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3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities

Journal of Materials Science & Technology 2021年第4期63卷 9-17页

作者： Tzu-Cheng Sung Chao-Wen heish henry hsin-Chung Lee Jhe-Yu hsu Chun-Ko Wang Jia-hua Wang Yu-Ru Zhu Shih-hsi Jen Shih-Tien hsu abdurahman hhirad Abdullah AAlarfaj Akon higuchi School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityNo.270Xueyuan RoadWenzhouZhejiang325027China Department of Chemical and Materials Engineering National Central UniversityTaoyuan 32001Taiwan Department of Surgery Hsinchu Cathay General HospitalHsinchu 30060China Graduate Institute of Translational and Interdisciplinary Medicine National Central UniversityTaoyuan 32001Taiwan Department of Obstetrics and Gynecology Landseed HospitalTaoyuan 32405Taiwan Department of Internal Medicine Landseed HospitalTaoyuan 32405Taiwan Department of Botany and Microbiology King Saud UniversityRiyadh 11451Saudi Arabia Wenzhou Institute University of Chinse Academy of ScienceWenzhou 325001China Center for Emergent Matter Science Riken2-1 HirosawaWakoSaitama 351-0198Japan

human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and ***,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension *** this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing *** hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process ***,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to ***,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D *** reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing *** study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in *** discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility.

关键词： culture alone stem

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Erratum to“3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities”[Journal of Materials Science&Technology 63(2021)9-17]

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Journal of Materials Science & Technology 2022年第16期111卷 307-307页

作者： Tzu-Cheng Sung Chao-Wen heish henry hsin-Chung Lee Jhe-Yu hsu Chun-Ko Wang Jia-hua Wang Yu-Ru Zhu Shih-his Jen Shih-Tien hsu abdurahman h.hirad Abdullah A.Alarfaj a School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityNo.270Xueyuan RoadWenzhouZhejiang 325027China Department of Chemical and Materials Engineering National Central UniversityTaoyuanTaiwan 32001China Department of Surgery Hsinchu Cathay General Hospital30060China Graduate Institute of Translational and Interdisciplinary Medicine National Central UniversityTaoyuanTaiwan 32001China Department of Obstetrics and Gynecology Landseed HospitalTaoyuanTaiwan 32405China Department of Internal Medicine Landseed HospitalTaoyuanTaiwan 32405China Department of Botany and Microbiology King Saud UniversityRiyadh 11451Saudi Arabia Wenzhou Institute University of Chinese Academy of ScienceWenzhou 325001China

The affiliations of several authors were modified during the proofing stage at the request of corresponding author Akon higuchi,without notifying the involved authors and the Editorial *** communication with all autho... 详细信息

关键词： parties agreed request

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GTF2h4 regulates partial EndMT via NF-κB activation through NCOA3 phosphorylation in ischemic diseases

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The Innovation 2024年第2期5卷 27-41页

作者： Zheyan Fang Gang Zhao Shuang Zhao Xueting Yu Runyang Feng You-en Zhang haomin Li Lei huang Zhenyang Guo Zhentao Zhang Mukaddas abdurahman hangnan hong Peng Li Bing Wu Jinhang Zhu Xin Zhong Dong huang hao Lu Xin Zhao Zhaoyang Chen Wenbin Zhang Junjie Guo hongchao Zheng Yue he Shengying Qin haojie Lu Yun Zhao Xiangdong Wang Junbo Ge hua Li Department of Cardiology Zhongshan HospitalFudan UniversityShanghai Institute of Cardiovascular DiseasesShanghai 200032China Department of Cardiology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Medical Examination Shanghai Xuhui District Central HospitalShanghai 200031China Department of Cardiology and Institute of Clinical Medicine Renmin HospitalHubei University of MedicineShiyan 442000China Clinical Data Center Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Molecular Celland Cancer BiologyUniversity of Massachusetts Chan Medical SchoolWorcesterMA 01605USA Bio-X Institute Key Laboratory for The Genetics of Developmental and Neuropsychiatric DisordersShanghai Jiao Tong UniversityShanghai 200030China Department of Cardiology Heart Center of Fujian ProvinceFujian Medical University Union HospitalFuzhou 350001China Department of Cardiology Sir Run Run Shaw Hospitalaffiliated with Zhejiang University School of MedicineHangzhou 310020China Department of Cardiology The Affiliated Hospital of Qingdao UniversityQingdao 266003China Department of Cardiology Shanghai Xuhui District Central HospitalShanghai 200031China Department of Cardiology Shanghai Eighth People’s HospitalShanghai 200235China Department of Chemistry and Institutes of Biomedical Sciences Fudan UniversityShanghai 200032China State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology Shanghai Tech University100 Haike RoadShanghai 201210China Key Laboratory of Systems health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Department of Pulmonary and Critical Care Medicine Zhongshan Hospital

