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A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis

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Journal of Integrative Plant Biology 2021年第8期63卷 1462-1474页

作者： Xishi Zhou Junna He Christos n.Velanis Yiwang Zhu Yuhan He Kai Tang Mingku Zhu Lisa graser Erica de Leau Xingang Wang Lingrui Zhang W.Andy Tao Justin goodrich Jian-Kang Zhu Cui-Jun Zhang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518124 China College of Horticulture China Agricultural UniversityBeijing100193 China Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907 USA Institute of Molecular Plant Science School of Biological SciencesUniversity of EdinburghDaniel Rutherford BuildingMax Born CrescentEdinburghEH93BF United Kingdom Shanghai Center for Plant Stress Biology and CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesShanghai201602 China Institute of Integrative Plant Biology School of Life SciencesJiangsu Normal UniversityXuzhou221116 China University of Applied Sciences Mannheim Paul-Wittsack-Str.10Mannheim68163 Germany Department of Biochemistry Purdue UniversityWest LafayetteIN 47907 USA

In eukaryotes,histone acetylation is a major modification on histone n-terminal tails that is tightly connected to transcriptional ***6 is a histone deacetylase involved in the transcriptional regulation of genes and transposable elements(TEs)in Arabidopsis ***6 has been shown to participate in several complexes in plants,including a conserved SIn3 ***,we uncover a novel protein complex containing HDA6,several Harbinger transposon-derived proteins(HHP1,SAnT1,SAnT2,SAnT3,and SAnT4),and MBD domain-containing proteins(MBD1,MBD2,and MBD4).We show that mutations of all four SAnT genes in the sant-null mutant cause increased expression of the flowering repressors FLC,MAF4,and MAF5,resulting in a late flowering *** deep sequencing reveals that while the SAnT proteins and HDA6 regulate the expression of largely overlapping sets of genes,TE silencing is unaffected in sant-null *** global histone H3 acetylation profiling shows that SAnT proteins and HDA6 modulate gene expression through ***,our findings suggest that Harbinger transposon-derived SAnT domain-containing proteins are required for histone deacetylation and flowering time control in plants.

关键词： deacetylation flowering Harbinger HDA6 histone acetylation protein complex SAnT

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骨传导刺激产生的前庭诱发眼外电位

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世界核心医学期刊文摘（神经病学分册） 2005年第11期1卷 23-24页

作者： Rosengren S.M. McAngus Todd n.P. Colebatch J.g. 李一明 Institute of neurological Sciences UNSW Clinical School Prince of Wales Hospital Randwick Sydney NSW 2031 Australia

Objective: To investigate the origin, whether ocular or extraocular, of the short latency frontal potential (n15) reported by Todd et al. (2003) following vestibular stimulation. Methods: Fourteen subjects with low VEMP thresholds (VT) and 9 patients with vestibular or ocular disorders were stimulated at the mastoid with bone-conducted tone bursts (500 Hz, 8 ms) above vestibular threshold, using a B71 bone vibrator. Surface potentials were recorded from Fpz and around the eyes and referred to linked earlobes. Results: The n15 was present at Fpz,but was largest around the eyes (mean amplitude 2.6 μV, peak latency 13.4 ms, with stimulation at +18 dB above threshold) and was generally in phase above and below the eyes. The response was vestibular-dependent and modulated by alteration of gaze direction. The potentials were delayed in a patient with Miller Fisher syndrome and were larger in patients with superior canal dehiscence than in controls. Conclusions: We report a new vestibular-evoked extraocular potential. Its properties are not consistent with an eye movement. It is likely to be produced, mainly or exclusively, by synchronous activity in extraocular muscles (i.e. a myogenic potential). Significance: Vestibularevoked extraocular potentials extend the range of vestibular pathways that can be assessed electrophysiologically, and may be a useful additional test of vestibular function.

关键词：外电位前庭功能骨传导短纯音参考电极表面电位刺激器电生理学方法肌源性骨小管

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Oligomerized Amyloid-β1-40Peptide Favors Cholesterol, Oxysterol, and Fatty Acid Accumulation in Human neuronal SK-n-BE Cells

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International Journal of Clinical Medicine 2015年第11期6卷 813-824页

作者： Amira Zarrouk Thomas nury Mohamed Hammami gérard Lizard Laboratoire de Biochimie (LR12ES05)-Lab-nAFS “nutrition Aliments Fonctionnels et Santé Vasculaire” Faculté de Médecine Université de Monastir Monastir Tunisie Equipe “Biochimie du Peroxysome Inflammation et Métabolisme Lipidique” (EA 7270) Université de Bourgogne-Franche Comté/INSERM Dijon France

