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In vitro activity of natural honey alone and in combination with curcuma starch against Rhodotorula mucilaginosa in correlation with bioactive compounds and diastase activity

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Asian Pacific Journal of Tropical Biomedicine 2013年第10期3卷 816-821页

作者： Moussa Ahmed Noureddine Djebli Saad Aissat Baghdad Khiati abdelmalek meslem Salima Bacha Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem.UniversityAlgeria

Objective:To evaluate the in vitro activity and synergism of the combinations of natural honey and curcuma starch against Rhodotorula mucilaginosa in correlation with total phenolic,flavonoid contents,and diastase ***:The Folin-Ciocalteu test was used to determine the total polyphenols content and the flavonoid content was analyzed using by the aluminum chloride *** antifungal activity of the natural honey,determined by an agar well diffusion assay and agar incorporation ***:Total phenolic content varied from(63.93±0.11)to(95.36±6.08)mg GAE/100 g honey as gallic acid *** flavonoids content varied from(5.41±0.04)to(9.94±0.54)mg CE/*** activity values were between(7.3±2.8)and(26±2.8).The zone inhibition diameter for the six honey samples without starch ranged between 6 and 20 *** starch was mixed with honey and then added to well,a zone inhibition increase diameter 7 and 21 *** percentage increase was noticed with each variety and it ranged between 5%and 62.5%.The minimal inhibitory concentrations for the six varieties of honey without starch against Rhodotorula mucilaginosa ranged between 28%and 36%(v/v).When starch was incubated with honey and then added to media,a minimal inhibitory concentration drop has been noticed with each *** ranged between 6.66%and 20%(w/v).No significant correlation was established between diastase activity and bioactive ***:The mixture of curcuma starch and honey could lead to the development of new combination antibiotics against Rhodotorula

关键词： Bioactive Compounds Diastase number Rhodotorula mucilaginosa Honey Curcuma starch

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