

关键词： Gene expression polymerase chain reaction dorsal root ganglia inflammation nociception cell death peripheral nerve injury Schwann cells satellite glial cells nerve regeneration

Autophagy is involved in cytotoxic effects of crotoxin in human breast cancer cell line MCF-7 cells

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Acta Pharmacologica Sinica 2007年第4期28卷 540-548页

作者： Paul reid Celtic Biotech LtdDublinIreland

Aim:To investigate the role of crotoxin (CrTX)-induced autophagy in the death ofMCF-7 cells,a caspase-3-deficient,human breast cancer cell ***:Cul-tured MCF-7 cells were treated with various doses of CrTX,a phospholipase A2(PLA2) isolated from the venom of the South American rattlesnake,Crotalusdurissus *** cytotoxicity of CrTX in the presence and absence ofcaspase inhibitors was measured with methyl thiazolyl tetrazolium (MTT) andlactate dehydrogenase (LDH) leakage *** activation of autophagy wasdetermined with transmission electron microscope and monodansylcadaverin(MDC) *** upregulation of lysosomal enzymes,the release of cyto-chrome c (cyto-c),and the nuclear translocation of the apoptosis inducing factor(AIF) were examined by immunoblotting and ***:CrTXinhibited the viability of MCF-7 cells in a dose-and time-dependent ***-activated autophagy was revealed by punctuate MDC labeling,and an increase inthe formation of autophagosomes as well as apoptosis,as evidenced by nuclearcondensation and *** activation of cathepsin B,D,and L,inaddition to the release of cytochrome c and the relocation of AIF into nuclei,wereobserved after CrTX *** inhibitors 3-methyladenine (3-MA),NH4Cl,and the pan-caspase inhibitor,Z-Val-Ala-Asp-fluoromethylketone (Z-Vad-fmk),attenuated CrTX-induced cell ***:An autophagic mecha-nism contributes to the apoptosis of MCF-7 cells induced by CrTX.

关键词： crotoxin autophagy apoptosis cytochrome c apoptosis inducing factor

Planting position and shade enhance native seedling performance in forest restoration for an endangered malagasy plant

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Plant Diversity 2019年第2期41卷 118-123页

作者： Cyprien Miandrimanana j.Leighton reid Tahiry Rivoharison Chris Birkinshaw Missouri Botanical Garden Madagascar Research and Conservation Program Center for Conservation and Sustainable Development Missouri Botanical Garden

The critically endangered tree Schizolaena tampoketsana is confined to a few diminished and degraded forest fragments on the Malagasy highlands. This habitat is vulnerable to loss due to frequent fires in the surrounding grassland that threaten to spread into the forest. One of these fragments is the focus a conservation project and here the managers aim to conserve S. tampoketsana by restoring its forest habitat to its former extent as evidenced by remnant woody plants. To inform this activity the survival and early-stage growth of seedlings of four locally native tree species were compared under contrasting conditions of proximity to the remaining forest and shade. After 12 months, seedlings of three species(Baronia taratana, Eugenia pluricymosa, Uapaca densifolia) survived better and experienced improved growth in height in grassland close to the existing forest rather than distant from it, and two survived better with shade rather than unshaded. A number of mechanisms could explain these results including reduced exposure to desiccating sunlight and winds and better soil and greater water availability close to the forest. The seedlings of one species(Nuxia capitata) survived well under all conditions. This study suggests that reforestation in these dry highlands is most feasible adjacent to remnant forest fragments and in microhabitats that minimize water loss, though young plants of some tree species may be capable of surviving in harsher conditions.