Amyloid peptide, the main component of senile plaques, is a major biological characteristic of Alzheimer’s disease (ad). The aim of the present study conducted on human neuronal SK-n-BE cells was to evaluate whether oligomerized Aβ1-40-induced cell damages was associated with lipid modifications. Under treatment with Aβ1-40 (10 - 100 μM;24 - 48 h), cell viability was recorded with the MTT test and by measuring LDH activity. Mitochondrial transmembrane potential and ATP production were assessed using flow cytometry and a luciferase-based ATP bioluminescence assay, respectively. Annexin V-CF647 staining assay for cell apoptosis detection was performed using flow cytometry. Potentially intracellular cytotoxic lipids (oxysterols: 7α-hydroxycholesterol (7α-OHC), 7β-hydroxycholesterol (7β-OHC), and 7-ketocholesterol (7KC), 24(S)-hydroxycholesterol;arachidonic acid (C20:4 n-6);VLCFAs (C22:0, C24:0, C24:6 and C26:0)) were measured using gas chromatography coupled with mass spectrometry. The cellular level of docosahexaenoic acid (C22:6 n-3), often altered in ad, was also quantified. In the presence of Aβ1-40, the percentage of MTT-positive cells decreased and was associated with an increase in LDH activity. In addition, treatment with oligomerized Aβ1-40 induced a decrease of mitochondrial transmembrane potential as well as an apoptotic cell death. Sterol analysis revealed a higher cholesterol level and a significant increase of cytotoxic oxysterols per cell (7KC + 7β-OHC), and of the [(7β-OHC + 7KC)/cholesterol] ratio, considered as a lipid peroxidation index, in Aβ1-40-treated cells. An enhancement of C20:4 n-6, C22:6 n-3 and saturated VLCFAs was also observed. Therefore, Aβ1-40-induced side effects are associated with intracellular accumulation of lipids, especially cholesterol, oxysterols (7β-OHC, 7KC), C20:4 n-6, and saturated VLCFAs, which could in turn contribute to neurotoxicity.

关键词： SK-n-BE Cells Oligomerized Aβ1-40 Cholesterol Oxysterols Very Long Chain Fatty Acids

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Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungi

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advances in Biological Chemistry 2013年第2期3卷 198-207页

作者： Priscilla M. Mfombep Zachary n. Senwo Omoanghe S. Isikhuemhen Department of Biological & Environmental Sciences Alabama gricultural and Mechanical University Normal USA Department of natural Resources & Environmental Design North Carolina A & T State University Greensboro USA

Beta-glucosidase is among the suite of enzymes produced by white rot fungi (WRF) to biodegrade plant biomass. This study investigated the enzymatic activities and kinetic properties of β-glucosidase from seventeen WRF comprised of the following species from various geographical locations: Pleurotus ostreatus, Auricularia auricular, Polyporus squamosus, Trametes versicolor, Lentinula edodes, and grifola frondosa. All the WRF studied showed β-glucosidase activities. Significant variations in protein and carbohydrate contents were also recorded. Beta-glucosidase activities after 30 min of incubation ranged from 6.4 μg (T. versicolor) to 225 μg (g. frondosa). The calculated kinetic constant (Km) ranged from 0.47 μM (A. auricular-1120) to 719 μM (L. edodes-7). The Vmax depending on the kinetic transformation model ranged from 0.21 μg·min-1 (T. versicolor) to 9.70 μg·min-1 (g. frondosa-28). Beta-glucosidase activities also exhibited pH optima between 3.5 and 5.0 while temperature optima were between 60°C and 70°C with some media exhibiting a secondary temperature peak at 90°C attributable to the presence of thermostable isoenzyme. WRF if appropriately screened and purified can be harnessed to potentially improve the bio-conversion of cellulose to glucose and also facilitate efficient plant biomass biodegradation and production of useful plant bio-products.

关键词： White Rot Fungi β-glucosidase EC 3.2.1.21 Enzymatic Activities Kinetic Properties Plant Biomass

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Mathematical Modelling of Mass Transfer and Free Convection Current Effects on Unsteady Viscous Flow with Ramped Wall Temperature

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World Journal of Mechanics 2011年第4期1卷 176-184页

作者： Marneni narahari Osman Anwar Bég Swapan Kumar ghosh 不详

An exact analysis of the flow of an incompressible viscous fluid past an infinite vertical plate is conducted taking into account the presence of foreign mass or constant mass flux and ramped wall temperature. The dimensionless governing coupled linear partial differential equations are solved using the Laplace transform technique. Two different solutions for the fluid velocity are obtained–one valid for the fluids of Schmidt numbers different from unity, and the other for which the Schmidt number is unity. The effects of Prandtl number (Pr), Schmidt number (Sc), time (t) and mass to thermal buoyancy ratio parameter (n) for both aiding and opposing buoyancy effects on the velocity and skin-friction are studied. Also, the heat and mass transfer effects on the flow near a ramped temperature plate have been compared with the flow near a plate with constant temperature.

