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Immunoprophylaxis failure and vaccine response in infants born to mothers with chronic hepatitis B infection in Djibouti

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World Journal of Hepatology 2024年第7期16卷 1039-1050页

作者： Sahal Darar Dirir Ambroise D Ahouidi aboubacry drame Warsama Osman Abdi Guelleh Youssouf Kayad Mohamed Houmed Aboubakar Makhtar Camara Coumba Toure Kane Halimatou Diop Ndiaye Laboratoire Medical de la Caisse National de SécuritéSocial de Djibouti Caisse National de SécuritéSocial de DjiboutiDjibouti BP 696Senegal Parasitology Institut de Recherche en santéde Surveillanceépidémiologique et de FormationDakar 7325Senegal Ecole Doctoral UniversitéAlioune Diop de BambeyDakar 7325Senegal Department of Des Soins Caisse National de Securite SocialDjibouti 696Senegal Centre de Soin Caisse National de Securite SocialDjibouti 696Senegal Laboratoire Medical Mer Rouge Djibouti 99399Senegal Centre Hospitalier Universitaire Aristide le Dantec Laboratoire Bactériologie-Virologie-Hôpital Aristide le DantecDakar 7325Senegal Department of Virology Institut de Recherche en Santéde Surveillanceépidémiologique et de FormationDakar 7325Senegal Bacteriology and Virology Laboratory Le Dantec University Teaching HospitalDakar BP 7325Senegal

BACKGROUND In endemic areas,vertical transmission of hepatitis B virus(HBV)remains a major source of the global reservoir of infected *** mother-to-child transmission(MTCT)of HBV is at the heart of World Health Organization’s goal of reducing the incidence of HBV in children to less than 0.1%by *** screening for hepatitis B during pregnancy and neonatal vaccination are the main preventive *** To evaluate the efficacy of HBV vaccination combined with one dose of immunoglobulin in children born to hepatitis B surface antigen(HBsAg)-positive mothers in Djibouti *** We conducted a study in a prospective cohort of HBsAg-positive pregnant women and their *** study ran from January 2021 to May 2022,and infants were followed up to 7 mo of *** serological markers and viral load in pregnant women were measured using aVidas microparticle enzyme-linked immunosorbent assay(Biomérieux,Paris,France)and the automated Amplix platform(Biosynex,Strasbourg,France).All infants received hepatitis B immunoglobulin and were vaccinated against HBV at *** infants were closely monitored to assess their seroprotective response and for failure of *** logistic regression was also used to identify risk factors associated with immunoprophylaxis failure and poor vaccine *** statistical analyses were performed with version 4.0.1 of the R *** Of the 50 pregnant women recruited,the median age was 31 years,ranging from 18 years to 41 *** MTCT rate in this cohort was 4%(2/50)in HBsAg-positive women and 67%(2/3)in hepatitis B e antigen-positive women with a viral load>200000 IU/*** the 48 infants who did not fail immunoprophylaxis,8(16%)became poor responders(anti-HB100 mIU/mL).Factors associated with this failure of immunoprophylaxis were maternal HBV DNA lev

关键词： Hepatitis B surface antigen Infants Hepatitis B immunoglobulin Hepatitis vaccine Djibouti

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High-dimensional supervised classification in a context of non-independence of observations to identify the determining SNPs in a phenotype

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Infectious Disease Modelling 2023年第4期8卷 1079-1087页

作者： aboubacry Gaye Abdou Ka Diongue Lionel Nanguep Komen Amadou Diallo Seydou Nourou Sylla Maryam Diarra Cheikh Talla Cheikh Loucoubar Laboratory for Studies and Research in Statistics and Development Gaston Berger University of Saint LouisSenegal Epidemiology Clinical Research and Data Science UnitInstitute Pasteur de Dakar220DakarSenegal African Institute for Mathematical Sciences(AIMS) Senegal Information and Communication Technologies for Development Alioune Diop University of BambeySenegal