关键词： Grassland Fire Forest restoration Local environment Schizolaena tampoketsana

Surfactant protein A (SP-A) binds to phosphatidylserine and competes with annexin V binding on late apoptotic cells

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Protein & Cell 2010年第2期1卷 188-197页

作者： Anne jäkel Kenneth B.M.reid Howard Clark Department of Pharmacology University of OxfordMansfield RoadOxford OX13QTUK MRC Immunochemistry Unit Department of BiochemistryUniversity of OxfordSouth Parks RoadOxford OX13QUUK University of Southampton MP 803 Level FSouth BlockSouthampton General HospitalSouthampton SO166YDUK

The role of surfactant protein A(SP-A)in the recognition and clearance of apoptotic cells is well established,but to date,it is still not clear which surface molecules of apoptotic cells are involved in the *** we present evidence that phosphatidylserine(PS)is a relevant binding molecule for human *** binding is Ca^(2+)-dependent and is not inhibited by mannose,suggesting that the sugar-binding site of the carbohydrate recognition domain(CRD)of SP-A is not *** cytometry studies on apoptotic jurkat cells revealed apparent inhibition of annexin V binding by increasing concentrations of SP-A in late apoptotic but not early apoptotic cells,and this was consistent for jurkat cells and *** these data,confocal microscopy results show a co-localisation of annexin V and SP-A in late apoptotic but not early apoptotic ***,we cannot conclude that this inhibition is exclusively due to the binding of SP-A to PS on the cell surface,as annexin V is not wholly specific for PS and SP-A also interacts with other phospholipids that might become exposed on the apoptotic cell surface.

关键词： surfactant proteins phosphatidylserine apoptotic cells

Control of the carrier-envelope phases of a synchronously pumped femtosecond optical parametric oscillator and its applications

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Chinese Science Bulletin 2008年第5期53卷 642-651页

作者： SUN jingHua Barry j S Gale Derryck T reid Ultrafast Optics Group School of Engineering and Physical Sciences Heriot-Watt University Edinburgh EH14 4AS UK Laboratory of Optical Physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China

The intrinsic synchronization, multi-color outputs and related carrier-envelope phases (CEP) among pulses bring advantages to synchronously pumped femtosecond optical parametric oscillators and the pumping sources for broadband frequency comb generation and ultrashort waveform coherent syn-thesis. In this paper, we discuss our latest research results in this field, which cover the following as-pects: the phase relationship and energy conservation law in an OPO and related experimental verifi-cation; control of the pumping Ti:sapphire femtosecond laser's CEP by self-referencing technology, and its repetition-rate locking by piezoelectric transducer (PZT); CEP locking of the pulses from the OPO by beating the non-phase-matched visible outputs against pump supercontinuum to obtain a driving signal for a fast PZT on the OPO end mirror; the generation of a broadband frequency comb spanning from 400 nm to 2.4 μm with 1.2 kHz bandwidth; and the realization of coherent interference between phase controlled pump pulses and signal second harmonic pulses.

关键词：光学参数振荡器载波包络相同步控制超短光脉冲

Potential for natural and enhanced attenuation of sulphanilamide in a contaminated chalk aquifer

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journal of Environmental Sciences 2017年第12期29卷 39-48页

作者： Karen A.Bennett Simon D.Kelly Xiangyu Tang Brian j.reid School of Environmental Sciences University of East AngliaNorwich Research ParkNorwich NR4 7TJUK Food and Environmental Protection Laboratory International Atomic Energy Agency1400 ViennaAustria Key Laboratory of Mountain Surface Processes and Ecological Regulation Institute of Mountain Hazards and EnvironmentChinese Academyof SciencesChengdu 610041China

Understanding antibiotic biodegradation is important to the appreciation of their fate and removal from the environment. In this research an Isotope Ratio Mass Spectrometry（IRMS）method was developed to evaluate the extent of biodegradation of the antibiotic,sulphanilamide, in contaminated groundwater. Results indicted an enrichment in δ^（13）C of8.44‰ from-26.56（at the contaminant source） to-18.12‰（300 m downfield of the source）.These results confirm reductions in sulphanilamide concentrations（from 650 to 10 mg/L）across the contaminant plume to be attributable to biodegradation（56%） vs. other natural attenuation processes, such as dilution or dispersion（42%）. To understand the controls on sulphanilamide degradation ex-situ microcosms assessed the influence of sulphanilamide concentration, redox conditions and an alternative carbon source. Results indicated, high levels of anaerobic capacity（~50% mineralisation） to degrade sulphanilamide under high（263 mg/L）, moderate（10 mg/L） and low（0.02 mg/L） substrate concentrations. The addition of electron acceptors; nitrate and sulphate, did not significantly enhance the capacity of the groundwater to anaerobically biodegrade sulphanilamide. Interestingly, where alternative carbon sources were present, the addition of nitrate and sulphate inhibited sulphanilamide biodegradation. These results suggest, under in-situ conditions, when a preferential carbon source was available for biodegradation, sulphanilamide could be acting as a nitrogen and/or sulphur source. These findings are important as they highlight sulphanilamide being used as a carbon and a putative nitrogen and sulphur source, under prevailing iron reducing conditions.

