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2011 International Conference on Agricultural and Natural Resources Engineering(ANRE 2011)

作者： Tseng alh Institute of Systems Biology and Bioinformatics National Central UniversityJhongli CityTaoyuan CountyTaiwan

The development of microarrays permits us to monitor transcriptomes on a genome-wide *** validate microarray measurements,quantitative-real time-reverse transcription PCR （Q-RT-PCR） is one of the most robust and common *** new challenge in gene quantification analysis is how to explicitly incorporate statistical estimations into such *** the realm of statistical analysis,the various available methods of the probe level normalization for microarray analysis may result in distinctly different target selections as well as variation in the scores for any correlation between the microarray and Q-RT-PCR ***,we explore the differences in gene expression measurements between a short-oligonucleotide-based microarray based assay and Q-RT-PCR *** systematic analysis procedures used here focus on identifying genes with minimal variation in the microarray data,which facilitates essential internal control selection prior to Q-RT-PCR *** obtain more reliable validation measurements,it is necessary,firstly,to identify the potential internal controls to be used for the Q-RT-PCR analyses and to explore the possibility that these internal controls are suitable for general *** do this,it is necessary to examine the probe set characteristics and to rank genes with respect to variance and inter-quartile ***,correctly scoring the correlation between the microarray dataset and the Q-RT-PCR measurements is dependent on whether the raw dataset is normally distributed or not,which can be tested using the Shapiro-Wilk *** the dataset is a normally distributed,the microarray and Q-RT-PCR data comparisons should use Pearson’s or Kendall’s tau *** the dataset is not normally distributed,then Spearman’s rho test,which is the rank-based non-parametric equivalent of the more commonly used Pearson’s correlation calculation,should be ***,systematic microarray and Q-RT-PCR analyses may then be carried out to reveal th

关键词： microarray/Q-RT-PCR comparisons independent/pair-wised sample analysis Q-RT-PCR internal control.

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Pig Reproductive and Respiratory Syndrome Virus (PRRSV) Does Not Attach to Boar Sperm;It Affects Only the Velocity Pattern and the mobility Pattern

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Journal of Biosciences and medicines 2024年第4期12卷 216-228页

作者： Néstor méndez Palacios Netzi Naidí mendez Palacios felicitas Vázquez flores José Alfredo Galicia Domínguez Edgar Guadalupe Beltrán Rosas maximino méndez mendoza facultad de medicina Veterinaria and Zootecnia-Benemé rita Universidad Aut&#243noma de Puebla Puebla Mexico Independent Advisor in the Porcine Area Puebla Mexico

The purpose of the study was to evaluate the sperm viability of semen infected with PRRSV viral particles, observing the effect of the Virus on the motility of boar sperm. The work was carried out at the fmVZ-BUAP Genetics and Reproduction Laboratory. 5 stallions were used. Each sample contained 1 × 106 sperm, the PRRS virus strain was ATCC-VR-2332 (0, 102, 104 and 106 copies of RNA/mL in triplicate), it was observed daily at the CASA;Hamilton Thorne®. Cells with mT (P f evaluation with 201 ± 7.3, 167 ± 10.1, 165 ± 14.6, 134 ± 8.2 and 120 ± 8.8, respectively. The % mP between control and virus concentrations (P ≥ 0.05). The LCV on day 1 and 7 PI at 10X2 and 10X6 (P matrix, where it is observed that there is a correlation between VSL and VAP, VSL and VCL, VCL and alh, VAP with alh. There is a correlation of VSL and alh, STR and alh. In this study there were (P ≤ 0.01) in the VCL, in the concentrations (102) 162.81 ± 10.65 and (106) 177.12 ± 5.77 vs 193.04 ± 4.62 of control. This indicates that altering these parameters would be related to fertility and the PRRS virus affects the LCV. Regarding the VSL, it was observed that the sperm infected with viruses 102, 104 and 106 of 48.00 ± 3.38, 49.88 ± 1.83 and 50.55 ± 2.24 Vs. 56.66 ± 1.68 of control respectively, the control would have greater possibilities of fertilizing the oocyte. In this study, it was found (P ≤ 0.01) in the VAP with 102 of 77.26 ± 5.16, 104 with 83.35 ± 2.41 and 106 with 81.29 ± 3.14 vs the control with 90.56 ± 2.07. Regarding the alh there is (P 4 with 8.70 ± .26 and 106 with 9.64 ± 0.23 vs control 8.50 ± 0.27. The presence of different concentrations of PRRSV in boar semen induces changes in different types of sperm motility. Infection of ej

关键词： PRRSV Boar Sperms Velocity Pattern and mobility Pattern

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Impact of crystalline and amorphous iron-and aluminum hydroxides on mechanisms of phosphate adsorption and desorption

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Journal of Environmental Sciences 2018年第8期30卷 175-189页