This work addresses the problem of supervised classification for highly correlated highdimensional data describing non-independent observations to identify SNPs related to a *** use a general penalized linear mixed model with a single random effect that performs simultaneous SNP selection and population structure adjustment in highdimensional prediction ***,the model simultaneously selects variables and estimates their effects,taking into account correlations between *** nucleotide polymorphisms(SNPs)are a type of genetic variation and each SNP represents a difference in a single DNA building block,namely a *** research has shown that SNPs can be used to identify the correct source population of an individual and can act in isolation or simultaneously to impact a *** this regard,the study of the contribution of genetics in infectious disease phenotypes is of great *** this study,we used uncorrelated variables from the construction of blocks of correlated variables done in a previous work to describe the most related observations of the *** model was trained with 90%of the observations and tested with the remaining 10%.The best model obtained with the generalized information criterion(GIC)identified the SNP named rs2493311 located on the first chromosome of the gene called PRDM16((PR/SET domain 16))as the most decisive factor in malaria attacks.

关键词： Non independence of observations Correlated variables High-dimensional supervised classification SNP Phenotype

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In VitroGermination and Early Vegetative Growth of Five Tomato (Solanum lycopersicumL.) Varieties under Salt Stress Conditions

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American Journal of Plant Sciences 2021年第10期12卷 796-817页

作者： Abdou Khadre Sané Bassirou Diallo aboubacry Kane Maurice Sagna Djibril Sané Mame Ourèye Sy Laboratoire Campus de Biotechnologies végétales Département de Biologie Végétale Faculté des Sciences et Techniques Université Cheikh Anta Diop de Dakar Dakar Sénégal Laboratoire Mixte International-Adaptation des Plantes et Microorganismes associés aux Stress Environnementaux (LMI-LAPSE) IRD ISRA UCAD Dakar Sénégal Laboratoire National de Recherches sur les Productions Végétales (LNRPV) Unité de recherche en culture in vitro (URCI) Institut Sénégalais de Recherches Agricoles (ISRA) Dakar Sénégal

In Senegal, tomato (Solanum lycopersicum L.) cultivation is affected by salinity in many agro-ecological zones. The selection of salt tolerant varieties would be an alternative solution to enhance the production. Thus, germination and growth are studied under axenic conditions for five varieties of tomato subjected to increasing concentrations of NaCl [0, 35, 70 and 105 mM], and supplemented in an MS/2 medium for 30 days. The results reveal that salt negatively affects the evaluated parameters. The Rodeo and Lady Nema varieties have the lowest final germination rates (50%) unlike the Mongal variety (55%). These last two varieties have a decrease of 71.78% and 81.28% in the height of the stem, respectively, in the presence of NaCl at [105 mM] while that of the Rodeo variety is 70%. The Xewel variety has the greatest average number of leaves in the presence of [NaCl 35 mM] (4.95 leaves) and [NaCl 70 mM] (4.77 leaves). The Lady Nema variety records the longest taproot length (2.99 cm) unlike the Rodeo variety (2.25 cm) at [NaCl 105 mM]. The Ganila variety reveals the highest number of secondary roots at the concentrations of [0, 35 and 105 mM] of NaCl with, respectively, 4

关键词： Solanum lycopersicum NaCl Germination Growth Tolerance In Vitro Conditions

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Response to Inoculation with Arbuscular Mycorrhizal Fungi of Two Tomato (Solanum lycopersicum L.) Varieties Subjected to Water Stress under Semi-Controlled Conditions

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Agricultural Sciences 2022年第12期13卷 790-819页

作者： Abdou Khadre Sané Bassirou Diallo aboubacry Kane Mariama Ngom Maïmouna Cissoko Mame Ourèye Sy Laboratoire Campus de Biotechnologies Végétales Département de Biologie Végétale Faculté des Sciences et Techniques Université Cheikh Anta Diop de Dakar Dakar Sénégal Laboratoire Mixte International-Adaptation des Plantes et Microorganismes associés aux Stress Environnementaux (LMI-LAPSE) IRD ISRA UCAD Dakar Sénégal Laboratoire National de Recherches sur les Productions Végétales (LNRPV) Unité de recherche en culture in vitro (URCI) Institut Sénégalais de Recherches Agricoles (ISRA) Dakar Sénégal Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD Centre de Recherche de Bel Air Dakar Senegal

