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Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

Biomedical and Environmental Sciences 2016年第4期29卷 275-285页

作者： Fatma Guesmi Hamida Beghalem Amit K tyagi Manel Ben Ali Ramla Ben Mouhoub Houda Bellamine Ahmed Landoulsi Laboratory of Biochemistry and Molecular Biology Faculty of Sciences of Bizerte Zarzouma Bizerte 7021 Tunisia Cytokine Research Laboratory Department of Experimental Therapeutics The University of Texas M. D. Anderson Cancer Center 1515 Holcombe Boulevard Houston TX 77030 USA Laboratory of Biomonitoring of the Environment (LRO1/ES14) Faculty of Sciences of Bizerte Zarzouma Bizerte 7021 Tunisia Service of Anatomo-Pathology of Menzel Bourguiba Bizerte 7050 Tunisia

Objective We evaluate the effects of Thymus algeriensis （TEO） against hydrogen peroxide （H202） toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats. Methods Rats were treated with low （LD） and high dose （HD） of H202 （0.1 and 1 mmol/L） in the presence or absence of TEO （150 mg/kg）. Results The results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease （P〈0.05）, sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H202 compared to controls. The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity and significant increase （P〈0.05） in MDA concentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2 generated oxidative stress. These results were confirmed by histological architecture examinations. Conclusion H2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.

关键词： Testis Hydrogen peroxide TEO Antioxidants Infertility

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Purification of full-length human Pregnane and Xenobiotic Receptor:polyclonal antibody preparation for immunological characterization

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Cell Research 2005年第10期 39-49页

作者： Mallampati SARADHI,Biji KRISHNA,Gauranga MUKHOPADHYAY,Rakesh K tyagi Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi 110067 India Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi 110067 India Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi 110067 India Special Centre for Molecular Medicine Jawaharlal Nehru University New Delhi 110067 India

Pregnane and Xenobiotic Receptor (PXR; or Steroid and Xenobiotic Receptor, SXR), a new member of the nuclear receptor superfamily, is thought to modulate a network of genes that are involved in xenobiotic metabolism and elimination. To further explore the role of PXR in body’s homeostatic mechanisms, we for the first time, report successful prokary- otic expression and purification of full-length PXR and preparation of polyclonal antibody against the whole protein. The full-length cDNA encoding a 434 amino acids protein was sub-cloned into prokaryotic expression vector, pET-30b and transformed into E. coli BL21(DE3) cells for efficient over expression. The inclusion body fraction, containing the expressed recombinant protein, was purified first by solubilizing in sarcosine extraction buffer and then by affinity column chromatography using Ni-NTA His-Bind matrix. The efficacy of anti-PXR antibody was confirmed by immunocytology, Western blot analysis, EMSA and immunohistochemistry. The antibody obtained was capable of detecting human and mouse PXR with high specificity and sensitivity. Immunofluorescence staining of COS-1 cells transfected with human or mouse PXR showed a clear nuclear localization. Results from immunohistochemistry showed that level of PXR in liver sections is immunologically detectable in the nuclei. Similar to exogenously transfected PXR, Western blot analysis of cell extract from HepG2 and COLO320DM cells revealed a major protein band for endogenous PXR having the expected molecular weight of 50 kDa. Relevance of other immunodetectable bands with reference to PXR isoforms and current testimony are evaluated. Advantages of antibody raised against full-length PXR protein for functional characterization of receptor is discussed and its application for clinical purposes is envisaged.

关键词： Pregnane and Xenobiotic Receptor Steroid and Xenobiotic Receptor prokaryotic expression polyclonal antibody isoforms.

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A hypothesis for treating inflammation and oxidative stress with hydrogen sulfide during age-related macular degeneration

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International Journal of Ophthalmology(English edition) 2018年第5期11卷 881-887页

作者： Akash K George Mahavir Singh Rubens Petit Homme Avisek Majumder Harpal S SANDhu Suresh C tyagi Eye and Vision Science Laboratory Department of PhysiologyUniversity of Louisville School of Medicine Department of Physiology University of Louisville School of Medicine Department of Biochemistry and Molecular Genetics University of Louisville School of Medicine Department of Ophthalmology and Visual Sciences University of Louisville School of Medicine Kentucky Lions Eye Center University of Louisville School of Medicine

Age-related macular degeneration（AMD） is a leading cause of blindness and is becoming a global crisis since affected people will increase to 288 million by ***,age,diabetes,gender,obesity,hypertension,race,hyperopia,iris-color,smoking,sun-light and pyroptosis have varying roles in AMD,but oxidative stress-induced inflammation remains a significant driver of *** is a unique organ as it contains a remarkable oxygengradient that generates reactive oxygen species（ROS） which upregulates inflammatory *** becomes a source of functional and morphological impairments in retinal pigment epithelium（RPE）,endothelial cells and retinal ganglion *** demonstrated that hydrogen sulfide（H2S） acts as a signaling molecule and that it may treat ***,we propose a novel hypothesis that H2S may restore homeostasis in the eyes thereby reducing damage caused by oxidative injury and *** H_2S has been shown to be a powerful antioxidant because of its free-radicals＇ inhibition properties in addition to its beneficial effects in age-relatedconditions,therefore,patients may benefit from H2S salubrious effects not only by minimizing their oxidant and inflammatory injuries to retina but also by lowering retinal glutamate excitotoxicity.