Partial endothelial-to-mesenchymal transition(EndMT)is an intermediate phenotype observed in endothelial cells(ECs)undergoing a transition toward a mesenchymal state to support neovascularization during(patho)physiological ***,we investigated the occurrence of partial EndMT in ECs under hypoxic/ischemic conditions and identified general transcription factor IIh subunit 4(GTF2h4)as a positive regulator of this *** addition,we discovered that GTF2h4 collaborates with its target protein excision repair cross-complementation group 3(ERCC3)to co-regulate partial ***,by using phosphorylation proteomics and site-directed mutagenesis,we demonstrated that GTF2h4 was involved in the phosphorylation of receptor coactivator 3(NCOA3)at serine 1330,which promoted the interaction between NCOA3 and p65,resulting in the transcriptional activation of NF-κB and the NF-kB/Snail signaling axis during partial *** vivo experiments confirmed that GTF2h4 significantly promoted partial EndMT and angiogenesis after ischemic ***,our findings reveal that targeting GTF2h4 is promising for tissue repair and offers potential opportunities for treating hypoxic/ischemic diseases.

关键词： ischemic activation GTF

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Bi_(2)Ga_(3.985)O_(9):1.5%Fe^(3+),Eu^(3+)长余辉材料的制备及光学性能研究

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光学学报 2024年第2期44卷 236-245页

作者：茹鲜古丽·艾外力孙艳美库尔班江·努尔麦提赵天骐热娜古丽·阿不都热合曼尹学博喀什大学化学与环境科学学院新疆特色药食用植物资源化学实验室新疆喀什844000 上海工程技术大学化学化工学院前沿医学技术研究院上海201620

采用共沉淀法制备了Bi_(2-x)Ga_(3.985)O_(9):1.5%Fe^(3+),x Eu^(3+)(BGO:1.5%Fe^(3+),x Eu^(3+),x=0~2%)长余辉纳米粒子(PLNP),详细研究了Eu^(3+)掺杂浓度及煅烧温度对BGO:1.5%Fe^(3+)PLNP晶体结构和光学性质的影响。结果显示,最佳的PLNP组成为Bi1.99Ga3.985O9:1.5%Fe^(3+),1%Eu^(3+),属于莫来石晶体结构,发射峰处于798 nm,在900℃煅烧1 h时,可获得高纯度的BGO:1.5%Fe^(3+),1%Eu^(3+)PLNP,其平均电子陷阱能级深度为0.676 eV。与Fe^(3+)单掺杂BGO:1.5%Fe^(3+)PLNP相比,Eu^(3+)共掺杂BGO:1.5%Fe^(3+),1%Eu^(3+)PLNP后,荧光寿命(τ_(av))从13.77 s增大至15.56 s,余辉发光时间从3 h延长至8 h以上。由于共掺杂BGO:1.5%Fe^(3+),1%Eu^(3+)PLNP中存在从Eu^(3+)到Fe^(3+)的能量传递,共掺杂PLNP的余辉强度增大,发光时间延长。制备了长波长发射、具有余辉发光性能的BGO:1.5%Fe^(3+),1%Eu^(3+)PLNP,该材料在生物成像、疾病检测及生物传感等领域具有巨大的应用潜力。

关键词：材料近红外发光长余辉纳米粒子共掺杂镓酸铋

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Comparative study of the characterisation and extraction techniques of polyphenolic compounds from Acacia seyal gum

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Food Quality and Safety 2022年第1期6卷 24-33页