In arid and semi-arid regions, the growth and development of cultivated plants, especially tomato (Solanum lycopersicum L.), are severely limited by water deficit. Thus, to cope with this constraint, the plant establishes symbiotic relationships with arbuscular mycorrhizal fungi (AMF) in the soil whose extension of the hyphae allows a better and deeper exploration;this notably improves the hydromineral nutrition of the plant. Therefore, the choice of fungal partner becomes crucial for the establishment of a crop in water-deficient soil. In this context, the contribution of AMF to the water stress tolerance of two varieties of tomato plants was assessed under semi-controlled conditions. Parameters, such as the mycorrhizal frequency, intensity of mycorrhization, relative mycorrhizal dependency, growth, and biochemical parameters (carbon, nitrogen, phosphorus, and proline contents) of plants subjected to three levels of water stress (T100, T70, and T30), were evaluated. The highest frequencies and intensities of mycorrhization and relative mycorrhizal dependencies were obtained with plants of the Xewel variety inoculated with Rhizophagus fasciculatus (F: 95.24%, 88.35%, and 13.64%;M: 40.52%, 37.52%, and 11.22%;D: 23.7%, 54.4%, and 78.82%) and in those of the Lady Nema variety inoculated with Claroideoglomus etunicatum (F: 95.12%, 87.01%, and 15.25%;M: 40.66%, 37.99%, and 11.42%;D: 19.27%, 57.01%, and 70.98%), respectively at water regimes of T100, T70 and T30. These same symbiotic couples recorded, at T30, the best survival rates (+ 40%) and the higher aerial (77% and 74%) and root dry weights (80% and 59%). Plants of the Xewel variety inoculated with R. fasciculatus recorded the highest contents of carbon (T70: 30.59% and T30: 21.55%) and phosphorus (T70: 0.18% and T30: 0.17%). Plants of the Lady Nema variety recorded the highest nitrogen contents with 3.51% and 3.20%, respectively at T70 and T30. Plants of the Lady Nema variety, inoculated with C. etunicatum, also re

关键词： Solanum lycopersicum Water Stress Arbuscular Mycorrhizal Fungi Growth Carbon Nitrogen Phosphorus Proline Tolerance

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Effect of Arbuscular Mycorrhizal Fungal Inoculation on Sorghum bicolor Growth at Different Phosphate Levels: A Greenhouse Study

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Journal of Agricultural Science and Technology(B) 2019年第4期9卷 234-240页

作者： Souleymane Kone Fallaye Kante Ibou Diop Fatou Ndoye Abdalla Gamby Dieudhiou aboubacry Kane Ousmane Sacko Inamoud Ibny Yattara Laboratory of Soil Microbiology (LMS) Faculty of Sciences and TechnicsUniversity of Sciences Technics and Technologies of Bamako(USTTB)BP E 3206Mali Department of Biology Vegetable Faculty of Sciences and TechnicsUniversity Cheikh Anta DiopBP 5005 Dakar-FannSenegal Common Laboratory of Microbiology Development Research InstituteSenegal Agronomic Research InstituteUniversity Cheikh Anta Diop(LCM/IRD/ISRA/UCAD)DakarSenegal

