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中国解剖学会2019年年会

作者： Jie Wang Bo Chen abiola abdulrahman ayanlaja Dianshuai Gao Department of Neurobiology Xuzhou Medical University

＜正＞To construct a Doublecortin （DCX） knockout vector and to establish a rat C6 glioma cell line stably no-expressing *** observe the effect of DCX gene on the proliferation of C6 glioma cells in rats,and to lay the foundation for the study of the mechanisms involved in glioma dynamics aided by *** observation of green fluorescent protein GFP expression intensity was used to determine the efficiency of lentivirus

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Blunt dopamine transmission due to decreased GDNF in the PFC evokes cognitive impairment in Parkinson’s disease

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Neural Regeneration Research 2023年第5期18卷 1107-1117页

作者： Chuan-Xi Tang Jing Chen Kai-Quan Shao Ye-Hao Liu Xiao-Yu Zhou Cheng-Cheng Ma Meng-Ting Liu Ming-Yu Shi Piniel Alphayo Kambey Wei Wang abiola abdulrahman ayanlaja Yi-Fang Liu Wei Xu Gang Chen Jiao Wu Xue Li Dian-Shuai Gao Department of Neurobiology Xuzhou Key Laboratory of NeurobiologyXuzhou Medical UniversityXuzhouJiangsu ProvinceChina Experinental Teaching Center of Morphology Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Neurology Affiliated Suqian Hospital of Xuzhou Medical UniversitySuqianJiangsu ProvinceChina Department of Rehabilitation Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Neurology Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Medicine Jiangnan UniversityWuxiJiangsu ProvinceChina Department of Nursing Care the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina

Studies have found that the absence of glial cell line-derived neurotrophic factor may be the primary risk factor for Parkinson’s disease. However, there have not been any studies conducted on the potential relationship between glial cell line-derived neurotrophic factor and cognitive performance in Parkinson’s disease. We first performed a retrospective case-control study at the Affiliated Hospital of Xuzhou Medical University between September 2018 and January 2020 and found that a decreased serum level of glial cell line-derived neurotrophic factor was a risk factor for cognitive disorders in patients with Parkinson’s disease. We then established a mouse model of Parkinson’s disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and analyzed the potential relationships among glial cell line-derived neurotrophic factor in the prefrontal cortex, dopamine transmission, and cognitive function. Our results showed that decreased glial cell line-derived neurotrophic factor in the prefrontal cortex weakened dopamine release and transmission by upregulating the presynaptic membrane expression of the dopamine transporter, which led to the loss and primitivization of dendritic spines of pyramidal neurons and cognitive impairment. In addition, magnetic resonance imaging data showed that the long-term lack of glial cell line-derived neurotrophic factor reduced the connectivity between the prefrontal cortex and other brain regions, and exogenous glial cell line-derived neurotrophic factor significantly improved this connectivity. These findings suggested that decreased glial cell line-derived neurotrophic factor in the prefrontal cortex leads to neuroplastic degeneration at the level of synaptic connections and circuits, which results in cognitive impairment in patients with Parkinson’s disease.

关键词： cognitive impairment degree centrality dendritic spine dopamine transmission dopamine transporter glial cell line-derived neurotrophic factor Parkinson’s disease prefrontal cortex synaptic plasticity

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双皮质素过表达载体的构建及其对大鼠脑胶质瘤细胞迁移能力的影响

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江苏医药 2017年第24期43卷 1749-1752,F0002,I0001页

作者：高玥姬广全高殿帅 abiola abdulrahman ayanlaja 徐州医科大学江苏省221004

目的观察过表达双皮质素(DCX)基因对大鼠脑胶质瘤细胞迁移能力的影响。方法采用PCR扩增DCX基因片段,构建DCX慢病毒表达载体,感染293T细胞,荧光显微镜下观察绿色荧光蛋白的表达。筛选稳定表达DCX的大鼠脑胶质瘤C6细胞,将C6细胞分为空载... 详细信息

目的观察过表达双皮质素(DCX)基因对大鼠脑胶质瘤细胞迁移能力的影响。方法采用PCR扩增DCX基因片段,构建DCX慢病毒表达载体,感染293T细胞,荧光显微镜下观察绿色荧光蛋白的表达。筛选稳定表达DCX的大鼠脑胶质瘤C6细胞,将C6细胞分为空载体病毒感染组(GV468组)和过表达DCX病毒感染组(GV468-DCX组),qRT-PCR和Western blot法检测DCX表达。Transwell实验检测过表达DCX对大鼠胶质瘤C6细胞迁移能力的影响。结果成功构建过表达DCX的慢病毒载体,感染C6细胞的效率达到90%。感染C6细胞后,GV468-DCX组DCX mRNA和蛋白表达较GV468组高(P<0.01或P<0.05),GV468-DCX组迁移能力高于GV468组(P<0.05)。结论成功构建DCX慢病毒表达载体,获得稳定表达DCX的大鼠胶质瘤细胞系,过表达DCX基因能促进胶质瘤细胞的迁移能力,为脑胶质瘤的基因治疗提供参考。

关键词：双皮质素脑胶质瘤慢病毒

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