关键词： natural Convection Vertical Plate Ramped Wall Temperature Heat and Mass Transfer

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0.005%拉坦前列素与0.03%比马前列素治疗原发性开角型青光眼的疗效评比

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世界核心医学期刊文摘（眼科学分册） 2005年第7期1卷 36-37页

作者： Konstas A.g.P. Katsimbris J.M. Lallos n. W.C. Stewart 杨秀梅 Pharmaceutical Research network LLC 1 Southpark Circle Charleston SC 29407 United States

Objective: To evaluate latanoprost versus bimatoprost given each evening over the 24- hour diurnal curve. Design: Double- masked, 2- center, crossover comparison. Participants: Forty- two of 44 patients with primary open- angle glaucoma (POAg) completed the study. Methods: Consecutive patients were not treated during a baseline 24- hour curve after a glaucoma medicine- free period. They then were randomized to either latanoprost or bimatoprost for a 7- week treatment period. Diurnal curve intraocular pressures (IOPs) were measured at treatment period end at 2 AM, 6 AM, 10 AM, 2 PM, 6 PM, and 10 PM. After the first treatment period, patients were changed to the opposite medicine without a medicine- free period. Diurnal curve measurements were performed again at the end of the second 7- week treatment period. Main Outcome Measure: The 24- hour diurnal IOP. Results: On the last day of treatment, mean 24- hour IOPs were 17.3± 2.8 mmHg for latanoprost and 16.7± 2.4 mmHg for bimatoprost (P=0.01). The 6 PM individual time point for IOP was statistically lower for bimatoprost after a Bonferroni correction (P=0.008). The largest IOP difference at any time point was 0.9 mmHg at 6 PM. The most common side effect was conjunctival hyperemia, which occurred less with latanoprost (n=6) than with bimatoprost (n= 15) (P=0.004). Two patients had their treatments discontinued while on bimatoprost, one due to conjunctival hyperemia and the other due to ocular intolerance. Conclusion: This study indicates that the 24- hour diurnal IOP is statistically lower in POAg with bimatoprost, compared with latanoprost, among patients who tolerated bimatoprost. However, the IOP difference between groups was small and may not be clinically meaningful. In contrast, conjunctival hyperemia seems statistically greater with bimatoprost. The exact clinical importance of conjunctival hyperemia, if any, needs to be clarified further.

关键词：比马前列素拉坦抗青光眼药物结膜充血停药期基线测量测量时间最后一天

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影像专家作为诊断和介入影像方面系统性综述作者的角色及其对科学素质的影响:来自欧洲AIM循证医学放射学工作组的报告

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国际医学放射学杂志 2014年第5期 486-487页

作者： F.Sardanelli H.Bashir D.Berzaczy g.Cannella A.Espeland n.Flor 罗松 Department of Biomedical Sciences for Health Università degliStudi di MilanoPiazza E.Malan

摘要目的评估关于影像和影像引导诊断过程的文章,且作者是放射学医师或核医学医生(影像专家)的系统综述,确定影像专家的存在对系统性综述整体质量的影响。材料与

关键词：影像专家系统综述质量核医学原子医学 AIM 系统性综述放射学

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高分辨率螺旋CT冠状动脉造影对拟行诊断性常规冠状动脉造影患者的应用价值

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世界核心医学期刊文摘(心脏病学分册) 2006年第3期 43-44页

作者： Mollet n.R. Cademartiri F. Van Mieghem C.A.g. P.J. De Feyter 马超 Erasmus Medical Center Thoraxcenter Dr Molewaterplein 40 3015GD Rotterdam Netherlands Dr.