In sub-Saharan Africa, Sorghum (Sorghum bicolor) is an important cereal for both human being and animals. Unfortunately, its production is confronted to soils with deficiency of phosphorus. Traditional use of mineral phosphate on this culture fertilization is expensive and may cause contamination. It is thus necessary to seek more efficient and economic reasonable techniques to improve sorghum growth. Arbuscular mycorrhizal fungi (AMF) constitute a reference for phosphorus improvement and plant nutrition. This study aimed to investigate the effects of AMF strains (Rhizophagus irregulare, Glomus aggregatum, G. mosseae) on growth of sorghum cultivated in greenhouse on Sangalkam soil (Senegal) sterilized with or without Tilemsi natural phosphate (PNT). The phosphorus can represent until 0.2% of the dry weight of the plant. Two fertilizers were used separately and together to doses of 20 g by strain, 100 mg and 200 mg of PNT. The experiment lasted for 120 d. Results showed that mycorrhizal colonization intensity varied between 40% and 80% for all treatments. AMF inoculation increased sorghum plant height and biomass, regardless of PNT amendment. The inoculation permits to bring strain of AMF that intervene efficiently in the transportation and the availability of phosphorus for the plant.

关键词： Arbuscular mycorrhizal fungi Tilemsi natural phosphate Sorghum bicolor

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Inoculation with arbuscular mycorrhizal fungi improves seedlings growth of two sahelian date palm cultivars (Phoenix dactyliferaL., cv. Nakhla hamra and cv. Tijib) under salinity stresses

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Advances in Bioscience and Biotechnology 2014年第1期5卷 64-72页

作者： Ibrahima Léopold Djitiningo Diatta aboubacry Kane Codjo Emile Agbangba Maurice Sagna Diegane Diouf Frédérique Aberlenc-Bertossi Yves Duval Alain Borgel Djibril Sane IRD Institut de Recherche pour le Développement Montpellier France Laboratoire Campus de Biotechnologies Végétales Département de Biologie Végétale Faculté des Sciences et Techniques Un-iversité Cheikh Anta Diop Dakar Sénégal Laboratoire Campus de Biotechnologies Végétales Département de Biologie Végétale Faculté des Sciences et Techniques Un-iversité Cheikh Anta Diop Dakar Sénégal Laboratoire Commun de Microbiologie IRD/ISRA/UCAD Route des Hydrocarbures Dakar Sénégal Laboratoire d’Ecologie et d’Ecohydrologie Département de Bi-ologie Végétale Faculté des Sciences et Techniques Université Cheikh Anta Diop Dakar Sénégal

This study presents an analysis of the impact of mycorrhizal inoculation on growth under salt stress of date palms cultivars Nakhla hamra (NHH) and Tijib known in the Sahel for their earliness in flowering and fruiting. The seedlings were grown in a greenhouse on a sandy substrate watered to field capacity every two days and were subjected to increasing levels of NaCl (0, 1, 2, 4, 6, 8 and 16 g·L-1) and then inoculated with 5 strains of Glomus (G. aggregatum, G. intraradices, G. verriculosum, G. mosseae, G. fasciculatum). The experimental design was a randomized complete block with three factors (cultivars: Tijib and Nakhla Hamra x seven levels of NaCl concentrations x six levels of inoculum). The results showed that in the absence of NaCl, G. verriculosum significantly enhanced shoot growth: 33.5 cm against 30.3 cm in the control and roots growth: 81.5 cm against 78 cm in NHH, while in Tijib, the growth is stimulated by Glomus fasciculatum: 33.8 cm against 32.7 cm for stems and 90 cm against 86 cm for the roots of inoculated plants and controls. In contrast, in the presence of NaCl 8 g·L-1, NHH has a better growth in the presence of G. intraradices: 37.3 cm against 30.6 cm for stems and 77 cm against 73 for roots, while Tijib grows better in the presence of G. fasciculatum with respectively 31.9 cm against 31.7 cm and 51.27 cm against 51.6 cm for stems and roots of inoculated plants and controls. Biochemical analysis revealed that changes in levels of proline depend on the cultivar, the mycorrhizal strain used and concentrations of NaCl applied. These results open the prospect of using mycorrhizal fungi to improve the productivity of palm trees in the Sahel.

