检索结果-南通市图书馆

奇果菌素调控miR-646/ADAM17通路对白血病细胞增殖、凋亡的影响及机制

在线全文

学校读者我要写书评

暂无评论

河北医药 2020年第24期42卷 3702-3707页

作者：努尔阿米娜·依明尼亚孜帕力旦·艾沙阿依姆妮萨·阿卜杜热合曼新疆维吾尔自治区喀什地区第一人民医院血液科 844000

目的探讨奇果菌素(Gri)对白血病细胞增殖、凋亡的影响及其作用机制。方法体外培养白血病K562细胞,经不同浓度的Gri处理细胞后,采用甲基噻唑基四唑(MTT)法测定Gri对细胞增殖的抑制作用;流式细胞术检测细胞凋亡;实时荧光定量PCR(qRT-PCR)... 详细信息

目的探讨奇果菌素(Gri)对白血病细胞增殖、凋亡的影响及其作用机制。方法体外培养白血病K562细胞,经不同浓度的Gri处理细胞后,采用甲基噻唑基四唑(MTT)法测定Gri对细胞增殖的抑制作用;流式细胞术检测细胞凋亡;实时荧光定量PCR(qRT-PCR)与蛋白免疫印迹法(Western blot)检测Gri对微小RNA-646(miR-646)与解聚素-金属蛋白酶17(ADAM17)表达的影响;双荧光素酶报告基因检测miR-646与ADAM17的靶向作用关系;观察miR-646过表达或干扰ADAM17表达对细胞增殖及凋亡的影响,以及抑制miR-646表达后细胞抑制率及凋亡率的变化;Western blot检测细胞周期蛋白1(CyclinD1)、B淋巴细胞瘤-2(Bcl-2)、p21、B淋巴细胞瘤-2相关蛋白(Bax)蛋白表达。结果Gri呈剂量依赖性抑制K562细胞增殖(PiR-646、p21、Bax表达(PinD1、Bcl-2表达(PiR-646可负性调控ADAM17的表达;miR-646过表达或干扰ADAM17表达后可显著抑制细胞增殖,促进细胞凋亡,抑制miR-646表达可逆转Gri对细胞增殖、凋亡的作用。结论奇果菌素可通过调控miR-646/ADAM17通路抑制细胞增殖及促进其凋亡从而发挥抗白血病作用。

关键词：奇果菌素白血病 miR-646 ADAM17 增殖凋亡

3D打印干实验室模型在腹腔镜抗反流手术训练中的应用

在线全文

学校读者我要写书评

暂无评论

中华实验外科杂志 2020年第4期37卷 611-614页

作者：卢毅章纪叶刘军伟王知非黄东胜宫海波何信众卫贵芬克力木·阿不都热依木周凌阳浙江省人民医院/杭州医学院附属人民医院肝胆胰外科和微创外科 310014 宁波创导三维医疗科技有限公司 315336 桐乡市第一人民医院普外科 314500 新疆维吾尔自治区人民医院微创外科乌鲁木齐830001 永嘉县中医医院普外科温州325102

目的构建3D打印腹腔镜抗反流手术干实验室模型,探讨其在外科医师手术训练中的作用。方法选取18位无手术经验的外科医师随机均分成3组,A组和C组分别采用基础硅胶模型和3D打印模型进行手术训练,B组先后采用上述两种模型进行手术训练。应... 详细信息

目的构建3D打印腹腔镜抗反流手术干实验室模型,探讨其在外科医师手术训练中的作用。方法选取18位无手术经验的外科医师随机均分成3组,A组和C组分别采用基础硅胶模型和3D打印模型进行手术训练,B组先后采用上述两种模型进行手术训练。应用手术技能客观结构化评估(OsATs)和5分制李克特(Likert)量表分别对训练表现和模型效能进行评分。组间比较采用t检验。结果所有外科医师均完成了相应手术训练和模型评分。3D打印模型和基础硅胶模型的整体印象评分为(4.28±0.67)分比(3.61±0.61)分(t=3.129,PsATs评分C组[(14.17±1.81)分]>B组[(11.00±1.76)分,t=3.074,PA组[(9.08±1.50)分,t=2.031,Pin]in,t=1.999,Pin,t=1.936,P<0.05],差异均有统计学意义。结论 3D打印腹腔镜抗反流手术训练模型较传统模型优势明显,有助于缩短学习曲线和评价手术资质。

关键词： 3D打印腹腔镜抗反流手术实验室模型手术训练

Prevalence and Prescription of Antidepressants.i. Depres.i.n with somatic Comorbidity in Asia： The Research on East Asian Psychotropic Prescription Patterns study

