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Partial Fusion (F) Gene Analysis of Newcastle Disease Virus Detected in Pakistan during 2021-2022

Journal of Biosciences and Medicines 2024年第5期12卷 256-275页

作者： Muhammad Danish Mehmood Huma Anwar Ul-Haq Rauf Khalid Yasir Amin Muhammad Usman Ghani Muhammad Ismail Rabia Habib Fareeha Arshed abdul rasheed shaukat Institute of Molecular Biology & Biotechnology University of Lahore Lahore Pakistan Research & Development Division Ottoman Pharma Lahore Pakistan Quality Assurance Department Ottoman Pharma Lahore Pakistan Veterinary Research and Disease Investigation Center Abbottabad Pakistan Quality Control Department Ottoman Pharma Immuno Lahore Pakistan Production Department Ottoman Pharma Lahore Pakistan Poultry Production Department Sabir&#8217s Poultry Pvt. Ltd. Sabroso Pakistan

Newcastle disease (ND) virus is a leading threat to commercial and domestic poultry in Pakistan. The virus infects and constitutes irreversible impairment to the nervous system, damages the respiratory system, and marks severe gastrointestinal lesions leading to heavy mortality in short-living birds and substantial losses in layers and breeders. The continuous emergence and evolution of the virus made it inclined to evade the humoral response and indirectly the circumvention of artificial active immunization. Newcastle disease is caused by the orthoavula genus of the paramyxoviridae family and has shown high genetic diversity even in their genotypes while information regarding enzootic trends of the virus is scanty in Pakistan. A total of 40 tracheal samples of NDV were collected from different commercial broiler farms and 11 isolates of NDV were identified. In the current study, we determined the genetic diversity of the Newcastle disease virus based on the partial sequencing of the fusion protein gene available in the NCBI database. Genetic analysis showed that seven isolates belonged to class I genotype VII and four belonged to class II genotype II. Interestingly, two isolates had epidemiological connections with vaccine-like class II genotype II. Our findings, concerning the recent outbreaks of class I genotype VII and class II genotype II of NDV in vaccinated commercial flocks, suggest possible potential partial mutations in the fusion protein gene. Genetic diversity and formation of the new cleavage site in an important neutralizing protein of wild strain are linked with the potency of artificial active immunization and a major cause of vaccine failure.

关键词： Newcastle Disease Virus Haemagglutination Inhibition Polymerase Chain Reaction Phylogenetic Tree Mutation Analysis

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National guidelines for the diagnosis and treatment of hilar cholangiocarcinoma

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World Journal of Gastroenterology 2024年第9期30卷 1018-1042页

作者： Faisal Saud Dar Zaigham Abbas Irfan Ahmed Muhammad Atique Usman Iqbal Aujla Muhammad Azeemuddin Zeba Aziz Abu Bakar Hafeez Bhatti Tariq Ali Bangash Amna Subhan Butt Osama Tariq Butt abdul Wahab Dogar Javed Iqbal Farooqi Faisal Hanif Jahanzaib Haider Siraj Haider Syed Mujahid Hassan Adnan abdul Jabbar Aman Nawaz Khan Muhammad Shoaib Khan Muhammad Yasir Khan Amer Latif Nasir Hassan Luck Ahmad Karim Malik Kamran Rashid Sohail Rashid Mohammad Salih abdullah Saeed Amjad Salamat Ghias-un-Nabi Tayyab Aasim Yusuf Haseeb Haider Zia Ammara Naveed Department of Hepatopancreatic Biliary Surgery&Liver Transplant Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Hepatogastroenterology and Liver Transplantation Dr.Ziauddin University HospitalKarachi 75600SindhPakistan University of Aberdeen Aberdeen B243FXUnited Kingdom Department of Pathology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Gastroenterology&Hepatology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Radiology Aga Khan UniversityKarachi 74800Pakistan Department of Oncology Hameed Latif HospitalLahore 54000Pakistan Division of Hepatopancreatic Biliary Surgery&Liver Transplantation Shifa International HospitalIslamabad 44000Pakistan Department of Hepatopancreatic Biliary Surgery&Liver Transplant Shaikh Zayed Hospital and Post Graduate Medical InstituteLahore 54000Pakistan Department of Medicine Aga Khan University HospitalKarachi 74800Pakistan Department of Liver Transplant Pir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Medicine&Gastroenterology Lifecare Hospital and Research CentrePeshawar 25000Khyber PakhtunkhwaPakistan Department of Hepatopancreatobiliary&Liver Transplant Bahria International HospitalLahore 54000Pakistan Department of Surgery Hepatopancreatobiliary&Liver TransplantDow University of Health SciencesKarachi 74800Pakistan Department of Gastroenterology Hepatology&NutritionPir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Oncology Dr.Ziauddin HospitalKarachi 74800Pakistan Department of Radiology Rehman Medical InstitutePeshawar 25000Pakistan Army Liver Transplant Unit Pak Emirates Military HospitalRawalpindi 46000Pakistan Department of Gastroenterology Sindh Institute of Urology and TransplantationKarachi 75500Pakistan Rashid Nursing Home and Cancer Clinic Rashid Nursing Home and Cancer ClinicRawalpindi 46000Pakistan Department of Gastroentero