作者： Ahmed A.M.Elnour Mohamed E.S.Mirghani Nassereldeen A.Kabbashi Khalid hamid Musa Fahimeh Shahabipour Nureddin Ashammakhi Nour hamid abdurahman International Institute for halal Research and Training International Islamic University MalaysiaKuala LumpurMalaysia Bioenvironmental Engineering Research Centre Biotechnology Engineering DepartmentKuliyah of EngineeringInternational Islamic University MalaysiaKuala LumpurMalaysia Institute of Gum Arabic&Desertification Studies University of KordofanElobiedSudan Department of Food Science and human Nutrition College of Agriculture and Veterinary MedicineQassim UniversityBuraydahKingdom of SaudiArabia Skin Research Centre Shahid Beheshti University of Medical SciencesTehranIran Department of Bioengineering Henry Samueli School of EngineeringUniversity of CaliforniaLos AngelesCAUSA Department of Biomedical Engineering College of EngineeringMichigan State UniversityEast LansingMIUSA Centre of Excellence for Advanced Research in Fluid Flow University Malaysia PahangGambangMalaysia

Background Acacia seyal gum(ASG)is an abundant source of natural polyphenolic compounds(NPPCs)and antioxidant activity with numerous benefits and is often used in cancer *** type of extraction technique can significantly impact the yield and isolation of NPPCs from *** traditional use of maceration extraction reportedly yields fewer *** This study investigated five extraction techniques for NPPCs and ASG antioxidant activity,namely:homogenisation,shaking,ultrasonication,magneticstirring,*** and methods The evaluation of the antioxidant activity(AoA)of the extracted NPPCs from ASG used five assays,namely:total flavonoids content,Folin-Ciocalteu index,2,2-diphenyl-1-picrylhydrazyl radical scavenging activity,ferric reducing antioxidant power,and cupric *** To minimise the data set dimensionality requires principal component *** ultrasonic and maceration techniques were the best techniques to extract NPPCs and examine the AoA of ASG,with a high correlation between the NPPCs and ***,the maceration process was slow(12 h)compared to ultrasonication(1 h).Slow extraction can result in a decline of the NPPCs due to polyphenol oxidase-enzyme and impact *** These findings provide an essential guide for the choice of extraction techniques for the effective extraction of NPPCs from ASG and other plant materials.

关键词： Polyphenolic extraction gum Arabic comparison of techniques principal component analysis ultrasonication

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榅桲子中三萜类成分及体外生物活性研究

榅桲子中三萜类成分及体外生物活性研究

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作者：阿卜杜热合曼·努如拉（abdurahman Nurulla）新疆医科大学

学位级别：硕士

目的:结合光谱和色谱法分析方法,对榅桲子三萜类成分进行定性和定量分析,并测定榅桲子三萜类成分对DPPh·自由基清除能力、对α-葡萄糖苷酶的抑制以及对环氧化酶2的抑制作用,初步评价榅桲子三萜类成分体外生物活性。方法:1.用甲醇超声... 详细信息

