检索结果-南通市图书馆

Structural characteristics and the label-free detection of poly(3,4-ethylenedioxythiophene/cucurbit[7]uril)pseudorotaxane at single molecule level

引用

Nano Research 2023年第2期16卷 2728-2737页

作者： Aurica Farcas Hadjer Ouldali Corneliu Cojocaru Manuela Pastoriza-Gallego Ana-Maria Resmerita abdelghani oukhaled “Petru Poni”Institute of Macromolecular Chemistry 700487 IasiRomania CY Cergy Paris Université CNRSLAMBE95000 CergyFrance

A multi-technique approach to prove the preparation of poly(3,4-ethylenedioxythiophene/cucurbit[7]uril)pseudorotaxanes(PEDOT∙CB7-PPs)is *** docking simulation and matrix-assisted laser desorption/ionization mass spectrometry(MALDI MS)validate the complexation ability of the CB7 molecule towards 3,4-ethylenedioxythiophene(EDOT),which leads to the EDOT∙CB7 inclusion *** polymerization of EDOT∙CB7 enabled the synthesis of PEDOT∙*** water-soluble part of PEDOT∙CB7-PPs was selected,freeze-dried,and chemically ***,dynamic light scattering(DLS)has been used to study the particle size and z-potential(ZP-ζ)of PEDOT∙*** ZP-ζvalue(35 mV)evidenced that the PEDOT∙CB7-PPs formed stable water *** combining the emerging nanopore resistive pulse sensing technique(Np-RPS)and computational modeling,we identified strong interactions of PEDOT∙CB7-PPs with the aerolysin(Ael)***∙CB7-PPs behave as positive charged species,and thus trans negative bias promotes its interactions with the Ael *** computational modeling results are fully consistent with the Np-RPS detection,which also reveals strong interactions between PEDOT∙CB7-PPs and the Ael *** this study,we hope to provide new insights and a better understanding of the interactions between supramolecular complexes based on CB7 and biological entities,which is instrumental for future applications in the field of nanobiotechnology.

关键词： supramolecular encapsulation poly(3,4-ethylenedioxythiophene)(PEDOT) cucurbituril aerolysin electrical detection computational modeling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Polypeptide analysis for nanopore-based protein identification

引用

Nano Research 2022年第11期15卷 9831-9842页

作者： Mazdak Afshar Bakshloo Safia Yahiaoui Fabien Piguet Manuela Pastoriza-Gallego Régis Daniel Jérôme Mathé John J.Kasianowicz abdelghani oukhaled CY Cergy Paris Université CNRSLAMBE95000CergyFrance UniversitéParis-Saclay Univ EvryCNRSLAMBE91025Evry-CourcouronnesFrance University of South Florida Dept.of PhysicsTampaFL 33620USA Freiburg Institute for Advanced Studies Universität FreiburgFreiburg 79104Germany

Presently,proteins are identified by cleaving them with proteases,measuring the mass to charge ratio of the fragments with a mass spectrometer,and matching the fragments to segments within known proteins in *** earlier demonstrated that a nanometer-scale pore formed by aerolysin(AeL)can discriminate between,and therefore identify,three similar size proteins from their trypsin-cleaved polypeptide *** this nanopore-protease method,the protein’s identity is instead determined from characteristic ionic current blockade patterns caused by the polypeptide fragments that enter the *** results also suggested that not all of the theoretically expected cleavage products partition into the *** better understand the mechanism by which polypeptide fragments are captured,and how different polypeptides reduce the pore’s ionic current,we studied the effects of 11 identical length polypeptides with different net charges and charge *** show that under certain experimental conditions,negative,positive,and neutral polypeptides are driven into the AeL pore by the same applied voltage *** capture rate and dwell time of polypeptides in the pore depend strongly on the ionic strength,the magnitude of the applied voltage,and the net charge and charge distribution of the *** dwell time distribution depends nonmonotonically on the applied voltage(regardless of the polymer’s net charge),and its maximum value depends on the polypeptide net charge and charge *** maximum dwell time for different polypeptides does not occur at the same applied voltage amplitude,which conceivably might complicate the detection and discrimination of some polypeptide *** additional experiments,computer simulations,and artificial intelligence research are needed to better understand how to optimize the partitioning of enzymatically cleaved fragments into the AeL nanopore,the method is still capable of accurately identifying

关键词： protein identification nanopores trypsin enzymatic degradation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：