作者： Stella Gypser florian Hirsch Anja m.Schleicher Dirk freese Brandenburg University of Technology Cottbus-Senftenberg Faculty of Environment Process Engineering Biotechnology and Chemistry Department of Soil Protection and Recultivation Brandenburg University of Technology Cottbus-Senftenberg Faculty of Environment Process Engineering Biotechnology and Chemistry Department of Geopedology and Landscape Development German Research Centre for Geosciences Section 3.1 Inorganic and Isotope Geochchemistry

fourier-transform infrared（fT-IR） spectroscopic experiments were carried out during phosphate adsorption on highly crystalline gibbsite, poorly crystalline 2-line-ferrihydrite and amorphous iron–aluminum–hydroxide mixtures in the molar ratio 1：0, 10：1, 5：1, 1：1, 1：5, 1：10 and 0：1. The OH stretching vibrational bands were utilized to analyze changes in structural and surface OH groups during adsorption, because the position of characteristic P／O vibrational bands can shift depending on reaction conditions, pH or adsorbed phosphate *** and desorption kinetics were studied at pH6 and different initial phosphate concentrations to achieve varying phosphate coverage on the mineral surfaces. for gibbsite the formation of alhPO4 and Al2HPO4 can be assumed, while for ferrihydrite, a feHPO4 or fe2PO4 complex and the precipitation of fePO4 with longer equilibration time were ***2HPO4 or a fe2PO4 surface complex was deduced for fe-hydroxides, an alh2PO4 surface complex was identified for Al-hydroxide, and both displayed either hydrogen bonds to neighboring hydroxyl groups or hydrogen bonds to outer-sphere complexes. fe：Al-hydroxide mixtures with high Al ratios showed a low phosphate desorption rate, while ferrihydrite and the fe：Al-hydroxide mixtures with high fe ratios had almost negligible desorption rates. It was concluded that within the weakly associated amorphous feO（OH） materials, fePO4 precipitated, which was bound by outer-sphere hydrogen bonds. With high Al ratios, desorption increased, which indicated weaker phosphate binding of both inner-sphere and outer-sphere complexes and hence, either no or minor quantities of precipitate. ferrihydrite showed a more rigid structure and a lower extent of precipitation compared to amorphous fe-hydroxide.

关键词： Phosphate Adsorption Desorption Surface complexation Precipitation fT-IR

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Influence of pH on formation of Taranakites

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Pedosphere 1999年第1期9卷 35-44页

作者： ZHOUJIANmIN P.m.HUANG InstituteofSoilScience theChineseAcademyofSciencesP.O.BOx821Nanjing210008China DepartmentofSoilSci

monoammonium phosphate (1 mol L-1), monopotassium phosphate (1 mol L-1) and aluminum ohloride (5×10-3 mol L-1) were used to investigate the influence of pH on the formation of NH4-taranakite and K-taranakite in systems with a high NH4H2PO4 or KH2PO4 concentration. The experimental data indicated that the reaction products of aluminum with NH4H2PO4 or KH2PO4 changed with the pH in the systems. In a pH range of 2.5 to 10.0, as the pH increased, the products in either ammonium or potassium system followed the sequence: taranakite, amorphous (NH4, K)2alh(PO4)2 .4H2O, and then crystalline (NH4, K)AlPO4OH.2H2O. However, the pH ranges of the formation of these products in ammonium system were different from potassium system. NH4-taranakite formed from pH 2.75 to PH 5.75, whereas K-taranakite formed in the pH range of 3.00 to 5.00. from the theoretical calculation by a computer program (GEOCHEm version 2.0), NH4-taranakite and K-taranakite could form at the pH from 1.50 to 8.30 and from 1.25 to 8.45,respectively. These pH ranges were much wider than the experimental results. The difference between the experimental data and theoretical data was attributed to the lack of kinetic data and/or the incompleteness and inaccuracy of the thermodynamic data in the data base of the program. The PH ranges of the formation of the taranakites indicated that the taranakites could exist in the immediate vicinity of phosphate fertilizer zone as reaction products of phosphate fertilizers with soils, especially acidic soils, resulting in the fixation of not only phosphate but also nitrogen and/or potassium in soils.

关键词： ammonium pH phosphate fertilizer potassium taranakite

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BAmO/AmmO共聚物基高能固体推进剂能量特性计算和分析

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含能材料 2015年第1期23卷 37-42页

作者：裴江峰赵凤起宋秀铎徐司雨姚二岗李猛西安近代化学研究所燃烧与爆炸重点实验室陕西西安710065

用"能量计算之星"程序(ECS)计算了以3,3-二叠氮甲基氧杂环丁烷(BAmO)与3-甲基-3-叠氮甲氧基氧杂环丁烷(AmmO)的嵌段共聚物(BAmO/AmmO)为黏合剂的高能固体推进剂的能量特性。研究了添加不同增塑剂(1,5-二叠氮-3-硝基氮杂戊烷(DIANP)、聚... 详细信息