关键词： Sahel Date Palm Salinity Inoculation Mycorrhizae Glomus Proline

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RETRACTED:Effect of Long-Term Inorganic Fertilization on Diversity and Abundance of Bacterial and Archaeal Communities at Tillage in Irrigated Rice Field

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Advances in Bioscience and Biotechnology 2023年第1期14卷 18-33页

作者： Donald Tchouomo Dondjou Henri Fankem Abdala Gamby Diedhiou Marie-Thérèse Mofini Daouda Mbodj Sarah Pignoly Baboucarr Manneh Laurent Laplaze aboubacry Kane Victor Désiré Taffouo Department of Plant Biology Faculty of Science University of Douala (UDLA) Douala Cameroon Centre d’Etude Régional pour l’Adaptation à la Sécheresse (CERAAS) Institut Sénégalais de Recherches Agricoles (ISRA) Route de Khombole Thiès Sénégal Département de Biologie Végétale Faculté des Sciences et Techniques Université Cheikh Anta Diop (UCAD) Dakar Fann Sénégal Laboratoire Commun de Microbiologie (LCM) Centre de Recherche de Bel-Air Dakar Sénégal Laboratoire Mixte International Adaptation des Plantes et Microorganismes associés aux Stress Environnementaux (LAPSE) Centre de Recherche de Bel-Air Dakar Sénégal DIADE Université de Montpellier Montpellier France Centre d’Excellence Africain en Agriculture Environnement Santé et Sociétés (CEA-AGIR) UCAD Dakar Sénégal Centre d’Excellence Africain en Agriculture pour la Sécurité Alimentaire et Nutritionnelle (CEA-AGRISAN) UCAD Dakar Sénégal Africa Rice Center (AfricaRice) Saint-Louis Sénégal

Short Retraction Notice The paper does not meet the standards of "Advances in Bioscience and Biotechnology". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. The aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. Editor guiding this retraction: Prof. Abass Alavi (EiC of ABB). Please see the article page for more details. The full retraction notice in PDF is preceding the original paper which is marked "RETRACTED".

关键词： Inorganic Fertilization Soil Microbiome Tillage Next-Generation Sequencing 16S rRNA Gene V4 Region Senegal

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The Acute Angle Closure Crisis in Senegalese Melanodermas: Epidemiological and Clinical Aspects of 24 Cases

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Open Journal of Ophthalmology 2023年第4期13卷 407-412页

作者： Sy El Hadji Malick Aw Aissatou Soda Mbaye Mouhamed Ndiaye Aly Mbara Ka Jean Pierre Diagne aboubacry Sadikh Sow Joseph Matar Mass Ndiaye Paule Aida Ndoye Papa Amadou Ndiaye AbassNdao University Hospital Center Dakar Senegal Aristide Le Dantec University Hospital Center Dakar Senegal

Introduction Acute angle closure crisis (AACC) is a major ophthalmic emergency. It is a sight-threatening pathology that is very common in women but rarely described in melanodermas. The aim of this work is to investigate the epidemiological and clinical aspects of acute angle closure crisis. Results We collected 24 patient records, totaling 25 eyes. The average age of our patients was 52.04 years, with a male-to-female ratio of 0.6. A family history of glaucoma occurred in 34% of patients. Patients consulted for a painful red eye associated or not with decreased visual acuity (DVA). The condition was bilateral in one patient. The average visual acuity wax 0.34/10e. The cornea was cloudy and the pupil in areflexic mydriasis in all sick eyes. Hypothalamia was present in 72% of eyes and cataracts in 54.16% of cases. The average intraocular press was 44.38 mmHg and the average cup/disc (C/D) was 0.46. Gonioscopy was performed in 7 patients. The fellow eye did not present any abnormalities in 92% of cases. Conclusion The acute angle closure crisis is a rare condition in melanodermas and common in woman. His diagnostic is essentially clinical and completed by gonioscopy which plays a fundamental role. .