关键词： Antibiotics Sulphonamide Groundwater contamination Biodegradation Stable isotope fractionation IRMS

Diffusion of lithium ions in Lithium-argyrodite solid-state electrolytes

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npj Computational Materials 2020年第1期6卷 326-335页

作者： Ardeshir Baktash james C.reid Tanglaw Roman Debra j.Searles Centre for Theoretical and Computational Molecular Science Australian Institute for Bioengineering and NanotechnologyThe University of QueenslandSt Lucia 4072 QLDAustralia School of Mathematics and Physics The University of QueenslandSt Lucia 4072 QLDAustralia School of Physics The University of SydneyCamperdown 2006 NSWAustralia School of Chemistry and Molecular Biosciences The University of QueenslandSt Lucia 4072 QLDAustralia

The use of solid-state electrolytes to provide safer,next-generation rechargeable batteries is becoming more feasible as materials with greater stability and higher ionic diffusion coefficients are ***,accurate determination of diffusion coefficients in solids is problematic and reliable calculations are highly sought-after to understand how their structure can be modified to improve their *** this paper we compare diffusion coefficients calculated using nonequilibrium and equilibrium ab initio molecular dynamics simulations for highly diffusive solid-state electrolytes,to demonstrate the accuracy that can be ***,we show that ab initio nonequilibrium molecular dynamics can be used to determine diffusion coefficients when the diffusion is too slow for it to be feasible to obtain them using ab initio equilibrium simulations.

关键词： diffusion nonequilibrium feasible

Spatial distribution of soil hydraulic parameters estimated by pedotransfer functions for the jialing River Catchment,Southwestern China

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journal of Mountain Science 2016年第1期13卷 29-45页

作者： LEI Wen-juan TANG Xiang-Yu Brian j.reid ZHOU Xiang-Yang Key Laboratory of Mountain Surface Processes and Ecological Regulation Institute of Mountain Hazards and EnvironmentChinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China School of Environmental Sciences University of East AngliaNorwich NR4 7T JUK College of Water Resource&Hydropower University of SichuanChengdu 610065China

Soil hydraulic parameters θs,α,n,Ks,L and θr of the van Genuchten-Mualem model were estimated using three pedotransfer functions(PTFs) based on soil properties for surface soils of the largest main tributary catchment(the jialing River) of the upper Yangtze River in *** soil database was from the second national soil survey of China with a spatial 30 × 30 arc-second *** to the statistical analysis of the differences between the continuous-PTFs-estimated values of soil hydraulic parameters for the study catchment and the reference values for a specific texture class provided in the development database of a specific PTF,best estimations were obtained using the W?sten *** Rawls & Brakenssiek PTF was good estimation for parameter θr that was assumed as zero by W?sten *** established higher θr(0.08%) and lower Ks(20 cm/d) and θs(0.43%) in the mid-downstream area relative to the other areas of the catchment could lead to larger amounts of surface runoff andconsequently provide higher energy to erode ***,these factors provide a supporting explanation for previously reported severe soil erosion occurring in this *** heterogeneity analysis for estimated hydraulic parameters in terms of semivariogram showed that the spatial correlation distance was in the range of 50-80 km and that the spatial variability(sill) was not large except for parameters Ks and *** semi-variance with the exponential model at the zero distance(nugget) was 30%-50% of the *** study provided a practical PTF approach for estimating soil hydraulic properties from soil survey data at a large watershed *** estimation results could provide better insight into the mechanism of surface runoff and soil erosion,which is important to better understand and manage erosion in the catchment.

关键词： hydraulic catchment erosion downstream runoff texture exponential heterogeneity watershed fitted