Background-The diagnostic performance of the latest 64-slice CT scanner, with increased temporal(165 ms) and spatial(0.4 mm3) resolution, to detect significant stenoses in the clinically relevant coronary tree is unknown. Methods and Results -We studied 52 patients(34 men; mean age, 59.6± 12.1 years)with atypical chest pain, stable or unstable angina pectoris, or non-ST-segment elevation myocardial infarction scheduled for diagnostic conventional coronary angiography. All patients had stable sinus rhythm. Patients with initial heart rates ≥ 70 bpm received β -blockers. Mean scan time was 13.3± 0.9 seconds. The CT scans were analyzed by 2 observers unaware of the results of invasive coronary angiography, which was used as the standard of reference. All available coronary segments, regardless of size, were included in the evaluation. Lesions with ≥ 50 luminal narrowing were considered significant stenoses. Invasive coronary angiography demonstrated the absence of significant disease in 25% (13 of 52), single-vessel disease in 31% (16 of 52), and multivessel disease in 45% (23 of 52) of patients. One unsuccessful CT scan was classified as inconclusive. ninety-four significant stenoses were present in the remaining 51 patients. Sensitivity, specificity, and positive and negative predictive values of CT for detecting significant stenoses on a segment-by-segment analysis were 99% (93 of 94; 95% CI, 94 to 99), 95% (601 of 631; 95% CI, 93 to 96), 76% (93 of 123; 95% CI, 67 to 89), and 99% (601 of 602; 95% CI, 99 to 100), respectively. Conclusions -noninvasive 64-slice CT coronary angiography accurately detects coronary stenoses in patients in sinus rhythm and presenting with atypical chest pain, stable or unstable angina, or non-ST-segment elevation myocardial infarction.

关键词：冠状动脉造影冠脉造影心血管造影患者诊断性 CT 常规

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Structural and Magnetic Properties of Cr3+ Doped Mg Ferrites

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World Journal of Condensed Matter Physics 2012年第1期2卷 27-35页

作者： Faizun nesa A. K. M. Zakaria M. A. Saeed Khan S. M. Yunus A. K. Das S.-g. Eriksson M. n. I. Khan M. A. Hakim Bangladesh Atomic Energy Commission Dhaka Bangladesh Chalmers University of Technology Gothenburg Sweden Jahangirnagar Universiry Dhaka Bangladesh

The polycrystalline MgCrxFe2-xO4 ferrites (0.0 ? x ? 1.0) were prepared by conventional solid state ceramic sintering technique in air at 1300?C. X-ray diffraction experiments were carried out on all the samples in order to characterize the materials at room temperature. The X-ray diffraction patterns showed sharp peaks indicating the formation of single phased cubic spinel structure. The lattice parameters of the samples were determined from the X-ray diffraction data using nelson-Riley extrapolation method. It was found that the lattice parameter decreased with increasing Cr concen- tration obeying Vegard’s law. Magnetic properties of the samples were measured using an Impedance Analyzer. Real and imaginary parts of the complex permeability, loss factor and quality factor were measured as the function of frequency at three different sintering temperatures 1250?C, 1300?C and 1350?C for all the samples in the frequency range 1 kHz to 13 MHz. Frequency stability of the real part of permeability increases with increasing Cr concentration and also with sintering temperature. Imaginary part of permeability decreases with increasing frequency and increased with increasing both of the Cr content and sintering temperature. Loss factor decreased with increasing frequency while the quality factor (Q) increased with increasing frequency for all the samples. The temperature de- pendence of initial permeability was measured for all the samples sintered at 1300?C. The Curie temperature (Tc) was determined from the -T curves. The values of Tc were found to be 733 K, 657 K, 583 K, 468 K, 400 K and 317 K for x = 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0, respectively.

关键词： Ferrites Spinel Structure Magnetic Properties Permeability Loss Factor Curie Temperature

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宽幅打印机中的墨滴运动之气流研究

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中国印刷与包装研究 2009年第1期1卷 100-101页

作者： Author n. Link S. Lampert R. gurka A. Liberzon g. Hetsroni R. Semiat Wolfson Faculty of Chemical Engineering Technion-Israel Institute of Technology 32000 Haifa＇israel Faculty of Mechanical Engineering Technion-Israel Institute of Technology 32000 Haifa Israel

高质量喷墨打印技术正越来越多应用于各类生产中。本文主要研究喷墨打印头的相对运动及在打印头和印刷介质间产生的气流对喷射过程中墨滴的影响和对印刷精度的影响。我们通过实验对气流进行观测。由于实验中墨流的关键区域较难测量,因... 详细信息

高质量喷墨打印技术正越来越多应用于各类生产中。本文主要研究喷墨打印头的相对运动及在打印头和印刷介质间产生的气流对喷射过程中墨滴的影响和对印刷精度的影响。我们通过实验对气流进行观测。由于实验中墨流的关键区域较难测量,因此采用CFD模拟程序来了解油墨流动状态,从而了解墨流对印刷质量的影响。实验结果与线性剪切流仅因实验误差产生微量偏差。另外,实验结果还表明,在不引起气流大幅度变化的情况下,适当加大打印头和印刷介质间的相对运动是可以接受的。

关键词：喷墨打印 PIV 气流墨滴运动

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