关键词： eye diseases hydrogen sulfide treatment inflammation macula oxidative stress pyroptosis retinal degeneration

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Reliability Analysis of a Powerloom Plant Using Interval Valued Intuitionistic Fuzzy Sets

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Applied Mathematics 2014年第13期5卷 2008-2015页

作者： Sanjay Kumar tyagi General Requirements Department Ibri College of Applied Sciences Ministry of Higher Education Ibri Sultanate of Oman

In this paper, we investigate the reliability analysis of a powerloom plant by using interval valued intuitionistic fuzzy sets (IVIFS). Herein, we modeled a powerloom plant as a gracefully degradable system having two units A(n) and B(m) connected in series. The reliability ofncomponents of unitAandmcomponents of unitBis assumed to be an IVIFS defined over the universe of discourse [0, 1]. Thus, the reliability of the system obtained is an IVIFS that covers the inherited uncertainty in data collection and reliability evaluation of a powerloom plant.

关键词： Reliability Interval Valued Intuitionistic Fuzzy Sets Powerloom Plant Parallel Redundancy Graceful Degradation

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二阶Newton法训练径向基函数神经网络的算法研究

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计算机研究与发展 2015年第7期52卷 1477-1486页

作者：蔡珣陈智 Kanishka tyagi 于宽李子强朱波山东大学计算机科学与技术学院济南250101 德克萨斯大学阿灵顿分校电子工程系美国阿灵顿76019 山东建筑大学材料科学与工程学院济南250101 山东大学材料科学与工程学院济南250061

提出了一种混合加权距离测量(weighted distance measure,weighted DM)参数的构建和训练RBF(radial basis function)神经网络的两步批处理算法.该算法在引进了DM系数参数的基础上,采用Newton法分别对径向基函数的覆盖参数、均值向量参... 详细信息

提出了一种混合加权距离测量(weighted distance measure,weighted DM)参数的构建和训练RBF(radial basis function)神经网络的两步批处理算法.该算法在引进了DM系数参数的基础上,采用Newton法分别对径向基函数的覆盖参数、均值向量参数、加权距离测度系数以及输出权值进行了优化,并在优化过程中利用OLS(orthogonal least squares)法来求解Newton法的方程组.通过实验数据,不仅分析了Newton法优化的各个参数向量对RBF网络训练的影响,而且比较了混合优化加权DM与RLS-RBF(recursive least square RBF neural network)网络训练算法的收敛性和计算成本.所得到的结论表明整合了优化参数的加权DM-RBF网络训练算法收敛速度比RLS-RBF网络训练算法更快,而且具有比LM-RBF(Levenberg-Marquardt RBF)训练算法更小的计算成本,从而说明OLS求解的Newton法对优化RBF网络参数具有重要应用价值.

关键词：径向基函数神经网络 Hessian矩阵 Newton法正交最小二乘法网络参数优化最优学习因子

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Preparation,characterization and thermophysical properties investigation of A70/polyaniline nanocomposite phase change material for medium temperature solar applications

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能源与人工环境(英文) 2021年第3期2卷 271-277页

作者： A.K.Pandey Mathew George Nasrudin Abd Rahim V.V.tyagi Syed Shahabuddin R.Saidur Research Centre for Nano-Materials and Energy Technology(RCNMET) School of Science and TechnologySunway UniversityNo.5Jalan UniversitiBandar SunwayPetaling Jaya47500 Selangor Darul EhsanMalaysia Higher Institution Centre of Excellence(HICoE) UM Power Energy Dedicated Advanced Centre(UMPEDAC)Level 4Wisma R&DUniversity of MalayaJalan Pantai Baharu59990 Kuala LumpurMalaysia Renewable Energy Research Group King Abdulaziz UniversityJeddah 21589Saudi Arabia School of Energy Management Shri Mata Vaishno Devi UniversityKatra 182320(J&K)India Department of Science School of TechnologyPandit Deendayal Petroleum UniversityKnowledge CorridorRaisan VillageGandhinagar 382007GujratIndia