关键词： Acute Crisis Angle Hypertonia Gonioscopy

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Phenotypical Aspects of a Familial Syndromic Retinitis Pigmentosa

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Open Journal of Ophthalmology 2024年第2期14卷 168-173页

作者： Irma Eneida Dos Santos Joseph Matar Mass Ndiaye Mariama Diambone Badji Alioune Abdoulaye Ndongo Gerauld Akpo Jean Pierre Diagne Gabriel Karold Mendy Mouhamed Attye aboubacry Sadikh Sow Elhadji Amadou Ba Paule Aida Ndoye Roth Ophthalmology Department of Aristide Le Dantec Hospital Dakar Senegal Pediatric Department of Aristide Le Dantec Hospital Dakar Senegal Radiological Imaging Department of Aristide Le Dantec Hospital Dakar Senegal Ophthalmology Department of Abass Ndao Hospital Dakar Senegal

Aim: To report a familial case of syndromic retinitis pigmentosa identified at Aristide Le Dantec Hospital in Dakar and to describe their clinical characteristics ophthalmic. Observation: We report a sibling group of nine children, four died at a young age from unknown causes. Three children were affected by retinitis pigmentosa, two cases were syndromic. A history of nyctalopia was found in all three affected children. The mean age of onset of decreased visual acuity was 6.6 years. Patient 1 affected by syndromic retinitis pigmentosa had an extraocular sign of cystic dilation of the main bile duct. Patient 2 had myoclonic epilepsy, psychomotor retardation, and the molar tooth sign on cerebral MRI (highly suggestive of Joubert syndrome). The third child had isolated retinitis pigmentosa. Ophthalmological examinations (fundus examination, electroretinogram, and visual evoked potentials) and pediatric examinations in the remaining two children were normal. Discussion and Conclusion: Retinitis pigmentosa is a rare degenerative disease that can be associated with several other malformations, highlighting the importance of screening for associated conditions. It presents a grim functional prognosis and a life prognosis dependent on extraocular manifestations. Molecular biology (karyotyping, next-generation sequencing) could have identified the implicated genes and allowed for a formal diagnosis and genetic counseling.

关键词： Retinitis Pigmentosa Syndromic Heredity Ciliopathy

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Eyelid Wounds: Epidemiological, Clinical and Etiological Aspects

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Open Journal of Ophthalmology 2024年第1期14卷 55-62页

作者： Aly Mbara Ka Jean Pierre Diagne Aissatou Aw El Hadji Malick Sy Soda Mbaye Hawo Madina Diallo Audrey Samra aboubacry Sadikh Sow Joseph Matar Mass Ndiaye Ousmane Ndiaga Senghor Lamine Ndiaye Mohamed Ndiaye Serigne Sohibou Gaye Papa Amadou Ndiaye Ophthalmology Department of Abass Ndao Hospital Dakar Senegal Ophthalmology Department of Aristide Le Dantec Hospital Dakar Senegal

Purpose: The aim is to show epidemiological, clinical and etiological characteristics of palpebral wounds. Methodology: This was a retrospective study focusing on patients with an eyelid wound, over a 10-year period from 2012 to 2021. We collected and analyzed the data using Excel. Results: The frequency of eyelid wounds was 0.1%. The average age of our patients was 19.38 years with a range of 1 and 62 years. The sex ratio was 3.7. Eighty-one percent of patients lived in Dakar. Fifty-seven percent (57%) of patients consulted less than 24 hours after the trauma and 7% of patients on D1. The circumstances were brawls (11%), domestic accidents (9%), road accidents (6%), and work accidents (6%). We noted 1 case of dog bite. Thirteen patients presented with post-traumatic decreased visual acuity. Involvement of the isolated upper eyelid was noted in 40% of cases and both eyelids in 15% of cases. Involvement of the lacrimal ducts was noted in 17% of cases, and that of the free edge in 21% of cases. Eyelid wounds were associated with eyeball damage in 21% of cases. Various associated lesions were observed. Ninety-one percent of patients received surgical treatment. Three cases of superinfections, 1 case of conjunctival granuloma and 1 case of phthysis of the eyeball with postoperative retinal detachment were noted. Conclusion: Eyelid sores are relatively common in our context. They require rapid surgical treatment in order to preserve the functional and aesthetic prognosis. .

关键词： Eyelid Wounds Epidemiology Clinic Etiologies

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