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Quantitation of tadalafil in human plasma using a sensitive and rapid LC-MS/MS method for a bioequivalence study

Journal of Pharmaceutical Analysis 2018年第4期8卷 271-276页

作者： abhaysingh bhadoriya Bhavesh Dasandi Dharmesh Parmar Priyanka A. Shah Pranav S. Shrivastav Bioanalytical Department Synchron Research Services Pvt. Ltd. Department of Chemistry School of Sciences Gujarat University

A highly sensitive liquid chromatography-tandem mass spectrometry（LC-MS/MS） method was developed for the determination of tadalafil（TAD） in human plasma. TAD and its deuterated internal standard（IS）, tadalafil-d3, were extracted from 200 mL plasma using Phenomenex Strata-X-C 33 m extraction cartridges. Chromatographic analysis was carried out on Synergi Hydro-RP C18（100 mm × 4.6 mm, 4 mm）column with a mobile phase consisting of methanol and 10 m M ammonium formate, p H 4.0（90：10, v/v）,delivered at a flow rate of 0.9 m L/min. Quantitation of the protonated analyte was done on a triple quadrupole mass spectrometer using multiple reaction monitoring via electrospray ionization. The precursor to product ions transitions monitored for TAD and TAD-d3 were m/z 390.3-268.2 and m/z393.1-271.2, respectively. The calibration curve was linear over the concentration range of 0.50–500 ng/m L with correlation coefficient, r2 Z 0.9994. Acceptable intra-batch and inter-batch precision（r3.7%） and accuracy（97.8% to 104.1%） were obtained at five concentration levels. The recovery of TAD from spiked plasma was highly precise and quantitative（98.95% to 100.61%）. Further, the effect of endogenous matrix components was minimal. TAD was found to be stable under different storage conditions in human plasma and also in whole blood samples. The validated method was successfully used to determine TAD plasma concentration in a bioequivalence study with 20 mg TAD tablets in 24 healthy volunteers. Method performance was evaluated by reanalyzing 115 study samples.

关键词： Tadalafil Tadalafil-d3 LC-MS/MS Sensitive Bioequivalence study Human plasma

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Sensitive and rapid determination of amantadine without derivatization in human plasma by LC–MS/MS for a bioequivalence study

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Journal of Pharmaceutical Analysis 2018年第3期8卷 202-207页

作者： abhaysingh bhadoriya Shivprakash Rathnam Bhavesh DasANDi Dharmesh Parmar Mallika Sanyal Pranav S.Shrivastav Bioanalytical Department Synchron Research Services Pvt.Ltd Department of Chemistry St.Xavier's College Department of Chemistry School of Sciences Gujarat University

A highly sensitive, rapid and rugged liquid chromatography-tandem mass spectrometw （LC-ESI-MS/MS） method was developed for reliable estimation of amantadine （AMD）, an antiviral drug in human plasma. The analyte and internal standard （IS）, amantadine-d6 （AMD-d6）, were extracted from 200μL plasma by solid phase extraction on Phenomenex Strata-X-C 33 ~t cartridges. Chromatography was performed on Synergi^TM Hydro-RP C18 （150 mm×4.6 mm, 4 μm） analytical column using a mixture of acetonitrile and 10 mM ammonium formate, pH 3.0 （80：20, v/v） as the mobile phase. Detection and quantitation was done by multiple reaction monitoring in the positive ionization mode for AMD （m/z 152.1→ 135.1 ） and IS （m/z 158.0 → 141.1） on a triple quadrupole mass spectrometer. The assay was linear in the concentration range of 0.50-500 ng/mL with correlation coefficient （r^2）〉 0.9969. The limit of detection of the method was 0.18 ng/mL The intra-batch and inter-batch precisions were 〈 5.42% and the accuracy varied from 98.47% to 105.72%. The extraction recovery of amantadine was precise and quantitative in the range of 97.89%-100.28%. IS-normalized matrix factors for amantadine varied from 0.981 to 1.012. The stability of AMD in whole blood and plasma was evaluated under different conditions. The developed method was successfully applied for a bioequivalence study with 100 mg of AMD in 32 healthy volunteers. The reproducibility of the assay was determined by reanalysis of 134 subject samples.