用"能量计算之星"程序(ECS)计算了以3,3-二叠氮甲基氧杂环丁烷(BAmO)与3-甲基-3-叠氮甲氧基氧杂环丁烷(AmmO)的嵌段共聚物(BAmO/AmmO)为黏合剂的高能固体推进剂的能量特性。研究了添加不同增塑剂(1,5-二叠氮-3-硝基氮杂戊烷(DIANP)、聚叠氮缩水甘油醚(GAP)、N-丁基-2-硝酸酯乙基硝胺(BuN ENA))、氧化剂(高氯酸铵(AP)、六硝基六氮杂异伍兹烷(CL-20)及呋咱类化合物(3,4-二硝基呋咱基氧化呋咱(DNTf)、二硝基偶氮氧化二呋咱(DNAf)、二叠氮基偶氮氧化呋咱(DAAOf))和高能燃料(铝粉(Al)、三氢化铝(alh 3))对推进剂能量特性参数(比冲(ISP)、燃温(Tc)、氧系数(φ),等)的影响规律。结果表明:Bu NENA增塑的推进剂比冲高于DIANP或GAP增塑的BAm O/Am m O基推进剂。Bu NENA增塑的推进剂中,随着C L-20逐步替代AP,推进剂的Tc呈现先增后减的趋势。当CL-20含量大于55%时,推进剂比冲基本保持不变,趋于最大值。当C L-20完全替代AP,比冲下降。以D N Af代替C L-20可使推进剂比冲由2723.71 N·s·kg-1提高至2798.00 N·s·kg-1。以alh 3替代Al与CL-20,同时提高体系φ时,推进剂能量得到大幅提高。

关键词：物理化学 3,3-二叠氮甲基氧丁环与3-叠氮甲氧基-3-甲基环氧丁环共聚物(BAmO/AmmO) 黏合剂固体推进剂能量特性理论计算

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morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men

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Asian Journal of Andrology 2016年第6期18卷 831-834页

作者： Pilar Santolaria Caries Soler Pilar Recreo Teresa Carretero Araceli Bono Jose m Berne Jesus L Yaniz TECNOGAm research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences University of Zaragoza Huesca Spain Department of functionat 8iotogy and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Departament of Human Anatomy and Histolosy Faculty of Health and Sport Sciences of Huesca PLaza Universidad 3 22002 Huesca Spain TECNOGAm research group Environmental Sciences Institute (IUCA) Hospital General San Jorge de Huesca Area de Urologia Av. Martinez de Velasco 36 22004 Huesca Spain

This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis （CASA-mot）, and of sperm morphometry by computer-assisted sperm morphometry analysis （CASA-morph） using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations （slow with low alh [35.6% of all motile spermatozoa], with circular trajectories [32.0%], and rapid with high alh [32.4%]）, and three morphometric subpopulations （large-round [33.9% of all spermatozoa], elongated [32.0%], and small [34.10%]）. The distribution of kinematic sperm subpopulations was different among ejaculate fractions （P 〈 0.001）, with higher percentages of spermatozoa exhibiting slow movements with low alh in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions （P〈 0.001）, with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.

关键词： man sperm morphometry by computer-assisted sperm morphometry analysis sperm motility by computer-assisted spermanalysis sperm subpopulations split ejaculate

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Naalh_4分解后的加氢过程

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金属学报 2007年第1期43卷 96-98页

作者：方方张晶朱健陈国荣孙大林 C.m.Jensen 复旦大学材料科学系 Department of Chemistry University of Hawaii HonoluluHI 96822USA

采用同步X射线衍射、扫描电镜及颗粒度分析研究了Naalh4分解后的加氢过程．结果表明,催化剂Ti不仅可以降低Naalh4的分解温度,也可以将Na3alh6的分解温度从250℃降至160℃左右．Naalh4分解后的加氢反应理论上是可逆的,但由于分解后的产物... 详细信息

采用同步X射线衍射、扫描电镜及颗粒度分析研究了Naalh4分解后的加氢过程．结果表明,催化剂Ti不仅可以降低Naalh4的分解温度,也可以将Na3alh6的分解温度从250℃降至160℃左右．Naalh4分解后的加氢反应理论上是可逆的,但由于分解后的产物NaH和Al相互分离,尤其是由于聚集所形成的Al颗粒过大,造成Na3alh6不能全部转变成Naalh4．这也是导致在随后的吸、放氢循环过程中有效贮氢量降低的原因．进一步的实验表明,当聚集的Al颗粒平均尺寸大于2．3μm时,便不利于加氢过程的完全进行．

关键词：贮氢材料 Naalh4 加氢反应

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非典型性小叶增生与乳腺小叶原位癌同轴针吸活检:放射学-病理细致对照对进一步切除或随访的价值

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国际医学放射学杂志 2014年第1期 63-63页

作者： K.A.Atkins m.A.Cohen B.Nicholson S.Rao 袁涛 Department of radiology University of Virginia1215 Lee StOffice 3034CharlottesvilleVA Chicago

目的探讨同轴针吸活检诊断为不典型小叶增生(alh)与小叶原位癌(LCIS)时,放射学-病理细致对照的一致或不一致性能否减少手术切除。方法本研究经医院伦理

关键词：针吸活检原位癌小叶增生病理同轴放射学

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