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2015年第7期128卷 853-858页

作者： Chao Chen Tian-Mei si Yu-Tao Xiang Gabor s Ungvari Chuan-Yue Wang Yan-Ling He Ee-Heok Kua senta Fuji Kang sim Jitendra K Trivedi Eun-Kee Chung Pichet Udomratn Kok-Yoon Chee Norman sartorius Chay-Hoon Tan Naotaka s.i.fuku Key Laboratory of Mental Health Ministry of Mental Health and Peking University Institute of Mental Health Beijing 100083 China Faculty of Health s.i.nces University of Macao Macao SAR China Department of Psychiatry Chinese University of Hong Kong Hong Kong SAR China school of Psychiatry and Clinical Neuros.i.nces University of Western Australia Perth Australia Beijing Anding Hos.i.al Capital Medical University Beijing 100088 China shanghai Mental Health Center Shanghai 200030 China Department of Psychological Medicine National University of Singapore Singapore Department of Disaster Psychiatry Fukushima Medical University Fukushima Japan ins.i.ute of Mental Health Buangkok View Singapore Department of Psychiatry C.SM. Medical University Lucknow Uttar Pradesh India Department of Psychiatry National Seoul Hospital Seoul Korea Department of Psychiatry Faculty of Medicine Prince of Songkla University Songkhla Thailand Department of Psychiatry and Mental Health Tunku Abdul Rahman Institute of Neuroscience Kuala Lumpur Hospital Kuala Lumpur Malaysia Association for the improvement of Mental Health Programs Geneva Switzerland Department of Pharmacology National University of Singapore Singapore school of Human s.i.nces Seinan Gakuin University Fukuoka Fukuoka Japan

Background： Depres.i.n is often comorbid with chronic somatic diseases. Few previous studies have inves.i.ated the prevalence of somatic diseases.i. depres.i.n or the prescription pattern of antidepressants.i. comorbidly depressed patients.i. Asia. This study aimed to inves.i.ate the prevalence of somatic comorbidity （sC） in depres.i.n and compared the prescriptions of antidepressants.i. depressed patients with and without sC. Methods： A total of 2320 patients treated with antidepressants.i. 8 Asian countries were examined, and a diagnosis was based on the international Clas.i.ication of Disease, 10^th revision. We listed 17 common chronic somatic diseases. Patients＇ socio-demographic and clinical characteris.i.s and psychotropic drug prescriptions were recorded using a standardized protocol and data collection procedure. Results： Of the patients examined, 1240 were diagnosed with depres.i.n and 30% of them （n = 375） had sC. The most common comorbid condition was diabetes （23.7%）. The patients with sC were more likely to seek help at a general hos.i.al （74.7% vs. 47.2%）, and had a higher incidence of symptoms.i.volving sadness, disturbed sleep, and poor appetite. Noradrenergic and specific serotonergic antidepressant was prescribed more for patients with sC than for those without sC （30.4% vs, 22.9%）. Conclusions： sC is common in depressed Asian patients. it is.i.portant to strengthen the recognition of depres.i.n, especially in general hos.i.als and when patients report some somatic discomfort. it is also a matter of urgency to establish evidence-based guidelines for the use of new antidepressants.i. depressed patients with sC.

关键词： Asia Depres.i.n Prescription Prevalence somatic Comorbidity

inter-ethnic marriages and severity of coronary artery disease:A multicenter study of Arabian Gulf states

在线全文

学校读者我要写书评

暂无评论

World Journal of Cardiology 2017年第4期9卷 371-377页

作者： Amin Daoulah salem Al-kaabi Amir Lotfi Mushabab Al-Murayeh s Ali Nasseri Waleed Ahmed salah N Al-Otaibi Mohamed N Alama Osama E Elkhateeb Amy J Plotkin Majed M Malak Khalid Alshali Mohamed Hamzi saleh Al Khunein Mohammed Abufayyah Alawi A Alsheikh-Ali Cardiovascular Department King Faisal Specialist Hospital and Research Center Cardiology Department Zayed Military Hospital Division of Cardiology Baystate Medical CenterTufts University School of Medicine Cardiovascular Department Armed Forces Hospital Southern Region Politecnico di Torino internal Medicine Department King Faisal Specialist Hospital and Research Center Cardiac Anesthesiology King Faisal Specialist Hospital AND Research Center Cardiology Department King Abdul Aziz University Hospital Cardiology Department King Abdullah Medical City in Holy Capital Department of Pathology Johns Hopkin University internal Medicine Department King Abdul Aziz University Hospital Anesthesia Department Prince Sultan Military Medical City College of Medicine Mohammed Bin Rashid University of Medicine and Health Sciences ins.i.ute of Cardiac s.i.nces Sheikh Khalifa Medical City

AiM To assess the association of inter-ethnic vs.i.tra-ethnic marriage with severity of coronary artery disease(CAD) in men undergoing *** We conducted a prospective multicenter,multi-ethnic,cross sectional observational study at five hos.i.als.i. saudi Arabia and the United Arab Emirates.i. which we used logis.i. regres.i.n analysis with and without adjustment for baseline differences. REsULTs Data were collected for 1068 enrolled patients undergoing coronary angiography for clinical indications during the period of April 1^(st),2013 to March 30^(th),2014. Ethnicities of spouses were available only for male patients. Of those enrolled,687 were married men and cons.i.uted the cohort for the present analysis. intra-ethnic marriages were reported in 70% and inter-ethnic marriages.i. 30%. After adjus.i.g for baseline differences.i.terethnic marriage was associated with lower odds of having significant CAD [adjusted odds ratio 0.52(95%Ci:0.33,0.81)] or multi-vessel disease(MVD) [adjusted odds ratio 0.57(95%Ci:0.37,0.86)]. The adjusted association with left main disease showed a similar trend,but was not statis.i.ally significant [adjusted odds ratio 0.74(95%Ci:0.41,1.32)]. The association between interethnic marriage and the presence of significant CAD and MVD was not modified by number of concurrent wives(P interaction > 0.05 for both).CONCLUsiON Among married men undergoing coronary angiography,inter-ethnic,as compared to intra-ethnic,marriage is associated with lower odds of significant CAD and MVD.

关键词： Arabian Gulf inter-ethnic marriage Coronary artery disease Cardiac epidemiology Coronary angiography