A consensus meeting of national experts from all major national hepatobiliary centres in the country was held on May 26,2023,at the Pakistan Kidney and Liver Institute&Research Centre(PKLI&RC)after initial consultations with the *** Pakistan Society for the Study of Liver Diseases(PSSLD)and PKLI&RC jointly organised this *** effort was based on a comprehensive literature review to establish national practice guidelines for hilar cholangiocarcinoma(hCCA).The consensus was that hCCA is a complex disease and requires a multidisciplinary team approach to best manage these *** coordinated effort can minimise delays and give patients a chance for curative treatment and effective *** diagnostic and staging workup includes high-quality computed tomography,magnetic resonance imaging,and magnetic resonance *** cytology or biopsy utilizing endoscopic retrograde cholangiopancreatography is a mainstay for ***,histopathologic confirmation is not always required before *** ultrasound with fine needle aspiration of regional lymph nodes and positron emission tomography scan are valuable adjuncts for *** only curative treatment is the surgical resection of the biliary tree based on the Bismuth-Corlette *** patients with unresectable hCCA can be considered for liver *** chemotherapy should be offered to patients with a high risk of *** use of preoperative biliary drainage and the need for portal vein embolisation should be based on local multidisciplinary *** with acute cholangitis can be drained with endoscopic or percutaneous biliary *** chemotherapy with cisplatin and gemcitabine has shown improved survival in patients with irresectable and recurrent hCCA.

关键词： Hilar cholangiocarcinoma Bismuth-Corlette classification Memorial Sloan Kettering Cancer Centre Staging Preoperative biliary drainage Portal vein embolisation Surgical resection Hepatectomy

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Role of foliar spray of plant growth regulators in improving photosynthetic pigments and metabolites in Plantago ovata (Psyllium) under salt stress–A field appraisal

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BIOCELL 2023年第3期47卷 523-532页

作者： abdul SAMAD KANVAL shaukat MAHMOOD-UR-REHMAN ANSARI MEREEN NIZAR NOREEN ZAHRA AMBREEN NAZ HAFIZ MUHAMMAD WALEED IQBAL ALI RAZA VLADAN PESIC IVICA DJALOVIC Department of Botany University of BalochistanQuetta08770Pakistan Department of Bioinformatics and Biotechnology Government College UniversityFaisalabad38000Pakistan Department of Botany University of AgricultureFaisalabad38040Pakistan Department of Food Science and Technology Muhammad Nawaz Shareef University of AgricultureMultan66000Pakistan Department of Agronomy Muhammad Nawaz ShareefUniversity of AgricultureMultan66000Pakistan College of Agriculture Fujian Agriculture and Forestry UniversityFuzhou350002China Department of Genetics and Plant Breeding Faculty of AgricultureUniversity of BelgradeBelgrade11000Serbia Institute of Field and Vegetable Crops National Institute of the Republic of SerbiaMaxim Gorki 30Novi Sad21000Serbia

Salinity is one of the major abiotic factors that limit the growth and productivity of *** application of plant growth regulators(PGRs)may help plants ameliorate the negative impacts of ***,a field experiment was conducted at the Botanical Garden University of Balochistan,Quetta,to explore the potential role of PGRs,i.e.,moringa leaf extract(MLE;10%),proline(PRO;1μM),salicylic acid(SA;250μM),and thiourea(TU;10 mM)in ameliorating the impacts of salinity(120 mM)on Plantago ovata,an important medicinal *** hampered plant photosynthetic pigments and metabolites but elevated oxidative ***,foliar application of PGRs enhanced photosynthetic pigments,including Chl b(21.11%),carotenoids(57.87%)except Chl a,activated the defense mechanisms by restoring and enhancing the metabolites,i.e.,soluble sugars(49.68%),soluble phenolics(33.34%),and proline(31.47%),significantly under salinity ***,foliar supplementation of PGRs under salt stress led to a decrease of about 43.02%and 43.27%in hydrogen peroxide and malondialdehyde content,***,PGRs can be recommended for improved photosynthetic efficiency and metabolite content that can help to get better yield under salt stress,with the best and most effective treatments being those of PRO and MLE to predominately ameliorate the harsh impacts of salinity.