The ever-present demand for energy from various application in industrial and domestic processes has led to the consumption of fossil fuel at a rapid rate with adverse effect due to global *** study focuses on the thermal energy storage aspect intended for medium temperature applications.A novel composite A70 and PANI was prepared and *** study investigates the composites thermophysical and optical *** Scanning Calorimetry and Transient Hot Bridge measured thermal storage capacity and thermal conductivity of the composite,*** heat storage capacity of the composite remained stable within 4%whereas a highest rise of 11.96%in thermal conductivity was *** composites thermal,chemical,and physical stability were analysed from Thermogravimetric Analyser,Fourier Infrared Transform,and Scanning Electron Microscope,*** composites were thermally stable up to a temperature of 250°*** chemical reaction occurred between the nanomaterial and base PCM *** microscopic visuals did not show any considerable change in the microscopic structure of the *** the case of optical properties,the composites showed significant reduction in transmittance of solar spectrum with respect to pure *** maximum decrement in transmission was around~89%compared to *** the composite prepared were thermally stable till 250°C,hence may be utilized for solar thermal and low concentrated photovoltaic application but not limited to these.

关键词： Phase change materials Solar energy Thermal conductivity Latent heat Polyaniline

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Liposome mediated drug delivery for leukocyte adhesion deficieny I （LAD I）： Targeting the mutated gene ITGB2 and expression of CD18 protein

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Frontiers in Biology 2014年第1期9卷 1-4页

作者： C. Subathra DEVI Kritika KEDARINATH Payal CHOUDHARY Vishakha tyagi Mohanasrinivasan. V School of Biosciences and Technology VIT University Vellore 632014 Tamil Nadu India

Leukocyte adhesion deficiency （LAD） I is a disorder caused due to mutations in a gene （ITGB2） located on chromosome 21 and encodes the β2 subunit of the leukocyte integrin molecules. This leads to defects in the adhesion of leukocytes on endothelial cells which further leads to recurrent microbial infections due to a decrease in the immune response. Base Excision Repair Mechanism （BER） is instrumental in repairing damaged DNA by removing mutated/ damaged bases. We have proposed a hypothesis for the treatment of LAD I by making use of the proteins/enzyme complexes responsible for base excision repair mechanism be introduced into the leukocytes via liposomes. This will target the mutated gene in the leukocytes （mostly neutrophils） and DNA repair will occur. The liposomes can be introduced into the patients via intravenous methods.

关键词： CDI8 β-integrin LAD I liposome BER ITGB2

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不同冲击冷却结构对旋转效应的敏感性

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工程热物理学报 2016年第1期37卷 37-41页

作者：杨力 Kartikeya tyagi 任静 Srinath Ekkad 蒋洪德清华大学热能工程系北京100084 弗吉尼亚理工学院

本文针对多排孔冲击和通道之间倾斜冲击结构进行了旋转条件下数值模拟研究,通过流场和传热分析了不同结构和不同部位对旋转科氏力和离心力的敏感性。冲击射流动量较大,旋转数小,且旋转二次流发展空间不足,导致多排孔冲击结构对旋转的敏... 详细信息

本文针对多排孔冲击和通道之间倾斜冲击结构进行了旋转条件下数值模拟研究,通过流场和传热分析了不同结构和不同部位对旋转科氏力和离心力的敏感性。冲击射流动量较大,旋转数小,且旋转二次流发展空间不足,导致多排孔冲击结构对旋转的敏感性降低。通道之间双排倾斜冲击结构对旋转的影响比多排孔冲击结构敏感,主要体现在下游前缘的冲击靶面上。根据旋转数大小采用非对称的射流结构可以优化传热分布。

关键词：旋转冲击冷却科氏力

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Significant Factors for Reliability Estimation of Component Based Software Systems

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Journal of Software Engineering and Applications 2014年第11期7卷 934-942页

作者： Kirti tyagi Arun Sharma Ajay Kumar Garg Engineering College Ghaziabad India Department of Information Technology Indira Gandhi Delhi Technical University for Women (IGDTUW)Kashmere Gate Delhi

Software reliability is defined as the probability of the failure-free operation of a software system for a specified period of time in a specified environment. Traditional approaches for software reliability analysis are black box approaches. These approaches use the software as a whole. At present, main emphasis of software is on reuse, hence component based software applications came into existence. Black box models are not appropriate for these applications. This paper introduces some significant factors for reliability estimation of Component Based Software Applications. Reliability of Component Based Software Application depends upon these factors. This paper?also gives the definition of factors and explains its relation with reliability of software application.

关键词： Failure Rate Reliability Component Based Systems Flexibility Operational Profile

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Caring for the elderly

Caring for the elderly

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作者： (editor.)Paltasingh Tattwamasi (editor.)tyagi Renu

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