关键词： Amantadine Amantadine-d6 LC-ESI-MS/MS Sensitive Bioequivalence study Human plasma

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A review on re-emerging bacteriophage therapy in the era of XDR

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生物细胞(英文) 2023年第9期47卷 1915-1930页

作者： POOJA bhadoriya RICHA SHARMA REKHA MEHROTRA SIMRAN KAUR ISHA SRIVASTAVA MUKUL JAIN PRASHANT KAUSHIK Department of Microbiology Shaheed Rajguru College of Applied Sciences for WomenUniversity of DelhiVasundhara EnclaveNew Delhi110096India Lab-209 Cell&Developmental biology labCenter of Research for DevelopmentParul UniversityVadodara391760GujaratIndia Instituto de Conservacióny Mejora de la Agrodiversidad Valenciana Universitat Politècnica de ValènciaValencia46022Spain

In the present medicine world antibiotic resistance is one of the key threats to universal health *** continue to work hard to combat this global health *** therapy,an age-old practice during the early twentieth century,was outshined by the discovery of *** the advent of widespread antibiotic resistance,phage therapy has again redeemed itself as a potential alternative owing to its adeptness to target bacteria *** side effects,the ability to migrate to different body organs,a distinct mode of action,and proliferation at the infection site,make phages a profitable candidate to replace conventional *** progressive outcome of numerous in vitro studies and case reports has validated the clinical efficacy of phage *** bright perspective of using phages to treat bacterial infections has fueled enormous medical research to exploit their potential as *** gaps in the information about phages and the lack of consent for clinical trials is major hurdle for consideration of phage *** phage therapy as a reality in medicine requires a coordinated effort from different *** this review,we aim to emphasize the importance of phage therapy in modern *** review explains their historical journey,basic phage biology,cross-talk with the host immunity,obstacles with phage therapy,and their possible *** data on the various significant clinical trials of phage therapy has been *** evaluated the efficacy of antibiotics and phage therapy in part and in combination,along with recent progress and future perspectives of phage therapy.

关键词： Bacteriophages Antibiotic resistance Phage biology Phage therapy Phage therapy clinical trials Phage therapy in India

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Intelligent Diagnostic Method for Ageing Analysis of Transformer

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Energy and Power Engineering 2012年第2期4卷 53-58页

作者： Anil Kumar Kori Arvind Kumar Sharma Ashok Kumar Singh bhadoriya Department of Electrical Engineering Jabalpur Engineering College Jabalpur Indi Department of Electrical Engineering Jabalpur Engineering College Jabalpur India Rajiv Gandhi Technical University Bhopal India

The fuzzy method is proposed for ageing analysis of transformer. The fuzzy controller is used for various input and one output, strictly depends on the number of membership function and there rule base and the type of the defuzzification method. The ageing of transformers is influenced by short term and long term over loads, number and intensity of short circuits, incidence of lightning, and internal faults. The recent development of various techniques for detecting the incipient fault conditions have been improved to some extent;the life expectancy of transformers by resorting to corrective actions in time. The ageing behavior is likely to be different for different types of transformers. The life span of the transformer, thus depends initially on the design and quality of manufacture and later on service conditions and maintenance standard, these factors vary considerably and affect the useful span of service life which therefore needs to be taken into account for residual life assessment. During the natural ageing of transformers, the insulation of winding deteriorates. Cellulose insulation degrades due to heating or electrical breakdown which is dissolved in oil. Hence, the chemical analysis of the Transformer oil gives evidence of changes that are taking place in the winding insulation during operation. Deterioration in transformer cellulose decreases both its electrical and mechanical strength. In this paper a novel fuzzy based algorithm has been implemented on three samples of power transformer oil.

关键词： Transformer Oil Paper Insulation Electrical Breakdown Ageing Analysis Fuzzy Logic Controller

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Lecithin organogel: A unique micellar system for the delivery of bioactive agents in the treatment of skin aging

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Acta Pharmaceutica Sinica B 2012年第1期2卷 8-15页

作者： Sushil Raut Santosh Singh bhadoriya Vaibhav Uplanchiwar Vijay Mishra Avinash Gahane Sunil Kumar Jain Department of Pharmacy Adina Institute of Pharmaceutical SciencesSagar 470002India Department of Pharmaceutical Sciences Dr.H.S.Gour UniversitySagar 470003India

Skin aging is an unavoidable aspect of human *** skin aging can result from poor care,environmental pollutants,and ultraviolet radiation ***,lines,spots,uneven skin tone,and pigmentation are often indicators of skin *** cannot avoid aging but cosmetics and pharmaceutical approaches can minimize and delay the *** applications of biocompatible and biodegradable vehicles have been explored for delivering anti-aging *** organogel(LO)is an effective vehicle for topical delivery of many bioactive agents used in aging *** is cell component isolated from soya beans or eggs and purified to show excellent gelation in non-polar solvents when combined with *** can form a heat-stable,resistant to microbial growth,visco-elastic,optically transparent,and non-birefringent micellar *** serves as an organic medium to enhance dermal permeation of poorly permeable drugs by effectively partitioning into the *** ability to dissolve in hydrophilic as well as in lipophilic drugs makes it a dynamic vehicle,which can be explored as a carrier for anti-aging agents.

关键词： Skin aging Topical Lecithin Biocompatible Organogel

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Intelligent Diagnostic Method for Ageing Analysis of Transformer

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Energy and Power Engineering（能源与动力工程） 2012年第2期

作者： Anil Kumar Kori Arvind Kumar Sharma Ashok Kumar Singh bhadoriya

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