关键词： Abiotic stress Proline Photosynthetic pigments Thiourea Salinity Salicylic acid

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Elemental composition of rice using calibration free laser induced breakdown spectroscopy

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Optoelectronics Letters 2019年第1期15卷 57-63页

作者： abdul Jabbar Mahmood Akhtar Arslan Ali shaukat Mehmood Sidra Iftikhar Muhmmad Aslam Baig Department of Physics Mirpur University of Science and Technology National Centre for Physics

In this paper, we apply laser induce breakdown spectroscopy (LIBS) to determine the elemental composition of different parts(root, stem and seed) of the rice plant and determine their weighted concentration using calibration free laser induced breakdown spectroscopy (CF-LIBS) technique. Ca, Fe and K are identified as major elements, while C, Ti, Mg, Si, Li, Ba, Sr, Cr, Na and Al as minor elements. We also detect the H-alpha line of hydrogen in the spectrum and determine the electron number density. The electron number density and its behavior as a function of laser energy, laser wavelength and the detector position are investigated. The plasma temperatures of samples are determined, and the validity of the assumption of the local thermodynamic equilibrium (LTE) is discussed.

关键词： Elemental composition calibration breakdown spectroscopy

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Analysis and Forecasting COVID-19 Outbreak in Pakistan Using Decomposition and Ensemble Model

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Computers, Materials & Continua 2021年第7期68卷 841-856页

作者： Xiaoli Qiang Muhammad Aamir Muhammad Naeem shaukat Ali Adnan Aslam Zehui Shao Institute of Computing Science and Technology Guangzhou UniversityGuangzhou510006China Department of Statistics Abdul Wali Khan UniversityMardan23200Pakistan Department of English University of MalakandChakdara188800Pakistan Department of Natural Sciences and Humanities University of Engineering and TechnologyLahore54000Pakistan

COVID-19 has caused severe health complications and produced a substantial adverse economic impact around the *** the trend of COVID-19 infections could help in executing policies to effectively reduce the number of new *** this study,we apply the decomposition and ensemble model to forecast COVID-19 confirmed cases,deaths,and recoveries in Pakistan for the upcoming month until the end of *** the decomposition of data,the Ensemble Empirical Mode Decomposition(EEMD)technique is *** decomposes the data into small components,called Intrinsic Mode Functions(IMFs).For individual IMFs modelling,we use the Autoregressive Integrated Moving Average(ARIMA)*** data used in this study is obtained from the official website of Pakistan that is publicly available and designated for COVID-19 outbreak with daily *** analyses reveal that the number of recoveries,new cases,and deaths are increasing in Pakistan *** on the selected EEMD-ARIMA model,the new confirmed cases are expected to rise from 213,470 to 311,454 by 31 July 2020,which is an increase of almost 1.46 times with a 95%prediction interval of 246,529 to 376,*** 95%prediction interval for recovery is 162,414 to 224,579,with an increase of almost two times in total from 100802 to 193495 by 31 July *** the other hand,the deaths are expected to increase from 4395 to 6751,which is almost 1.54 times,with a 95%prediction interval of 5617 to ***,the COVID-19 forecasting results of Pakistan are alarming for the next month until 31 July *** also confirm that the EEMD-ARIMA model is useful for the short-term forecasting of COVID-19,and that it is capable of keeping track of the real COVID-19 data in nearly all *** decomposition and ensemble strategy can be useful to help decision-makers in developing short-term strategies about the current number of disease occurrences until an appropriate vaccine is developed.

关键词： Analysis ARIMA COVID-19 decomposition and ensemble model forecasting

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生活废水的物化处理法

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世界环境 1986年第4期 16-19页

作者： shaukat Farooq abdul Bari 陆萄 Department of Civil Engineering. Department of Chemistry University of Petroleum and Minerals Dhahran 31261 Saudi Arabia.

引言为提高水处理中的一级沉淀效率并除去磷,目前通常采用化学沉淀法,这种方法也被单独用作废水的物理化学处理法。但作为一种完整的水处理系统与生物处理相比较,它的应用范围还是有限的,这主要是由于加入化学药物后产生了大量污泥,需... 详细信息

引言为提高水处理中的一级沉淀效率并除去磷,目前通常采用化学沉淀法,这种方法也被单独用作废水的物理化学处理法。但作为一种完整的水处理系统与生物处理相比较,它的应用范围还是有限的,这主要是由于加入化学药物后产生了大量污泥,需作进一步处理。据报道,化学沉淀法可除去80～90％的悬浮物、70～80％的BOD和80～90％的细菌。设计合理、运转正常而未加入化学药物的初级沉淀池,其去除悬浮物为50～70％、BOD为25～40％、细菌为25～75％。

关键词：高铁酸盐三种方法石灰处理浊度 COD 水质指标水池沉淀池臭氧处理悬浮体悬浮物

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