目的:结合光谱和色谱法分析方法,对榅桲子三萜类成分进行定性和定量分析,并测定榅桲子三萜类成分对DPPh·自由基清除能力、对α-葡萄糖苷酶的抑制以及对环氧化酶2的抑制作用,初步评价榅桲子三萜类成分体外生物活性。方法:1.用甲醇超声提取榅桲子中三萜类成分,考察并确定最佳薄层色谱(TLC)条件,鉴别榅桲子中齐墩果酸、熊果酸和委陵菜酸。2.基于单因素试验,利用响应面法进行优化并确定最佳提取工艺。3.以榅桲子总三萜含量为指标,考察固定相、上样条件和洗脱条件,确定最佳纯化工艺条件。4.传统方法提取榅桲子,用多种色谱技术分离富集榅桲子三萜类成分。5.应用hPLC法,用Kromasil 100-5-C色谱柱、以甲醇-0.2%磷酸(70:30)为流动相、流速为1.0 mL/min、柱温30℃、进样体积为10μL、检测波长为210 nm下测定榅桲子中蔷薇酸和委陵菜酸的含量。6.测定榅桲子三萜类成分对DPPh·自由基清除能力、对α-葡萄糖苷酶的抑制作用以及对环氧化酶2的抑制作用,初步探讨榅桲子三萜类成分的体外生物活性。结果:1.用最佳TLC条件即:高效硅胶hSGF板、点样量为4.0μL、1%碘-二氯甲烷溶液预处理、展开剂为甲苯-乙酸乙酯-冰醋酸(14:4:0.5)、显色剂为10%硫酸乙醇溶液来分鉴别榅桲子中齐墩果酸、熊果酸和委陵菜酸。2.最佳提取工艺条件为:按1:20(g/mL)料液比、以无水乙醇为溶剂在41℃下提取40 min得到的榅桲子总三萜的含量为72.32 mg/g。3.最佳纯化工艺为:用D101型大孔吸附树脂、按树脂径高比为1:4、以1 mL/min流速下上样50 mL ph为6、浓度为2 mg/mL的榅桲子总三萜提取液,再用ph为7的80%乙醇以1 mL/min的流速下进行洗脱,得到的总三萜含量从7.36%提高到48.00%。4.硅胶、Sephadex Lh-20等色谱法,从榅桲子提取物中分富集三萜类成分,再用Sephadex Lh-20色谱进一步分离得到一个单体化合物,用h-NMR、C-NMR和质谱确定为熊果酸。5.用hPLC法分离并蔷薇酸对照品保留时间比较确定并含量测定,榅桲子中蔷薇酸和委陵菜酸的含量分别为734.03μg/g和294.77μg/g。6.当榅桲子三萜类成分浓度达到3.0 mg/mL,对DPPh·自由基清除率高达87.48%;当榅桲子三萜类成分和阿卡波糖浓度达到1280μg/mL,两者对α-葡萄糖苷酶的抑制率值分别为58.07%和41.33%;当榅桲子三萜类成分浓度达到100μg/mL时,对COX-2的抑制率为80%。结论:通过优化TLC条件,实现榅桲子中齐墩果酸、熊果酸和委陵菜酸TLC同时鉴别;确定了榅桲子总三萜的最佳提取和纯化工艺;通过多种色谱法分离并利用光谱法鉴定熊果酸和蔷薇酸;hPLC法测定榅桲子中蔷薇酸和委陵菜酸的含量;初步评价榅桲子三萜类成分可能有一定的抗氧化、降血糖和抗炎活性。

关键词：榅桲子总三萜含量测定生物活性

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Zn_(1+x)Ga_(2-0.01-y)Ge_(x)O_(3x+4)∶0.01Cr,yBi长余辉纳米粒子的制备及其光学性质研究

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激光与光电子学进展 2021年第21期58卷 242-249页

作者：热娜古丽·阿不都热合曼杨通胜刘卫国燕音喀什大学化学与环境科学学院新疆特色药食用植物资源化学实验室新疆喀什844006

采用水热合成及后续煅烧法制备了Zn_(1+x)Ga_(2-0.01-y)Ge_(x)O_(3x+4)∶0.01Cr,yBi(x=1~4,y=0~0.04)长余辉纳米粒子(PLNPs)。通过优化反应条件、Zn和Ge组成比例及Cr/Bi共掺杂比例,提高了材料的余辉发光性能。考察了煅烧温度和Cr/Bi共... 详细信息

采用水热合成及后续煅烧法制备了Zn_(1+x)Ga_(2-0.01-y)Ge_(x)O_(3x+4)∶0.01Cr,yBi(x=1~4,y=0~0.04)长余辉纳米粒子(PLNPs)。通过优化反应条件、Zn和Ge组成比例及Cr/Bi共掺杂比例,提高了材料的余辉发光性能。考察了煅烧温度和Cr/Bi共掺杂比例对PLNPs余辉发光性能和物相的影响,结果表明:当水热反应温度为180℃,水热反应时间为24 h,x取2,y取0.02时,样品的余辉发光性质最好。所制备的PLNPs的最佳组成为Zn_(3)Ga_(1.97)Ge_(2)O_(10)∶0.01Cr,0.02Bi,其具有尖晶石结构,余辉时间大于15 d,具有良好的长余辉发光性能。

关键词：材料长余辉纳米粒子纳米材料镓锗酸锌体系 Cr/Bi共掺杂

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如何提高传染性肺结核病人的发现率?——埃塞俄比亚索马里地区的成功经验

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国际结核病与肺部疾病杂志 2003年第2期 78-80页

作者： M-L.Lambert h.Sugulle D.Seyoum S.abdurahman A.Abdinasir M.Frieden F.Matthys P.Van der Stuyft 马玙 Department of Public health Institute For Tropical MedicineAntwerpBelgium Medecins Sans Frontieres Belgium in Ethiopia Jijiga Somali National Regional State health Bureau JijigaEthiopia Medecins Sans Frontieres Belgium in Ethiopia Jijiga Medecins Sans Frontieres Belgium Brussels Belgium Department of Public health Institute For Tropical Medicine AntwerpBelgium

