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Matrigel modulates a stem cell phenotype and promotes tumor formation in a mantle cell lymphoma cell line

Stem Cell Discovery 2013年第3期3卷 167-179页

作者： abigail hielscher Timothy McGuire Dennis Weisenburger John Graham Sharp Department of Cell Biology Genetics and Anatomy University of Nebraska Medical Center Omaha USA

Tumors may be maintained by subpopulations of cells possessing stem cell-like properties. We evaluated the stem cell-like and tumor-forming properties of side population (SP) and CD133+/ CD44+ cells in Granta 519, a human mantle cell lymphoma cell line. The in-vitro Cobblestone Area Forming Cell (CAFC) assay, designed to detect stem and progenitor cells, revealed that SP cells contained the greatest proportion of stem cell-like cells. The addition of Matrigel to CAFC assays of SP and non-SP cells both increased their respective stem cell frequencies in comparison to those cultures without Matrigel, and additionally resulted in observed stem cell frequencies which were the same between SP and non-SP cells. Contrary, Matrigel decreased the stem cell frequencies of CD133+/CD44+ or CD133-/CD44- cells. In-vivo assays revealed tumor formation from Matrigel-mixed SP and non-SP cells, and in one instance, occurred with as few as one Matrigel-mixed SP cell. Vehicle-mixed injections of SP and non-SP tumor cells resulted in tumor formation from SP cells only. Tumor formation did not occur from Matrigel nor hyaluronan (cellular substrate for CD44-expressing cells)-mixed populations of CD133+/CD44+ or CD133-/CD44- cells. These data demonstrate that Matrigel modulates a stem cell phenotype and promotes tumor formation from SP and non-SP cells. The tumor micro-environmental niche and tumor cell to micro-environmental interactions may be important future targets for novel chemotherapeutic agents.

关键词： Mantle Cell Lymphoma Side Population Tumor-Initiating Cells Microenvironment

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A DIFFUSION–TRANSPORT HYBRID METHOD FOR ACCELERATING OPTICAL TOMOGRAPHY

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Journal of Innovative Optical Health Sciences 2010年第4期3卷 293-305页

作者： HYUN KEOL KIM ANDREAS H.hielscher Department of Biomedical Engineering Columbia University New YorkNY 10027USA Department of Radiology Columbia University New YorkNY 10032USA Department of Electrical Engineering Columbia University New YorkNY 10027USA

It is well acknowledged that the equation of radiative transfer(ERT)provides an accurate prediction of light propagation in biological tissues,while the diffusion approximation(DA)is of limited accuracy for the transport ***,ERT-based reconstruction codes require much longer computation times as compared to DA-based reconstruction *** introduce here a computationally efficient algorithm,called a diffusion–transport hybrid solver,that makes use of the DA-or low-order ERT-based inverse solution as an initial guess for the full ERT-based reconstruction *** evaluate the performance of this hybrid method,we present extensive studies involving numerical tissue phantoms and experimental *** a result,we show that the hybrid method reduces the reconstruction time by a factor of up to 23,depending on the physical character of the problem.

关键词： Optical tomography reconstruction time radiative transfer equation

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新型喷嘴喷孔改善空气利用率降低碳烟排放

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汽车与新动力 2018年第1期1卷 39-42页

作者： K.hielscher M.Brauer R.Baar 范明强

内燃机形成碳烟的主要因素是局部氧稀薄。传统的柴油机在燃烧过程中,燃烧开始时各个燃油喷束之间的空气尚未被利用。正是出于这个原因,对2种新型喷嘴喷孔进行了试验研究,采用这2种喷嘴喷孔能更好地利用燃油喷束之间的空气,但是却使烧尽... 详细信息

内燃机形成碳烟的主要因素是局部氧稀薄。传统的柴油机在燃烧过程中,燃烧开始时各个燃油喷束之间的空气尚未被利用。正是出于这个原因,对2种新型喷嘴喷孔进行了试验研究,采用这2种喷嘴喷孔能更好地利用燃油喷束之间的空气,但是却使烧尽阶段的空气利用率变差,最终导致较高的颗粒物排放量。为此,法国巴黎理工大学内燃机专业与IAV汽车工程公司合作进行了试验研究。

关键词：柴油机碳烟喷嘴喷孔

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