1999年初,埃塞俄比亚索马里地区发现的肺结核(PTB)病例中48％者为痰涂片阳性。于是提出实验室水平上的质量强化措施,并对痰涂片阴性的肺结核诊断进行复核。临床医生虽然了解国家结核病规划关于肺结核诊断的基本原则,但并没有完全遵循这... 详细信息

1999年初,埃塞俄比亚索马里地区发现的肺结核(PTB)病例中48％者为痰涂片阳性。于是提出实验室水平上的质量强化措施,并对痰涂片阴性的肺结核诊断进行复核。临床医生虽然了解国家结核病规划关于肺结核诊断的基本原则,但并没有完全遵循这些原则,部分是因为考虑到病人的费用问题。通过上述非威胁性方式对临床医生提出挑战,发挥其临床实践当中的积极主动性获得了巨大成功,涂阳肺结核比例增至65％。需要开展研究以评估这些广泛推行的涂阴肺结核诊断指南的可行性。

关键词：结核病诊断控制实用指南

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光波导传感元件在检测酸性气体中的应用

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化学研究与应用 2012年第3期24卷 395-399页

作者：热娜古丽·阿不都热合曼阿布力孜·伊米提喀什师范学院化学与环境科学系新疆喀什844007 新疆大学化学化工学院新疆乌鲁木齐830046

采用旋转甩涂法将甲基紫传感薄膜固定在特制的锡(Sn2+,Sn4+)掺杂玻璃光波导(Sn doped glass slide)表面,研究了该传感薄膜与hCl、h2S、以及SO2作用前后的可见吸收光谱的变化,并在此基础上研制了玻璃光波导酸性气体(hCl、h2S、SO2)传感... 详细信息

采用旋转甩涂法将甲基紫传感薄膜固定在特制的锡(Sn2+,Sn4+)掺杂玻璃光波导(Sn doped glass slide)表面,研究了该传感薄膜与hCl、h2S、以及SO2作用前后的可见吸收光谱的变化,并在此基础上研制了玻璃光波导酸性气体(hCl、h2S、SO2)传感元件。传感薄膜与酸性气体作用时,薄膜的颜色发生变化,从而降低薄膜对倏逝波的吸收,使传感器的输出光强度(信号)增强。本文采用流动注射法对酸性气体进行了检测。实验结果表明,在室温下,该传感元件对硫化氢气体具有明显的响应,而对相同浓度的其他酸性气体的响应相对较小,对浓度在6×10-4～2.5×10-5(V/V)的硫化氢气体具有良好的线性响应(R=0.9979,n=4),相对标准偏差(RSD)为±3.5%,具有响应快、可逆性和重复性好、容易制备、可以在室温下工作等特点。

关键词：传感器光波导酸性气体甲基紫

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断层几何学特征及对浊积岩的控制作用

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西南石油大学学报（自然科学版） 2008年第2期30卷 35-37页

作者：王志坤钟建华艾合买提江.阿布都热合曼中国石油大学地球资源与信息学院山东东营257061

为解释断层下降盘浊积砂体横向不均一的控制机理,从河125断层几何特征入手,剖析了断层形态与浊积砂体的关系,发现弧形断裂平行于断层走向剖面上为断槽、断脊组合;在垂直于断层走向剖面上断层可分为竖滑段、倾滑段和平滑段三段。生长指... 详细信息

为解释断层下降盘浊积砂体横向不均一的控制机理,从河125断层几何特征入手,剖析了断层形态与浊积砂体的关系,发现弧形断裂平行于断层走向剖面上为断槽、断脊组合;在垂直于断层走向剖面上断层可分为竖滑段、倾滑段和平滑段三段。生长指数规律为弧中大于弧翼;h/L指数为弧中小于弧翼。断裂的几何学特征与动力学、运动学特征具有相关性,断裂不同位置几何学特征的不同反映了断裂动力学、运动学特征的差异。断层对浊积岩具有重要的控制作用:弧中对应浊积扇最发育区,弧翼为不同浊积扇的分界线;断槽为浊积砂体的补给通道;断脊为扇体不同补给区的分水岭,形成了典型的弧扇对应现象。

关键词：弧扇对应弧形断裂浊积扇几何特征

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